Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,386,008 G→T 100% Q35K (CAG→AAG)  ycjY ← S9 homolog non‑peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,386,0080GT98.2% 813.2 / 4.3 244Q35K (CAG→AAG) ycjYS9 homolog non‑peptidase family protein
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base T (99/139);  total (102/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 247 total aligned reads displayed.

CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATATC  >  CP009273/1385861‑1386157
                                                                                                                                                   |                                                                                                                                                     
cTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTAt                                                                                                                                                     <  2:3861515/150‑1 (MQ=255)
cTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTAt                                                                                                                                                     >  2:1854216/1‑150 (MQ=255)
cTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTAt                                                                                                                                                     >  2:2157928/1‑150 (MQ=255)
 tAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAAATGCCTtata                                                                                                                                                    >  2:1595766/1‑150 (MQ=255)
  aaCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAc                                                                                                                                                   <  2:1690592/150‑1 (MQ=255)
   aCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACt                                                                                                                                                  <  2:905910/150‑1 (MQ=255)
   aCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCACTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACt                                                                                                                                                  <  1:1324163/150‑1 (MQ=255)
     tGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGa                                                                                                                                                >  2:4619181/1‑150 (MQ=255)
      gtcgcgGCTCGCCACCTCTTTCACCTTGATAAGATTCGTCATAAGCAATAGTTACATATCCCTTTTACTCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTATTTAACGCCCCCTCCTGTGTGAGATAACACAATTGCCTTATACTGAc                                                                                                                                               <  1:4175005/148‑1 (MQ=255)
         gcgGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACggg                                                                                                                                            <  1:466834/150‑1 (MQ=255)
           ggCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGtt                                                                                                                                          <  1:2018788/150‑1 (MQ=255)
           ggCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCGTTAACGCCCCCACCGGGGTGAGATAACAAAAATGCCGTATAATGACGGGta                                                                                                                                          >  1:3205140/1‑149 (MQ=255)
                  cgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCAGGGTGGGATAACACAATTGCCTTATACTGACGGGTTTCATCaa                                                                                                                                   >  1:3726373/1‑150 (MQ=255)
                   gccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCaaa                                                                                                                                  >  2:2109468/1‑150 (MQ=255)
                    ccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAAt                                                                                                                                 <  1:432825/150‑1 (MQ=255)
                       cTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAtttt                                                                                                                              <  2:118931/150‑1 (MQ=255)
                        tttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTg                                                                                                                             >  1:151521/1‑150 (MQ=255)
                        tttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTg                                                                                                                             <  2:125255/150‑1 (MQ=255)
                            aCCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAgg                                                                                                                         <  2:3809542/150‑1 (MQ=255)
                               ttGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGaa                                                                                                                      <  1:2622047/150‑1 (MQ=255)
                                tGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTATTTAAAGCCCCCACCGGGGTGGGATAACACAAATGCCGTATACAGACGGGGTTAATCAAATTTTGGAGGGaaa                                                                                                                     >  1:1575311/1‑150 (MQ=255)
                                 gATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAAt                                                                                                                    <  1:112271/150‑1 (MQ=255)
                                 gATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAAt                                                                                                                    <  1:293997/150‑1 (MQ=255)
                                  aTAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATa                                                                                                                   >  1:677912/1‑150 (MQ=255)
                                  aTAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATa                                                                                                                   <  1:57028/150‑1 (MQ=255)
                                   tAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAg                                                                                                                  <  2:443945/150‑1 (MQ=255)
                                   tAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAg                                                                                                                  <  2:3674846/150‑1 (MQ=255)
                                       aTGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTTTTCTTTAACGCCCCCACCGGGGTGTGATAACACAATTGCCTTATACTGACGGGTTTAATCAAATTTTGGAGGGAAATAGAtaa                                                                                                              >  1:928725/1‑148 (MQ=255)
                                       aTGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCa                                                                                                              <  1:389119/150‑1 (MQ=255)
                                       aTGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCa                                                                                                              <  2:2004908/150‑1 (MQ=255)
                                       aTGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAAATTCCTTATACTGACGGGTTTCATCAAAATTTGGAGGGAAATAGATCa                                                                                                              >  1:946951/1‑150 (MQ=255)
                                          cGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTTTTCGTTAACGCCCCCACCTGGGTGATATAACACCAATGCCATATACAGACGGGTTTCATCAAAATGTGGAGGGAAAAAGATAACTg                                                                                                           >  2:461184/1‑150 (MQ=255)
                                          cGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTg                                                                                                           >  2:134676/1‑150 (MQ=255)
                                             cATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAg                                                                                                        <  1:3014615/150‑1 (MQ=255)
                                             cATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAg                                                                                                        <  2:3403438/150‑1 (MQ=255)
                                             cATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGGTCTTTAACGCCCCCACCTGGGTGTGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGACATATATCACTGCAg                                                                                                        >  2:2406144/1‑150 (MQ=255)
                                              aTAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTTACGCCCCCACCGGGGGGAGATAACACAAATGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAAAAGATCACTGCAGa                                                                                                       >  1:525632/1‑150 (MQ=255)
                                              aTAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGa                                                                                                       >  2:3247672/1‑150 (MQ=255)
                                              aTAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGa                                                                                                       <  1:3373906/150‑1 (MQ=255)
                                              aTAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGa                                                                                                       <  1:3373800/150‑1 (MQ=255)
                                                aaGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCGGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACa                                                                                                     >  2:2972535/1‑150 (MQ=255)
                                                aaGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCGTTTACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACa                                                                                                     >  2:2809407/1‑150 (MQ=255)
                                                   cAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACGCCCCCACCAGGGTGAGATAACACAATTGCCGTATACTGACGGGTTTCATCAAATTTTGGAGGGGAATAGATCACTGCAGACAAag                                                                                                  >  1:3962749/1‑150 (MQ=255)
                                                   cAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAag                                                                                                  <  1:994234/150‑1 (MQ=255)
                                                   cAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGACTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAag                                                                                                  <  2:3896723/150‑1 (MQ=255)
                                                    aaTAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCATTAACGCCCCCACCTGGGTGAGATAAAACAATTGCCTTATACTGACGGGTTTAATCAAATTTTTGAGGGAAATAGATCACTGCAGACAAaga                                                                                                 >  2:103477/1‑150 (MQ=255)
                                                     aTAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAagag                                                                                                <  1:2409877/150‑1 (MQ=255)
                                                          gACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATAAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGt                                                                                           >  1:3795689/1‑150 (MQ=255)
                                                               aTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGat                                                                                      <  2:4044573/150‑1 (MQ=255)
                                                                tCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGatt                                                                                     <  1:1025862/150‑1 (MQ=255)
                                                                tCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGGTATTTAAAGCCCCCACCGGGGTGTGATAACACAATTGCCTTATACTGAAGGGTTTAATCAAAATTTGGTGGGAAATAGATCAATGCAGACAAAGAGATGGGTGGatt                                                                                     >  1:1612948/1‑150 (MQ=255)
                                                                 cccTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGatta                                                                                    >  2:2901619/1‑150 (MQ=255)
                                                                   cTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGattatt                                                                                  <  2:185969/150‑1 (MQ=255)
                                                                   cTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAAGTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGattatt                                                                                  <  2:185977/150‑1 (MQ=255)
                                                                    ttttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTg                                                                                 <  2:821169/150‑1 (MQ=255)
                                                                       tCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTg                                                                              <  2:2045002/150‑1 (MQ=255)
                                                                       tCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACACCCCCACCGGGGTGAGATAACACAATTGCCATATACTGACGGGTTTCATAAAATTTTGGAGGGAAATAGATAAATGCAGACAAAGAGATTGTTGGATTATTGCTg                                                                              >  2:67942/1‑150 (MQ=255)
                                                                        ccGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGt                                                                             <  1:2617738/150‑1 (MQ=255)
                                                                         cGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGtt                                                                            <  2:353754/150‑1 (MQ=255)
                                                                            cAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGt                                                                         <  2:540825/150‑1 (MQ=255)
                                                                             aGTTTTTTGGCATAGGTTCCGGCCGTTTTTTGTTTAACGCCCCCCCCAGGGGGAGATAACACAAATTCCTTATACAGACGGGTTTCATCAAATTTTGGAGGGAAATATATCACAGCCGACAAAGAGATGGGTGGGTTATTGGGGTTAGTg                                                                        >  1:1275488/1‑150 (MQ=255)
                                                                             aGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTg                                                                        <  1:210468/150‑1 (MQ=255)
                                                                             aGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTg                                                                        <  2:1819215/150‑1 (MQ=255)
                                                                             aGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTg                                                                        <  2:3935442/150‑1 (MQ=255)
                                                                                       cATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACtt                                                              <  1:2014923/150‑1 (MQ=255)
                                                                                        aTAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAAGTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACttt                                                             <  2:4512901/150‑1 (MQ=255)
                                                                                          aGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTttat                                                           <  1:1256546/150‑1 (MQ=255)
                                                                                           ggTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTttatt                                                          <  1:3052983/150‑1 (MQ=255)
                                                                                            gTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGGGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACAGCAGACAAAGAGATTGTTGGATTATTGCTGTTATTGAAGCTGACTttatta                                                         >  2:3583588/1‑150 (MQ=255)
                                                                                             ttCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTtattat                                                        <  2:409351/150‑1 (MQ=255)
                                                                                             ttCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTtattat                                                        <  1:4622979/150‑1 (MQ=255)
                                                                                              tCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTattatt                                                       <  1:4068231/150‑1 (MQ=255)
                                                                                               ccGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAATAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtc                                                      >  2:1487747/1‑150 (MQ=255)
                                                                                                cGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTTCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGAATTTATTATtca                                                     >  2:1157909/1‑150 (MQ=255)
                                                                                                cGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtca                                                     >  2:2395072/1‑150 (MQ=255)
                                                                                                 ggCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtcat                                                    <  1:2585363/150‑1 (MQ=255)
                                                                                                 ggCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtcat                                                    <  1:569622/150‑1 (MQ=255)
                                                                                                   ccGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtcatca                                                  >  1:4263170/1‑150 (MQ=255)
                                                                                                    cGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTcatcat                                                 <  2:282998/150‑1 (MQ=255)
                                                                                                    cGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTcatcat                                                 >  1:1792678/1‑150 (MQ=255)
                                                                                                      tttGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTc                                               <  1:1571633/150‑1 (MQ=255)
                                                                                                       ttGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTcc                                              <  1:2280720/150‑1 (MQ=255)
                                                                                                          ttCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGtt                                           >  2:2624607/1‑150 (MQ=255)
                                                                                                           tCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTc                                          <  1:3470978/150‑1 (MQ=255)
                                                                                                                        cACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGt                             <  1:682355/150‑1 (MQ=255)
                                                                                                                         aCCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTc                            >  1:523928/1‑150 (MQ=255)
                                                                                                                            tGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTTTCGGt                         >  1:185916/1‑150 (MQ=255)
                                                                                                                              ggTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTc                       <  2:3050318/150‑1 (MQ=255)
                                                                                                                                 gagaTAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGtt                    >  1:277462/1‑150 (MQ=255)
                                                                                                                                      aaCACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAAc               <  1:2503441/150‑1 (MQ=255)
                                                                                                                                          caATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGtt           >  2:2349603/1‑150 (MQ=255)
                                                                                                                                           aaTTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTg          <  1:3756566/150‑1 (MQ=255)
                                                                                                                                            aTTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTGTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTgg         >  2:2198746/1‑150 (MQ=255)
                                                                                                                                             ttGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGt        <  2:3137189/150‑1 (MQ=255)
                                                                                                                                             ttGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGt        <  2:2450428/150‑1 (MQ=255)
                                                                                                                                             ttGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGt        <  2:356514/150‑1 (MQ=255)
                                                                                                                                              tGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTGTTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTg       >  1:3119644/1‑150 (MQ=255)
                                                                                                                                               gCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGa      <  1:4345282/150‑1 (MQ=255)
                                                                                                                                                ccTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGat     >  2:3507260/1‑150 (MQ=255)
                                                                                                                                                   gATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATATc  <  2:2235538/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGATATC  >  CP009273/1385861‑1386157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑