Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,454,724 G→T 94.5% G472C (GGT→TGT)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,454,7240GT94.5% 1006.8 / 47.8 332G472C (GGT→TGT) paaH3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent
Reads supporting (aligned to +/- strand):  ref base G (3/15);  new base T (164/147);  total (168/164)
Fisher's exact test for biased strand distribution p-value = 3.09e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

Only 100 of 332 total aligned reads displayed.

GTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCA  >  CP009273/1454577‑1454870
                                                                                                                                                   |                                                                                                                                                  
gTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGt                                                                                                                                                  >  2:3775303/1‑150 (MQ=255)
 tGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTGCAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGtt                                                                                                                                                 >  2:1559288/1‑150 (MQ=255)
 tGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTAAAGAAACACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTGCTCGAGAGCTGtt                                                                                                                                                 >  2:244648/1‑150 (MQ=255)
   aaTTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTAt                                                                                                                                               <  1:2840536/150‑1 (MQ=255)
   aaTTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTAt                                                                                                                                               <  1:982933/150‑1 (MQ=255)
   aaTTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTAt                                                                                                                                               >  2:3041693/1‑150 (MQ=255)
       aTCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGt                                                                                                                                           <  1:4117145/150‑1 (MQ=255)
          cATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCAt                                                                                                                                        <  2:685301/150‑1 (MQ=255)
          cATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCAt                                                                                                                                        <  2:3950115/150‑1 (MQ=255)
           atatGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATa                                                                                                                                       >  2:1658715/1‑150 (MQ=255)
            tatGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATaa                                                                                                                                      <  2:524980/150‑1 (MQ=255)
              tGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAgg                                                                                                                                    <  1:175455/150‑1 (MQ=255)
                 cccACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCt                                                                                                                                 <  1:3809270/150‑1 (MQ=255)
                 cccACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTCGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCt                                                                                                                                 <  1:3934336/150‑1 (MQ=255)
                   cACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTgg                                                                                                                               >  1:1070546/1‑150 (MQ=255)
                   cACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTgg                                                                                                                               <  2:4256092/150‑1 (MQ=255)
                    aCTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGc                                                                                                                              <  1:113809/150‑1 (MQ=255)
                          cTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATg                                                                                                                        >  1:346635/1‑150 (MQ=255)
                           tGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTCCAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGc                                                                                                                       <  2:3557817/150‑1 (MQ=255)
                           tGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGc                                                                                                                       >  2:1075312/1‑150 (MQ=255)
                            ggggAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGcc                                                                                                                      <  1:3559744/150‑1 (MQ=255)
                               gAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCAt                                                                                                                   >  2:1248042/1‑150 (MQ=255)
                                aGCGCTTCTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCTCTATGTCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCTACGGGCTCTTCTTGAGAGCTGTTATGAGTCTTATGGCCTGTCAAAATTCCCAtt                                                                                                                  <  2:3190479/150‑2 (MQ=255)
                                 gcgcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGc                                                                                                                 <  2:4585267/150‑1 (MQ=255)
                                  cgcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGTCCTGGCAAAATGCCCATGCa                                                                                                                >  2:2097927/1‑150 (MQ=255)
                                  cgcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCa                                                                                                                >  2:4385936/1‑150 (MQ=255)
                                     aaCTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATg                                                                                                             >  2:2810874/1‑150 (MQ=255)
                                        ttGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTAt                                                                                                          <  2:750147/150‑1 (MQ=255)
                                           gctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATgag                                                                                                       <  2:13168/150‑1 (MQ=255)
                                              ggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAAc                                                                                                    >  1:3355417/1‑150 (MQ=255)
                                                   cGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGc                                                                                               >  2:3361964/1‑150 (MQ=255)
                                                    gAATAATAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGcc                                                                                              <  2:1781412/150‑1 (MQ=255)
                                                     aaTATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCt                                                                                             >  1:3711737/1‑150 (MQ=255)
                                                       tatTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgc                                                                                           >  2:1982431/1‑150 (MQ=255)
                                                         ttAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcgc                                                                                         >  1:2158389/1‑150 (MQ=255)
                                                          tAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATGAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGcc                                                                                        >  1:3052428/1‑150 (MQ=255)
                                                           aaGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCa                                                                                       <  2:898618/150‑1 (MQ=255)
                                                           aaGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCa                                                                                       <  2:3857911/150‑1 (MQ=255)
                                                           aaGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCa                                                                                       >  2:3968854/1‑150 (MQ=255)
                                                           aaGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCa                                                                                       <  1:2865368/150‑1 (MQ=255)
                                                              gCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAg                                                                                    >  1:4376033/1‑150 (MQ=255)
                                                               cTCCTTGAAAATCTACAGCATCACTATGGCTAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAgc                                                                                   >  2:2926298/1‑150 (MQ=255)
                                                                   ttGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCtt                                                                               <  1:2312333/150‑1 (MQ=255)
                                                                     gAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTgg                                                                             <  2:1485693/150‑1 (MQ=255)
                                                                      aaaaTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGc                                                                            >  1:926097/1‑150 (MQ=255)
                                                                           cTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGa                                                                       >  2:1620081/1‑150 (MQ=255)
                                                                           cTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGa                                                                       >  1:1003794/1‑150 (MQ=255)
                                                                            tACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGAc                                                                      >  1:1252915/1‑150 (MQ=255)
                                                                            tACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGAc                                                                      >  1:1898719/1‑150 (MQ=255)
                                                                            tACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGAc                                                                      <  1:1113127/150‑1 (MQ=255)
                                                                              cAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACAt                                                                    >  1:736950/1‑150 (MQ=255)
                                                                              cAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACAt                                                                    <  2:2054747/150‑1 (MQ=255)
                                                                               aGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACAtt                                                                   >  1:1607687/1‑150 (MQ=255)
                                                                                gCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTa                                                                  <  1:1461398/150‑1 (MQ=255)
                                                                                 cATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTAt                                                                 <  1:1530479/150‑1 (MQ=255)
                                                                                 cATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTAt                                                                 >  1:2204190/1‑150 (MQ=255)
                                                                                   tCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTAtct                                                               <  1:2023426/150‑1 (MQ=255)
                                                                                    cACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTAtctc                                                              <  1:1322877/150‑1 (MQ=255)
                                                                                    cACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTAtctc                                                              >  2:3524036/1‑150 (MQ=255)
                                                                                     aCTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCa                                                             >  1:3269743/1‑150 (MQ=255)
                                                                                      cTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCaa                                                            <  1:860697/150‑1 (MQ=255)
                                                                                             gaagaaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGa                                                     <  1:4223451/150‑1 (MQ=255)
                                                                                             gaagaaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCCGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGa                                                     >  1:3042847/1‑150 (MQ=255)
                                                                                               agaaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAg                                                   <  1:508705/150‑1 (MQ=255)
                                                                                                   cGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCtt                                               <  2:1846175/150‑1 (MQ=255)
                                                                                                    gCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCttt                                              >  1:3112700/1‑150 (MQ=255)
                                                                                                    gCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCttt                                              <  2:1686700/150‑1 (MQ=255)
                                                                                                    gCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCttt                                              >  2:4283345/1‑150 (MQ=255)
                                                                                                      tATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGc                                            >  1:104826/1‑150 (MQ=255)
                                                                                                       aTCGCCCTTGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCt                                           <  1:241738/150‑1 (MQ=255)
                                                                                                       aTCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCt                                           >  2:4166951/1‑150 (MQ=255)
                                                                                                           cccATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAg                                       >  1:3145261/1‑150 (MQ=255)
                                                                                                            ccATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGt                                      >  1:2628812/1‑150 (MQ=255)
                                                                                                            ccATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGt                                      >  2:4090049/1‑150 (MQ=255)
                                                                                                            ccATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGt                                      >  1:3223720/1‑150 (MQ=255)
                                                                                                            ccATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGt                                      <  1:2064636/150‑1 (MQ=255)
                                                                                                             cATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTg                                     >  1:1661313/1‑150 (MQ=255)
                                                                                                              aTGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGa                                    >  1:3309930/1‑150 (MQ=255)
                                                                                                                gTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGAcc                                  <  2:1346693/150‑1 (MQ=255)
                                                                                                                gTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGAcc                                  <  1:1180540/150‑1 (MQ=255)
                                                                                                                 ttCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCa                                 <  2:2248946/150‑1 (MQ=255)
                                                                                                                    aTTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGa                              >  2:3427892/1‑150 (MQ=255)
                                                                                                                    aTTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGa                              >  1:1617626/1‑150 (MQ=255)
                                                                                                                      tgctgcGCCAACGGGCGCTTCTGGAGAGCTGTTATGTGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGTGAACGATGCCTGCGCCATAGCGATTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCTTGAcc                            <  2:3267712/150‑1 (MQ=255)
                                                                                                                      tgctgcGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGAcc                            >  1:3861822/1‑150 (MQ=255)
                                                                                                                         tgcGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTc                         <  1:4538433/150‑1 (MQ=255)
                                                                                                                          gcgcCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCa                        <  1:3390056/150‑1 (MQ=255)
                                                                                                                           cgcCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCAc                       <  2:1879098/150‑1 (MQ=255)
                                                                                                                            gcCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACt                      >  2:805558/1‑150 (MQ=255)
                                                                                                                             ccAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTg                     >  2:2618116/1‑150 (MQ=255)
                                                                                                                               aaCGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGca                   >  1:3747161/1‑150 (MQ=255)
                                                                                                                               aaCGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGca                   <  2:2183950/150‑1 (MQ=255)
                                                                                                                               aaCGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGca                   >  1:999552/1‑150 (MQ=255)
                                                                                                                                aCGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGcac                  <  2:3730158/150‑1 (MQ=255)
                                                                                                                                  gggCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACaa                >  2:4034089/1‑150 (MQ=255)
                                                                                                                                       cTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCt           <  2:4506078/150‑1 (MQ=255)
                                                                                                                                           tGGAGAGCAGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAAc       <  1:1838922/150‑1 (MQ=255)
                                                                                                                                            ggAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACg      <  2:3687275/150‑1 (MQ=255)
                                                                                                                                             gagaGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACgg     <  1:615632/150‑1 (MQ=255)
                                                                                                                                               gagCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGtc   >  2:2608213/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCA  >  CP009273/1454577‑1454870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑