Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,494,358 C→A 12.0% D117Y (GAC→TAC)  ydcK ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,494,3580CA12.0% 924.6 / 66.6 263D117Y (GAC→TAC) ydcKuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (97/134);  new base A (19/13);  total (116/147)
Fisher's exact test for biased strand distribution p-value = 8.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

GACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAA  >  CP009273/1494209‑1494507
                                                                                                                                                     |                                                                                                                                                     
gACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTc                                                                                                                                                       <  1:1478717/150‑1 (MQ=255)
  cAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCAt                                                                                                                                                     <  2:1346680/150‑1 (MQ=255)
  cAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTAAt                                                                                                                                                     <  2:2725996/150‑1 (MQ=255)
   aaTACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGTCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATg                                                                                                                                                    >  1:2497112/1‑150 (MQ=255)
   aaTACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATg                                                                                                                                                    <  2:1017679/150‑1 (MQ=255)
    aTACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGa                                                                                                                                                   >  1:3282103/1‑150 (MQ=255)
     tACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGaa                                                                                                                                                  <  1:703154/150‑1 (MQ=255)
       cgcgAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATg                                                                                                                                                >  2:4040890/1‑150 (MQ=255)
         cgAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGt                                                                                                                                              <  2:189189/150‑1 (MQ=255)
          gAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTg                                                                                                                                             <  2:3585057/150‑1 (MQ=255)
          gAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTg                                                                                                                                             >  2:1533712/1‑150 (MQ=255)
            ggCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGAc                                                                                                                                           >  2:4485482/1‑150 (MQ=255)
                cTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTg                                                                                                                                       <  1:2647192/150‑1 (MQ=255)
                 tAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGt                                                                                                                                      >  1:3690855/1‑150 (MQ=255)
                 tAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGt                                                                                                                                      <  1:1540926/150‑1 (MQ=255)
                 tAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGt                                                                                                                                      >  2:2671636/1‑150 (MQ=255)
                     ccTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACt                                                                                                                                  <  2:540112/150‑1 (MQ=255)
                       ttGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTta                                                                                                                                <  2:3701713/150‑1 (MQ=255)
                       ttGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTta                                                                                                                                <  1:1776889/150‑1 (MQ=255)
                        tGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTtat                                                                                                                               <  1:119881/150‑1 (MQ=255)
                          cgcgATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGGCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTtatat                                                                                                                             <  1:3547678/150‑1 (MQ=255)
                          cgcgATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTtatat                                                                                                                             <  1:516056/150‑1 (MQ=255)
                          cgcgATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTtatat                                                                                                                             <  1:4477535/150‑1 (MQ=255)
                          cgcgATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTtatat                                                                                                                             >  1:566635/1‑150 (MQ=255)
                          cgcgATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTAATGAATGGTGACGCTGTCACTtatat                                                                                                                             >  1:2229264/1‑150 (MQ=255)
                            cgATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTAATGAATGGTGACGCTGTCACTTatatat                                                                                                                           <  2:2992726/150‑1 (MQ=255)
                             gATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATg                                                                                                                          <  2:2334954/150‑1 (MQ=255)
                             gATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATg                                                                                                                          <  1:1608055/150‑1 (MQ=255)
                              aTCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGc                                                                                                                         <  2:613300/150‑1 (MQ=255)
                                  tAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCAccc                                                                                                                     >  2:3255611/1‑150 (MQ=255)
                                  tAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCAccc                                                                                                                     >  2:2882691/1‑150 (MQ=255)
                                   aaaTTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCt                                                                                                                    <  1:4210495/150‑1 (MQ=255)
                                   aaaTTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTAATGAATGGTGACGCTGTCACTTATATATGCACCCt                                                                                                                    >  2:1518960/1‑150 (MQ=255)
                                   aaaTTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTAATGAATGGTGACGCTGTCACTTATATATGCACCCt                                                                                                                    <  1:3940021/150‑1 (MQ=255)
                                    aaTTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTg                                                                                                                   >  1:2046858/1‑150 (MQ=255)
                                     aTTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTgg                                                                                                                  <  1:4631909/150‑1 (MQ=255)
                                      tttGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACACTGTCACTTATATATGCACCCTGGc                                                                                                                 <  1:3485571/150‑1 (MQ=255)
                                       ttGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCt                                                                                                                <  1:4545083/150‑1 (MQ=255)
                                       ttGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCt                                                                                                                <  1:210516/150‑1 (MQ=255)
                                        tGGATCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTAATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTg                                                                                                               <  1:3310947/150‑1 (MQ=255)
                                           aGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGAtt                                                                                                            <  1:100282/150‑1 (MQ=255)
                                           aGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGAtt                                                                                                            <  1:2317784/150‑1 (MQ=255)
                                             cAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTc                                                                                                          >  1:1160837/1‑150 (MQ=255)
                                                gAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTAATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACt                                                                                                       <  2:1841323/150‑1 (MQ=255)
                                                 aGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACta                                                                                                      <  1:274328/150‑1 (MQ=255)
                                                  gTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACtat                                                                                                     <  1:3503442/150‑1 (MQ=255)
                                                  gTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACtat                                                                                                     >  1:1237046/1‑150 (MQ=255)
                                                   ttGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACtatt                                                                                                    <  2:221180/150‑1 (MQ=255)
                                                    tGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACtatta                                                                                                   <  2:2000544/150‑1 (MQ=255)
                                                        ggTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGa                                                                                               >  1:2794618/1‑150 (MQ=255)
                                                         gTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGAt                                                                                              <  2:1684877/150‑1 (MQ=255)
                                                            gggCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCa                                                                                           >  2:3971717/1‑150 (MQ=255)
                                                                  tAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGtt                                                                                     <  1:3631358/150‑1 (MQ=255)
                                                                   aaGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTa                                                                                    <  2:304421/150‑1 (MQ=255)
                                                                     gCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATc                                                                                  <  2:1773937/150‑1 (MQ=255)
                                                                     gCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATc                                                                                  >  2:981410/1‑150 (MQ=255)
                                                                      ccTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATcc                                                                                 <  2:2044062/150‑1 (MQ=255)
                                                                         tgtgCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGtt                                                                              <  1:4317991/150‑1 (MQ=255)
                                                                              ggcgACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTa                                                                         <  1:2953570/150‑1 (MQ=255)
                                                                              ggcgACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTa                                                                         <  1:106115/150‑1 (MQ=255)
                                                                                 gacgaTCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAc                                                                      <  2:4407433/150‑1 (MQ=255)
                                                                                 gacgaTCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAc                                                                      >  1:3981399/1‑150 (MQ=255)
                                                                                 gacgaTCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAc                                                                      <  2:3564672/150‑1 (MQ=255)
                                                                                  acgaTCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAcc                                                                     <  2:862268/150‑1 (MQ=255)
                                                                                   cgaTCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACct                                                                    >  2:2771628/1‑150 (MQ=255)
                                                                                    gaTCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACctc                                                                   >  2:81409/1‑150 (MQ=255)
                                                                                     aTCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACctct                                                                  >  2:1196127/1‑150 (MQ=255)
                                                                                     aTCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACctct                                                                  >  2:3510160/1‑150 (MQ=255)
                                                                                     aTCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTAATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACctct                                                                  >  1:929727/1‑150 (MQ=255)
                                                                                           gAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCcac                                                            <  2:2393569/150‑1 (MQ=255)
                                                                                           gAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTAATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCcac                                                            >  1:1980771/1‑150 (MQ=255)
                                                                                           gAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTAATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCcac                                                            >  1:2430313/1‑150 (MQ=255)
                                                                                                 tGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGATTGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACg                                                      <  1:1005846/150‑1 (MQ=255)
                                                                                                 tGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACg                                                      <  2:2497112/150‑1 (MQ=255)
                                                                                                  gATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGc                                                     >  1:3185721/1‑150 (MQ=255)
                                                                                                   aTCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCt                                                    <  1:1738919/150‑1 (MQ=255)
                                                                                                   aTCAATTAAGGCGTGACCAAATATTCTACATTGCACGTAAACTAATTAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCt                                                    <  2:424422/150‑1 (MQ=255)
                                                                                                     cAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTgg                                                  <  2:295645/150‑1 (MQ=255)
                                                                                                       aTTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTg                                                >  2:4224578/1‑150 (MQ=255)
                                                                                                       aTTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTg                                                >  2:56258/1‑150 (MQ=255)
                                                                                                       aTTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTAATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTg                                                >  2:1295271/1‑150 (MQ=255)
                                                                                                          aaGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCg                                             >  2:4472408/1‑150 (MQ=255)
                                                                                                           aGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCgg                                            >  1:4048043/1‑150 (MQ=255)
                                                                                                           aGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCgg                                            <  2:383556/150‑1 (MQ=255)
                                                                                                           aGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCgg                                            <  1:1545256/150‑1 (MQ=255)
                                                                                                           aGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCgg                                            <  1:583378/150‑1 (MQ=255)
                                                                                                             gCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTAATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTg                                          <  2:745727/150‑1 (MQ=255)
                                                                                                               gTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGAt                                        >  1:1867489/1‑150 (MQ=255)
                                                                                                                     aaaTATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGt                                  >  2:2096224/1‑150 (MQ=255)
                                                                                                                         atTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTa                              >  1:4512318/1‑150 (MQ=255)
                                                                                                                                aTTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGaaa                       >  1:1321937/1‑150 (MQ=255)
                                                                                                                                aTTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGaaa                       >  2:4546267/1‑150 (MQ=255)
                                                                                                                                       gTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGc                <  1:2875382/150‑1 (MQ=255)
                                                                                                                                        tAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCt               >  2:34288/1‑150 (MQ=255)
                                                                                                                                         aaaCTAATGAGTAATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTc              >  2:250215/1‑150 (MQ=255)
                                                                                                                                           aCTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTccg            >  1:183270/1‑150 (MQ=255)
                                                                                                                                            cTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTccgc           >  1:4346852/1‑150 (MQ=255)
                                                                                                                                                  aGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACg     <  1:1658423/150‑1 (MQ=255)
                                                                                                                                                     cATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCaa  <  1:1275878/150‑1 (MQ=255)
                                                                                                                                                     cATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCaa  <  1:2874853/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAA  >  CP009273/1494209‑1494507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑