Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,529,506 C→T 14.3% G204S (GGC→AGC)  yddE ← PhzC‑PhzF family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,529,5060CT14.3% 778.9 / 92.8 266G204S (GGC→AGC) yddEPhzC‑PhzF family protein
Reads supporting (aligned to +/- strand):  ref base C (100/128);  new base T (12/26);  total (112/154)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

CCTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAA  >  CP009273/1529358‑1529655
                                                                                                                                                    |                                                                                                                                                     
ccTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCt                                                                                                                                                      <  1:12358/150‑1 (MQ=255)
 cTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTg                                                                                                                                                     >  2:2613840/1‑150 (MQ=255)
 cTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTg                                                                                                                                                     >  2:1276295/1‑150 (MQ=255)
       cacGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGa                                                                                                                                               <  2:4445085/150‑1 (MQ=255)
       cacGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGa                                                                                                                                               >  1:1559199/1‑150 (MQ=255)
         cGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATc                                                                                                                                             >  1:4423200/1‑150 (MQ=255)
          gCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCt                                                                                                                                            <  1:1441709/150‑1 (MQ=255)
            aaCACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTgg                                                                                                                                          >  2:3378565/1‑150 (MQ=255)
            aaCACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTgg                                                                                                                                          >  2:2747616/1‑150 (MQ=255)
             acacGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGa                                                                                                                                         <  1:932704/150‑1 (MQ=255)
                 gTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACgg                                                                                                                                     <  1:4529463/150‑1 (MQ=255)
                  ttGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACggg                                                                                                                                    <  2:1204959/150‑1 (MQ=255)
                   tGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGga                                                                                                                                   >  1:4142552/1‑150 (MQ=255)
                    gCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGCTTTCGTTTTTGCTTGGGCGGATCTGGAACGGgaa                                                                                                                                  <  2:2222880/150‑1 (MQ=255)
                     ccATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGgaag                                                                                                                                 <  2:874897/150‑1 (MQ=255)
                       aTCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGgaagaa                                                                                                                               >  1:3533243/1‑150 (MQ=255)
                        tCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGaaa                                                                                                                              <  2:3945936/150‑1 (MQ=255)
                          gTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAAcc                                                                                                                            <  1:698163/150‑1 (MQ=255)
                          gTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAAcc                                                                                                                            <  1:3507968/150‑1 (MQ=255)
                           tGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCa                                                                                                                           <  1:3695659/150‑1 (MQ=255)
                           tGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCa                                                                                                                           <  1:3162238/150‑1 (MQ=255)
                           tGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCa                                                                                                                           >  2:2968122/1‑150 (MQ=255)
                            ggggCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCAt                                                                                                                          >  1:2710723/1‑150 (MQ=255)
                             gggCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCAtt                                                                                                                         >  1:2280518/1‑150 (MQ=255)
                                cAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCa                                                                                                                      <  2:164466/150‑1 (MQ=255)
                                   taagTTGTGATGTAACAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTTCTTGGGCGTATCTGGAACGTGAAGAAACCATTGCAGcc                                                                                                                   >  2:2272626/4‑150 (MQ=255)
                                     cGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCaa                                                                                                                 <  1:572841/150‑1 (MQ=255)
                                       ttGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATc                                                                                                               <  2:1722782/150‑1 (MQ=255)
                                        tgtgATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCt                                                                                                              <  1:2609132/150‑1 (MQ=255)
                                          tgATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCttt                                                                                                            <  2:2990068/150‑1 (MQ=255)
                                          tgATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCttt                                                                                                            <  2:268305/150‑1 (MQ=255)
                                           gATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCtttt                                                                                                           >  2:3771309/1‑150 (MQ=255)
                                           gATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCtttt                                                                                                           <  1:2282182/150‑1 (MQ=255)
                                            aTGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCttttt                                                                                                          >  2:108829/1‑150 (MQ=255)
                                             tGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTg                                                                                                         <  1:4375355/150‑1 (MQ=255)
                                             tGTACCAACCATGCACACATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGTTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCGAATCTTTTTg                                                                                                         <  2:1570589/150‑1 (MQ=255)
                                               tACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCt                                                                                                       >  1:1922928/1‑150 (MQ=255)
                                               tACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCt                                                                                                       <  1:827903/150‑1 (MQ=255)
                                               tACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCt                                                                                                       >  2:25426/1‑150 (MQ=255)
                                                 ccaaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGa                                                                                                     >  1:305629/1‑150 (MQ=255)
                                                  caaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGAt                                                                                                    <  2:1735431/150‑1 (MQ=255)
                                                  caaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGAt                                                                                                    >  2:4051406/1‑150 (MQ=255)
                                                  caaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGAt                                                                                                    >  1:995269/1‑150 (MQ=255)
                                                  caaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGAt                                                                                                    <  1:1127234/150‑1 (MQ=255)
                                                  caaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGAt                                                                                                    <  1:3826093/150‑1 (MQ=255)
                                                   aaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATa                                                                                                   >  1:620983/1‑150 (MQ=255)
                                                   aaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATa                                                                                                   <  2:2851880/150‑1 (MQ=255)
                                                        tGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGt                                                                                              <  1:3076565/150‑1 (MQ=255)
                                                          cACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCa                                                                                            <  2:4547874/150‑1 (MQ=255)
                                                           aCCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAg                                                                                           >  1:2490310/1‑150 (MQ=255)
                                                           aCCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAg                                                                                           >  2:1442857/1‑150 (MQ=255)
                                                             ccATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAgcg                                                                                         <  1:1682120/150‑1 (MQ=255)
                                                                 cGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCAtt                                                                                     <  1:3371937/150‑1 (MQ=255)
                                                                      cATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGt                                                                                <  2:4255421/150‑1 (MQ=255)
                                                                       aTTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTc                                                                               >  2:2333618/1‑150 (MQ=255)
                                                                       aTTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTc                                                                               >  2:2654487/1‑150 (MQ=255)
                                                                        ttGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCa                                                                              >  1:92823/1‑150 (MQ=255)
                                                                        ttGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCa                                                                              <  2:4173092/150‑1 (MQ=255)
                                                                        ttGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCa                                                                              <  1:53594/150‑1 (MQ=255)
                                                                          ggCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAgg                                                                            <  2:1901924/150‑1 (MQ=255)
                                                                            cATTGCCAGTCACTGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCg                                                                          <  1:4467924/150‑1 (MQ=255)
                                                                            cATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCg                                                                          <  2:1635691/150‑1 (MQ=255)
                                                                            cATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCg                                                                          >  1:1150339/1‑150 (MQ=255)
                                                                            cATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCg                                                                          >  1:3470157/1‑150 (MQ=255)
                                                                             aTTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGa                                                                         >  2:3986965/1‑150 (MQ=255)
                                                                              ttGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGaa                                                                        <  1:3902104/150‑1 (MQ=255)
                                                                              ttGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGaa                                                                        >  2:648463/1‑150 (MQ=255)
                                                                                 ccAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAgg                                                                     <  2:806120/150‑1 (MQ=255)
                                                                                  cAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAggg                                                                    <  1:4035169/150‑1 (MQ=255)
                                                                                  cAGTCACCGGATCGTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAggg                                                                    >  1:2665514/1‑150 (MQ=255)
                                                                                    gTCACCGGATCGTCCTCAATCCCAATCGCAGGCGTGTACATGCGACCATGGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGAGCATTAAGGTCAGGCGAAAGGGCg                                                                  <  2:1137627/150‑1 (MQ=255)
                                                                                     tCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGt                                                                 <  1:3791312/150‑1 (MQ=255)
                                                                                     tCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGt                                                                 >  2:2059120/1‑150 (MQ=255)
                                                                                       aCCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCg                                                               >  2:501546/1‑150 (MQ=255)
                                                                                         cGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGat                                                             <  2:3344249/150‑1 (MQ=255)
                                                                                           gATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGatat                                                           <  2:3421396/150‑1 (MQ=255)
                                                                                               ttCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCAc                                                       <  2:2193532/150‑1 (MQ=255)
                                                                                                   acaATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTcc                                                   <  2:510384/150‑1 (MQ=255)
                                                                                                       tACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGtt                                               <  1:3217934/150‑1 (MQ=255)
                                                                                                        aCCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACGTCCGGttt                                              >  2:280045/1‑150 (MQ=255)
                                                                                                         ccAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTc                                             <  2:665837/150‑1 (MQ=255)
                                                                                                          cAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCa                                            <  2:1353794/150‑1 (MQ=255)
                                                                                                           aaTCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAg                                           >  1:1958256/1‑150 (MQ=255)
                                                                                                              cGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTgg                                        >  2:3176656/1‑150 (MQ=255)
                                                                                                              cGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTgg                                        >  2:1817985/1‑150 (MQ=255)
                                                                                                               gCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTggg                                       >  1:2950757/1‑150 (MQ=255)
                                                                                                                       gaACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCAc                               <  2:2718307/150‑1 (MQ=255)
                                                                                                                        aaCATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACt                              <  2:2315934/150‑1 (MQ=255)
                                                                                                                           aTGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACtttt                           <  2:2037811/150‑1 (MQ=255)
                                                                                                                           aTGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACtttt                           <  2:1925/150‑1 (MQ=255)
                                                                                                                            tGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTg                          <  1:2827306/150‑1 (MQ=255)
                                                                                                                                   aTCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTcc                   >  1:4610755/1‑150 (MQ=255)
                                                                                                                                     cGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtg                 <  2:1388352/150‑1 (MQ=255)
                                                                                                                                      ggTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgt                <  2:1979309/150‑1 (MQ=255)
                                                                                                                                      ggTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgt                >  2:2124936/1‑150 (MQ=255)
                                                                                                                                          tCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTgttgtt            >  1:324798/1‑150 (MQ=255)
                                                                                                                                          tCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTgttgtt            <  1:209798/150‑1 (MQ=255)
                                                                                                                                              ttttGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCa        <  1:2371568/150‑1 (MQ=255)
                                                                                                                                               tttGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAc       <  1:2840534/150‑1 (MQ=255)
                                                                                                                                                    cTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGaa  >  1:688855/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAA  >  CP009273/1529358‑1529655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑