Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,591,853 C→A 49.6% pseudogene (392/5323 nt) yneO ← pseudogene, AidA homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,591,8530CA49.6% 144.2 / inf 304pseudogene (392/5323 nt)yneOpseudogene, AidA homolog
Reads supporting (aligned to +/- strand):  ref base C (84/69);  new base A (75/75);  total (159/145)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 307 total aligned reads displayed.

TGGCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTC  >  CP009273/1591704‑1592001
                                                                                                                                                     |                                                                                                                                                    
tGGCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATc                                                                                                                                                      <  2:1676593/150‑1 (MQ=255)
 ggCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCt                                                                                                                                                     <  1:3925105/150‑1 (MQ=255)
   cgacgaTTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTa                                                                                                                                                   >  2:499583/1‑150 (MQ=255)
      cgaTTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAAt                                                                                                                                                >  1:4465188/1‑150 (MQ=255)
        aTTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAg                                                                                                                                              >  1:4122310/1‑150 (MQ=255)
        aTTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAg                                                                                                                                              >  1:2009230/1‑150 (MQ=255)
           gAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCt                                                                                                                                           <  1:2089227/150‑1 (MQ=255)
             aCTCTTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTcc                                                                                                                                         <  2:4497470/150‑1 (MQ=255)
              cTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCa                                                                                                                                        >  1:3674819/1‑150 (MQ=255)
              cTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCa                                                                                                                                        <  2:893730/150‑1 (MQ=255)
               tCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCAt                                                                                                                                       <  2:267431/150‑1 (MQ=255)
                 gTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCg                                                                                                                                     <  2:2171563/150‑1 (MQ=255)
                  tGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGa                                                                                                                                    <  1:4564540/150‑1 (MQ=255)
                     cGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGt                                                                                                                                 <  2:3308022/150‑1 (MQ=255)
                     cGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGt                                                                                                                                 <  1:3210717/150‑1 (MQ=255)
                      gTAACCCTTATCCTTAATATTTAATGAACCTGTGCCATAGCGACCTATTTCTAATAATTCGGTCGTAAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGGCTCCTGTATAATAACCTAATTTTAAATTGCCTCCATCGACGTg                                                                                                                                <  2:2270184/150‑1 (MQ=255)
                      gTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTg                                                                                                                                <  2:4125940/150‑1 (MQ=255)
                        aaCCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGAc                                                                                                                              >  2:2986622/1‑150 (MQ=255)
                         aCCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGAcc                                                                                                                             >  1:247647/1‑150 (MQ=255)
                          cccTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGAccc                                                                                                                            >  2:3164333/1‑150 (MQ=255)
                           ccTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCt                                                                                                                           <  1:607355/150‑1 (MQ=255)
                           ccTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCt                                                                                                                           >  2:3813797/1‑150 (MQ=255)
                            cTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCtt                                                                                                                          >  2:1327857/1‑150 (MQ=255)
                              tATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCt                                                                                                                        >  1:25543/1‑150 (MQ=255)
                                tCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGt                                                                                                                      >  1:656921/1‑150 (MQ=255)
                                 ccGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGtt                                                                                                                     >  2:2792137/1‑150 (MQ=255)
                                     aaTATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGAGACCCTTCTGTTTAat                                                                                                                 >  1:2566394/1‑150 (MQ=255)
                                       tatTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAatat                                                                                                               >  1:1127474/1‑150 (MQ=255)
                                       tatTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAatat                                                                                                               <  1:3800344/150‑1 (MQ=255)
                                       tatTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAatat                                                                                                               <  2:3588426/150‑1 (MQ=255)
                                        atTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATAtt                                                                                                              <  1:1834996/150‑1 (MQ=255)
                                         tttAATGAACCTGTGCCATAGCTACCTATTTCGAATAAGTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTACTCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATAGTc                                                                                                             <  2:4094042/150‑1 (MQ=255)
                                           tAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAg                                                                                                           >  2:2896170/1‑150 (MQ=255)
                                             aTGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCg                                                                                                         >  2:3529390/1‑150 (MQ=255)
                                             aTGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCg                                                                                                         >  1:52904/1‑150 (MQ=255)
                                             aTGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCATTCTGTTTAATATTCAGCg                                                                                                         >  2:1210556/1‑150 (MQ=255)
                                              tGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGt                                                                                                        <  1:2213069/150‑1 (MQ=255)
                                              tGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCGTCTGTTTAATATTCAGCGt                                                                                                        >  2:806689/1‑150 (MQ=255)
                                                aaCCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTcc                                                                                                      <  2:3659966/150‑1 (MQ=255)
                                                 aacTGTGCCATAGCAAACTATTTCGAATAATTAGGTCGTCAGAACAGAGTCCACTCCAACAACATTGACCGTCCCGACGCCTCCAGTCGAAGAACCTAATCTTACATAGCCTCCATCGACTTGAACATTATGTTTAATATTCAGCGTcca                                                                                                     >  2:2243079/3‑149 (MQ=255)
                                                  ccTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTcccc                                                                                                    <  1:2827989/150‑1 (MQ=255)
                                                     gtgCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGtt                                                                                                 <  1:408075/150‑1 (MQ=255)
                                                     gtgCCATAGATAACTATTTCGAATTATTCGGTCGTCATAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGAACCTTCTGTTTAATATTCAGCGTACCCGtt                                                                                                 <  2:819347/150‑1 (MQ=255)
                                                      tgCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTc                                                                                                >  2:1178422/1‑150 (MQ=255)
                                                      tgCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTc                                                                                                >  1:1265216/1‑150 (MQ=255)
                                                       gCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTcc                                                                                               <  2:33535/150‑1 (MQ=255)
                                                       gCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTcc                                                                                               <  2:341106/150‑1 (MQ=255)
                                                       gCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTcc                                                                                               <  2:2933924/150‑1 (MQ=255)
                                                        ccATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCACCATTGACCGTCCCGACGCCTCCTTTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGAGCCTTATGTTTAATATTCAGCGTCCCCGTTCCg                                                                                              <  1:2731140/150‑1 (MQ=255)
                                                         cATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCgg                                                                                             <  1:4348229/150‑1 (MQ=255)
                                                           tAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGAt                                                                                           >  2:3794666/1‑150 (MQ=255)
                                                            aGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGAtt                                                                                          >  2:4407246/1‑150 (MQ=255)
                                                             gCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTg                                                                                         >  1:2423094/1‑150 (MQ=255)
                                                               tACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGAc                                                                                       <  1:278595/150‑1 (MQ=255)
                                                               tACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGAc                                                                                       >  1:4635935/1‑150 (MQ=255)
                                                                 ccTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTAAGCGTCCCCGTTCCGGATTGAccc                                                                                     <  2:143449/150‑1 (MQ=255)
                                                                 ccTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGAccc                                                                                     >  2:2349358/1‑150 (MQ=255)
                                                                   tATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCcac                                                                                   >  1:733668/1‑150 (MQ=255)
                                                                    aTTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTAAGCGTCCCCGTTCCGGATTGACCcaca                                                                                  >  2:3590860/1‑150 (MQ=255)
                                                                      ttCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACAtt                                                                                <  1:3426637/150‑1 (MQ=255)
                                                                      ttCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACAtt                                                                                <  1:156520/150‑1 (MQ=255)
                                                                       tCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACAttt                                                                               <  2:3885150/150‑1 (MQ=255)
                                                                       tCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACAttt                                                                               <  1:1056026/150‑1 (MQ=255)
                                                                           ataatTCGGTCGACAGAACAGAGTCCTCTCCCTCAACATTGACCGTCACGACGCCTCCTGTCGAAGAAACTAATATTAAATAGCCTCCATCGACGTGACCAGACTGTTTAATATTCAGCGTCCACGTACCGGATTGAACCACATTTAAAg                                                                           >  2:2471024/1‑150 (MQ=255)
                                                                                tCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCtt                                                                      <  1:3176696/150‑1 (MQ=255)
                                                                                 cGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTg                                                                     <  1:4312655/150‑1 (MQ=255)
                                                                                  ggTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGc                                                                    >  1:1212915/1‑150 (MQ=255)
                                                                                         aGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGACTCCATCGACGTGACCATTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTAttt                                                             >  1:1604420/1‑150 (MQ=255)
                                                                                              agagTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCt                                                        <  1:3284548/150‑1 (MQ=255)
                                                                                              agagTCCTCTCCCACAACATTGACCGTCCCGACGCCTCNTTTCGAAGAACCTAATATTAAATAGCCACCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCt                                                        <  2:1798113/150‑1 (MQ=255)
                                                                                                 gTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATc                                                     <  2:4190491/150‑1 (MQ=255)
                                                                                                  tCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCa                                                    >  2:3458617/1‑150 (MQ=255)
                                                                                                    ctctCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGATATCATa                                                  >  1:3976231/1‑150 (MQ=255)
                                                                                                       tcCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACaa                                               >  2:3391078/1‑150 (MQ=255)
                                                                                                         ccTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATc                                             <  2:135480/150‑1 (MQ=255)
                                                                                                         ccTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATc                                             <  2:1668641/150‑1 (MQ=255)
                                                                                                          cTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCg                                            >  1:4601321/1‑150 (MQ=255)
                                                                                                             aaCATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCa                                         <  1:3845606/150‑1 (MQ=255)
                                                                                                                aTTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGt                                      >  2:594155/1‑150 (MQ=255)
                                                                                                                  tGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGc                                    >  2:2395191/1‑150 (MQ=255)
                                                                                                                   gACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGcc                                   <  2:3003702/150‑1 (MQ=255)
                                                                                                                    aCCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCa                                  <  2:1737636/150‑1 (MQ=255)
                                                                                                                            gACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAAc                          >  1:1976455/1‑150 (MQ=255)
                                                                                                                            gACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAAc                          >  1:3018117/1‑150 (MQ=255)
                                                                                                                                 ctcctGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTaa                     >  1:4246390/1‑150 (MQ=255)
                                                                                                                                 ctcctGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTaa                     >  2:2488657/1‑150 (MQ=255)
                                                                                                                                    ctGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACgg                  <  1:1613024/150‑1 (MQ=255)
                                                                                                                                     tGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGt                 <  1:3550447/150‑1 (MQ=255)
                                                                                                                                        cGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGcc              >  2:638690/1‑150 (MQ=255)
                                                                                                                                         gaagaaCCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGccc             <  2:197874/150‑1 (MQ=255)
                                                                                                                                          aagaaCCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCct            <  2:909319/150‑1 (MQ=255)
                                                                                                                                            gaaCCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCctct          <  1:2124059/150‑1 (MQ=255)
                                                                                                                                             aaCCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTg         <  1:2318215/150‑1 (MQ=255)
                                                                                                                                              aCCTAATATTAATTAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCTTTCCGGATTGACCCTCATTTATAGGCCTTGCATTATTTCCGCTTTCATACAATCGCGAGGTGCCACCCAAAACATTAACGGTGCCCTCTGa        <  2:1498291/150‑1 (MQ=255)
                                                                                                                                               ccTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATGTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAg       >  2:3432131/1‑150 (MQ=255)
                                                                                                                                                cTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGt      <  1:4042648/150‑1 (MQ=255)
                                                                                                                                                 tAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTc     <  2:4209438/150‑1 (MQ=255)
                                                                                                                                                  aaTATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTcc    <  2:770262/150‑1 (MQ=255)
                                                                                                                                                    tCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTc  >  1:2952483/1‑150 (MQ=255)
                                                                                                                                                    tCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTc  >  2:2047979/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGGCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTC  >  CP009273/1591704‑1592001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑