Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 10,444 G→T 100% F17L (TTC→TTA)  satP ← succinate‑acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927310,4440GT98.8% 880.8 / 1.2 254F17L (TTC→TTA) satPsuccinate‑acetate transporter
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (118/133);  total (121/133)
Fisher's exact test for biased strand distribution p-value = 1.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 254 total aligned reads displayed.

TAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGG  >  CP009273/10297‑10587
                                                                                                                                                   |                                                                                                                                               
 aaaCCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAg                                                                                                                                              <  2:446769/150‑1 (MQ=255)
   aCCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGcc                                                                                                                                            >  1:4243378/1‑150 (MQ=255)
     cGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCa                                                                                                                                          >  2:2736301/1‑150 (MQ=255)
          gtgtTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAgg                                                                                                                                     >  2:4591057/1‑150 (MQ=255)
            gtTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGcc                                                                                                                                   >  2:2287693/1‑150 (MQ=255)
                 cTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCg                                                                                                                              <  2:1260626/150‑1 (MQ=255)
                    ttttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTg                                                                                                                           <  2:131218/150‑1 (MQ=255)
                     tttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGc                                                                                                                          >  1:3324472/1‑150 (MQ=255)
                      ttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGcc                                                                                                                         <  1:519437/150‑1 (MQ=255)
                       tGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCg                                                                                                                        <  2:3937942/150‑1 (MQ=255)
                       tGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCg                                                                                                                        <  2:3220072/150‑1 (MQ=255)
                       tGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCg                                                                                                                        <  2:2588356/150‑1 (MQ=255)
                        gTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCgg                                                                                                                       <  1:2740208/150‑1 (MQ=255)
                            tCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTa                                                                                                                   <  2:2534791/150‑1 (MQ=255)
                            tCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTa                                                                                                                   >  1:1265962/1‑150 (MQ=255)
                               agcagACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGcc                                                                                                                <  2:4272762/150‑1 (MQ=255)
                               agcagACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGcc                                                                                                                <  1:4612175/150‑1 (MQ=255)
                               agcagACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGcc                                                                                                                >  2:3682948/1‑150 (MQ=255)
                                gcagACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCa                                                                                                               >  1:3715500/1‑150 (MQ=255)
                                 cagACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGACCCAGCGGTGCCGGATTAGCCaa                                                                                                              >  2:2169593/1‑150 (MQ=255)
                                  agACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAAc                                                                                                             >  2:2639701/1‑150 (MQ=255)
                                      cAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAg                                                                                                         >  1:2408830/1‑150 (MQ=255)
                                      cAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAg                                                                                                         >  2:3815504/1‑150 (MQ=255)
                                        gCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAgtg                                                                                                       >  1:3288676/1‑150 (MQ=255)
                                          aaaaaTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGtt                                                                                                     <  2:2545403/150‑1 (MQ=255)
                                          aaaaaTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGtt                                                                                                     <  1:1992662/150‑1 (MQ=255)
                                           aaaaTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTg                                                                                                    <  1:3120265/150‑1 (MQ=255)
                                           aaaaTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTg                                                                                                    >  2:1093527/1‑150 (MQ=255)
                                             aaTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGcc                                                                                                  <  1:82495/150‑1 (MQ=255)
                                             aaTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGcc                                                                                                  <  1:4048655/150‑1 (MQ=255)
                                              aTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGccc                                                                                                 <  2:2145459/150‑1 (MQ=255)
                                               tttGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCa                                                                                                <  1:2645602/150‑1 (MQ=255)
                                               tttGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCa                                                                                                <  1:1573436/150‑1 (MQ=255)
                                                ttGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCAt                                                                                               <  1:2155529/150‑1 (MQ=255)
                                                 tGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATa                                                                                              <  2:2976694/150‑1 (MQ=255)
                                                     cgATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTc                                                                                          <  2:1067443/150‑1 (MQ=255)
                                                       aTGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCt                                                                                        <  1:1127611/150‑1 (MQ=255)
                                                         gccgccGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCa                                                                                      <  2:4027586/150‑1 (MQ=255)
                                                         gccgccGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCa                                                                                      >  2:3828182/1‑150 (MQ=255)
                                                          ccgccgTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCaa                                                                                     <  1:1901911/150‑1 (MQ=255)
                                                          ccgccgTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCAAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCaa                                                                                     <  2:4413587/150‑1 (MQ=255)
                                                              cgTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAat                                                                                 <  1:2840217/150‑1 (MQ=255)
                                                                 aGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcat                                                                              >  1:478686/1‑150 (MQ=255)
                                                                  gAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatc                                                                             <  2:1811388/150‑1 (MQ=255)
                                                                    aaaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatcat                                                                           >  2:4180511/1‑150 (MQ=255)
                                                                     aaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatcatc                                                                          >  1:1247047/1‑150 (MQ=255)
                                                                      aTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCg                                                                         <  1:1547105/150‑1 (MQ=255)
                                                                        gCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaa                                                                       >  2:879178/1‑150 (MQ=255)
                                                                        gCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaa                                                                       <  1:2899438/150‑1 (MQ=255)
                                                                         cccATGGCAAGAATAATACCGTACAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaat                                                                      <  1:1874263/150‑1 (MQ=255)
                                                                           cATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatga                                                                    >  2:2267667/1‑150 (MQ=255)
                                                                            aTGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatgaa                                                                   >  1:1689487/1‑150 (MQ=255)
                                                                                cAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGt                                                               >  1:807534/1‑150 (MQ=255)
                                                                                 aattataataCCGTCCAGACCGTAATTACCCCAGTTGTTCAGGTTAAGCCGAATTGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTg                                                              <  2:2856246/146‑1 (MQ=255)
                                                                                 aaGAATAATACCGTCCAGAGCGAAATAACCNACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTg                                                              >  1:4281269/1‑150 (MQ=255)
                                                                                  aGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGa                                                             <  1:1042552/150‑1 (MQ=255)
                                                                                       aataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAaataa                                                        >  1:2469875/1‑150 (MQ=255)
                                                                                       aataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAaataa                                                        >  1:2469941/1‑150 (MQ=255)
                                                                                         taCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAAtt                                                      >  2:2967859/1‑150 (MQ=255)
                                                                                          aCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAAttt                                                     <  1:1976584/150‑1 (MQ=255)
                                                                                            cGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTcc                                                   <  2:1476366/150‑1 (MQ=255)
                                                                                            cGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTcc                                                   >  1:3659201/1‑150 (MQ=255)
                                                                                             gTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTccc                                                  <  1:4343538/150‑1 (MQ=255)
                                                                                             gTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTccc                                                  >  1:2634800/1‑150 (MQ=255)
                                                                                             gTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGACCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTccc                                                  >  2:4406100/1‑150 (MQ=255)
                                                                                              tCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCt                                                 >  1:1329190/1‑150 (MQ=255)
                                                                                              tCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCt                                                 >  1:3411138/1‑150 (MQ=255)
                                                                                              tCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCt                                                 >  1:587863/1‑150 (MQ=255)
                                                                                              tCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCt                                                 >  2:4528707/1‑150 (MQ=255)
                                                                                                   agCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATa                                            >  2:317150/1‑150 (MQ=255)
                                                                                                     cGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAAc                                          >  1:2369564/1‑150 (MQ=255)
                                                                                                     cGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAAc                                          <  1:737088/150‑1 (MQ=255)
                                                                                                     cGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAAc                                          <  1:3741649/150‑1 (MQ=255)
                                                                                                      gAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACt                                         >  2:2059951/1‑150 (MQ=255)
                                                                                                      gAAATAACCCACGGTGTGCAGGTTAAGCAGAATGGTTGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAAACATCATCGAATGAATGGTGAAATAATTTCACTGAGTAACt                                         >  2:1542500/1‑150 (MQ=255)
                                                                                                       aaaTAACCCACGTTGTGCAGGTTAATCAGAATGGTGGTCATGCATAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGATATATTTTCCCTGAATATCTg                                        <  2:3033166/150‑1 (MQ=255)
                                                                                                       aaaTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTg                                        >  2:219869/1‑150 (MQ=255)
                                                                                                       aaaTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTg                                        <  2:3033249/150‑1 (MQ=255)
                                                                                                       aaaTAACCCACGGTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTg                                        <  2:4226421/150‑1 (MQ=255)
                                                                                                        aaTAACCCTCGTTGTGGTGGTTAAGCTGAATGGTGGTCATGCCTAAGCCGTTCAGGCCCAGCGGTGCCGGATTAGCCTTCTTAGTGTTGCCCATAATTCCTCAACAATCATCATCGAATGAATGGTGATATATTTTCCCTGAATAACTGt                                       <  2:1624328/150‑1 (MQ=255)
                                                                                                             cccACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAgtgt                                  <  2:4379720/150‑1 (MQ=255)
                                                                                                               cACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGttt                                >  1:2945293/1‑150 (MQ=255)
                                                                                                                 cGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTc                              <  2:603753/150‑1 (MQ=255)
                                                                                                                 cGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTc                              >  2:699782/1‑150 (MQ=255)
                                                                                                                 cGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATGTCCCTGAATAACTGTAGTGTTTTc                              <  2:124702/150‑1 (MQ=255)
                                                                                                                       gCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGgcg                        <  2:2843663/150‑1 (MQ=255)
                                                                                                                        cAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAANGGTGAAATANTTTCCCTGAATAACTGTAGTGTTTTCAGGgcgc                       >  1:315868/1‑150 (MQ=255)
                                                                                                                           gTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGc                    >  1:1707868/1‑150 (MQ=255)
                                                                                                                            ttAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCa                   >  2:2768025/1‑150 (MQ=255)
                                                                                                                             tAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCat                  >  2:2100007/1‑150 (MQ=255)
                                                                                                                              aaGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCata                 <  1:847162/150‑1 (MQ=255)
                                                                                                                               aGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCataa                <  2:3429871/150‑1 (MQ=255)
                                                                                                                                  aGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCAtaataa             >  1:3639323/1‑150 (MQ=255)
                                                                                                                                  aGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCAtaataa             >  2:3355297/1‑150 (MQ=255)
                                                                                                                                        gtggtCATGCCTAAGCCCCTCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGcc       <  2:3192917/150‑1 (MQ=255)
                                                                                                                                        gtggtCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGcc       <  1:3990737/150‑1 (MQ=255)
                                                                                                                                         tggtCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCa      >  2:58127/1‑150 (MQ=255)
                                                                                                                                          ggtCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAg     <  1:1424822/150‑1 (MQ=255)
                                                                                                                                          ggtCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAg     >  2:3620667/1‑150 (MQ=255)
                                                                                                                                            tCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTg   >  2:801683/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGG  >  CP009273/10297‑10587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑