Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,610,766 C→T 16.8% S249S (AGC→AGT)  yneK → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,610,7660CT16.8% 751.9 / 121.9 280S249S (AGC→AGT) yneKuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (109/123);  new base T (22/25);  total (131/149)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 280 total aligned reads displayed.

TTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATG  >  CP009273/1610619‑1610913
                                                                                                                                                   |                                                                                                                                                   
   tATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGAt                                                                                                                                                >  2:3927588/1‑150 (MQ=255)
   tATCTCTCGCGGTAATTTTGTCATACTCCCCCGAATATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGAt                                                                                                                                                >  2:4437026/1‑150 (MQ=255)
      ctctcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGa                                                                                                                                             <  1:1607656/150‑1 (MQ=255)
        ctcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGAtt                                                                                                                                           <  1:1958229/150‑1 (MQ=255)
         tcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTc                                                                                                                                          <  2:2338932/150‑1 (MQ=255)
         tcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTc                                                                                                                                          <  2:31416/150‑1 (MQ=255)
          cgcgGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCa                                                                                                                                         >  2:1104401/1‑150 (MQ=255)
               tAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAgg                                                                                                                                    >  1:3455280/1‑150 (MQ=255)
                aaTTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGt                                                                                                                                   >  1:2479368/1‑150 (MQ=255)
                  ttttGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTc                                                                                                                                 <  1:3967487/150‑1 (MQ=255)
                   tttGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCt                                                                                                                                <  1:3051203/150‑1 (MQ=255)
                       tCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTaaca                                                                                                                            >  2:2415727/1‑150 (MQ=255)
                         aTACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTaacaaa                                                                                                                          >  1:811107/1‑150 (MQ=255)
                           aCTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTaacaaaca                                                                                                                        >  2:3266209/1‑150 (MQ=255)
                             tCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATg                                                                                                                      <  2:847975/150‑1 (MQ=255)
                                 ccGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGtact                                                                                                                  >  1:2793945/1‑150 (MQ=255)
                                    aTTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATTAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTTAAAGAGGATTTGTTGATATGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTAcc                                                                                                               <  2:4000579/150‑1 (MQ=255)
                                    aTTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTAcc                                                                                                               >  1:1226625/1‑150 (MQ=255)
                                    aTTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTAcc                                                                                                               <  1:3391112/150‑1 (MQ=255)
                                     ttATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTAccc                                                                                                              <  1:1446606/150‑1 (MQ=255)
                                     ttATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACGAccc                                                                                                              <  1:4384855/150‑1 (MQ=255)
                                         ggAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAg                                                                                                          >  1:2631373/1‑150 (MQ=255)
                                         ggAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAg                                                                                                          >  1:1122608/1‑150 (MQ=255)
                                           aaTAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGAACTACCCGAAGTc                                                                                                        <  1:4044142/150‑1 (MQ=255)
                                            aTAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCt                                                                                                       <  2:1085239/150‑1 (MQ=255)
                                            aTAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATACTAACTTGATCACCGGCATTCTCGACATAACGTACAGCGAATTAAAAGAGGATTTGTTGCAAATCCTGATGGATTCATTAGGTTCTAACAAACATGTAATACCCGAAGTCt                                                                                                       <  1:4294008/150‑1 (MQ=255)
                                               aCTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCa                                                                                                    >  2:4322521/1‑150 (MQ=255)
                                                  ctGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCt                                                                                                 <  1:1355364/150‑1 (MQ=255)
                                                     aGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCt                                                                                              >  1:3219670/1‑150 (MQ=255)
                                                       gTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCttt                                                                                            <  2:2144809/150‑1 (MQ=255)
                                                       gTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCttt                                                                                            <  2:4103261/150‑1 (MQ=255)
                                                        ttCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCtttt                                                                                           >  1:3103405/1‑150 (MQ=255)
                                                        ttCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCtttt                                                                                           <  2:1589439/150‑1 (MQ=255)
                                                         tCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCttttt                                                                                          >  1:2383857/1‑150 (MQ=255)
                                                         tCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCttttt                                                                                          >  1:3258352/1‑150 (MQ=255)
                                                           acaGATGGTTCTTCAATAAAGATCCTAACTTGATCACCGCCATTCTCTACATAAAGGACAGCGAATTAATAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATTTACTACCCGAAGTCTGCATCTGCTTTTTAt                                                                                        <  2:4369337/150‑1 (MQ=255)
                                                             aGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATcc                                                                                      >  2:745553/1‑150 (MQ=255)
                                                              gATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATccc                                                                                     >  2:3130887/1‑150 (MQ=255)
                                                                 ggTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCttt                                                                                  <  2:2177011/150‑1 (MQ=255)
                                                                     cGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGc                                                                              >  1:4093537/1‑150 (MQ=255)
                                                                     cGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGc                                                                              <  2:814777/150‑1 (MQ=255)
                                                                     cGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGc                                                                              <  2:3123660/150‑1 (MQ=255)
                                                                      gTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCa                                                                             >  1:294723/1‑150 (MQ=255)
                                                                          aTAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAAc                                                                         <  1:3909511/150‑1 (MQ=255)
                                                                          aTAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAAc                                                                         <  1:300064/150‑1 (MQ=255)
                                                                             aaGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAg                                                                      <  2:176341/150‑1 (MQ=255)
                                                                              aGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGa                                                                     >  1:676168/1‑150 (MQ=255)
                                                                                  ccTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAAtct                                                                 >  1:3320441/1‑150 (MQ=255)
                                                                                    tAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAAAAAGAATctcc                                                               >  1:881830/1‑150 (MQ=255)
                                                                                     aaCTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcct                                                              >  1:2873887/1‑150 (MQ=255)
                                                                                     aaCTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcct                                                              >  1:2874376/1‑150 (MQ=255)
                                                                                      aCTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcctc                                                             >  2:336197/1‑150 (MQ=255)
                                                                                      aCTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcctc                                                             >  1:1790339/1‑150 (MQ=255)
                                                                                          gATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAttt                                                         <  2:3026341/150‑1 (MQ=255)
                                                                                           aTCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAtttt                                                        >  2:3767704/1‑150 (MQ=255)
                                                                                           aTCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAtttt                                                        <  1:3741720/150‑1 (MQ=255)
                                                                                              aCCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAg                                                     <  2:1979124/150‑1 (MQ=255)
                                                                                              aCCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCATTTTAGCAGAACAAGAATCTCCTCATTTTCAg                                                     <  2:1820164/150‑1 (MQ=255)
                                                                                               ccGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGa                                                    <  2:520286/150‑1 (MQ=255)
                                                                                                 gCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAAc                                                  >  2:905862/1‑150 (MQ=255)
                                                                                                 gCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAAc                                                  >  2:2403059/1‑150 (MQ=255)
                                                                                                   cATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGACTCTCCTCATTTTCAGAACtt                                                >  2:1563356/1‑150 (MQ=255)
                                                                                                    aTTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTg                                               <  1:785988/150‑1 (MQ=255)
                                                                                                    aTTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTg                                               >  1:2693409/1‑150 (MQ=255)
                                                                                                    aTTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTg                                               >  1:4450336/1‑150 (MQ=255)
                                                                                                     ttCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGt                                              >  1:2527757/1‑150 (MQ=255)
                                                                                                      tctcGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGtt                                             <  1:3350987/150‑1 (MQ=255)
                                                                                                      tctcGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGtt                                             >  2:723413/1‑150 (MQ=255)
                                                                                                       ctcGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttt                                            >  2:2712985/1‑150 (MQ=255)
                                                                                                       ctcGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttt                                            <  1:1316087/150‑1 (MQ=255)
                                                                                                        tcGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGtttt                                           <  2:2606070/150‑1 (MQ=255)
                                                                                                          gACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTa                                         <  2:610939/150‑1 (MQ=255)
                                                                                                          gACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTa                                         <  2:2051058/150‑1 (MQ=255)
                                                                                                          gACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTa                                         <  1:1012201/150‑1 (MQ=255)
                                                                                                           aCATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAt                                        >  1:2049502/1‑150 (MQ=255)
                                                                                                            cATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAtt                                       <  2:4448480/150‑1 (MQ=255)
                                                                                                            cATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAtt                                       >  1:696898/1‑150 (MQ=255)
                                                                                                            cATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAtt                                       <  2:391024/150‑1 (MQ=255)
                                                                                                             aTAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAttt                                      >  1:947431/1‑150 (MQ=255)
                                                                                                             aTAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAttt                                      <  2:3711857/150‑1 (MQ=255)
                                                                                                                aCGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTc                                   >  1:505525/1‑150 (MQ=255)
                                                                                                                   gACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGcc                                >  1:1252346/1‑150 (MQ=255)
                                                                                                                    aCAGAGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCa                               <  1:1864162/150‑1 (MQ=255)
                                                                                                                         gAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATg                          >  2:2927434/1‑150 (MQ=255)
                                                                                                                          aaTTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGt                         <  2:2813763/150‑1 (MQ=255)
                                                                                                                          aaTTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGt                         <  2:2747126/150‑1 (MQ=255)
                                                                                                                          aaTTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGt                         <  2:4409429/150‑1 (MQ=255)
                                                                                                                          aaTTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGt                         >  1:2144888/1‑150 (MQ=255)
                                                                                                                          aaTTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGt                         <  1:3596728/150‑1 (MQ=255)
                                                                                                                           aTTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGtt                        <  2:1254527/150‑1 (MQ=255)
                                                                                                                            ttAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACCAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTa                       <  2:2973041/150‑1 (MQ=255)
                                                                                                                            ttAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTa                       <  2:1214932/150‑1 (MQ=255)
                                                                                                                                 agagGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCa                  <  2:3550081/150‑1 (MQ=255)
                                                                                                                                   agGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGctct                >  2:4265308/1‑150 (MQ=255)
                                                                                                                                    ggATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTa               <  2:1954311/150‑1 (MQ=255)
                                                                                                                                       tttGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCa            >  2:1675514/1‑150 (MQ=255)
                                                                                                                                        ttgttgCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCAc           <  2:2532495/150‑1 (MQ=255)
                                                                                                                                         tgttgCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCAcc          <  1:3673013/150‑1 (MQ=255)
                                                                                                                                               aaaGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTa    >  1:184832/1‑150 (MQ=255)
                                                                                                                                                aaGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTAt   >  1:757830/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATG  >  CP009273/1610619‑1610913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑