BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,616,682	T→G	10.5%	L365R (CTG→CGG)	ydeE →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,616,682	0	T	G	10.5%	708.1 / 84.9	268	L365R (CTG→CGG)	ydeE	putative transporter
Reads supporting (aligned to +/- strand): ref base T (127/113); new base G (17/11); total (144/124)
Fisher's exact test for biased strand distribution p-value = 5.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.57e-01

Only 100 of 273 total aligned reads displayed.

TGCGCCGGGCGAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACCTCTGCT  >  CP009273/1616533‑1616831

      gggCGAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTACCTCGCTGTTTGt                                                                                                                                                 >  2:3003452/1‑150 (MQ=255)

        gCGAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCa                                                                                                                                               >  2:2417178/1‑150 (MQ=255)

        gCGAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCa                                                                                                                                               >  1:2492681/1‑150 (MQ=255)

          gAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATc                                                                                                                                             >  1:1518251/1‑150 (MQ=255)

           aGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCt                                                                                                                                            >  2:808447/1‑150 (MQ=255)

              atatGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAg                                                                                                                                         >  1:2430856/1‑150 (MQ=255)

              atatGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAg                                                                                                                                         <  2:4585972/150‑1 (MQ=255)

                 tGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGt                                                                                                                                      >  1:1443850/1‑150 (MQ=255)

                   ttgattgaCCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTg                                                                                                                                    >  1:2412205/1‑150 (MQ=255)

                    tgattgaCCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTgg                                                                                                                                   >  2:3086661/1‑150 (MQ=255)

                         gaCCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATc                                                                                                                              <  2:2548743/150‑1 (MQ=255)

                           ccATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCAt                                                                                                                            <  1:897133/150‑1 (MQ=255)

                              tatTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATtgc                                                                                                                         >  2:641249/1‑150 (MQ=255)

                               atTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATtgct                                                                                                                        <  1:1660706/150‑1 (MQ=255)

                                   cgccgcCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGt                                                                                                                    >  1:1257407/1‑150 (MQ=255)

                                    gccgccAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTg                                                                                                                   >  2:1454183/1‑150 (MQ=255)

                                       gccAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGt                                                                                                                <  1:2768235/150‑1 (MQ=255)

                                        ccAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTg                                                                                                               >  2:603062/1‑150 (MQ=255)

                                        ccAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTg                                                                                                               >  1:1330657/1‑150 (MQ=255)

                                        ccAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTg                                                                                                               >  1:2537122/1‑150 (MQ=255)

                                          aGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCt                                                                                                             >  1:1572807/1‑150 (MQ=255)

                                           gAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTg                                                                                                            <  2:169836/150‑1 (MQ=255)

                                           gAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTg                                                                                                            >  1:3256810/1‑150 (MQ=255)

                                             aaTGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGAt                                                                                                          <  1:1084309/150‑1 (MQ=255)

                                              aTGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATg                                                                                                         >  2:4042964/1‑150 (MQ=255)

                                               tGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGt                                                                                                        >  1:3538229/1‑150 (MQ=255)

                                               tGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGt                                                                                                        >  1:1219059/1‑150 (MQ=255)

                                                 aaaGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTa                                                                                                      <  1:709887/150‑1 (MQ=255)

                                                  aaGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTaa                                                                                                     >  1:2019146/1‑150 (MQ=255)

                                                  aaGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTaa                                                                                                     <  2:2821513/150‑1 (MQ=255)

                                                      cAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAgg                                                                                                 >  1:1838626/1‑150 (MQ=255)

                                                        gCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGa                                                                                               <  2:82587/150‑1 (MQ=255)

                                                           aTTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTc                                                                                            >  1:2150218/1‑150 (MQ=255)

                                                            tttttCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCTTCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCg                                                                                           <  2:4591425/150‑1 (MQ=255)

                                                            tttttCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCg                                                                                           <  1:1822139/150‑1 (MQ=255)

                                                              tttCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAg                                                                                         >  1:549557/1‑150 (MQ=255)

                                                               ttCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGc                                                                                        <  1:3375871/150‑1 (MQ=255)

                                                                tCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCa                                                                                       >  1:3590097/1‑150 (MQ=255)

                                                                    cccAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGAc                                                                                   >  2:4478370/1‑150 (MQ=255)

                                                                    cccAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGAc                                                                                   >  2:1344128/1‑150 (MQ=255)

                                                                         tCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTgg                                                                              <  1:1959831/150‑1 (MQ=255)

                                                                            ttAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTggggg                                                                           >  1:686391/1‑150 (MQ=255)

                                                                             tAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGc                                                                          >  2:227528/1‑150 (MQ=255)

                                                                                gctggctTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGc                                                                       >  1:1912278/1‑150 (MQ=255)

                                                                                 ctggctTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGcc                                                                      >  1:1126455/1‑150 (MQ=255)

                                                                                 ctggctTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGcc                                                                      >  1:1290481/1‑150 (MQ=255)

                                                                                 ctggctTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGcc                                                                      >  1:2163006/1‑150 (MQ=255)

                                                                                  tggctTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGccc                                                                     <  2:2596831/150‑1 (MQ=255)

                                                                                      ttGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCAGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGGTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCgcgc                                                                 >  1:1061324/1‑150 (MQ=255)

                                                                                       tGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCt                                                                >  1:1395471/1‑150 (MQ=255)

                                                                                        ggTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCtt                                                               <  1:3190028/150‑1 (MQ=255)

                                                                                         gTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCttt                                                              <  2:1254013/150‑1 (MQ=255)

                                                                                          tGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTttg                                                             >  1:1046069/1‑150 (MQ=255)

                                                                                          tGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTttg                                                             <  1:1673486/150‑1 (MQ=255)

                                                                                          tGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTttg                                                             <  1:1596552/150‑1 (MQ=255)

                                                                                            ccGCGATTAACCCATGAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTttgtt                                                           <  1:4106053/150‑1 (MQ=255)

                                                                                              gcgATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGa                                                         >  2:3515791/1‑150 (MQ=255)

                                                                                                gATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGAtt                                                       >  2:2628444/1‑150 (MQ=255)

                                                                                                gATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGAtt                                                       <  2:1117461/150‑1 (MQ=255)

                                                                                                 aTTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGAttt                                                      <  1:621801/150‑1 (MQ=255)

                                                                                                 aTTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGAttt                                                      >  2:1942944/1‑150 (MQ=255)

                                                                                                 aTTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGAttt                                                      >  2:1651877/1‑150 (MQ=255)

                                                                                                 aTTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGAttt                                                      <  2:2511936/150‑1 (MQ=255)

                                                                                                    aaCCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAg                                                   >  1:2615166/1‑150 (MQ=255)

                                                                                                     aCCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGt                                                  >  1:1886317/1‑150 (MQ=255)

                                                                                                      cccATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTc                                                 <  2:2481377/150‑1 (MQ=255)

                                                                                                        cATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGa                                               >  2:487853/1‑150 (MQ=255)

                                                                                                         aTTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGaa                                              >  2:1018012/1‑150 (MQ=255)

                                                                                                         aTTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGaa                                              <  2:587993/150‑1 (MQ=255)

                                                                                                           tAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAaca                                            >  2:3249967/1‑150 (MQ=255)

                                                                                                           tAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAaca                                            <  2:3203395/150‑1 (MQ=255)

                                                                                                               gagtgGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAAt                                        <  1:4259559/150‑1 (MQ=255)

                                                                                                                 gtgGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATaa                                      <  1:1748234/150‑1 (MQ=255)

                                                                                                                  tgGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATaaa                                     >  2:724675/1‑150 (MQ=255)

                                                                                                                  tgGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATaaa                                     >  1:3150178/1‑150 (MQ=255)

                                                                                                                  tgGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATaaa                                     >  1:4167830/1‑150 (MQ=255)

                                                                                                                    gCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGa                                   <  1:1454201/150‑1 (MQ=255)

                                                                                                                     cGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGAt                                  <  2:4277581/150‑1 (MQ=255)

                                                                                                                      gTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGAtt                                 <  1:469795/150‑1 (MQ=255)

                                                                                                                       tAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGAttt                                <  1:491239/150‑1 (MQ=255)

                                                                                                                        aGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTa                               >  2:2388279/1‑150 (MQ=255)

                                                                                                                          tGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAAt                             <  2:155242/150‑1 (MQ=255)

                                                                                                                            cTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTc                           <  2:292816/150‑1 (MQ=255)

                                                                                                                             tAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCa                          >  1:3018538/1‑150 (MQ=255)

                                                                                                                                  aGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCtt                     >  1:3074125/1‑150 (MQ=255)

                                                                                                                                      tGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGtt                 <  1:4439226/150‑1 (MQ=255)

                                                                                                                                       gccgccTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGttt                >  1:2074685/1‑150 (MQ=255)

                                                                                                                                        ccgccTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTa               <  1:3650682/150‑1 (MQ=255)

                                                                                                                                        ccgccTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTa               >  1:2911177/1‑150 (MQ=255)

                                                                                                                                          gccTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAgg             <  2:11580/150‑1 (MQ=255)

                                                                                                                                            cTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGtt           <  2:3451425/150‑1 (MQ=255)

                                                                                                                                             ttCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTa          >  1:635607/1‑150 (MQ=255)

                                                                                                                                             ttCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTa          >  2:376083/1‑150 (MQ=255)

                                                                                                                                              tCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTAc         <  2:440035/150‑1 (MQ=255)

                                                                                                                                                cTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACct       >  1:2355491/1‑150 (MQ=255)

                                                                                                                                                 tCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACctc      <  1:4088641/150‑1 (MQ=255)

                                                                                                                                                  cGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACctct     <  2:3715842/150‑1 (MQ=255)

                                                                                                                                                    cTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACCTCTGc   <  1:808447/150‑1 (MQ=255)

                                                                                                                                                    cTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACCTCTGc   >  1:879932/1‑150 (MQ=255)

                                                                                                                                                     tGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACCTCTGCt  <  2:1050023/150‑1 (MQ=255)

TGCGCCGGGCGAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACCTCTGCT  >  CP009273/1616533‑1616831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`