Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,648,200 G→T 11.3% T400K (ACG→AAG)  rspA ← bifunctional D‑altronate/D‑mannonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,648,2000GT11.3% 916.0 / 59.6 255T400K (ACG→AAG) rspAbifunctional D‑altronate/D‑mannonate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (115/111);  new base T (13/16);  total (128/127)
Fisher's exact test for biased strand distribution p-value = 5.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 259 total aligned reads displayed.

CTATCTGAACCACAAATTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTCCAGTTGTGCGGGAAGACTTCG  >  CP009273/1648051‑1648349
                                                                                                                                                     |                                                                                                                                                     
cTATCTGAACCACAAATTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCg                                                                                                                                                       >  2:3922458/1‑150 (MQ=255)
 tATCTGAACCACAAATTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGt                                                                                                                                                      <  1:485215/150‑1 (MQ=255)
  aTCTGAACCACAAATTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTg                                                                                                                                                     <  1:91327/150‑1 (MQ=255)
      gAACCACAAATTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCAt                                                                                                                                                 >  2:4280606/1‑150 (MQ=255)
      gAACCACAAATTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCAt                                                                                                                                                 <  1:3781010/150‑1 (MQ=255)
       aaCCACAAATTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATc                                                                                                                                                >  1:3559524/1‑150 (MQ=255)
        aCCACAAATTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACACTCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCt                                                                                                                                               >  2:1603098/1‑150 (MQ=255)
        aCCACAAATTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCt                                                                                                                                               <  2:3117194/150‑1 (MQ=255)
         ccACAAATTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCtt                                                                                                                                              <  2:3528792/150‑1 (MQ=255)
             aaaTTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAg                                                                                                                                          <  1:2954083/150‑1 (MQ=255)
             aaaTTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAg                                                                                                                                          >  1:450132/1‑150 (MQ=255)
             aaaTTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAg                                                                                                                                          <  1:2954044/150‑1 (MQ=255)
             aaaTTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAg                                                                                                                                          >  1:3263712/1‑150 (MQ=255)
             aaaTTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAg                                                                                                                                          <  2:2111512/150‑1 (MQ=255)
              aaTTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGa                                                                                                                                         >  1:4591839/1‑150 (MQ=255)
                   cGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGc                                                                                                                                    <  1:2841183/150‑1 (MQ=255)
                    ggCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCTTGCCATCTTCCAGACGTGCg                                                                                                                                   <  2:889954/150‑1 (MQ=255)
                          tttCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGt                                                                                                                             <  1:644896/150‑1 (MQ=255)
                           ttCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCTTGCCATCTTCCAGACGTGCGACTGGTa                                                                                                                            <  1:155877/150‑1 (MQ=255)
                                 tttACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCTTGCCATCTTCCAGACGTGCGACTGGTAGATAAg                                                                                                                      <  1:4161554/150‑1 (MQ=255)
                                    aCTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAg                                                                                                                   >  2:1043124/1‑150 (MQ=255)
                                    aCTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAg                                                                                                                   <  1:3294549/150‑1 (MQ=255)
                                     cTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAgg                                                                                                                  >  2:4527380/1‑150 (MQ=255)
                                      tCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGt                                                                                                                 >  2:2422176/1‑150 (MQ=255)
                                       cGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGtt                                                                                                                >  2:2779332/1‑150 (MQ=255)
                                        gTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACACTCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTc                                                                                                               <  2:940026/150‑1 (MQ=255)
                                        gTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTc                                                                                                               >  2:3427712/1‑150 (MQ=255)
                                          aCTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCAt                                                                                                             >  2:3008209/1‑150 (MQ=255)
                                          aCTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCAt                                                                                                             >  2:1397231/1‑150 (MQ=255)
                                          aCTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCAt                                                                                                             >  2:2536184/1‑150 (MQ=255)
                                          aCTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCAt                                                                                                             <  1:2360416/150‑1 (MQ=255)
                                            ttCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCTTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAg                                                                                                           >  2:4072910/1‑150 (MQ=255)
                                             tCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAgg                                                                                                          <  1:1335655/150‑1 (MQ=255)
                                              cACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAggg                                                                                                         >  1:2067652/1‑150 (MQ=255)
                                                cccGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGat                                                                                                       >  2:78758/1‑150 (MQ=255)
                                                 ccGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCTTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGata                                                                                                      <  2:4477772/150‑1 (MQ=255)
                                                 ccGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGata                                                                                                      <  2:3529539/150‑1 (MQ=255)
                                                  cGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGatat                                                                                                     <  2:4287797/150‑1 (MQ=255)
                                                  cGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGatat                                                                                                     <  2:847529/150‑1 (MQ=255)
                                                    cTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCTTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATAttt                                                                                                   <  2:4225908/150‑1 (MQ=255)
                                                            gtgggtATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCa                                                                                           <  1:4356457/150‑1 (MQ=255)
                                                                 tATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCttt                                                                                      <  2:4049149/150‑1 (MQ=255)
                                                                 tATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCttt                                                                                      <  2:3282328/150‑1 (MQ=255)
                                                                  aTTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCtttt                                                                                     <  2:3677109/150‑1 (MQ=255)
                                                                    ttCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCa                                                                                   <  1:3344880/150‑1 (MQ=255)
                                                                     tCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCAt                                                                                  <  2:1316958/150‑1 (MQ=255)
                                                                        cGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGa                                                                               >  2:4627369/1‑150 (MQ=255)
                                                                            cGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTc                                                                           >  2:3071607/1‑150 (MQ=255)
                                                                            cGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTc                                                                           <  1:555975/150‑1 (MQ=255)
                                                                            cGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTc                                                                           <  2:2535002/150‑1 (MQ=255)
                                                                             gACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCg                                                                          <  1:1653338/150‑1 (MQ=255)
                                                                               cAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGAt                                                                        <  1:843867/150‑1 (MQ=255)
                                                                               cAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGAt                                                                        <  1:4624252/150‑1 (MQ=255)
                                                                                aaTCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATa                                                                       >  1:2121679/1‑150 (MQ=255)
                                                                                aaTCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATa                                                                       >  1:2211424/1‑150 (MQ=255)
                                                                                aaTCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATa                                                                       <  1:24957/150‑1 (MQ=255)
                                                                                 aTCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATAc                                                                      <  2:2090840/150‑1 (MQ=255)
                                                                                   cGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCa                                                                    >  2:1988235/1‑150 (MQ=255)
                                                                                     ccAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCTTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAg                                                                  >  2:2056482/1‑150 (MQ=255)
                                                                                     ccAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAg                                                                  <  2:3102993/150‑1 (MQ=255)
                                                                                      cAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGa                                                                 <  1:1276892/150‑1 (MQ=255)
                                                                                       aGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGAc                                                                >  2:3800749/1‑150 (MQ=255)
                                                                                       aGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGAc                                                                <  1:1106089/150‑1 (MQ=255)
                                                                                          tGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCg                                                             >  1:4338259/1‑150 (MQ=255)
                                                                                          tGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCg                                                             <  1:688659/150‑1 (MQ=255)
                                                                                           gATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCTTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCgg                                                            <  1:772533/150‑1 (MQ=255)
                                                                                           gATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCgg                                                            >  2:1080597/1‑150 (MQ=255)
                                                                                              tCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGttt                                                         <  2:1719819/150‑1 (MQ=255)
                                                                                              tCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGttt                                                         >  1:2683006/1‑150 (MQ=255)
                                                                                              tCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGttt                                                         <  2:3215169/150‑1 (MQ=255)
                                                                                              tCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGttt                                                         <  1:3162089/150‑1 (MQ=255)
                                                                                                ggTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGt                                                       <  2:569788/150‑1 (MQ=255)
                                                                                                     ttCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTccc                                                  >  2:4409923/1‑150 (MQ=255)
                                                                                                      tCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCg                                                 <  2:1587029/150‑1 (MQ=255)
                                                                                                      tCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCg                                                 <  1:2538212/150‑1 (MQ=255)
                                                                                                           tAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGc                                            >  1:115932/1‑150 (MQ=255)
                                                                                                             atatGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCat                                          >  1:793456/1‑150 (MQ=255)
                                                                                                                        aTTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTAt                               <  2:3739981/150‑1 (MQ=255)
                                                                                                                             cTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGaaa                          >  1:4428756/1‑150 (MQ=255)
                                                                                                                              ttACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAg                         >  1:2739130/1‑150 (MQ=255)
                                                                                                                              ttACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAg                         >  2:3666503/1‑150 (MQ=255)
                                                                                                                              ttACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAg                         >  2:190335/1‑150 (MQ=255)
                                                                                                                                aCTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTc                       >  2:2895170/1‑150 (MQ=255)
                                                                                                                                 cTCCTTACCAGTTCCACAGCTTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTcc                      <  2:3025695/150‑1 (MQ=255)
                                                                                                                                 cTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTcc                      <  2:2272110/150‑1 (MQ=255)
                                                                                                                                     ttACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTCCAGtt                  >  1:1408461/1‑150 (MQ=255)
                                                                                                                                      tACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGAGACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTCCAGTtg                 <  2:668745/150‑1 (MQ=255)
                                                                                                                                        ccAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTCCAGTtgtg               >  2:1831322/1‑150 (MQ=255)
                                                                                                                                        ccAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTCCAGTtgtg               >  1:1366508/1‑150 (MQ=255)
                                                                                                                                          aGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTCCAGTTGTGCg             >  2:3263368/1‑150 (MQ=255)
                                                                                                                                               cacaGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTCCAGTTGTGCGGGAAg        >  2:1259772/1‑150 (MQ=255)
                                                                                                                                                acaGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTCCAGTTGTGCGGGAAGa       >  1:3912561/1‑150 (MQ=255)
                                                                                                                                                  aGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTCCAGTTGTGCGGGAAGACt     >  2:2477660/1‑150 (MQ=255)
                                                                                                                                                  aGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTCCAGTTGTGCGGGAAGACt     <  1:3092635/150‑1 (MQ=255)
                                                                                                                                                  aGCGTGCCAACTTCCAGACGTGCGACGGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCTGATGCATATAGCCGTTATCGAAAGTCCAGTTGTGCTGGAAGACt     <  2:1207230/150‑1 (MQ=255)
                                                                                                                                                    cGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATCTAGCCGTTATCGAAAGTCCAGTTGTGCGGGAAGACTTc   <  1:4314020/150‑1 (MQ=255)
                                                                                                                                                    cGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTCCAGTTGTGCGGGAAGACTTc   <  2:115628/150‑1 (MQ=255)
                                                                                                                                                    cGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTCCAGTTGTGCGGGAAGACTTc   <  1:4273186/150‑1 (MQ=255)
                                                                                                                                                    cGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTCCAGTTGTGCGGGAAGACTTc   <  1:2394865/150‑1 (MQ=255)
                                                                                                                                                    cGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTCCAGTTGTGCGGGAAGACTTc   <  1:166709/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTATCTGAACCACAAATTCCGGCAAGTTTCACTTTTACTCGTACTTCACCCGCTGACGGGGTGGGTATTTCACGTTCGACAATCGCCAGTTGATTCGGTTTTTCAATTAATATGCTTTTCATTATCTTACTCCTTACCAGTTCCACAGCGTGCCATCTTCCAGACGTGCGACTGGTAGATAAGCAGGTTCATAGGGATATTTCGCCGCCAGCTTTTCATCGAATTCGATACCAAGACCCGGTTTGTCTCCCGGATGCATATAGCCGTTATCGAAAGTCCAGTTGTGCGGGAAGACTTCG  >  CP009273/1648051‑1648349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑