BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,697,123	C→A	10.3%	R212S (CGT→AGT)	add →	adenosine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,697,123	0	C	A	10.3%	1038.3 / 59.6	289	R212S (CGT→AGT)	add	adenosine deaminase
Reads supporting (aligned to +/- strand): ref base C (108/150); new base A (11/19); total (119/170)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

AGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAGCTGGCTGCACAT  >  CP009273/1696974‑1697272

aGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTc                                                                                                                                                       <  1:3845051/150‑1 (MQ=255)

  aTTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTAGt                                                                                                                                                     <  1:3548018/150‑1 (MQ=255)

  aTTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGAGGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGt                                                                                                                                                     <  1:2090139/150‑1 (MQ=255)

      ccGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTAGTGAAc                                                                                                                                                 >  1:4100495/1‑150 (MQ=255)

       cGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACt                                                                                                                                                >  1:292995/1‑150 (MQ=255)

       cGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTAGTGAACt                                                                                                                                                >  1:58717/1‑150 (MQ=255)

         cACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTgg                                                                                                                                              <  2:1611429/150‑1 (MQ=255)

           cTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGt                                                                                                                                            <  2:4305761/150‑1 (MQ=255)

             tGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGc                                                                                                                                          >  2:3823697/1‑150 (MQ=255)

                  tAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGc                                                                                                                                     >  1:312280/1‑150 (MQ=255)

                   aGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCg                                                                                                                                    >  2:4095253/1‑150 (MQ=255)

                   aGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCg                                                                                                                                    >  1:3478257/1‑150 (MQ=255)

                   aGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCg                                                                                                                                    >  2:91604/1‑150 (MQ=255)

                    gCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGt                                                                                                                                   <  2:1947251/150‑1 (MQ=255)

                     ccGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTa                                                                                                                                  >  2:984173/1‑150 (MQ=255)

                      cGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTAt                                                                                                                                 >  1:1208417/1‑150 (MQ=255)

                      cGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTAt                                                                                                                                 >  2:2126015/1‑150 (MQ=255)

                      cGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTAt                                                                                                                                 <  1:1004457/150‑1 (MQ=255)

                        gtgtTGAACTTGGTTTCCCGTGTAGTCTGTTCGTTTCTCACTTCTATCGGGCGCGTGATGCGGGATGGCATATTACCGTCCATGCAGGCGATGGTGCCGGGCCGGATAGCTTGTGGAATGCGATTCGTTAACTGTGTGCGGATCGTTTTg                                                                                                                               <  2:3387775/150‑1 (MQ=255)

                          gatgaACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGa                                                                                                                             >  2:4479646/1‑150 (MQ=255)

                           atgaACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGAc                                                                                                                            >  2:546899/1‑150 (MQ=255)

                            tgaACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACa                                                                                                                           <  1:326762/150‑1 (MQ=255)

                             gaACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGTCGATTCGTGAACTGGGTGCGGAGCGTATTGGACAt                                                                                                                          <  1:3703779/150‑1 (MQ=255)

                             gaACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACAt                                                                                                                          >  2:3190548/1‑150 (MQ=255)

                              aaCTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATg                                                                                                                         <  1:3510369/150‑1 (MQ=255)

                               aCTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATgg                                                                                                                        <  2:2361423/150‑1 (MQ=255)

                                 ttGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCg                                                                                                                      >  2:4452803/1‑150 (MQ=255)

                                   ggTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTa                                                                                                                    <  1:2270737/150‑1 (MQ=255)

                                    gtgtCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTaa                                                                                                                   <  2:2913718/147‑1 (MQ=255)

                                       tCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAg                                                                                                                <  1:631143/150‑1 (MQ=255)

                                          cGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCa                                                                                                             <  1:1476550/150‑1 (MQ=255)

                                           gggAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCAt                                                                                                            >  2:1343501/1‑150 (MQ=255)

                                            ggAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCAtt                                                                                                           >  1:108665/1‑150 (MQ=255)

                                            ggAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCAtt                                                                                                           >  1:2707167/1‑150 (MQ=255)

                                             gAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTg                                                                                                          >  2:1491086/1‑150 (MQ=255)

                                             gAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTg                                                                                                          >  1:2689583/1‑150 (MQ=255)

                                             gAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTg                                                                                                          >  1:901009/1‑150 (MQ=255)

                                                gTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAg                                                                                                       <  1:3372154/150‑1 (MQ=255)

                                                gTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAg                                                                                                       >  2:2049780/1‑150 (MQ=255)

                                                gTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAg                                                                                                       >  1:4532215/1‑150 (MQ=255)

                                                 tCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGa                                                                                                      <  1:4390016/150‑1 (MQ=255)

                                                  cTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGAt                                                                                                     <  2:3503100/150‑1 (MQ=255)

                                                  cTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGAt                                                                                                     <  1:2041454/150‑1 (MQ=255)

                                                   tGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATc                                                                                                    >  2:4176322/1‑150 (MQ=255)

                                                   tGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATc                                                                                                    <  2:1117232/150‑1 (MQ=255)

                                                    gTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCg                                                                                                   <  1:2371600/150‑1 (MQ=255)

                                                        cTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCTGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGgcg                                                                                               <  1:1364749/150‑1 (MQ=255)

                                                        cTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGgcg                                                                                               >  1:3027597/1‑150 (MQ=255)

                                                         tttCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGgcgc                                                                                              >  1:2004874/1‑150 (MQ=255)

                                                         tttCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGgcgc                                                                                              >  1:3058824/1‑150 (MQ=255)

                                                          ttCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCt                                                                                             >  2:2872006/1‑150 (MQ=255)

                                                              ctcTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATg                                                                                         <  2:3917028/148‑1 (MQ=255)

                                                              cACTTCACCCGCGCGCGTGCTGCGGGCGGGCATATTACCTTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCAATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATg                                                                                         <  2:2454614/150‑1 (MQ=255)

                                                                cTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGa                                                                                       >  1:2607384/1‑150 (MQ=255)

                                                                     aCCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTtc                                                                                  >  2:2245821/1‑150 (MQ=255)

                                                                      ccGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTtct                                                                                 >  2:3364188/1‑150 (MQ=255)

                                                                      ccGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTtct                                                                                 >  2:34178/1‑150 (MQ=255)

                                                                       cgcgcgcgTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGTTCCTGAAAGCATCTGTCAGGCGATTAGTGAACTTGTTGCGGAGCGTATTGGACATGGCGTAATAGCCATTGAAGATCGGTCGCTGATGGATTTtctc                                                                                <  2:2338179/150‑1 (MQ=255)

                                                                             cgTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAg                                                                          >  1:164124/1‑150 (MQ=255)

                                                                              gTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGc                                                                         <  2:3088724/150‑1 (MQ=255)

                                                                              gTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGc                                                                         <  2:3088708/150‑1 (MQ=255)

                                                                               tGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGca                                                                        <  1:737838/150‑1 (MQ=255)

                                                                                 aTGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGcaac                                                                      >  2:4196937/1‑150 (MQ=255)

                                                                                   gCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGcaacaa                                                                    >  1:1834515/1‑150 (MQ=255)

                                                                                     gggCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAAt                                                                  >  1:1582429/1‑150 (MQ=255)

                                                                                     gggCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAAt                                                                  >  2:1254882/1‑150 (MQ=255)

                                                                                     gggCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAAt                                                                  >  2:118217/1‑150 (MQ=255)

                                                                                      ggCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAAtt                                                                 >  1:1479867/1‑150 (MQ=255)

                                                                                      ggCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAAtt                                                                 <  1:2969192/150‑1 (MQ=255)

                                                                                        cTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGGAGGCGATTGGTGAACTGGGTGCGGAGCGTATTTGACATGGCGTAAAAGCCATTGAAGATCTGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTgg                                                               <  2:1371012/150‑1 (MQ=255)

                                                                                          ggCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTa                                                             >  2:1153986/1‑150 (MQ=255)

                                                                                             atatTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTg                                                          >  2:743770/1‑150 (MQ=255)

                                                                                                ttACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAAt                                                       <  1:4480718/150‑1 (MQ=255)

                                                                                                ttACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAAt                                                       <  1:489650/150‑1 (MQ=255)

                                                                                                 tACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATc                                                      >  2:1905968/1‑150 (MQ=255)

                                                                                                   ccGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCt                                                    >  2:4033425/1‑150 (MQ=255)

                                                                                                        cATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTg                                               <  1:2132982/150‑1 (MQ=255)

                                                                                                            cAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCt                                           <  2:1984598/150‑1 (MQ=255)

                                                                                                            cAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCt                                           <  2:885652/150‑1 (MQ=255)

                                                                                                              ggCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTcc                                         <  2:3102371/150‑1 (MQ=255)

                                                                                                               gCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTAGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCa                                        <  2:454185/150‑1 (MQ=255)

                                                                                                                cGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTAGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCaa                                       <  1:3237555/150‑1 (MQ=255)

                                                                                                                 gAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTAGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAat                                      <  2:3800539/150‑1 (MQ=255)

                                                                                                                  aaGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAata                                     <  1:3063553/150‑1 (MQ=255)

                                                                                                                        ccgggccggAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGa                               >  2:2169080/1‑150 (MQ=255)

                                                                                                                            gccggAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAg                           <  1:1505979/150‑1 (MQ=255)

                                                                                                                             ccggAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGc                          <  1:2880838/150‑1 (MQ=255)

                                                                                                                               ggAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCAc                        >  2:4115102/1‑150 (MQ=255)

                                                                                                                                  aaGCATCTGGCAGGCGATTAGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGt                     >  1:3994846/1‑150 (MQ=255)

                                                                                                                                   aGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTa                    <  1:2058772/150‑1 (MQ=255)

                                                                                                                                     cATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGc                  <  1:243438/150‑1 (MQ=255)

                                                                                                                                      aTCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCa                 >  1:2582970/1‑150 (MQ=255)

                                                                                                                                       tCTGGCAGGCGATTAGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCag                <  1:3101396/150‑1 (MQ=255)

                                                                                                                                           gCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAgct            >  2:2720874/1‑150 (MQ=255)

                                                                                                                                            cAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAgctg           <  2:2030402/150‑1 (MQ=255)

                                                                                                                                            cAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAgctg           <  2:4357710/150‑1 (MQ=255)

                                                                                                                                            cAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAgctg           <  2:1572315/150‑1 (MQ=255)

                                                                                                                                              ggCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAgctggc         <  1:1092154/150‑1 (MQ=255)

                                                                                                                                                    tCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAGCTGGCTGcaca   <  2:4625775/150‑1 (MQ=255)

                                                                                                                                                     cGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAGCTGGCTGCACAt  >  2:3685777/1‑150 (MQ=255)

AGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAGCTGGCTGCACAT  >  CP009273/1696974‑1697272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`