Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,821,696 A→T 90.7% L274Q (CTG→CAG)  astB ← succinylarginine dihydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,821,6960AT90.7% 825.0 / 58.7 257L274Q (CTG→CAG) astBsuccinylarginine dihydrolase
Reads supporting (aligned to +/- strand):  ref base A (17/7);  new base T (102/131);  total (119/138)
Fisher's exact test for biased strand distribution p-value = 1.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 257 total aligned reads displayed.

TGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGATT  >  CP009273/1821549‑1821844
                                                                                                                                                   |                                                                                                                                                    
   ggCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAc                                                                                                                                                 >  1:2640649/1‑150 (MQ=255)
     cagcaCCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACtg                                                                                                                                               <  1:3382949/150‑1 (MQ=255)
      agcaCCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACtgt                                                                                                                                              >  2:2460710/1‑150 (MQ=255)
       gcaCCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACtgtg                                                                                                                                             <  2:210283/150‑1 (MQ=255)
        caCCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGa                                                                                                                                            >  1:3888242/1‑150 (MQ=255)
        caCCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGa                                                                                                                                            <  2:4310927/150‑1 (MQ=255)
         aCCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGAc                                                                                                                                           <  1:920650/150‑1 (MQ=255)
               atcatGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGgcga                                                                                                                                     <  2:4282383/150‑1 (MQ=255)
                tcatGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGgcgag                                                                                                                                    >  1:3866636/1‑150 (MQ=255)
                  atGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTTACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGgcgagcg                                                                                                                                  >  1:3532635/1‑150 (MQ=255)
                    ggAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGaa                                                                                                                                >  1:3552037/1‑150 (MQ=255)
                      aaCCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACg                                                                                                                              >  2:1974103/1‑150 (MQ=255)
                       aCCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGc                                                                                                                             >  1:4477600/1‑150 (MQ=255)
                        ccATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGcc                                                                                                                            <  1:3160553/150‑1 (MQ=255)
                           tcatcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGt                                                                                                                         >  1:2796609/1‑150 (MQ=255)
                           tcatcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGt                                                                                                                         >  2:4394322/1‑150 (MQ=255)
                           tcatcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGt                                                                                                                         >  1:2890071/1‑150 (MQ=255)
                            catcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGtt                                                                                                                        <  1:2274967/150‑1 (MQ=255)
                                gcgGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTtggtg                                                                                                                    >  1:1110498/1‑150 (MQ=255)
                                gcgGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTtggtg                                                                                                                    <  2:800894/150‑1 (MQ=255)
                                  ggCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGc                                                                                                                  >  2:4583412/1‑150 (MQ=255)
                                       agcagTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAAc                                                                                                             >  1:1907259/1‑150 (MQ=255)
                                        gcagTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACa                                                                                                            <  2:2857257/150‑1 (MQ=255)
                                        gcagTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACa                                                                                                            <  2:3324459/150‑1 (MQ=255)
                                         cagTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAg                                                                                                           <  2:3872/150‑1 (MQ=255)
                                               gCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTg                                                                                                     <  2:55406/150‑1 (MQ=255)
                                                  gTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCg                                                                                                  <  2:2043749/150‑1 (MQ=255)
                                                    tAAACAGATAGGTAGACACCGTATCAGTAACGGAAACCTTTTTTGCAGGAACTTCTATCGCCATAAAACCATTGACCAGCGCACGCAGTTTTGCCTGTAACTTTGACTTGCTAGCGAACGACTGTTGGTGGCAAAACAGCACATGGCGGt                                                                                                <  1:1040785/150‑1 (MQ=255)
                                                    tAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGt                                                                                                <  2:3085778/150‑1 (MQ=255)
                                                       aCAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTAc                                                                                             <  1:4372576/150‑1 (MQ=255)
                                                          gATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCa                                                                                          <  2:74595/150‑1 (MQ=255)
                                                                  gACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCTCACGCAGGTTTGCCTGTAACATTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATc                                                                                  <  2:4217203/150‑1 (MQ=255)
                                                                  gACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATc                                                                                  >  2:2766155/1‑150 (MQ=255)
                                                                  gACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATc                                                                                  >  1:2746301/1‑150 (MQ=255)
                                                                   acacCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCa                                                                                 >  1:3050995/1‑150 (MQ=255)
                                                                      ccGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGt                                                                              <  2:4499060/150‑1 (MQ=255)
                                                                       cGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTc                                                                             <  2:4406366/150‑1 (MQ=255)
                                                                         tATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCAt                                                                           <  1:1063152/150‑1 (MQ=255)
                                                                            cAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTAt                                                                        >  1:886711/1‑150 (MQ=255)
                                                                             aGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATg                                                                       >  2:1150206/1‑150 (MQ=255)
                                                                                cacGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGaaa                                                                    >  2:4245339/1‑150 (MQ=255)
                                                                                 acGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGaaaa                                                                   >  2:3990347/1‑150 (MQ=255)
                                                                                    gAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACg                                                                >  2:1394707/1‑150 (MQ=255)
                                                                                      aaCCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGcc                                                              >  1:1325731/1‑150 (MQ=255)
                                                                                      aaCCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGcc                                                              <  1:3128399/150‑1 (MQ=255)
                                                                                            aGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTc                                                        >  2:1094644/1‑150 (MQ=255)
                                                                                               tGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGAt                                                     <  1:2217464/150‑1 (MQ=255)
                                                                                               tGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGAt                                                     <  1:2341788/150‑1 (MQ=255)
                                                                                                 ccGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATaa                                                   <  2:3791810/150‑1 (MQ=255)
                                                                                                 ccGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATaa                                                   <  2:1127918/150‑1 (MQ=255)
                                                                                                   ggAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACg                                                 >  2:2924727/1‑150 (MQ=255)
                                                                                                   ggAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACg                                                 >  2:1834159/1‑150 (MQ=255)
                                                                                                   ggAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACg                                                 >  2:1630334/1‑150 (MQ=255)
                                                                                                     aaCTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTc                                               <  2:1860103/150‑1 (MQ=255)
                                                                                                     aaCTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTc                                               <  2:679176/150‑1 (MQ=255)
                                                                                                      aCTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTcc                                              <  2:2577191/150‑1 (MQ=255)
                                                                                                      aCTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTcc                                              <  1:1686902/150‑1 (MQ=255)
                                                                                                       cTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCg                                             <  1:2997638/150‑1 (MQ=255)
                                                                                                        ttCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCgg                                            <  1:4189010/150‑1 (MQ=255)
                                                                                                         tCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCggg                                           >  2:3686269/1‑150 (MQ=255)
                                                                                                           tATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGtt                                         >  2:1370526/1‑150 (MQ=255)
                                                                                                           tATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGtt                                         >  2:2203221/1‑150 (MQ=255)
                                                                                                            aTCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGttt                                        >  2:582002/1‑150 (MQ=255)
                                                                                                            aTCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGttt                                        >  1:1743602/1‑150 (MQ=255)
                                                                                                            aTCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGttt                                        >  1:1018821/1‑150 (MQ=255)
                                                                                                             tCGCCATAAAACCATTGACCCGCGCACGCTGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGtttt                                       <  1:2574357/150‑1 (MQ=255)
                                                                                                             tCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGtttt                                       <  1:4169091/150‑1 (MQ=255)
                                                                                                             tCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGtttt                                       <  1:587221/150‑1 (MQ=255)
                                                                                                              cGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTg                                      >  2:390055/1‑150 (MQ=255)
                                                                                                              cGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTg                                      >  1:3279608/1‑150 (MQ=255)
                                                                                                                ccATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCt                                    >  1:3696904/1‑150 (MQ=255)
                                                                                                                ccATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCt                                    <  1:1540279/150‑1 (MQ=255)
                                                                                                                ccATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCt                                    >  1:3697005/1‑150 (MQ=255)
                                                                                                                ccATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCt                                    >  2:3420099/1‑150 (MQ=255)
                                                                                                                ccATAAAACCATGGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCt                                    <  1:2005496/150‑1 (MQ=255)
                                                                                                                 cATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTg                                   <  2:3389874/150‑1 (MQ=255)
                                                                                                                 cATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACGGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTg                                   <  2:3473906/150‑1 (MQ=255)
                                                                                                                 cATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTg                                   <  2:4226078/150‑1 (MQ=255)
                                                                                                                   tAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTggg                                 >  1:477790/1‑150 (MQ=255)
                                                                                                                         cATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGAGGCAAAACAGCACCTGGCGGTTACTCACGGCAAACACGTCATAATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGaaaa                           >  1:1355670/1‑150 (MQ=255)
                                                                                                                          aTTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAAt                          >  2:2325436/1‑150 (MQ=255)
                                                                                                                           ttGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATc                         <  1:4025950/150‑1 (MQ=255)
                                                                                                                            tGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCa                        <  1:2560046/150‑1 (MQ=255)
                                                                                                                             gACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCAc                       <  1:1629714/150‑1 (MQ=255)
                                                                                                                              aCCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCAcc                      >  1:1835156/1‑150 (MQ=255)
                                                                                                                               cccGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCt                     <  1:2702629/150‑1 (MQ=255)
                                                                                                                               cccGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCt                     >  1:1504345/1‑150 (MQ=255)
                                                                                                                                 cgcgCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGt                   <  2:3912410/150‑1 (MQ=255)
                                                                                                                                 cgcgCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGt                   <  1:3799440/150‑1 (MQ=255)
                                                                                                                                 cgcgCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGt                   >  2:3464264/1‑150 (MQ=255)
                                                                                                                                    gcacgcaGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTgg                <  2:2477787/150‑1 (MQ=255)
                                                                                                                                    gcacgcaGGTTTGCCTGAAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTgg                >  2:3184328/1‑150 (MQ=255)
                                                                                                                                     cacgcaGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTggg               >  1:4035817/1‑150 (MQ=255)
                                                                                                                                         caGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGAtt           <  2:45521/150‑1 (MQ=255)
                                                                                                                                         caGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGAtt           >  1:2766078/1‑150 (MQ=255)
                                                                                                                                         caGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGAtt           >  1:824457/1‑150 (MQ=255)
                                                                                                                                            gTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCAc        <  1:4462773/150‑1 (MQ=255)
                                                                                                                                              ttGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCt      >  1:4525209/1‑150 (MQ=255)
                                                                                                                                                gCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGa    <  1:1974103/150‑1 (MQ=255)
                                                                                                                                                  cAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGAtt  >  2:3550620/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTGATT  >  CP009273/1821549‑1821844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑