Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,850,880 C→A 21.9% V102V (GTG→GTT)  ydjH ← putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,850,8800CA21.9% 815.6 / 161.7 296V102V (GTG→GTT) ydjHputative kinase
Reads supporting (aligned to +/- strand):  ref base C (112/119);  new base A (36/29);  total (148/148)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 304 total aligned reads displayed.

TTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACG  >  CP009273/1850733‑1851029
                                                                                                                                                   |                                                                                                                                                     
tttACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAAc                                                                                                                                                     >  2:3109906/1‑150 (MQ=255)
  tACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCa                                                                                                                                                   <  2:4068318/150‑1 (MQ=255)
    ccATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGt                                                                                                                                                 >  1:4264028/1‑150 (MQ=255)
    ccATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGt                                                                                                                                                 >  2:3857739/1‑150 (MQ=255)
     cATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTc                                                                                                                                                >  1:4148620/1‑150 (MQ=255)
     cATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTc                                                                                                                                                >  2:1848456/1‑150 (MQ=255)
     cATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTc                                                                                                                                                <  2:2998923/150‑1 (MQ=255)
      aTCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTcc                                                                                                                                               <  1:1743562/150‑1 (MQ=255)
       tCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCa                                                                                                                                              <  2:1557472/150‑1 (MQ=255)
          aaTAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACg                                                                                                                                           >  2:2933105/1‑150 (MQ=255)
             aGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTa                                                                                                                                        <  1:4204308/150‑1 (MQ=255)
              gTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTaa                                                                                                                                       <  2:3253030/150‑1 (MQ=255)
                ggACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATa                                                                                                                                     <  1:1025900/150‑1 (MQ=255)
                ggACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATa                                                                                                                                     <  1:916271/150‑1 (MQ=255)
                      ttGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTa                                                                                                                               <  2:4264137/150‑1 (MQ=255)
                          aaaTACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTa                                                                                                                           >  2:786130/1‑150 (MQ=255)
                             tACTGGCCAGTGATAATAATTTCGCCTGAGTAAACCTATCAAAATCTACATCGTCAATATTCATTTTCCACAGACTGCCATTACGGTTTGTGAGAAACGTCCGCTCGCCATCCTCCGTCTCCTGTGCAACGTTTATAGTGGTATCTATgg                                                                                                                        <  2:4139842/150‑2 (MQ=255)
                                tGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACATACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATATAGGTATCTAATCTGa                                                                                                                     >  2:351400/1‑150 (MQ=255)
                                 ggCCAGTGATAATAATTTCGCATGAGAAAACCGCGAAAAATCAACAACGTAATTATTCAATTTCCACAGACTGCCATTAAGGTTTGTTAAAAACGTCCGCTCGCCATCCTCCGTCACCTGTCCAACGTTAATAGAGGTATCTATGCTGag                                                                                                                    <  1:3461616/150‑2 (MQ=255)
                                      gTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCt                                                                                                               >  1:2870614/1‑150 (MQ=255)
                                          taataaTTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCtt                                                                                                           <  1:3322545/150‑1 (MQ=255)
                                            ataatTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCa                                                                                                         >  1:900926/1‑150 (MQ=255)
                                            ataatTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCa                                                                                                         >  1:4418121/1‑150 (MQ=255)
                                            ataatTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCa                                                                                                         <  2:4580657/150‑1 (MQ=255)
                                            ataatTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCa                                                                                                         <  1:582688/150‑1 (MQ=255)
                                             taatTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAg                                                                                                        <  2:2257009/150‑1 (MQ=255)
                                                tttCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCt                                                                                                     >  1:3471409/1‑150 (MQ=255)
                                                 ttCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCtt                                                                                                    <  2:2261442/150‑1 (MQ=255)
                                                  tCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCttt                                                                                                   <  1:1092649/150‑1 (MQ=255)
                                                   cGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTg                                                                                                  >  1:2666757/1‑150 (MQ=255)
                                                     ccTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGaa                                                                                                <  2:1272946/150‑1 (MQ=255)
                                                     ccTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGaa                                                                                                <  2:933393/150‑1 (MQ=255)
                                                      cTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAat                                                                                               <  1:3077243/150‑1 (MQ=255)
                                                         agaAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATc                                                                                            <  1:292517/150‑1 (MQ=255)
                                                            aaaCCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAat                                                                                         >  1:2111817/1‑150 (MQ=255)
                                                            aaaCCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAat                                                                                         <  1:1202211/150‑1 (MQ=255)
                                                               ccGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATAtt                                                                                      <  1:800266/150‑1 (MQ=255)
                                                               ccGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATAtt                                                                                      <  2:2830340/150‑1 (MQ=255)
                                                                   gcAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTctct                                                                                  >  1:4485323/1‑150 (MQ=255)
                                                                     aaaaTCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCttt                                                                                >  1:470223/1‑150 (MQ=255)
                                                                       aaTCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGc                                                                              >  2:1249029/1‑150 (MQ=255)
                                                                        aTCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCg                                                                             >  1:4333731/1‑150 (MQ=255)
                                                                          cAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGc                                                                           >  1:4358169/1‑150 (MQ=255)
                                                                              aTCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATg                                                                       >  1:132212/1‑150 (MQ=255)
                                                                              aTCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCATGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATg                                                                       >  1:2222301/1‑150 (MQ=255)
                                                                               tCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATgg                                                                      >  2:2518562/1‑150 (MQ=255)
                                                                               tCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCGTTGCGGCAATgg                                                                      >  2:3874491/1‑150 (MQ=255)
                                                                                 gTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTc                                                                    <  1:2414825/150‑1 (MQ=255)
                                                                                  tCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCg                                                                   <  2:1229686/150‑1 (MQ=255)
                                                                                  tCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCg                                                                   >  2:4204639/1‑150 (MQ=255)
                                                                                   cAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCga                                                                  >  2:3729804/1‑150 (MQ=255)
                                                                                     atatTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCgaga                                                                <  2:2688989/150‑1 (MQ=255)
                                                                                     atatTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCgaga                                                                >  1:1001867/1‑150 (MQ=255)
                                                                                     atatTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTGTGCGGCAATGGTCgaga                                                                >  1:3725334/1‑150 (MQ=255)
                                                                                       atTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAAt                                                              <  2:1682963/150‑1 (MQ=255)
                                                                                        ttCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATa                                                             >  2:2275531/1‑150 (MQ=255)
                                                                                         tCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATaa                                                            >  1:3177353/1‑150 (MQ=255)
                                                                                         tCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATaa                                                            >  1:4427962/1‑150 (MQ=255)
                                                                                         tCAATTTCCACAGACTGCCATGACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATaa                                                            <  2:2145859/150‑1 (MQ=255)
                                                                                          cAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATaaa                                                           >  2:590905/1‑150 (MQ=255)
                                                                                            aTTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAAtt                                                         <  1:736835/150‑1 (MQ=255)
                                                                                               tCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGcc                                                      <  1:2318087/150‑1 (MQ=255)
                                                                                                  acaGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTg                                                   <  1:4559611/150‑1 (MQ=255)
                                                                                                    aGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCg                                                 >  1:991658/1‑150 (MQ=255)
                                                                                                    aGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCg                                                 >  1:3308667/1‑150 (MQ=255)
                                                                                                    aGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCg                                                 >  1:2575244/1‑150 (MQ=255)
                                                                                                    aGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCg                                                 <  1:3763571/150‑1 (MQ=255)
                                                                                                       cTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCa                                              >  1:807322/1‑150 (MQ=255)
                                                                                                       cTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCa                                              >  1:3606574/1‑150 (MQ=255)
                                                                                                       cTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCa                                              >  2:3583452/1‑150 (MQ=255)
                                                                                                        tGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCAt                                             <  1:2904458/150‑1 (MQ=255)
                                                                                                        tGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCAt                                             <  1:2696436/150‑1 (MQ=255)
                                                                                                         gCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATc                                            <  1:858888/150‑1 (MQ=255)
                                                                                                          ccATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCt                                           >  1:4608054/1‑150 (MQ=255)
                                                                                                           cATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCtt                                          >  2:1551877/1‑150 (MQ=255)
                                                                                                           cATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCtt                                          <  2:338926/150‑1 (MQ=255)
                                                                                                           cATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCtt                                          <  2:675642/150‑1 (MQ=255)
                                                                                                            aTTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCttt                                         >  2:191965/1‑150 (MQ=255)
                                                                                                              tACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTAc                                       <  1:361857/150‑1 (MQ=255)
                                                                                                                 ggTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCaa                                    >  2:494857/1‑150 (MQ=255)
                                                                                                                 ggTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCaa                                    >  1:1814150/1‑150 (MQ=255)
                                                                                                                   tttGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATa                                  <  2:3205532/150‑1 (MQ=255)
                                                                                                                    ttGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATAc                                 <  1:381304/150‑1 (MQ=255)
                                                                                                                    ttGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCCCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATAc                                 <  2:3831950/150‑1 (MQ=255)
                                                                                                                       tgACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGAc                              <  2:2188878/150‑1 (MQ=255)
                                                                                                                           aaaCGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCAt                          >  2:909347/1‑150 (MQ=255)
                                                                                                                           aaaCGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCAt                          >  1:3056007/1‑150 (MQ=255)
                                                                                                                            aaCGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCAtt                         >  2:4036471/1‑150 (MQ=255)
                                                                                                                               gTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAAt                      <  1:514320/150‑1 (MQ=255)
                                                                                                                                 ccGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGc                    <  1:1475156/150‑1 (MQ=255)
                                                                                                                                  cGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCt                   <  1:2030127/150‑1 (MQ=255)
                                                                                                                                  cGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCt                   >  1:2024052/1‑150 (MQ=255)
                                                                                                                                     tCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCtgtg                >  2:1080401/1‑150 (MQ=255)
                                                                                                                                       gCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCg              <  1:3628872/150‑1 (MQ=255)
                                                                                                                                           tcctccGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATgg          >  2:1463548/1‑150 (MQ=255)
                                                                                                                                             ctccGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGcc        >  2:3490756/1‑150 (MQ=255)
                                                                                                                                               ccGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCa      >  2:1009666/1‑150 (MQ=255)
                                                                                                                                                   cACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACg  <  1:461794/150‑1 (MQ=255)
                                                                                                                                                   cACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACg  <  1:4067287/150‑1 (MQ=255)
                                                                                                                                                   cACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACg  <  1:398855/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACG  >  CP009273/1850733‑1851029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑