BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,874,423	C→A	52.9%	L198F (TTG→TTT)	leuE ←	leucine efflux protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,874,423	0	C	A	52.9%	47.5 / inf	236	L198F (TTG→TTT)	leuE	leucine efflux protein
Reads supporting (aligned to +/- strand): ref base C (60/51); new base A (65/60); total (125/111)
Fisher's exact test for biased strand distribution p-value = 7.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 239 total aligned reads displayed.

TTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGT  >  CP009273/1874275‑1874572

 tGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATaaa                                                                                                                                                     <  2:2119864/150‑1 (MQ=255)

  gCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGACACCCTCGAACATCaaa                                                                                                                                                    >  2:2421608/1‑150 (MQ=255)

     ttttAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCa                                                                                                                                                 <  1:104924/150‑1 (MQ=255)

      tttAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCaa                                                                                                                                                <  2:2521269/150‑1 (MQ=255)

      tttAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCaa                                                                                                                                                >  1:553997/1‑150 (MQ=255)

       ttAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAAt                                                                                                                                               >  1:1434552/1‑150 (MQ=255)

       ttAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAAt                                                                                                                                               <  2:3066688/150‑1 (MQ=255)

        tAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATc                                                                                                                                              >  2:3789914/1‑150 (MQ=255)

          aaTACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAg                                                                                                                                            <  2:4299212/150‑1 (MQ=255)

          aaTACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAg                                                                                                                                            >  2:3206641/1‑150 (MQ=255)

          aaTACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAg                                                                                                                                            >  1:2472074/1‑150 (MQ=255)

          aaTACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGAGAAACCCACGAACATCAAACCAATCAg                                                                                                                                            <  2:4299196/150‑1 (MQ=255)

             aCCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGa                                                                                                                                         >  2:1274054/1‑150 (MQ=255)

              ccATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAg                                                                                                                                        >  1:3262034/1‑150 (MQ=255)

                aTTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGtt                                                                                                                                      >  2:3874264/1‑150 (MQ=255)

                aTTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGtt                                                                                                                                      <  1:3543391/150‑1 (MQ=255)

                   tATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGcc                                                                                                                                   <  2:2935764/150‑1 (MQ=255)

                      tGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAAc                                                                                                                                >  2:958294/1‑150 (MQ=255)

                        gTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTACAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACtt                                                                                                                              >  2:23536/1‑150 (MQ=255)

                         ttACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACttt                                                                                                                             >  1:3830961/1‑150 (MQ=255)

                          tACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTa                                                                                                                            <  1:4123917/150‑1 (MQ=255)

                           aCTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAg                                                                                                                           >  2:4314303/1‑150 (MQ=255)

                            cTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGc                                                                                                                          <  1:2392609/150‑1 (MQ=255)

                            cTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGc                                                                                                                          >  2:383271/1‑150 (MQ=255)

                             tttttAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGcc                                                                                                                         <  1:3136468/150‑1 (MQ=255)

                             tttttAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGcc                                                                                                                         >  1:1300355/1‑150 (MQ=255)

                                      ccATATCAGCGAAGAATCAGGGAGGATTACAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCttt                                                                                                                <  2:1248462/150‑1 (MQ=255)

                                         tatCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTg                                                                                                             <  2:2104777/150‑1 (MQ=255)

                                            cAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTa                                                                                                          <  1:4318563/150‑1 (MQ=255)

                                              gCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACg                                                                                                        >  1:3346756/1‑150 (MQ=255)

                                                gaagaaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTa                                                                                                      <  1:4027092/150‑1 (MQ=255)

                                                   gaaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGt                                                                                                   >  1:3234841/1‑150 (MQ=255)

                                                   gaaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGt                                                                                                   >  1:4539541/1‑150 (MQ=255)

                                                      tCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACt                                                                                                <  1:641461/150‑1 (MQ=255)

                                                      tCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACt                                                                                                >  1:616639/1‑150 (MQ=255)

                                                         gggAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCg                                                                                             >  2:1362313/1‑150 (MQ=255)

                                                          ggaggaTTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGt                                                                                            >  1:1541316/1‑150 (MQ=255)

                                                           gaggaTTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTg                                                                                           >  1:2813087/1‑150 (MQ=255)

                                                           gaggaTTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTg                                                                                           >  2:4132951/1‑150 (MQ=255)

                                                            aggaTTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGa                                                                                          <  1:3031592/150‑1 (MQ=255)

                                                              gaTTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACa                                                                                        >  1:896116/1‑150 (MQ=255)

                                                               aTTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACaa                                                                                       <  2:288497/150‑1 (MQ=255)

                                                                ttATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACaaa                                                                                      <  2:590691/150‑1 (MQ=255)

                                                                 tataGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACaaaa                                                                                     >  2:3087496/1‑150 (MQ=255)

                                                                  ataGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAg                                                                                    >  2:4004676/1‑150 (MQ=255)

                                                                   taGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGc                                                                                   >  1:4440045/1‑150 (MQ=255)

                                                                    aGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCa                                                                                  >  1:514295/1‑150 (MQ=255)

                                                                    aGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCa                                                                                  <  1:2247587/150‑1 (MQ=255)

                                                                       tGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCa                                                                               >  1:2086556/1‑150 (MQ=255)

                                                                       tGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCa                                                                               <  1:1917217/150‑1 (MQ=255)

                                                                        gggAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAg                                                                              <  2:4576121/150‑1 (MQ=255)

                                                                         ggAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGa                                                                             >  2:1695109/1‑150 (MQ=255)

                                                                          gAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGat                                                                            <  2:3384451/150‑1 (MQ=255)

                                                                           aaGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGata                                                                           >  1:1761900/1‑150 (MQ=255)

                                                                           aaGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGata                                                                           <  2:2832046/150‑1 (MQ=255)

                                                                              cccGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTNTGTACTGCGTGACAAAAGCACCAGATATaa                                                                        <  1:4281025/150‑1 (MQ=255)

                                                                                cgcgACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATc                                                                      <  2:548124/150‑1 (MQ=255)

                                                                                  cgACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAg                                                                    <  1:285636/150‑1 (MQ=255)

                                                                                   gACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAgg                                                                   >  2:3679239/1‑150 (MQ=255)

                                                                                    aCAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGa                                                                  >  1:1515722/1‑150 (MQ=255)

                                                                                      aaCGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAg                                                                >  1:2574162/1‑150 (MQ=255)

                                                                                       aCGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGc                                                               >  1:1038881/1‑150 (MQ=255)

                                                                                       aCGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGc                                                               >  1:2599591/1‑150 (MQ=255)

                                                                                       aCGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCACTCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGc                                                               <  2:2485145/150‑1 (MQ=255)

                                                                                        cgcgGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATACTCAGGAAGCt                                                              >  2:201267/1‑150 (MQ=255)

                                                                                         gcgGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTc                                                             <  1:1870889/150‑1 (MQ=255)

                                                                                           gggCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCaa                                                           >  2:555187/1‑150 (MQ=255)

                                                                                            ggCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCaaa                                                          >  2:834535/1‑150 (MQ=255)

                                                                                            ggCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCaaa                                                          >  2:3283207/1‑150 (MQ=255)

                                                                                            ggCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCaaa                                                          <  1:3789914/150‑1 (MQ=255)

                                                                                              cTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATa                                                        >  1:1449109/1‑150 (MQ=255)

                                                                                               tGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAg                                                       >  1:1015186/1‑150 (MQ=255)

                                                                                               tGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAg                                                       >  2:4464459/1‑150 (MQ=255)

                                                                                                   aGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGa                                                   <  2:344069/150‑1 (MQ=255)

                                                                                                     cATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGaaa                                                 <  2:2541019/150‑1 (MQ=255)

                                                                                                      aTCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAAc                                                >  2:4396568/1‑150 (MQ=255)

                                                                                                       tCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACt                                               >  1:3931532/1‑150 (MQ=255)

                                                                                                         aGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCa                                             >  2:746335/1‑150 (MQ=255)

                                                                                                          ggATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCAc                                            >  1:3833712/1‑150 (MQ=255)

                                                                                                           gATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCAcc                                           <  2:805105/150‑1 (MQ=255)

                                                                                                           gATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCAcc                                           <  2:4369880/150‑1 (MQ=255)

                                                                                                               gCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGtt                                       >  1:1390885/1‑150 (MQ=255)

                                                                                                                 aGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTcc                                     >  2:1452154/1‑150 (MQ=255)

                                                                                                                     tCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCg                                 >  1:758770/1‑150 (MQ=255)

                                                                                                                        ccAGTCGGGCAGCGAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTcg                              >  1:1430133/1‑150 (MQ=255)

                                                                                                                         cAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTcgc                             >  1:1942104/1‑150 (MQ=255)

                                                                                                                          aGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTcgcc                            <  1:2766216/150‑1 (MQ=255)

                                                                                                                               ggCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAg                       <  1:1396226/150‑1 (MQ=255)

                                                                                                                                   gCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATa                   <  1:4375241/150‑1 (MQ=255)

                                                                                                                                    cGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATaa                  <  1:3958538/150‑1 (MQ=255)

                                                                                                                                     gAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATaaa                 <  2:1139040/150‑1 (MQ=255)

                                                                                                                                     gAAACCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATaaa                 <  2:3320530/150‑1 (MQ=255)

                                                                                                                                      aaaCCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAg                <  2:4540461/150‑1 (MQ=255)

                                                                                                                                       aaCCCACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGa               >  1:411374/1‑150 (MQ=255)

                                                                                                                                        aCCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGaa              <  2:2599591/150‑1 (MQ=255)

                                                                                                                                        aCCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGaa              <  2:2298992/150‑1 (MQ=255)

                                                                                                                                        aCCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGaa              <  2:1038990/150‑1 (MQ=255)

                                                                                                                                         cccACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAAt             <  1:2429918/150‑1 (MQ=255)

                                                                                                                                          ccACGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATg            >  2:1399182/1‑150 (MQ=255)

                                                                                                                                            aCGAACATAAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGaa          <  2:179606/150‑1 (MQ=255)

TTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGT  >  CP009273/1874275‑1874572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`