Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,941,921 A→T 14.1% V163E (GTG→GAG)  yebC ← UPF0082 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,941,9210AT14.1% 808.6 / 108.8 277V163E (GTG→GAG) yebCUPF0082 family protein
Reads supporting (aligned to +/- strand):  ref base A (96/141);  new base T (18/21);  total (114/163)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 284 total aligned reads displayed.

CGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGAC  >  CP009273/1941772‑1942068
                                                                                                                                                     |                                                                                                                                                   
cGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTca                                                                                                                                                     <  2:3912639/150‑1 (MQ=255)
cGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTca                                                                                                                                                     <  2:3912632/150‑1 (MQ=255)
 ggTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTcac                                                                                                                                                    >  1:4114850/1‑150 (MQ=255)
    ttCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAAc                                                                                                                                                 <  2:4461368/150‑1 (MQ=255)
                   aGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGc                                                                                                                                  >  1:3501330/1‑150 (MQ=255)
                   aGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGc                                                                                                                                  >  1:1482933/1‑150 (MQ=255)
                     cTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCtt                                                                                                                                <  2:4320425/150‑1 (MQ=255)
                      tttGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTc                                                                                                                               >  2:1506236/1‑150 (MQ=255)
                      tttGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTc                                                                                                                               <  2:1096727/150‑1 (MQ=255)
                          gTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGt                                                                                                                           >  1:504478/1‑150 (MQ=255)
                            aGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGtgc                                                                                                                         <  1:2445340/150‑1 (MQ=255)
                              aCGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGtgctg                                                                                                                       >  1:1050327/1‑150 (MQ=255)
                              aCGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGtgctg                                                                                                                       <  1:2463146/150‑1 (MQ=255)
                                 ggATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCtt                                                                                                                    >  2:294011/1‑150 (MQ=255)
                                 ggATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCtt                                                                                                                    <  1:852418/150‑1 (MQ=255)
                                 ggATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCtt                                                                                                                    <  1:3188145/150‑1 (MQ=255)
                                  gATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTc                                                                                                                   >  1:3397313/1‑150 (MQ=255)
                                    tCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCa                                                                                                                 <  2:2763961/150‑1 (MQ=255)
                                     cATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCat                                                                                                                >  2:1593781/1‑150 (MQ=255)
                                       tGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCatga                                                                                                              >  2:2783709/1‑150 (MQ=255)
                                       tGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCatga                                                                                                              <  1:1475979/150‑1 (MQ=255)
                                       tGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCatga                                                                                                              <  1:1151269/150‑1 (MQ=255)
                                        ggAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCATCCAGTGCTGCATCCatgat                                                                                                             >  1:256463/1‑150 (MQ=255)
                                          aaaCTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATgg                                                                                                           <  1:3083043/150‑1 (MQ=255)
                                          aaaCTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATgg                                                                                                           <  1:2083955/150‑1 (MQ=255)
                                          aaaCTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATgg                                                                                                           <  1:3580794/150‑1 (MQ=255)
                                             cTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGgtgt                                                                                                        >  1:4045765/1‑150 (MQ=255)
                                               tCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCt                                                                                                      <  2:4291751/150‑1 (MQ=255)
                                               tCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCt                                                                                                      <  2:3728545/150‑1 (MQ=255)
                                                  gcgcTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCa                                                                                                   >  1:4478800/1‑150 (MQ=255)
                                                    gcTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATc                                                                                                 <  2:2149787/150‑1 (MQ=255)
                                                       gTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGcc                                                                                              >  2:1179245/1‑150 (MQ=255)
                                                         cTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCtt                                                                                            >  2:284459/1‑150 (MQ=255)
                                                              ttCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCCTGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCATTTCTCg                                                                                       <  2:779107/150‑1 (MQ=255)
                                                              ttCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCg                                                                                       <  2:1354206/150‑1 (MQ=255)
                                                                cAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGaa                                                                                     <  2:1586215/150‑1 (MQ=255)
                                                                  gACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAgg                                                                                   >  2:4570113/1‑150 (MQ=255)
                                                                   aCCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGga                                                                                  <  1:3513259/150‑1 (MQ=255)
                                                                   aCCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGga                                                                                  <  1:4458937/150‑1 (MQ=255)
                                                                   aCCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGga                                                                                  <  2:4449114/150‑1 (MQ=255)
                                                                   aCCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGga                                                                                  <  1:2650471/150‑1 (MQ=255)
                                                                      tGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGAt                                                                               <  2:3753658/150‑1 (MQ=255)
                                                                      tGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGAt                                                                               <  2:878302/150‑1 (MQ=255)
                                                                      tGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGAt                                                                               <  1:292001/150‑1 (MQ=255)
                                                                        ccGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCa                                                                             >  1:1087967/1‑150 (MQ=255)
                                                                          gCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACg                                                                           >  2:1967165/1‑150 (MQ=255)
                                                                          gCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACg                                                                           <  2:2560975/150‑1 (MQ=255)
                                                                               cAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCttt                                                                      <  1:4502709/150‑1 (MQ=255)
                                                                                    cGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGc                                                                 <  1:958455/150‑1 (MQ=255)
                                                                                     gTCGCGCACTTTACCCATTTCTTCCCATGCTGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTTCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCt                                                                <  2:416625/150‑1 (MQ=255)
                                                                                     gTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCt                                                                <  1:4259407/150‑1 (MQ=255)
                                                                                       cgcgCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGa                                                              >  2:3178376/1‑150 (MQ=255)
                                                                                          gcACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACa                                                           <  2:71868/150‑1 (MQ=255)
                                                                                           cACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAg                                                          >  1:3145949/1‑150 (MQ=255)
                                                                                           cACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAg                                                          >  1:122615/1‑150 (MQ=255)
                                                                                           cACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAg                                                          >  1:135589/1‑150 (MQ=255)
                                                                                             cTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGAt                                                        >  2:614211/1‑150 (MQ=255)
                                                                                               ttACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAg                                                      >  1:397745/1‑150 (MQ=255)
                                                                                                tACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAgg                                                     <  2:637018/150‑1 (MQ=255)
                                                                                                 aCCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGc                                                    >  2:404941/1‑150 (MQ=255)
                                                                                                   ccttttCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTTCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCaa                                                  <  1:728388/147‑1 (MQ=255)
                                                                                                        tcttcCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGaa                                             <  1:1020606/150‑1 (MQ=255)
                                                                                                         cttcCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAAc                                            <  2:1142358/150‑1 (MQ=255)
                                                                                                          ttcCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAAcc                                           <  1:1939424/150‑1 (MQ=255)
                                                                                                             ccATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATc                                        <  2:3144032/150‑1 (MQ=255)
                                                                                                              cATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCa                                       <  2:2967342/150‑1 (MQ=255)
                                                                                                              cATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCa                                       <  1:2181970/150‑1 (MQ=255)
                                                                                                              cATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCa                                       >  1:1204226/1‑150 (MQ=255)
                                                                                                                   aGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTAcc                                  <  2:4202869/150‑1 (MQ=255)
                                                                                                                   aGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTAcc                                  >  2:3242232/1‑150 (MQ=255)
                                                                                                                     tgtAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGa                                <  2:834746/150‑1 (MQ=255)
                                                                                                                      gtAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAg                               <  2:341108/150‑1 (MQ=255)
                                                                                                                      gtAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAg                               <  2:347681/150‑1 (MQ=255)
                                                                                                                      gtAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAg                               <  1:2368293/150‑1 (MQ=255)
                                                                                                                       tAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAgg                              >  1:2036810/1‑150 (MQ=255)
                                                                                                                        aGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGt                             >  2:888227/1‑150 (MQ=255)
                                                                                                                         gACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGtt                            <  1:140033/150‑1 (MQ=255)
                                                                                                                         gACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGtt                            <  2:530470/150‑1 (MQ=255)
                                                                                                                          aCATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTa                           <  2:3795912/150‑1 (MQ=255)
                                                                                                                            aTCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTAcc                         >  1:1579908/1‑150 (MQ=255)
                                                                                                                            aTCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTAcc                         <  2:2300003/150‑1 (MQ=255)
                                                                                                                            aTCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTAcc                         >  1:2741943/1‑150 (MQ=255)
                                                                                                                            aTCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTAcc                         >  2:24557/1‑150 (MQ=255)
                                                                                                                             tCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCg                        >  1:1703816/1‑150 (MQ=255)
                                                                                                                               aaTCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGcc                      >  2:1263544/1‑150 (MQ=255)
                                                                                                                                aTCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCca                     >  1:2226896/1‑150 (MQ=255)
                                                                                                                                    cgcCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACAtt                 <  2:1825796/150‑1 (MQ=255)
                                                                                                                                     gcCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACAttt                >  2:4122935/1‑150 (MQ=255)
                                                                                                                                     gcCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACAttt                >  1:333354/1‑150 (MQ=255)
                                                                                                                                        gTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCt             <  1:3908212/150‑1 (MQ=255)
                                                                                                                                          catcatAGGTCACAACGTCTTCAGCACCTGCTTCCTGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTaa           <  2:1660273/150‑1 (MQ=255)
                                                                                                                                          catcatAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTaa           <  2:21123/150‑1 (MQ=255)
                                                                                                                                           atcatAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTaaa          <  2:3885945/150‑1 (MQ=255)
                                                                                                                                             catAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATg        <  1:2896897/150‑1 (MQ=255)
                                                                                                                                              atAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGc       >  1:3565671/1‑150 (MQ=255)
                                                                                                                                              atAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGc       >  1:3950440/1‑150 (MQ=255)
                                                                                                                                              atAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGc       >  1:3257744/1‑150 (MQ=255)
                                                                                                                                                aGGTCACAACGTCTTCAGCACCTGCTCCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCAt     >  1:4406384/1‑150 (MQ=255)
                                                                                                                                                   tCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGAc  <  1:3476491/150‑1 (MQ=255)
                                                                                                                                                   tCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGAc  <  1:594203/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGAC  >  CP009273/1941772‑1942068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑