Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,946,006 G→T 43.0% D119Y (GAT→TAT)  yecE → UPF0759 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,946,0060GT43.0% 251.9 / 308.9 253D119Y (GAT→TAT) yecEUPF0759 family protein
Reads supporting (aligned to +/- strand):  ref base G (71/73);  new base T (43/66);  total (114/139)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 255 total aligned reads displayed.

CGCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGC  >  CP009273/1945857‑1946154
                                                                                                                                                     |                                                                                                                                                    
   aTCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTAtt                                                                                                                                                   <  2:2931750/150‑1 (MQ=255)
   aTCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTAtt                                                                                                                                                   >  1:2655943/1‑150 (MQ=255)
      aGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATtctc                                                                                                                                                <  2:693069/150‑1 (MQ=255)
         cagcaTTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTc                                                                                                                                             <  1:1108410/150‑1 (MQ=255)
          agcaTTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTcc                                                                                                                                            >  2:3393034/1‑150 (MQ=255)
          agcaTTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTcc                                                                                                                                            <  2:3287432/150‑1 (MQ=255)
           gcaTTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCt                                                                                                                                           <  1:1110579/150‑1 (MQ=255)
            caTTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTg                                                                                                                                          >  1:332761/1‑150 (MQ=255)
               tACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCg                                                                                                                                       >  2:1737729/1‑150 (MQ=255)
               tACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCg                                                                                                                                       <  1:1497615/150‑1 (MQ=255)
               tACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCg                                                                                                                                       <  1:3833499/150‑1 (MQ=255)
               tACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCg                                                                                                                                       >  2:4284250/1‑150 (MQ=255)
                  ggCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAAt                                                                                                                                    <  2:3826667/150‑1 (MQ=255)
                  ggCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAAt                                                                                                                                    >  1:1880389/1‑150 (MQ=255)
                   gCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAAtt                                                                                                                                   <  2:4570946/150‑1 (MQ=255)
                    cATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAAttt                                                                                                                                  <  2:4379689/150‑1 (MQ=255)
                      ttGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTaa                                                                                                                                <  1:1702785/150‑1 (MQ=255)
                      ttGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTaa                                                                                                                                <  1:1922566/150‑1 (MQ=255)
                        gCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAAtt                                                                                                                              <  1:4066173/150‑1 (MQ=255)
                          gatgatTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTAt                                                                                                                            <  1:2048827/150‑1 (MQ=255)
                           atgatTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATg                                                                                                                           >  1:2550050/1‑150 (MQ=255)
                           atgatTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATg                                                                                                                           >  2:3180496/1‑150 (MQ=255)
                                  aGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGa                                                                                                                    <  2:2053191/150‑1 (MQ=255)
                                   gTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGaa                                                                                                                   >  2:2407435/1‑150 (MQ=255)
                                    tGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAg                                                                                                                  >  2:2176977/1‑150 (MQ=255)
                                       cTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTcc                                                                                                               <  1:139363/150‑1 (MQ=255)
                                         gAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGc                                                                                                             >  2:4130930/1‑150 (MQ=255)
                                          aaTTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGcc                                                                                                            >  1:733442/1‑150 (MQ=255)
                                             ttttGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATc                                                                                                         >  1:26673/1‑150 (MQ=255)
                                                   cccGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGt                                                                                                   >  1:3527231/1‑150 (MQ=255)
                                                    ccGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGtt                                                                                                  <  2:1929849/150‑1 (MQ=255)
                                                     cGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGttt                                                                                                 <  1:2212778/150‑1 (MQ=255)
                                                      gCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGtttt                                                                                                <  2:3651108/150‑1 (MQ=255)
                                                      gCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGtttt                                                                                                <  1:2792986/150‑1 (MQ=255)
                                                      gCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGtttt                                                                                                <  1:641152/150‑1 (MQ=255)
                                                      gCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGtttt                                                                                                <  2:2612729/150‑1 (MQ=255)
                                                       cATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGttttt                                                                                               <  2:1605936/150‑1 (MQ=255)
                                                       cATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGttttt                                                                                               <  2:1678443/150‑1 (MQ=255)
                                                       cATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGttttt                                                                                               >  1:1507532/1‑150 (MQ=255)
                                                         tGTCACCGTTGGCTCCGCGCATTTGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCACACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCATGGCGAATTTAATTATGGAGTGGAAGTCCGCCATCCACAGTTTTTCg                                                                                             >  2:1012018/1‑150 (MQ=255)
                                                         tGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCg                                                                                             <  2:598480/150‑1 (MQ=255)
                                                          gTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGc                                                                                            >  2:558871/1‑150 (MQ=255)
                                                            cACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCa                                                                                          >  1:1733047/1‑150 (MQ=255)
                                                             aCCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCaa                                                                                         <  2:3766280/150‑1 (MQ=255)
                                                               cGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAg                                                                                       >  2:3005967/1‑150 (MQ=255)
                                                               cGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAg                                                                                       >  1:3590356/1‑150 (MQ=255)
                                                                gTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAgg                                                                                      <  2:4278324/150‑1 (MQ=255)
                                                                gTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAgg                                                                                      <  1:3631619/150‑1 (MQ=255)
                                                                gTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTCGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAgg                                                                                      >  1:3028107/1‑150 (MQ=255)
                                                                    gCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGagaa                                                                                  <  2:181794/150‑1 (MQ=255)
                                                                        cgcgCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAgg                                                                              >  2:1328563/1‑150 (MQ=255)
                                                                          cgcATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGaa                                                                            >  2:951625/1‑150 (MQ=255)
                                                                               tGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAAc                                                                       <  1:2671927/150‑1 (MQ=255)
                                                                                  gCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCt                                                                    >  1:1356511/1‑150 (MQ=255)
                                                                                    aaTACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTa                                                                  <  2:3670271/150‑1 (MQ=255)
                                                                                    aaTACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTa                                                                  <  1:3959203/150‑1 (MQ=255)
                                                                                     aTACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTaa                                                                 >  1:1803942/1‑150 (MQ=255)
                                                                                     aTACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTaa                                                                 <  1:4328749/150‑1 (MQ=255)
                                                                                      tACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAAt                                                                <  1:58666/150‑1 (MQ=255)
                                                                                          ggCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATcgcg                                                            <  1:494567/150‑1 (MQ=255)
                                                                                            cTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGt                                                          <  1:1266450/150‑1 (MQ=255)
                                                                                             tGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGtt                                                         <  1:1766308/150‑1 (MQ=255)
                                                                                               cAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTa                                                       >  2:3903173/1‑150 (MQ=255)
                                                                                                   tgcctgccACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATc                                                   >  1:4242205/1‑150 (MQ=255)
                                                                                                   tgcctgccACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATc                                                   >  1:269769/1‑150 (MQ=255)
                                                                                                         ccACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGcgcg                                             <  1:4361215/150‑1 (MQ=255)
                                                                                                          cacaTTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCgcgg                                            <  2:3157706/150‑1 (MQ=255)
                                                                                                           acaTTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACTTCAGCgcggc                                           <  1:4107708/150‑1 (MQ=255)
                                                                                                             aTTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGt                                         >  2:1053508/1‑150 (MQ=255)
                                                                                                              ttCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGtt                                        <  1:3690786/150‑1 (MQ=255)
                                                                                                                cGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTaa                                      >  1:2914853/1‑150 (MQ=255)
                                                                                                                 ggCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAAt                                     <  1:2731896/150‑1 (MQ=255)
                                                                                                                  gCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATc                                    <  2:1672136/150‑1 (MQ=255)
                                                                                                                   cccACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCg                                   <  2:4185487/150‑1 (MQ=255)
                                                                                                                        gggAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGa                              >  2:3564449/1‑150 (MQ=255)
                                                                                                                        gggAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGa                              >  1:2338003/1‑150 (MQ=255)
                                                                                                                         ggAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGAt                             <  2:395422/150‑1 (MQ=255)
                                                                                                                         ggAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGAt                             <  1:4417400/150‑1 (MQ=255)
                                                                                                                            gCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGAtttt                          <  1:2932802/150‑1 (MQ=255)
                                                                                                                             ctgcctgcGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTa                         <  1:210360/150‑1 (MQ=255)
                                                                                                                             ctgcctgcGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTa                         <  2:275536/150‑1 (MQ=255)
                                                                                                                              tgcctgcGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAg                        >  2:4118938/1‑150 (MQ=255)
                                                                                                                              tgcctgcGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAg                        <  1:688207/150‑1 (MQ=255)
                                                                                                                                cctgcGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGAc                      <  1:917346/150‑1 (MQ=255)
                                                                                                                                 ctgcGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACa                     <  2:873179/150‑1 (MQ=255)
                                                                                                                                   gcgcTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAgc                   <  1:3877032/150‑1 (MQ=255)
                                                                                                                                    cgcTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAgcc                  >  1:3394375/1‑150 (MQ=255)
                                                                                                                                    cgcTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAgcc                  <  2:898777/150‑1 (MQ=255)
                                                                                                                                      cTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAgccgc                >  2:4205501/1‑150 (MQ=255)
                                                                                                                                         ttgCATTTTCTCTAGTCTATTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGACATCCACAGTTTTTAGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTTCATCAGCGCGGCGTTAATCGTGTGATTTTAGACAgccgaacg             <  2:811621/148‑5 (MQ=255)
                                                                                                                                         tGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCg             <  2:403120/150‑1 (MQ=255)
                                                                                                                                         tGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCg             <  1:4622541/150‑1 (MQ=255)
                                                                                                                                         tGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCg             <  1:1931691/150‑1 (MQ=255)
                                                                                                                                            cATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGtt          >  1:1178409/1‑150 (MQ=255)
                                                                                                                                             aTTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTc         <  1:3080659/150‑1 (MQ=255)
                                                                                                                                              ttttCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCa        <  2:910432/150‑1 (MQ=255)
                                                                                                                                              ttttCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCa        >  1:3216379/1‑150 (MQ=255)
                                                                                                                                               tttCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCAt       >  2:3554232/1‑150 (MQ=255)
                                                                                                                                                  ctcGAGTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATgca    <  2:2273238/150‑1 (MQ=255)
                                                                                                                                                    cGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATgcagc  >  2:2903922/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAGC  >  CP009273/1945857‑1946154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑