Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,098,995 C→A 100% G182* (GGA→TGA)  wbbI ← d‑Galf:alpha‑d‑Glc beta‑1,6‑galactofuranosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,098,9950CA97.4% 771.1 / 10.0 229G182* (GGA→TGA) wbbId‑Galf:alpha‑d‑Glc beta‑1,6‑galactofuranosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (101/122);  total (104/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 231 total aligned reads displayed.

AGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACA  >  CP009273/2098848‑2099141
                                                                                                                                                   |                                                                                                                                                  
aGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCAtt                                                                                                                                                  <  1:3834820/150‑1 (MQ=255)
aGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCAtt                                                                                                                                                  >  1:2196721/1‑150 (MQ=255)
  gTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGTCACCAAAGAGAGTAAAATCGCATCATTCa                                                                                                                                                >  1:3332486/1‑150 (MQ=255)
   tttCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAg                                                                                                                                               <  2:4479576/150‑1 (MQ=255)
       gACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtata                                                                                                                                           <  1:3164808/150‑1 (MQ=255)
        aCAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtatat                                                                                                                                          <  2:3517832/150‑1 (MQ=255)
          aGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTatatat                                                                                                                                        >  2:4337555/1‑150 (MQ=255)
            aaTCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGa                                                                                                                                      >  1:3889328/1‑150 (MQ=255)
            aaTCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGa                                                                                                                                      >  1:3547787/1‑150 (MQ=255)
             aTCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGaa                                                                                                                                     <  2:198156/150‑1 (MQ=255)
              tctcCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGaaa                                                                                                                                    <  2:713918/150‑1 (MQ=255)
                 ccATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGaa                                                                                                                                 <  1:2018808/150‑1 (MQ=255)
                  cATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAAc                                                                                                                                <  1:1811560/150‑1 (MQ=255)
                   aTCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACa                                                                                                                               >  2:1370261/1‑150 (MQ=255)
                   aTCCCAAATGAGCCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACa                                                                                                                               >  1:1057494/1‑150 (MQ=255)
                     cccAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACAtt                                                                                                                             <  2:1778389/150‑1 (MQ=255)
                      ccAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACAttt                                                                                                                            <  2:4100866/150‑1 (MQ=255)
                            gAGTCCAAATTGCATGCCTGGGAGGTTAATCTTATCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATACATAATATATGAAAGAACATATATGCCt                                                                                                                      >  1:4313061/1‑150 (MQ=255)
                             aGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTa                                                                                                                     >  2:3131941/1‑150 (MQ=255)
                              gTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAg                                                                                                                    <  2:4399670/150‑1 (MQ=255)
                              gTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAg                                                                                                                    <  2:320585/150‑1 (MQ=255)
                                    aTTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGt                                                                                                              >  2:3733885/1‑150 (MQ=255)
                                    aTTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGt                                                                                                              >  2:1277084/1‑150 (MQ=255)
                                     ttGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGtt                                                                                                             <  1:3699275/150‑1 (MQ=255)
                                         aTGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCa                                                                                                         <  1:764371/150‑1 (MQ=255)
                                         aTGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCa                                                                                                         <  1:765026/150‑1 (MQ=255)
                                         aTGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCa                                                                                                         <  1:4384974/150‑1 (MQ=255)
                                             cTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCat                                                                                                     >  1:2603272/1‑150 (MQ=255)
                                              tGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCata                                                                                                    >  2:3483789/1‑150 (MQ=255)
                                              tGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCata                                                                                                    >  1:3538538/1‑150 (MQ=255)
                                                 gAGGTTAATCTTCTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCatatat                                                                                                 <  1:420980/150‑1 (MQ=255)
                                                  aGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATg                                                                                                >  2:1599122/1‑150 (MQ=255)
                                                        aTCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCt                                                                                          >  1:3654754/1‑150 (MQ=255)
                                                        aTCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCt                                                                                          >  1:1856876/1‑150 (MQ=255)
                                                         tCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTc                                                                                         <  2:437017/150‑1 (MQ=255)
                                                          cTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCg                                                                                        <  2:1554525/150‑1 (MQ=255)
                                                           ttttCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGt                                                                                       <  1:2705364/150‑1 (MQ=255)
                                                            tttCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGtt                                                                                      <  2:4045596/150‑1 (MQ=255)
                                                                 ggAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTa                                                                                 >  1:157510/1‑150 (MQ=255)
                                                                   agaTTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATc                                                                               >  2:1931126/1‑150 (MQ=255)
                                                                      ttGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGt                                                                            <  1:4171854/150‑1 (MQ=255)
                                                                          gCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACa                                                                        <  1:908493/150‑1 (MQ=255)
                                                                           cATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAt                                                                       >  1:4355388/1‑150 (MQ=255)
                                                                            aTCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAtc                                                                      >  1:1303783/1‑150 (MQ=255)
                                                                            aTCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAtc                                                                      >  1:987815/1‑150 (MQ=255)
                                                                            aTCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAtc                                                                      >  1:2868258/1‑150 (MQ=255)
                                                                            aTCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAtc                                                                      >  2:2196619/1‑150 (MQ=255)
                                                                             tCAAAACTTCCAAGATATTTAGGATTATCATTATTATCATAGTTGACACCAAAGAGAGTAAAATCGCAACATTCAGTATATATGAAAGAACATTTATGCCGAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAtct                                                                     >  2:701086/1‑150 (MQ=255)
                                                                               aaaaCTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCg                                                                   >  2:26597/1‑150 (MQ=255)
                                                                               aaaaCTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCg                                                                   >  2:3954150/1‑150 (MQ=255)
                                                                                     tCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTc                                                             <  1:1990430/150‑1 (MQ=255)
                                                                                       cAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCca                                                           <  1:2107804/150‑1 (MQ=255)
                                                                                       cAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCca                                                           >  1:3317097/1‑150 (MQ=255)
                                                                                         aGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCcaca                                                         >  1:534744/1‑150 (MQ=255)
                                                                                          gATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACAt                                                        <  1:1766418/150‑1 (MQ=255)
                                                                                           atatTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATc                                                       <  1:2598533/150‑1 (MQ=255)
                                                                                            tatTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCa                                                      <  1:1885901/150‑1 (MQ=255)
                                                                                             atTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAg                                                     <  1:2935245/150‑1 (MQ=255)
                                                                                                 aGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATga                                                 >  1:2535953/1‑150 (MQ=255)
                                                                                                   gATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATgaga                                               <  1:3985324/150‑1 (MQ=255)
                                                                                                   gATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATgaga                                               <  2:3618339/150‑1 (MQ=255)
                                                                                                   gATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATgaga                                               >  1:68604/1‑150 (MQ=255)
                                                                                                    aTTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGAc                                              <  2:2816641/150‑1 (MQ=255)
                                                                                                     ttATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACg                                             >  1:1489981/1‑150 (MQ=255)
                                                                                                      tATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGa                                            >  2:1973696/1‑150 (MQ=255)
                                                                                                      tATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGa                                            >  1:2232344/1‑150 (MQ=255)
                                                                                                        tCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAgg                                          <  2:3284425/150‑1 (MQ=255)
                                                                                                        tCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAgg                                          <  2:2778083/150‑1 (MQ=255)
                                                                                                         cTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGTCCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGt                                         >  2:4617974/1‑150 (MQ=255)
                                                                                                          tttATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTGTTCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTa                                        <  1:320338/150‑1 (MQ=255)
                                                                                                           ttATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTAGGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTaa                                       <  2:4261034/150‑1 (MQ=255)
                                                                                                            tATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAAt                                      <  1:1025349/150‑1 (MQ=255)
                                                                                                             aTTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATc                                     >  1:4566741/1‑150 (MQ=255)
                                                                                                             aTTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATc                                     <  2:1316394/150‑1 (MQ=255)
                                                                                                             aTTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTAGATCTGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATc                                     <  2:117663/150‑1 (MQ=255)
                                                                                                              ttttCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCa                                    <  1:3734982/150‑1 (MQ=255)
                                                                                                                  cATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAata                                >  2:2722970/1‑150 (MQ=255)
                                                                                                                  cATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAata                                >  2:2473556/1‑150 (MQ=255)
                                                                                                                  cATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAata                                <  1:2759504/150‑1 (MQ=255)
                                                                                                                   aTAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAatat                               <  1:2551497/150‑1 (MQ=255)
                                                                                                                       ttGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATAttttt                           <  1:827331/150‑1 (MQ=255)
                                                                                                                        tGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTa                          >  2:4186058/1‑150 (MQ=255)
                                                                                                                          acacCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGATTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATg                        <  2:4176473/150‑1 (MQ=255)
                                                                                                                            acCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATCTTTTTATGTc                      >  1:4481488/1‑150 (MQ=255)
                                                                                                                             ccAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAAACAAATATTTTTATGTCt                     >  2:1816409/1‑150 (MQ=255)
                                                                                                                               aaaGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCttt                   >  2:7776/1‑150 (MQ=255)
                                                                                                                               aaaGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCttt                   >  2:2426481/1‑150 (MQ=255)
                                                                                                                               aaaGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCttt                   >  2:103294/1‑150 (MQ=255)
                                                                                                                                   agagTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAtt               <  2:4186354/150‑1 (MQ=255)
                                                                                                                                    gagTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAttt              <  2:1649133/150‑1 (MQ=255)
                                                                                                                                    gagTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAttt              <  2:4015824/150‑1 (MQ=255)
                                                                                                                                     agTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAtttt             <  1:1941967/150‑1 (MQ=255)
                                                                                                                                      gTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTa            <  1:1005908/150‑1 (MQ=255)
                                                                                                                                       tAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTAt           <  1:2308164/150‑1 (MQ=255)
                                                                                                                                        aaaaTCGCATCATTCAGTATATATGAAAGAACCTTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATc          <  1:350282/150‑1 (MQ=255)
                                                                                                                                        aaaaACGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCATCATATATCACCCCACCTTGCTAATCAGTAACATATCGATTCTCCAAATCAGATGAGACGACGTAATCAAAAATTTTTATGGCTTTGATTTTATc          >  2:3469216/1‑150 (MQ=255)
                                                                                                                                         aaaTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAATGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATAAAATATTTTTATGTCTTTGATTTTATcc         <  1:2976942/150‑1 (MQ=255)
                                                                                                                                          aaTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCt        >  2:1250639/1‑150 (MQ=255)
                                                                                                                                             cGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTgag     >  1:526729/1‑150 (MQ=255)
                                                                                                                                                aTCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACa  <  2:3547859/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACA  >  CP009273/2098848‑2099141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑