Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,246,428 C→A 17.0% T399T (ACG→ACT)  nupX ← nucleoside permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,246,4280CA17.0% 786.4 / 97.4 251T399T (ACG→ACT) nupXnucleoside permease
Reads supporting (aligned to +/- strand):  ref base C (111/97);  new base A (19/24);  total (130/121)
Fisher's exact test for biased strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

GATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGC  >  CP009273/2246279‑2246577
                                                                                                                                                     |                                                                                                                                                     
gATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGc                                                                                                                                                       <  1:376631/150‑1 (MQ=255)
     gACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTcgc                                                                                                                                                  >  2:2253035/1‑150 (MQ=255)
      aCGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTcgcc                                                                                                                                                 <  1:1501328/150‑1 (MQ=255)
      aCGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTcgcc                                                                                                                                                 <  2:517947/150‑1 (MQ=255)
         cTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTcgccgcc                                                                                                                                              >  1:3226900/1‑150 (MQ=255)
          ttAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTcgccgccg                                                                                                                                             >  2:2484425/1‑150 (MQ=255)
               gcgTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAACTGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAgc                                                                                                                                        <  2:1181627/150‑1 (MQ=255)
               gcgTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAgc                                                                                                                                        <  1:4439654/150‑1 (MQ=255)
               gcgTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAgc                                                                                                                                        <  1:4439708/150‑1 (MQ=255)
               gcgTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAgc                                                                                                                                        <  2:2660043/150‑1 (MQ=255)
                  tCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGcc                                                                                                                                     >  2:3766096/1‑150 (MQ=255)
                     tATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGt                                                                                                                                  >  2:3910480/1‑150 (MQ=255)
                          ggCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAAcc                                                                                                                             >  1:4214465/1‑150 (MQ=255)
                           gCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCa                                                                                                                            <  1:4332767/150‑1 (MQ=255)
                              tACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGc                                                                                                                         <  1:512038/150‑1 (MQ=255)
                                cAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTg                                                                                                                       >  1:2463009/1‑150 (MQ=255)
                                 aaaTCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTgg                                                                                                                      >  2:2126621/1‑150 (MQ=255)
                                  aaTCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTggg                                                                                                                     <  1:3012497/150‑1 (MQ=255)
                                  aaTCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGCTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTggg                                                                                                                     <  1:3313992/150‑1 (MQ=255)
                                   aTCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGc                                                                                                                    <  2:2656804/150‑1 (MQ=255)
                                     cAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGa                                                                                                                  >  2:2327672/1‑150 (MQ=255)
                                     cAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGa                                                                                                                  >  2:4138367/1‑150 (MQ=255)
                                      aGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGAt                                                                                                                 <  1:3920934/150‑1 (MQ=255)
                                        gCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGAttt                                                                                                               >  1:3557927/1‑150 (MQ=255)
                                        gCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGAttt                                                                                                               <  2:211776/150‑1 (MQ=255)
                                         cAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTc                                                                                                              <  2:3051298/150‑1 (MQ=255)
                                         cAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTc                                                                                                              <  2:1086288/150‑1 (MQ=255)
                                          aaTTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCGATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTcc                                                                                                             >  1:2512628/1‑150 (MQ=255)
                                          aaTTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTcc                                                                                                             >  2:459284/1‑150 (MQ=255)
                                          aaTTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTcc                                                                                                             <  2:4609549/150‑1 (MQ=255)
                                          aaTTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTcc                                                                                                             >  1:3626552/1‑150 (MQ=255)
                                                  cAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACg                                                                                                     <  1:3701159/150‑1 (MQ=255)
                                                    aGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGgt                                                                                                   >  2:3211065/1‑150 (MQ=255)
                                                       aaaTGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGtgtg                                                                                                >  1:2091449/1‑150 (MQ=255)
                                                       aaaTGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGtgtg                                                                                                >  1:134227/1‑150 (MQ=255)
                                                        aaTGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCACCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTgg                                                                                               <  2:4226515/150‑1 (MQ=255)
                                                         aTGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGgc                                                                                              >  2:1919575/1‑150 (MQ=255)
                                                            tAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCa                                                                                           <  2:1404964/150‑1 (MQ=255)
                                                             aGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCaa                                                                                          >  2:135661/1‑150 (MQ=255)
                                                                ccTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCg                                                                                       >  1:2877172/1‑150 (MQ=255)
                                                                  tGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCa                                                                                     >  1:153119/1‑150 (MQ=255)
                                                                   gATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAg                                                                                    <  1:2785239/150‑1 (MQ=255)
                                                                    aTAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGa                                                                                   <  1:256265/150‑1 (MQ=255)
                                                                    aTAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGa                                                                                   >  1:2487918/1‑150 (MQ=255)
                                                                        gCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAAc                                                                               <  1:1204583/150‑1 (MQ=255)
                                                                        gCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAAc                                                                               <  1:1865569/150‑1 (MQ=255)
                                                                        gCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAAc                                                                               <  1:1571458/150‑1 (MQ=255)
                                                                         cTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACg                                                                              <  2:1630414/150‑1 (MQ=255)
                                                                         cTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACg                                                                              >  1:15573/1‑150 (MQ=255)
                                                                         cTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACg                                                                              <  1:2648212/150‑1 (MQ=255)
                                                                              gcATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGccccc                                                                         <  2:2974054/150‑1 (MQ=255)
                                                                               cATCAGTAAATGACACTCAAGCTAAACCAATAAAGAACCATGCAAAGGTCGCACTCATCAAGTTGGAAAGATTCGCCGCCGCCAGCGCACGTAAACCAATCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACACACcca                                                                        >  2:676013/1‑150 (MQ=255)
                                                                               cATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCcca                                                                        >  1:3493371/1‑150 (MQ=255)
                                                                                aTCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCccac                                                                       <  1:2004538/150‑1 (MQ=255)
                                                                                    ggCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCccaccacc                                                                   >  1:734961/1‑150 (MQ=255)
                                                                                    ggCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCccaccacc                                                                   >  1:4586983/1‑150 (MQ=255)
                                                                                      cAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCcaccaccac                                                                 >  1:3455870/1‑150 (MQ=255)
                                                                                        aTGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCAccc                                                               >  1:3874392/1‑150 (MQ=255)
                                                                                         tGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGATCAACCGCAGAAAACGCCCCCACCACCAcccc                                                              >  2:1629790/1‑150 (MQ=255)
                                                                                          gACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCg                                                             <  1:4207589/150‑1 (MQ=255)
                                                                                           acacTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGa                                                            >  2:3346041/1‑150 (MQ=255)
                                                                                            cacTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGAt                                                           <  1:4400816/150‑1 (MQ=255)
                                                                                             acTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATa                                                          <  1:3127214/150‑1 (MQ=255)
                                                                                                cAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGaa                                                       <  2:1727668/150‑1 (MQ=255)
                                                                                                 aaGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAAc                                                      <  1:691096/150‑1 (MQ=255)
                                                                                                  aGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAAcc                                                     <  2:4511122/150‑1 (MQ=255)
                                                                                                  aGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAAcc                                                     <  2:3614779/150‑1 (MQ=255)
                                                                                                     tAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCaaa                                                  <  1:1026210/150‑1 (MQ=255)
                                                                                                      aaaCCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAg                                                 <  2:900676/150‑1 (MQ=255)
                                                                                                       aaCCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGt                                                >  1:2928435/1‑150 (MQ=255)
                                                                                                       aaCCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGt                                                >  1:4533397/1‑150 (MQ=255)
                                                                                                       aaCCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGt                                                >  1:837490/1‑150 (MQ=255)
                                                                                                          cAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAg                                             >  1:4353742/1‑150 (MQ=255)
                                                                                                           aaTAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGc                                            >  2:3650652/1‑150 (MQ=255)
                                                                                                            aTAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCg                                           <  2:569247/150‑1 (MQ=255)
                                                                                                             tAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGa                                          >  1:2454436/1‑150 (MQ=255)
                                                                                                              aaaGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGaa                                         <  2:2462804/150‑1 (MQ=255)
                                                                                                                 gAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAAcc                                      <  2:4083287/150‑1 (MQ=255)
                                                                                                                 gAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAAcc                                      >  2:727886/1‑150 (MQ=255)
                                                                                                                 gAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAAcc                                      <  2:847322/150‑1 (MQ=255)
                                                                                                                         cAATGGTCGCACTCATCAAGTTGGAAAGAGNCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAAcg                              >  1:4279775/1‑150 (MQ=255)
                                                                                                                           aTGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAAcgcg                            <  1:138745/150‑1 (MQ=255)
                                                                                                                           aTGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAAcgcg                            <  1:2613200/150‑1 (MQ=255)
                                                                                                                             ggTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGaa                          >  2:614233/1‑150 (MQ=255)
                                                                                                                              gTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAg                         <  2:4005369/150‑1 (MQ=255)
                                                                                                                                 gCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGaa                      >  2:2370609/1‑150 (MQ=255)
                                                                                                                                 gCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGaa                      >  1:3252781/1‑150 (MQ=255)
                                                                                                                                  cACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGaaa                     >  1:4144415/1‑150 (MQ=255)
                                                                                                                                   aCTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAaat                    <  2:4347406/150‑1 (MQ=255)
                                                                                                                                    cTCATCAAGTTGGAACGCGTCGCCGCCGCCAGCGCCCGTAAACCCAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAaata                   <  1:921905/150‑1 (MQ=255)
                                                                                                                                    cTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAaata                   <  2:1899743/150‑1 (MQ=255)
                                                                                                                                       atctAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCTCCACCACCACGATAGAAACAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATc                <  2:2492228/150‑1 (MQ=25)
                                                                                                                                        tcaAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCg               <  1:2766275/150‑1 (MQ=255)
                                                                                                                                           aGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCca            >  1:352329/1‑150 (MQ=255)
                                                                                                                                             ttGTAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCcaca          <  1:805134/150‑1 (MQ=255)
                                                                                                                                               ggAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCACGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATATAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGt        <  1:4315420/150‑1 (MQ=255)
                                                                                                                                               ggAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGt        >  1:51939/1‑150 (MQ=255)
                                                                                                                                                  aaGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGtttt     >  2:866604/1‑150 (MQ=255)
                                                                                                                                                   aGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTa    <  2:2400611/150‑1 (MQ=255)
                                                                                                                                                    gCGTCGCCGCCGCCAGCGCCCGTAACCCAAGCTGGGCGATTTCCGGCGCACGGTGGGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAg   <  1:874432/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGC  >  CP009273/2246279‑2246577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑