Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,287,829 C→A 94.0% S185S (TCG→TCT)  ccmF ← heme lyase, CcmF subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,287,8290CA94.0% 763.7 / 39.4 250S185S (TCG→TCT) ccmFheme lyase, CcmF subunit
Reads supporting (aligned to +/- strand):  ref base C (7/8);  new base A (98/136);  total (105/145)
Fisher's exact test for biased strand distribution p-value = 7.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 251 total aligned reads displayed.

GAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAACAGCA  >  CP009273/2287682‑2287974
                                                                                                                                                   |                                                                                                                                                 
    tCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGaaa                                                                                                                                             <  2:4335729/150‑1 (MQ=255)
      gTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAAcc                                                                                                                                           >  2:3757770/1‑150 (MQ=255)
      gTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAAcc                                                                                                                                           <  2:3806522/150‑1 (MQ=255)
      gTAATAGGCCCAGGCGGAAGCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCCCGGACGAGTAAAACGCGCATAAGTGCTGTCCAGCCGCCCGCTCAGCAACGAAGCAATGGCAAAAGCAAACGCCACCGAGAAAcc                                                                                                                                           <  2:1120907/150‑1 (MQ=255)
        aaTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCa                                                                                                                                         <  2:117450/150‑1 (MQ=255)
        aaTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCa                                                                                                                                         >  2:1281199/1‑150 (MQ=255)
         aTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCAc                                                                                                                                        <  1:761694/150‑1 (MQ=255)
         aTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCAc                                                                                                                                        <  1:2088906/150‑1 (MQ=255)
           aGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGt                                                                                                                                      <  1:2142907/150‑1 (MQ=255)
                      gAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATaa                                                                                                                           <  1:1215129/150‑1 (MQ=255)
                        aCCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAg                                                                                                                         <  1:3828075/150‑1 (MQ=255)
                        aCCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAg                                                                                                                         <  1:3947014/150‑1 (MQ=255)
                         ccGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGc                                                                                                                        >  1:3603485/1‑150 (MQ=255)
                          cGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCa                                                                                                                       >  2:3379425/1‑150 (MQ=255)
                           gAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCag                                                                                                                      >  2:2699082/1‑150 (MQ=255)
                           gAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCag                                                                                                                      >  1:3179053/1‑150 (MQ=255)
                           gAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCag                                                                                                                      >  1:861168/1‑150 (MQ=255)
                              cACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAgg                                                                                                                   >  1:3636852/1‑150 (MQ=255)
                               aCGATGCCGAGCGTCATGAAGCTCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATATGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGc                                                                                                                  <  2:52954/150‑1 (MQ=255)
                               aCGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATATGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGc                                                                                                                  <  1:461113/150‑1 (MQ=255)
                               aCGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGc                                                                                                                  <  2:3027814/150‑1 (MQ=255)
                                 gATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCgg                                                                                                                <  2:1938579/150‑1 (MQ=255)
                                    gCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATg                                                                                                             <  2:56401/150‑1 (MQ=255)
                                       gAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGaa                                                                                                          <  1:2664511/150‑1 (MQ=255)
                                        aGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAg                                                                                                         <  2:2807741/150‑1 (MQ=255)
                                        aGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAg                                                                                                         <  1:4016494/150‑1 (MQ=255)
                                        aGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAg                                                                                                         <  2:3335799/150‑1 (MQ=255)
                                          cGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGAt                                                                                                       <  2:3039484/150‑1 (MQ=255)
                                          cGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGAt                                                                                                       >  1:1844143/1‑150 (MQ=255)
                                           gTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGATGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATc                                                                                                      >  2:3497637/1‑150 (MQ=255)
                                            tCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCa                                                                                                     <  2:2053346/150‑1 (MQ=255)
                                            tCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCa                                                                                                     <  1:435737/150‑1 (MQ=255)
                                             cAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAg                                                                                                    <  1:3395850/150‑1 (MQ=255)
                                             cAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAg                                                                                                    <  1:3735928/150‑1 (MQ=255)
                                              aGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGc                                                                                                   <  2:2940142/150‑1 (MQ=255)
                                                gAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGccc                                                                                                 >  1:2418181/1‑150 (MQ=255)
                                                  aGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAACCGCCACAGAGAAACCCACGGACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCg                                                                                               <  2:149369/150‑1 (MQ=255)
                                                       cACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATcc                                                                                          <  2:2554118/150‑1 (MQ=255)
                                                       cACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATcc                                                                                          <  1:3833280/150‑1 (MQ=255)
                                                            tGCCAGCGTCCACGGACGAGTAACACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGCAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACCTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCaa                                                                                     <  2:3986647/150‑1 (MQ=255)
                                                            tGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAACAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCaa                                                                                     <  2:3437885/150‑1 (MQ=255)
                                                             gCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAAt                                                                                    <  1:3020718/150‑1 (MQ=255)
                                                              ccAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATa                                                                                   <  1:1566963/150‑1 (MQ=255)
                                                               cAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAg                                                                                  >  1:586749/1‑150 (MQ=255)
                                                               cAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCGGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAg                                                                                  <  2:2247964/150‑1 (MQ=255)
                                                                aGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGc                                                                                 >  2:81888/1‑150 (MQ=255)
                                                                 gCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCg                                                                                <  1:1364492/150‑1 (MQ=255)
                                                                 gCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCg                                                                                <  1:2534237/150‑1 (MQ=255)
                                                                 gCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCg                                                                                <  2:1805305/150‑1 (MQ=255)
                                                                    tCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGt                                                                             <  1:169344/150‑1 (MQ=255)
                                                                    tCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGt                                                                             >  2:1555213/1‑150 (MQ=255)
                                                                      cACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTa                                                                           >  2:3264413/1‑150 (MQ=255)
                                                                          gACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGAt                                                                       >  1:900363/1‑150 (MQ=255)
                                                                            cGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATcg                                                                     <  1:869824/150‑1 (MQ=255)
                                                                            cGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATcg                                                                     >  1:3394675/1‑150 (MQ=255)
                                                                                        gcATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATc                                                         <  2:2446811/150‑1 (MQ=255)
                                                                                          aTAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCgg                                                       <  2:4566393/150‑1 (MQ=255)
                                                                                           tAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCggg                                                      <  1:463553/150‑1 (MQ=255)
                                                                                             aGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGaa                                                    <  1:528611/150‑1 (MQ=255)
                                                                                             aGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGaa                                                    >  1:4237544/1‑150 (MQ=255)
                                                                                              gTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAg                                                   <  2:2538377/150‑1 (MQ=255)
                                                                                               tGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGt                                                  <  2:2457398/150‑1 (MQ=255)
                                                                                                 cTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTc                                                <  1:1748325/150‑1 (MQ=255)
                                                                                                   gTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCgg                                              >  2:2249437/1‑150 (MQ=255)
                                                                                                    tCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGc                                             >  1:4148607/1‑150 (MQ=255)
                                                                                                    tCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGc                                             <  2:3171363/150‑1 (MQ=255)
                                                                                                        gACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGACGTTCGGCAACg                                         >  2:783895/1‑150 (MQ=255)
                                                                                                          cGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTg                                       >  1:4502053/1‑150 (MQ=255)
                                                                                                          cGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTg                                       <  1:2000985/150‑1 (MQ=255)
                                                                                                           gCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCACCGTGc                                      >  1:4555046/1‑150 (MQ=255)
                                                                                                           gCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGc                                      <  2:4359964/150‑1 (MQ=255)
                                                                                                            cccGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCg                                     <  1:4043765/150‑1 (MQ=255)
                                                                                                            cccGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCg                                     >  1:1536880/1‑150 (MQ=255)
                                                                                                            cccGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCg                                     <  1:798997/150‑1 (MQ=255)
                                                                                                               gCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCgaga                                  >  1:1001934/1‑150 (MQ=255)
                                                                                                               gCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCgaga                                  >  1:4599909/1‑150 (MQ=255)
                                                                                                                cTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCgagag                                 >  1:1126853/1‑150 (MQ=255)
                                                                                                                 tCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCgagaga                                >  2:1072609/1‑150 (MQ=255)
                                                                                                                  cAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGaa                               >  1:3717215/1‑150 (MQ=255)
                                                                                                                   aGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAAc                              >  1:13206/1‑150 (MQ=255)
                                                                                                                       aagaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGt                          >  1:2953184/1‑150 (MQ=255)
                                                                                                                       aagaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGt                          >  1:2303771/1‑150 (MQ=255)
                                                                                                                       aagaagCAAAGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCAAATAAAGCAGACGCGGATGGAAGAACAGCCCCGGAACCTGCAATAGCGGGTTAAGATCGCGACCTGCAATCGGGAAGATCGGCAACGAGCGAGAGCACCGGt                          >  2:3009211/1‑150 (MQ=255)
                                                                                                                        ataagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGtt                         <  1:598177/148‑1 (MQ=255)
                                                                                                                        agaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGtt                         <  2:3902109/150‑1 (MQ=255)
                                                                                                                        agaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGtt                         <  2:2318032/150‑1 (MQ=255)
                                                                                                                        agaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGtt                         <  1:4581151/150‑1 (MQ=255)
                                                                                                                        agaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGtt                         <  1:2911269/150‑1 (MQ=255)
                                                                                                                         gaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTa                        >  2:1773165/1‑150 (MQ=255)
                                                                                                                                tGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTGAGAGGTaa                 >  1:476442/1‑150 (MQ=255)
                                                                                                                                 ggCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTaaa                <  1:4195364/150‑1 (MQ=255)
                                                                                                                                  gCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAag               <  1:4188348/150‑1 (MQ=255)
                                                                                                                                    aaaaGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAagag             <  2:35672/150‑1 (MQ=255)
                                                                                                                                    aaaaGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAagag             >  1:3310527/1‑150 (MQ=255)
                                                                                                                                      aaGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGaa           <  2:4492007/150‑1 (MQ=255)
                                                                                                                                        gCAAACGCCACAGAGAAACCCACGTACCCCAGATCAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATg         <  2:4112554/150‑1 (MQ=255)
                                                                                                                                         cAAACGCCACAGAGAACCCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGa        <  2:718524/150‑1 (MQ=255)
                                                                                                                                          aaaCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGaa       <  1:976543/150‑1 (MQ=255)
                                                                                                                                          aaaCGCCACAGAGAAACCCACGTACCCCATAAAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGACGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGaa       >  1:313448/1‑150 (MQ=255)
                                                                                                                                           aaCGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAAc      <  1:1040496/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAACAGCA  >  CP009273/2287682‑2287974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑