Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,289,043 C→A 92.9% W8L (TGG→TTG)  ccmD ← cytochrome c biogenesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,289,0430CA92.9% 881.7 / 57.3 298W8L (TGG→TTG) ccmDcytochrome c biogenesis protein
Reads supporting (aligned to +/- strand):  ref base C (9/12);  new base A (129/147);  total (138/160)
Fisher's exact test for biased strand distribution p-value = 8.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 302 total aligned reads displayed.

TCACGCGCCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACC  >  CP009273/2288896‑2289189
                                                                                                                                                   |                                                                                                                                                  
 cACGCGACCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAgg                                                                                                                                                 >  2:259492/1‑150 (MQ=255)
  aCGCGCCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGa                                                                                                                                                >  2:4173892/1‑150 (MQ=255)
  aCGCGCCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGa                                                                                                                                                >  2:1087322/1‑150 (MQ=255)
     cgcCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGACTAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGc                                                                                                                                             >  1:3813123/1‑150 (MQ=255)
     cgcCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGc                                                                                                                                             <  1:3425752/150‑1 (MQ=255)
      gcCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCa                                                                                                                                            <  2:2380765/150‑1 (MQ=255)
       cccGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCaa                                                                                                                                           >  1:666399/1‑150 (MQ=255)
         cGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAAt                                                                                                                                         >  1:1435774/1‑150 (MQ=255)
          gCTTTTGCGCCACGCAACGCAGAATTGCGCTATGTTGCATCAACGAGAGCACGACCAAAACCACCAGCGGAATAAAGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGCACGCACATg                                                                                                                                        >  2:1850564/1‑150 (MQ=255)
          gCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCATAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATg                                                                                                                                        <  1:3848544/150‑1 (MQ=255)
          gCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATg                                                                                                                                        <  2:733071/150‑1 (MQ=255)
            tGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCa                                                                                                                                      <  1:1868587/150‑1 (MQ=255)
             gTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAg                                                                                                                                     <  1:1137991/150‑1 (MQ=255)
             gTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAg                                                                                                                                     <  1:3923385/150‑1 (MQ=255)
               tGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAggg                                                                                                                                   <  1:1719234/150‑1 (MQ=255)
                gcgcCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAgggg                                                                                                                                  <  1:1362814/150‑1 (MQ=255)
                gcgcCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAgggg                                                                                                                                  <  1:2575265/150‑1 (MQ=255)
                  gccacgccccGAAGAATTTCGAGATGTTGCATCTCCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCAAGCCAGACAAAAATGGCGGAACCGACCATTTCGAAAAATTCATTCAAGGAAGCAAATTCAGGGGTc                                                                                                                                >  2:813562/1‑150 (MQ=255)
                   ccacgccacgCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCa                                                                                                                               >  1:246257/1‑150 (MQ=255)
                      cgccacgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCAttt                                                                                                                            <  1:123315/150‑1 (MQ=255)
                          acgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACgg                                                                                                                        <  1:2861028/150‑1 (MQ=255)
                                  ttGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCtttt                                                                                                                <  2:2276385/150‑1 (MQ=255)
                                   tGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTc                                                                                                               <  2:4198735/150‑1 (MQ=255)
                                    gcgcGATGTTGCATCACCGAGTGCACGAACAAAACCAACAGCGTAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCa                                                                                                              <  2:2847336/150‑1 (MQ=255)
                                     cgcgATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAg                                                                                                             <  1:1276609/150‑1 (MQ=255)
                                      gcgATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGt                                                                                                            <  2:189275/150‑1 (MQ=255)
                                         aTGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATc                                                                                                         >  1:4238156/1‑150 (MQ=255)
                                         aTGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATc                                                                                                         <  2:1685373/150‑1 (MQ=255)
                                          tGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCa                                                                                                        >  2:3126624/1‑150 (MQ=255)
                                             tGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGtt                                                                                                     <  1:3337213/150‑1 (MQ=255)
                                             tGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGtt                                                                                                     <  1:101067/150‑1 (MQ=255)
                                             tGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGtt                                                                                                     <  1:2725003/150‑1 (MQ=255)
                                                aTCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAACATTCATTCAAGGAAGCTAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtcac                                                                                                  <  2:1544743/150‑1 (MQ=255)
                                                   aCCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtcactca                                                                                               <  2:4394343/150‑1 (MQ=255)
                                                      gAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCAccc                                                                                            >  2:3654066/1‑150 (MQ=255)
                                                      gAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCAccc                                                                                            >  2:384971/1‑150 (MQ=255)
                                                       aGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCa                                                                                           <  1:2375591/150‑1 (MQ=255)
                                                       aGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCa                                                                                           >  1:3752727/1‑150 (MQ=255)
                                                        gTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCac                                                                                          <  1:4343831/150‑1 (MQ=255)
                                                          gCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacgg                                                                                        <  1:2393192/150‑1 (MQ=255)
                                                            aCGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacggac                                                                                      >  1:4455390/1‑150 (MQ=255)
                                                             cGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacggacg                                                                                     >  1:1797911/1‑150 (MQ=255)
                                                             cGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacggacg                                                                                     <  1:1862551/150‑1 (MQ=255)
                                                              gACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacggacgg                                                                                    >  1:1598504/1‑150 (MQ=255)
                                                                ccAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCg                                                                                  <  1:2404517/150‑1 (MQ=255)
                                                                  aaaaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGtt                                                                                >  2:1245907/1‑150 (MQ=255)
                                                                      ccaccaGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTc                                                                            >  2:479110/1‑150 (MQ=255)
                                                                       caccaGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTcc                                                                           >  2:1535143/1‑150 (MQ=255)
                                                                         ccaGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCAt                                                                         >  2:3319187/1‑150 (MQ=255)
                                                                          caGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATc                                                                        <  2:960776/150‑1 (MQ=255)
                                                                               gAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCaa                                                                   >  1:3436196/1‑150 (MQ=255)
                                                                                 aTAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCaaaa                                                                 >  1:964573/1‑150 (MQ=255)
                                                                                 aTAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTGTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCaaaa                                                                 >  2:212375/1‑150 (MQ=255)
                                                                                       gTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAAt                                                           >  2:958411/1‑150 (MQ=255)
                                                                                          atcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAc                                                        >  1:4157387/1‑150 (MQ=255)
                                                                                            caccacCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgc                                                      >  2:2012920/1‑150 (MQ=255)
                                                                                             accaccGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgca                                                     >  1:4620140/1‑150 (MQ=255)
                                                                                             accaccGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgca                                                     >  2:4054383/1‑150 (MQ=255)
                                                                                              ccaccGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcat                                                    >  2:3916451/1‑150 (MQ=255)
                                                                                               caccGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatc                                                   >  1:1553031/1‑150 (MQ=255)
                                                                                                accGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatcc                                                  <  2:362155/150‑1 (MQ=255)
                                                                                                 ccGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccg                                                 <  2:458298/150‑1 (MQ=255)
                                                                                                   gccagccaGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccgca                                               <  2:3995702/150‑1 (MQ=255)
                                                                                                    ccagccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccgcat                                              >  2:2624618/1‑150 (MQ=255)
                                                                                                     cagccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccgcatc                                             >  1:3504813/1‑150 (MQ=255)
                                                                                                     cagccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccgcatc                                             >  2:2341879/1‑150 (MQ=255)
                                                                                                         cagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcg                                         >  2:2880242/1‑150 (MQ=255)
                                                                                                          agACAAAAAAGGCGTAACATCCCATTGCGTAAAATTAATTCAATTAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCATTTCTATCACCCACGGTCGGCGTTTTTCCATCAGCAAATTCTATTTACGCATCCGCAtcagcgt                                        <  2:1330948/150‑1 (MQ=255)
                                                                                                           gACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtc                                       <  2:3525316/150‑1 (MQ=255)
                                                                                                            aCAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtca                                      <  1:4173892/150‑1 (MQ=255)
                                                                                                            aCAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtca                                      >  1:163613/1‑150 (MQ=255)
                                                                                                             cAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAAGGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtcag                                     <  2:4345183/150‑1 (MQ=255)
                                                                                                              aaaaaaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtcagc                                    >  2:1298944/1‑150 (MQ=255)
                                                                                                                   aGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGc                               >  1:2238245/1‑150 (MQ=255)
                                                                                                                   aGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGc                               >  2:1750500/1‑150 (MQ=255)
                                                                                                                    ggCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCa                              <  2:2670169/150‑1 (MQ=255)
                                                                                                                      cGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGa                            >  2:3221276/1‑150 (MQ=255)
                                                                                                                       gTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGAc                           >  1:1450951/1‑150 (MQ=255)
                                                                                                                       gTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGAc                           <  1:4473601/150‑1 (MQ=255)
                                                                                                                         aaCCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAg                         >  2:2110862/1‑150 (MQ=255)
                                                                                                                         aaCCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAg                         >  2:3490157/1‑150 (MQ=255)
                                                                                                                          aCCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAgg                        <  1:2759639/150‑1 (MQ=255)
                                                                                                                           ccGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGa                       >  2:14609/1‑150 (MQ=255)
                                                                                                                              cccATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCa                    >  1:2978410/1‑150 (MQ=255)
                                                                                                                              cccATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCa                    >  2:1996299/1‑150 (MQ=255)
                                                                                                                               ccATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAg                   >  1:1381481/1‑150 (MQ=255)
                                                                                                                                  ttGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGaa                <  2:2060984/150‑1 (MQ=255)
                                                                                                                                   tGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCTCGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCTTCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAg               <  2:2043878/150‑1 (MQ=255)
                                                                                                                                   tGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCCCGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAg               <  2:1951367/150‑1 (MQ=255)
                                                                                                                                    gCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGc              <  2:3413591/150‑1 (MQ=255)
                                                                                                                                     cGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGcc             >  2:2424537/1‑150 (MQ=255)
                                                                                                                                      gAAAAATTCATTCTCGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCa            <  2:3733397/150‑1 (MQ=255)
                                                                                                                                      gAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCa            <  1:4358271/150‑1 (MQ=255)
                                                                                                                                        aaaaTTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaaa          <  2:3363761/150‑1 (MQ=255)
                                                                                                                                        aaaaTTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaaa          <  2:683414/150‑1 (MQ=255)
                                                                                                                                         aaaTTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaaaa         <  1:1475830/150‑1 (MQ=255)
                                                                                                                                            ttcattcaAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATc      <  1:805101/150‑1 (MQ=255)
                                                                                                                                             tcattcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCg     <  1:3160767/150‑1 (MQ=255)
                                                                                                                                             tcattcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCg     <  1:2276336/150‑1 (MQ=255)
                                                                                                                                             tcattcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCg     >  2:4503869/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCACGCGCCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACC  >  CP009273/2288896‑2289189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑