Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,352,573 C→A 100% P353P (CCG→CCT)  rhmT ← putative L‑rhamnonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,352,5730CA95.3% 953.1 / 34.5 297P353P (CCG→CCT) rhmTputative L‑rhamnonate transporter
Reads supporting (aligned to +/- strand):  ref base C (11/3);  new base A (144/139);  total (155/142)
Fisher's exact test for biased strand distribution p-value = 5.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 300 total aligned reads displayed.

TGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTC  >  CP009273/2352426‑2352722
                                                                                                                                                   |                                                                                                                                                     
  ccAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCa                                                                                                                                                   >  2:2397250/1‑150 (MQ=255)
    aGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATc                                                                                                                                                 <  1:4163122/150‑1 (MQ=255)
     gCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCg                                                                                                                                                <  1:777752/150‑1 (MQ=255)
      cGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGt                                                                                                                                               <  1:1193984/150‑1 (MQ=255)
      cGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGt                                                                                                                                               >  2:1937259/1‑150 (MQ=255)
      cGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGt                                                                                                                                               <  2:1989314/150‑1 (MQ=255)
           gTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGa                                                                                                                                          >  1:858665/1‑150 (MQ=255)
           gTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGa                                                                                                                                          >  1:3956208/1‑150 (MQ=255)
            tAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGaa                                                                                                                                         >  2:4593874/1‑150 (MQ=255)
             aaTCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAac                                                                                                                                        >  2:3366692/1‑150 (MQ=255)
                cccGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCTGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACt                                                                                                                                     <  1:3313368/150‑1 (MQ=255)
                cccGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACt                                                                                                                                     >  2:179868/1‑150 (MQ=255)
                     cgcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTg                                                                                                                                <  2:1587307/150‑1 (MQ=255)
                      tcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGc                                                                                                                               >  2:2066734/2‑150 (MQ=255)
                      gcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGc                                                                                                                               >  1:3897481/1‑150 (MQ=255)
                            cTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCa                                                                                                                         <  2:2789457/150‑1 (MQ=255)
                            cTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCa                                                                                                                         <  1:4192486/150‑1 (MQ=255)
                            cTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCa                                                                                                                         <  2:1412114/150‑1 (MQ=255)
                              ggCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAAt                                                                                                                       >  1:623766/1‑150 (MQ=255)
                              ggCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAAt                                                                                                                       <  1:496547/150‑1 (MQ=255)
                                  aaTAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAAc                                                                                                                   <  1:1573303/150‑1 (MQ=255)
                                    tAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAAccc                                                                                                                 <  1:3676030/150‑1 (MQ=255)
                                       cGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGAt                                                                                                              <  1:4017302/150‑1 (MQ=255)
                                        gTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATa                                                                                                             <  2:1227039/150‑1 (MQ=255)
                                        gTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATa                                                                                                             >  1:2321659/1‑150 (MQ=255)
                                        gTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATa                                                                                                             <  2:4155493/150‑1 (MQ=255)
                                          ttCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGc                                                                                                           <  1:2642087/150‑1 (MQ=255)
                                            cTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTg                                                                                                         <  1:1383077/150‑1 (MQ=255)
                                            cTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTg                                                                                                         >  2:912992/1‑150 (MQ=255)
                                                     cgcgCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAACCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAAcacaca                                                                                                <  2:4445824/150‑1 (MQ=255)
                                                     cgcgCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAAcacaca                                                                                                <  1:2149373/150‑1 (MQ=255)
                                                     cgcgCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAAcacaca                                                                                                <  1:3447305/150‑1 (MQ=255)
                                                     cgcgCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAAcacaca                                                                                                <  2:4445972/150‑1 (MQ=255)
                                                      gcgcAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAg                                                                                               <  1:3020603/150‑1 (MQ=255)
                                                         cAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgcgc                                                                                            <  1:2033981/150‑1 (MQ=255)
                                                         cAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgcgc                                                                                            >  2:4430406/1‑150 (MQ=255)
                                                         cAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgcgc                                                                                            <  1:1312884/150‑1 (MQ=255)
                                                           ggATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTa                                                                                          <  2:4023966/150‑1 (MQ=255)
                                                                ggggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATc                                                                                     >  2:1018248/1‑150 (MQ=255)
                                                                      aTAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCAtt                                                                               >  1:933519/1‑150 (MQ=255)
                                                                        aaaCCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTAc                                                                             >  2:2950012/1‑150 (MQ=255)
                                                                         aaCCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACt                                                                            <  1:1208688/150‑1 (MQ=255)
                                                                           cccGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTgg                                                                          <  1:2524568/150‑1 (MQ=255)
                                                                               cccACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGaa                                                                      >  1:4001102/1‑150 (MQ=255)
                                                                                     gCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAagcagc                                                                >  1:293934/1‑150 (MQ=255)
                                                                                     gCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAagcagc                                                                >  1:1032765/1‑150 (MQ=255)
                                                                                          aaaCAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGa                                                           <  1:4224196/150‑1 (MQ=255)
                                                                                           aaCAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGAc                                                          <  1:3874649/150‑1 (MQ=255)
                                                                                             cAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAg                                                        >  2:481046/1‑150 (MQ=255)
                                                                                                gTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGacc                                                     <  2:2465949/150‑1 (MQ=255)
                                                                                                  ttACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaa                                                   >  2:3535683/1‑150 (MQ=255)
                                                                                                   tACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaat                                                  >  1:4400951/1‑150 (MQ=255)
                                                                                                    aCAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaata                                                 >  2:3071751/1‑150 (MQ=255)
                                                                                                    aCAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaata                                                 >  2:1702505/1‑150 (MQ=255)
                                                                                                    aCAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaata                                                 >  1:4017488/1‑150 (MQ=255)
                                                                                                     cAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaatac                                                >  2:4381900/1‑150 (MQ=255)
                                                                                                      aaaaCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaatacc                                               >  2:1333934/1‑150 (MQ=255)
                                                                                                       aaaCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaatacca                                              >  2:4100963/1‑150 (MQ=255)
                                                                                                         aCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaataccaat                                            <  2:3832723/150‑1 (MQ=255)
                                                                                                         aCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaataccaat                                            >  1:1925243/1‑150 (MQ=255)
                                                                                                            aaTTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGcc                                         >  2:1044815/1‑150 (MQ=255)
                                                                                                            aaTTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGcc                                         >  2:2350982/1‑150 (MQ=255)
                                                                                                             aTTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGccc                                        <  1:3800717/150‑1 (MQ=255)
                                                                                                              ttCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccg                                       >  2:3847232/1‑150 (MQ=255)
                                                                                                              ttCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccg                                       <  2:3608457/150‑1 (MQ=255)
                                                                                                              ttCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccg                                       <  1:1723443/150‑1 (MQ=255)
                                                                                                              ttCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccg                                       <  2:2170912/150‑1 (MQ=255)
                                                                                                              ttCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccg                                       >  1:1934935/1‑150 (MQ=255)
                                                                                                               tCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccgc                                      >  2:618189/1‑150 (MQ=255)
                                                                                                                cccGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccgcc                                     >  2:435138/1‑150 (MQ=255)
                                                                                                                 ccGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccgccg                                    <  2:2277270/150‑1 (MQ=255)
                                                                                                                  cGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCcgccgc                                   >  2:4625253/1‑150 (MQ=255)
                                                                                                                  cGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCcgccgc                                   >  2:4082403/1‑150 (MQ=255)
                                                                                                                    cagccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCa                                 >  1:1011311/1‑150 (MQ=255)
                                                                                                                     agccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAg                                >  1:758614/1‑150 (MQ=255)
                                                                                                                     agccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCAGTACTGGAGAAAGCAGCCCGGACAGACCCATACCACTGCCCGCCGCCAg                                >  2:541225/1‑150 (MQ=255)
                                                                                                                     agcaagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAg                                >  1:2722520/1‑150 (MQ=255)
                                                                                                                         agcGCCGTACCGGAAAGCATTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGTCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCTGACAGACAAATACCAATGCCCGCCTCCAGTAAt                            >  2:3006841/1‑150 (MQ=255)
                                                                                                                         agcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCTATACCAATGCCCGCCGCCAGTAAt                            <  1:2433265/150‑1 (MQ=255)
                                                                                                                           cgcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGt                          >  2:3180724/1‑150 (MQ=255)
                                                                                                                           cgcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGt                          >  1:1858896/1‑150 (MQ=255)
                                                                                                                           cgcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGt                          >  2:1874502/1‑150 (MQ=255)
                                                                                                                           cgcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGt                          <  2:1198707/150‑1 (MQ=255)
                                                                                                                             ccGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCa                        <  1:1224929/150‑1 (MQ=255)
                                                                                                                             ccGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCa                        >  2:1939574/1‑150 (MQ=255)
                                                                                                                              cGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAg                       <  2:2844840/150‑1 (MQ=255)
                                                                                                                              cGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAg                       >  1:2896991/1‑150 (MQ=255)
                                                                                                                                tACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAgcg                     <  1:2054769/150‑1 (MQ=255)
                                                                                                                                  ccGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCt                   <  2:840273/150‑1 (MQ=255)
                                                                                                                                     gAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCg                >  2:3932693/1‑150 (MQ=255)
                                                                                                                                     gAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCg                <  1:1939306/150‑1 (MQ=255)
                                                                                                                                     gAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCg                <  1:4516284/150‑1 (MQ=255)
                                                                                                                                          cAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACAtt           <  1:4208069/150‑1 (MQ=255)
                                                                                                                                           aGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTa          >  1:1662767/1‑150 (MQ=255)
                                                                                                                                           aGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTa          <  2:749432/150‑1 (MQ=255)
                                                                                                                                               gtgtAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGcc      <  1:1107602/150‑1 (MQ=255)
                                                                                                                                               gtgtAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGcc      <  2:2630185/150‑1 (MQ=255)
                                                                                                                                               gtgtAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGcc      >  1:3005470/1‑150 (MQ=255)
                                                                                                                                                tgtAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCg     <  1:1818619/150‑1 (MQ=255)
                                                                                                                                                   cGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTc  <  1:2210288/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTC  >  CP009273/2352426‑2352722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑