Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,383,897 C→A 100% R363L (CGC→CTC)  nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,383,8970CA97.5% 677.7 / 7.1 203R363L (CGC→CTC) nuoNNADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base A (102/95);  total (103/100)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 203 total aligned reads displayed.

CCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCG  >  CP009273/2383750‑2384041
                                                                                                                                                   |                                                                                                                                                
 ccaccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGt                                                                                                                                               <  2:3654608/150‑1 (MQ=255)
  caccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTa                                                                                                                                              <  1:2317720/150‑1 (MQ=255)
      aGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACTATGCCAGAACAGACCGAGGTAagag                                                                                                                                          >  2:4051899/1‑150 (MQ=255)
      aGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAagag                                                                                                                                          >  1:596412/1‑150 (MQ=255)
      aGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGAGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACTATGCCACAACAGACCGAGGTCagag                                                                                                                                          >  2:1726213/1‑150 (MQ=255)
          accacAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGAGGCCAGAACAGACCGAGGTAAGAGATCa                                                                                                                                      <  2:716957/150‑1 (MQ=255)
                aGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAAt                                                                                                                                <  1:95621/150‑1 (MQ=255)
                 gtgtGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATc                                                                                                                               <  2:362391/150‑1 (MQ=255)
                   gtgCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAg                                                                                                                             >  1:1706264/1‑150 (MQ=255)
                   gtgCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAg                                                                                                                             >  1:1706381/1‑150 (MQ=255)
                    tgCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGc                                                                                                                            >  1:4237821/1‑150 (MQ=255)
                     gCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCa                                                                                                                           >  2:787565/1‑150 (MQ=255)
                       cTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATc                                                                                                                         <  1:4468303/150‑1 (MQ=255)
                        tGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATcc                                                                                                                        <  1:4120783/150‑1 (MQ=255)
                         ggACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCg                                                                                                                       <  2:4178339/150‑1 (MQ=255)
                          gACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCgg                                                                                                                      <  1:2197098/150‑1 (MQ=255)
                          gACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCgg                                                                                                                      <  2:1121023/150‑1 (MQ=255)
                            cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGc                                                                                                                    >  1:753159/1‑150 (MQ=255)
                                gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACg                                                                                                                >  2:2497229/1‑150 (MQ=255)
                                   cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATa                                                                                                             >  2:4274632/1‑150 (MQ=255)
                                      cAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACgg                                                                                                          <  2:3502090/150‑1 (MQ=255)
                                       aGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACggg                                                                                                         >  2:4212862/1‑150 (MQ=255)
                                        gCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGc                                                                                                        <  1:1457009/150‑1 (MQ=255)
                                         cACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCt                                                                                                       <  1:1946447/150‑1 (MQ=255)
                                          aCGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTg                                                                                                      <  2:337176/150‑1 (MQ=255)
                                            gTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGa                                                                                                    <  1:1899459/150‑1 (MQ=255)
                                            gTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGa                                                                                                    >  2:2267525/1‑150 (MQ=255)
                                            gTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGa                                                                                                    <  1:1899209/150‑1 (MQ=255)
                                             tAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGAc                                                                                                   <  2:3187611/150‑1 (MQ=255)
                                             tAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGAc                                                                                                   >  1:114538/1‑150 (MQ=255)
                                                aaCTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATc                                                                                                <  1:3367455/150‑1 (MQ=255)
                                                aaCTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATc                                                                                                <  1:900238/150‑1 (MQ=255)
                                                 aCTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCa                                                                                               <  2:4423622/150‑1 (MQ=255)
                                                   ttACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAgg                                                                                             <  1:2997676/150‑1 (MQ=255)
                                                   ttACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAgg                                                                                             <  1:136896/150‑1 (MQ=255)
                                                         aTAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGAcc                                                                                       >  1:3291577/1‑150 (MQ=255)
                                                          tAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCa                                                                                      <  1:4318416/150‑1 (MQ=255)
                                                              gCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGcc                                                                                  <  1:1355937/150‑1 (MQ=255)
                                                               cccAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCg                                                                                 <  2:2314133/150‑1 (MQ=255)
                                                               cccAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCg                                                                                 >  2:4453116/1‑150 (MQ=255)
                                                                ccAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGa                                                                                >  2:761372/1‑150 (MQ=255)
                                                                ccAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGa                                                                                >  1:3653278/1‑150 (MQ=255)
                                                                ccAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGa                                                                                <  2:2224531/150‑1 (MQ=255)
                                                                ccAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGa                                                                                >  1:3026962/1‑150 (MQ=255)
                                                                  aGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAc                                                                              <  1:239685/150‑1 (MQ=255)
                                                                  aGCGTCATCGGGAGACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGACCAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAc                                                                              <  1:4551241/150‑1 (MQ=255)
                                                                     gTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAcgcg                                                                           >  2:900595/1‑150 (MQ=255)
                                                                        aTCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCg                                                                        >  1:4499762/1‑150 (MQ=255)
                                                                        aTCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCg                                                                        >  1:1001580/1‑150 (MQ=255)
                                                                           gggATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAgg                                                                     >  1:2166088/1‑150 (MQ=255)
                                                                           gggATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAgg                                                                     >  2:1919759/1‑150 (MQ=255)
                                                                              aTACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGgctg                                                                  <  1:2574480/150‑1 (MQ=255)
                                                                              aTACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGgctg                                                                  >  2:2092560/1‑150 (MQ=255)
                                                                              aTACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGgctg                                                                  >  2:1497475/1‑150 (MQ=255)
                                                                                aCCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGgctgct                                                                <  2:4399223/150‑1 (MQ=255)
                                                                                  cGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGa                                                              <  2:1356479/150‑1 (MQ=255)
                                                                                     ccAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACa                                                           <  1:3788871/150‑1 (MQ=255)
                                                                                       agagACAGCATCATCACCGTCATCACTGCCGCGAGGATCGCACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTCACCACGCCGAACGCGCCGAGGCTGCTGAACAGa                                                         >  2:2903976/1‑150 (MQ=255)
                                                                                         agaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATa                                                       >  1:3425026/1‑150 (MQ=255)
                                                                                          gaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATaa                                                      >  1:2486807/1‑150 (MQ=255)
                                                                                           aCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAAc                                                     >  2:4377104/1‑150 (MQ=255)
                                                                                            cAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAAcc                                                    >  2:719854/1‑150 (MQ=255)
                                                                                             aGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCg                                                   >  1:3829350/1‑150 (MQ=255)
                                                                                                atcatcACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGcc                                                >  2:1204361/1‑150 (MQ=255)
                                                                                                atcatcACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGcc                                                >  2:1595886/1‑150 (MQ=255)
                                                                                                  catcaCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAg                                              >  1:3052885/1‑150 (MQ=255)
                                                                                                   atcaCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAgg                                             <  2:4168800/150‑1 (MQ=255)
                                                                                                     caCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa                                           >  1:214297/1‑150 (MQ=255)
                                                                                                      aCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTaa                                          <  2:2058751/150‑1 (MQ=255)
                                                                                                       ccGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTaaa                                         <  1:3775367/150‑1 (MQ=255)
                                                                                                         gTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAcc                                       >  2:504484/1‑150 (MQ=255)
                                                                                                         gTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAcc                                       >  2:2836838/1‑150 (MQ=255)
                                                                                                         gTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAcc                                       <  1:3619669/150‑1 (MQ=255)
                                                                                                          tcatcaCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAccc                                      <  2:4497393/150‑1 (MQ=255)
                                                                                                          tcatcaCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAccc                                      <  2:2869178/150‑1 (MQ=255)
                                                                                                          tcatcaCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAccc                                      >  1:934000/1‑150 (MQ=255)
                                                                                                           catcaCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAcccc                                     >  2:2995732/1‑150 (MQ=255)
                                                                                                             tcaCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTa                                   <  1:4212859/150‑1 (MQ=255)
                                                                                                               aCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTAcc                                 >  1:2750619/1‑150 (MQ=255)
                                                                                                                 tGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGc                               >  1:2109348/1‑150 (MQ=255)
                                                                                                                  gCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCt                              <  2:2414408/150‑1 (MQ=255)
                                                                                                                     gcgAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc                           <  1:3339226/150‑1 (MQ=255)
                                                                                                                      cgAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCa                          >  1:1517855/1‑150 (MQ=255)
                                                                                                                      cgAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCa                          <  2:1100633/150‑1 (MQ=255)
                                                                                                                          gATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGa                      <  1:1130840/150‑1 (MQ=255)
                                                                                                                           aTCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGAc                     <  2:3374330/150‑1 (MQ=255)
                                                                                                                           aTCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGAc                     >  1:1272203/1‑150 (MQ=255)
                                                                                                                           aTCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGAc                     <  2:1521349/150‑1 (MQ=255)
                                                                                                                              ggACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAtc                  >  1:1834115/1‑150 (MQ=255)
                                                                                                                              ggACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAtc                  >  1:2904437/1‑150 (MQ=255)
                                                                                                                                aCGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCATGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAtctc                <  1:4611080/150‑1 (MQ=255)
                                                                                                                                 cGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCg               <  1:3187871/150‑1 (MQ=255)
                                                                                                                                 cGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCg               >  1:2785113/1‑150 (MQ=255)
                                                                                                                                  gATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGc              >  1:2026859/1‑150 (MQ=255)
                                                                                                                                   aTGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGcc             <  2:1783189/150‑1 (MQ=255)
                                                                                                                                       cAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGtt         <  1:2479175/150‑1 (MQ=255)
                                                                                                                                        aGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGttt        >  2:1164248/1‑150 (MQ=255)
                                                                                                                                         gAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTg       >  2:2644910/1‑150 (MQ=255)
                                                                                                                                           aCAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCa     >  1:702382/1‑150 (MQ=255)
                                                                                                                                              gACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGTCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAgcg  >  2:879789/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCG  >  CP009273/2383750‑2384041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑