Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,412,205 C→A 92.8% D151Y (GAT→TAT)  yfcD ← putative NUDIX hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,412,2050CA92.8% 827.2 / 53.5 278D151Y (GAT→TAT) yfcDputative NUDIX hydrolase
Reads supporting (aligned to +/- strand):  ref base C (9/11);  new base A (131/127);  total (140/138)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 282 total aligned reads displayed.

CTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCT  >  CP009273/2412058‑2412351
                                                                                                                                                   |                                                                                                                                                  
cTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGc                                                                                                                                                  <  2:758633/150‑1 (MQ=255)
 tCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCa                                                                                                                                                 <  1:1530072/150‑1 (MQ=255)
 tCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCGTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGGCTGGAGTGAACTCATAGCa                                                                                                                                                 >  1:3159924/1‑150 (MQ=255)
   aGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATagcagc                                                                                                                                               >  2:3673323/1‑150 (MQ=255)
     ttGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGt                                                                                                                                             >  2:2406317/1‑150 (MQ=255)
      tGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTg                                                                                                                                            <  1:850821/150‑1 (MQ=255)
       gATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGc                                                                                                                                           <  2:1661027/150‑1 (MQ=255)
           ggCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTg                                                                                                                                       >  2:1258565/1‑150 (MQ=255)
            gCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGa                                                                                                                                      <  2:1412521/150‑1 (MQ=255)
               aCCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGAttt                                                                                                                                   >  1:558014/1‑150 (MQ=255)
                 cACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTGTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATttct                                                                                                                                 >  1:1713440/1‑150 (MQ=255)
                   cTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATttcttc                                                                                                                               >  1:241043/1‑150 (MQ=255)
                     aaaaaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCg                                                                                                                             >  1:509523/1‑150 (MQ=255)
                      aaaaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCgg                                                                                                                            >  1:1217588/1‑150 (MQ=255)
                       aaaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGc                                                                                                                           >  1:1316146/1‑150 (MQ=255)
                        aaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCg                                                                                                                          <  1:2825986/150‑1 (MQ=255)
                        aaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCg                                                                                                                          >  2:352569/1‑150 (MQ=255)
                        aaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCg                                                                                                                          >  1:3738065/1‑150 (MQ=255)
                           gTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTca                                                                                                                       >  1:4424053/1‑150 (MQ=255)
                             tAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTcagc                                                                                                                     >  1:1181965/1‑150 (MQ=255)
                               agagCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTcagcca                                                                                                                   <  1:2992916/150‑1 (MQ=255)
                                gagCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTcagccag                                                                                                                  <  2:1134503/150‑1 (MQ=255)
                                  gCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCa                                                                                                                <  1:2753430/150‑1 (MQ=255)
                                   ccGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCaa                                                                                                               >  1:494435/1‑150 (MQ=255)
                                      cAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACt                                                                                                            <  1:3203653/150‑1 (MQ=255)
                                       aaTTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACtt                                                                                                           >  2:3785781/1‑150 (MQ=255)
                                        aTTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTc                                                                                                          >  1:2218107/1‑150 (MQ=255)
                                        aTTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTc                                                                                                          >  2:3292931/1‑150 (MQ=255)
                                          tGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCAc                                                                                                        >  1:3675692/1‑150 (MQ=255)
                                          tGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCAc                                                                                                        >  1:3387464/1‑150 (MQ=255)
                                            cGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTACTTCCGGCGTCAGCCAGCAAACATCACTg                                                                                                      >  2:4572300/1‑150 (MQ=255)
                                              gCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGAc                                                                                                    >  1:1016012/1‑150 (MQ=255)
                                                   tttttCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCAt                                                                                               <  1:3295329/150‑1 (MQ=255)
                                                   tttttCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCAt                                                                                               <  1:249643/150‑1 (MQ=255)
                                                    ttttCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATc                                                                                              >  2:2164191/1‑150 (MQ=255)
                                                       tCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTc                                                                                           >  2:4348159/1‑150 (MQ=255)
                                                        cATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTcc                                                                                          >  2:4251258/1‑150 (MQ=255)
                                                         aTTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCt                                                                                         <  1:640196/150‑1 (MQ=255)
                                                         aTTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCt                                                                                         <  1:3946173/150‑1 (MQ=255)
                                                           tCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGt                                                                                       <  1:3103911/150‑1 (MQ=255)
                                                           tCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGt                                                                                       <  2:2083893/150‑1 (MQ=255)
                                                            cTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTa                                                                                      >  1:3007756/1‑150 (MQ=255)
                                                             tGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAg                                                                                     >  1:4410259/1‑150 (MQ=255)
                                                               ccGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTg                                                                                   >  1:3814873/1‑150 (MQ=255)
                                                                cGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCCTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTACATCTTCCTGTAGTGc                                                                                  >  1:4322963/1‑150 (MQ=255)
                                                                 gTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCg                                                                                 >  1:266899/1‑150 (MQ=255)
                                                                 gTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCg                                                                                 >  1:1241024/1‑150 (MQ=255)
                                                                 gTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCg                                                                                 >  2:4093242/1‑150 (MQ=255)
                                                                  tttCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGa                                                                                <  1:2142682/150‑1 (MQ=255)
                                                                   ttCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGaa                                                                               >  1:355621/1‑150 (MQ=255)
                                                                   ttCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGaa                                                                               >  1:2943594/1‑150 (MQ=255)
                                                                     cAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAgg                                                                             <  2:3746751/150‑1 (MQ=255)
                                                                     cAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAgg                                                                             >  1:4162302/1‑150 (MQ=255)
                                                                      aGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAggg                                                                            <  1:3768806/150‑1 (MQ=255)
                                                                      aGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAggg                                                                            <  2:3408893/150‑1 (MQ=255)
                                                                       gTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGa                                                                           <  1:4467772/150‑1 (MQ=255)
                                                                          tctACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCg                                                                        <  1:1390006/150‑1 (MQ=255)
                                                                          tctACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCg                                                                        <  2:2471875/150‑1 (MQ=255)
                                                                            tACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTg                                                                      >  1:101286/1‑150 (MQ=255)
                                                                             aCGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTga                                                                     >  1:1947434/1‑150 (MQ=255)
                                                                             aCGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTga                                                                     >  1:1876334/1‑150 (MQ=255)
                                                                                 cTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTgagaga                                                                 <  1:2164435/150‑1 (MQ=255)
                                                                                 cTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTgagaga                                                                 >  2:2686710/1‑150 (MQ=255)
                                                                                       ttttGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGc                                                           >  2:1988544/1‑150 (MQ=255)
                                                                                        tttGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCt                                                          <  2:2229973/150‑1 (MQ=255)
                                                                                         ttGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTg                                                         >  2:496949/1‑150 (MQ=255)
                                                                                          tGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGa                                                        <  2:3064453/150‑1 (MQ=255)
                                                                                           ggCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGaa                                                       >  1:1079185/1‑150 (MQ=255)
                                                                                            gCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAAc                                                      <  1:2636232/150‑1 (MQ=255)
                                                                                             cATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACa                                                     >  2:4273945/1‑150 (MQ=255)
                                                                                             cATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACa                                                     <  1:2673369/150‑1 (MQ=255)
                                                                                             cATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACa                                                     >  2:482566/1‑150 (MQ=255)
                                                                                             cATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACa                                                     <  1:595763/150‑1 (MQ=255)
                                                                                              aTTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACaa                                                    >  1:465731/1‑150 (MQ=255)
                                                                                              aTTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACaa                                                    >  1:708652/1‑150 (MQ=255)
                                                                                               ttGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAAt                                                   <  1:3097134/150‑1 (MQ=255)
                                                                                               ttGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAAt                                                   <  1:4327790/150‑1 (MQ=255)
                                                                                               ttGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAAt                                                   <  1:2119859/150‑1 (MQ=255)
                                                                                                  cgcTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATgcg                                                >  2:2295820/1‑150 (MQ=255)
                                                                                                  cgcTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATgcg                                                >  2:3519276/1‑150 (MQ=255)
                                                                                                   gcTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATgcgc                                               >  2:2088090/1‑150 (MQ=255)
                                                                                                       cATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCa                                           >  1:81534/1‑150 (MQ=255)
                                                                                                        aTCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAg                                          >  1:1798363/1‑150 (MQ=255)
                                                                                                               aCGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGc                                   <  2:2122550/150‑1 (MQ=255)
                                                                                                                  cTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAt                                >  2:1765548/1‑150 (MQ=255)
                                                                                                                   tAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAtt                               <  2:2336274/150‑1 (MQ=255)
                                                                                                                    aGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAttt                              <  1:1693829/150‑1 (MQ=255)
                                                                                                                    aGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAttt                              >  2:1939593/1‑150 (MQ=255)
                                                                                                                     gcgcTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAtttt                             <  2:276451/150‑1 (MQ=255)
                                                                                                                     gcgcTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAtttt                             <  2:1794184/150‑1 (MQ=255)
                                                                                                                     gcgcTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAtttt                             <  2:4120845/150‑1 (MQ=255)
                                                                                                                           tCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCtt                       <  2:4112651/150‑1 (MQ=255)
                                                                                                                                  gTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATa                <  2:833798/150‑1 (MQ=255)
                                                                                                                                     tGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGaa             <  2:3984327/150‑1 (MQ=255)
                                                                                                                                       gAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACt           <  1:206404/150‑1 (MQ=255)
                                                                                                                                         gTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGc         >  2:1518139/1‑150 (MQ=255)
                                                                                                                                         gTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGc         >  2:66233/1‑150 (MQ=255)
                                                                                                                                           gAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGccc       <  2:121028/150‑1 (MQ=255)
                                                                                                                                                cATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCt  <  1:4501594/150‑1 (MQ=255)
                                                                                                                                                cATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCt  <  1:322538/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCT  >  CP009273/2412058‑2412351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑