Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,456,508 A→T 36.0% S6S (TCA→TCT)  yfdF → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,456,5080AT36.0% 218.4 / 242.3 203S6S (TCA→TCT) yfdFuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (58/72);  new base T (43/30);  total (101/102)
Fisher's exact test for biased strand distribution p-value = 5.80e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 205 total aligned reads displayed.

CCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAA  >  CP009273/2456365‑2456656
                                                                                                                                               |                                                                                                                                                    
ccccTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATcaac                                                                                                                                                <  2:3263199/150‑1 (MQ=255)
ccccTGGCTATTGTGCGCTCATACACTCAAATAAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATcaac                                                                                                                                                <  2:3263252/150‑1 (MQ=255)
 cccTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATcaaca                                                                                                                                               <  2:1350384/150‑1 (MQ=255)
     ggCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATAc                                                                                                                                           >  2:1060926/1‑150 (MQ=255)
      gCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATAcc                                                                                                                                          <  1:743627/150‑1 (MQ=255)
        tATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAg                                                                                                                                        <  2:2276211/150‑1 (MQ=255)
        tATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAg                                                                                                                                        <  2:2620332/150‑1 (MQ=255)
           tgtgCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAgcgc                                                                                                                                     >  1:1766726/1‑150 (MQ=255)
            gtgCGCTCATACACTAAAATTACAGATAGTTTCTAAATAAATCAGCTTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGTATAATATCATGCTTCCATCTATTTCAATCAACAATACCAGCGCa                                                                                                                                    <  2:2634398/150‑1 (MQ=255)
               cgcTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCt                                                                                                                                 <  1:1334518/150‑1 (MQ=255)
                gcTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCtt                                                                                                                                >  2:3311772/1‑150 (MQ=255)
                 cTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTa                                                                                                                               >  2:3785019/1‑150 (MQ=255)
                 cTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTa                                                                                                                               <  2:464101/150‑1 (MQ=255)
                  tCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATCTTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTAc                                                                                                                              <  2:1150692/150‑1 (MQ=255)
                   cATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTAcc                                                                                                                             >  2:4302299/1‑150 (MQ=255)
                     tACACTCAAATTAAAGATAGGTTCTAAATACATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCa                                                                                                                           <  2:2292694/150‑1 (MQ=255)
                      acacTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAg                                                                                                                          <  2:4108766/150‑1 (MQ=255)
                          tCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATc                                                                                                                      <  1:4520091/150‑1 (MQ=255)
                           cAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATcc                                                                                                                     >  1:3700686/1‑150 (MQ=255)
                           cAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATcc                                                                                                                     <  2:1212932/150‑1 (MQ=255)
                           cAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATcc                                                                                                                     >  1:3771654/1‑150 (MQ=255)
                           cAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATcc                                                                                                                     >  1:3771398/1‑150 (MQ=255)
                            aaaTTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCa                                                                                                                    >  2:2683210/1‑150 (MQ=255)
                            aaaTTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCa                                                                                                                    >  2:1818548/1‑150 (MQ=255)
                            aaaTTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCa                                                                                                                    >  1:4463314/1‑150 (MQ=255)
                              aTTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATc                                                                                                                  >  1:127906/1‑150 (MQ=255)
                                 aaaGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAAt                                                                                                               >  2:1429557/1‑150 (MQ=255)
                                 aaaGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAAt                                                                                                               <  1:2556202/150‑1 (MQ=255)
                                   aGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGa                                                                                                             <  2:1882863/150‑1 (MQ=255)
                                    gATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGaa                                                                                                            >  1:270125/1‑150 (MQ=255)
                                     aTAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGTAATAAAATAATGATAACATCTATTTCAATAAACAATACCAGCGCAGCTTAACAAGAATAAATCAATGaaa                                                                                                           >  2:2478862/1‑150 (MQ=255)
                                     aTAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGaaa                                                                                                           <  2:156581/150‑1 (MQ=255)
                                          ttCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAAc                                                                                                      <  2:3825939/150‑1 (MQ=255)
                                           tCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACa                                                                                                     <  2:1678060/150‑1 (MQ=255)
                                           tCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACa                                                                                                     >  2:2379912/1‑150 (MQ=255)
                                             taaataaaTGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAat                                                                                                   >  1:3412447/1‑150 (MQ=255)
                                             taaataaaTGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAat                                                                                                   >  1:2207175/1‑150 (MQ=255)
                                             taaataaaTGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAat                                                                                                   <  2:600188/150‑1 (MQ=255)
                                             taaataaaTGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAat                                                                                                   >  1:2065109/1‑150 (MQ=255)
                                              aaataaatGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAatg                                                                                                  >  2:773144/1‑150 (MQ=255)
                                               aataaatGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAatga                                                                                                 <  1:4340951/150‑1 (MQ=255)
                                                 taaatGAGCGTTTTTTGATAGTTTATTTCATTATGTAATATATATTTTTATCAAATCAATAAAAATGGAATTAAATCTTGCTACCATCTTTTTGTATCAACAATACCAGAGCAGCTTACCCAGAATCCATCAATGAAAATAACActgatg                                                                                               <  2:698114/150‑1 (MQ=255)
                                                 taaatGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAatgatg                                                                                               <  1:64019/150‑1 (MQ=255)
                                                   aatGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGaa                                                                                             <  1:2986395/150‑1 (MQ=255)
                                                       aGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTa                                                                                         <  2:3575240/150‑1 (MQ=255)
                                                       aGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTa                                                                                         <  2:3577256/150‑1 (MQ=255)
                                                        gCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTaa                                                                                        <  1:1153154/150‑1 (MQ=255)
                                                         cGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAAt                                                                                       >  1:3613454/1‑150 (MQ=255)
                                                              tttGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGAtt                                                                                  <  1:664514/150‑1 (MQ=255)
                                                              tttGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGAtt                                                                                  <  2:1216122/150‑1 (MQ=255)
                                                               ttGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCATTGAAAATAACAATGATGAAGTTAATGGATTa                                                                                 <  2:4245430/150‑1 (MQ=255)
                                                               ttGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTa                                                                                 >  1:149231/1‑150 (MQ=255)
                                                                tGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAg                                                                                >  1:1416175/1‑150 (MQ=255)
                                                                 gATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGt                                                                               >  2:3502721/1‑150 (MQ=255)
                                                                     gTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACaa                                                                           >  1:3785354/1‑150 (MQ=255)
                                                                     gTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACaa                                                                           >  2:211036/1‑150 (MQ=255)
                                                                         aTTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGt                                                                       <  2:3030868/150‑1 (MQ=255)
                                                                          tttCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATTAAGTTAATGGATTAGTACAAGAGtt                                                                      <  2:2183809/150‑1 (MQ=255)
                                                                          tttCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGtt                                                                      <  1:4421328/150‑1 (MQ=255)
                                                                               ttAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCaaaa                                                                 >  1:1241758/1‑150 (MQ=255)
                                                                                  ggTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAAcc                                                              >  1:78720/1‑150 (MQ=255)
                                                                                       tatatatTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCttttt                                                         >  1:603219/1‑150 (MQ=255)
                                                                                       tatatatTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCttttt                                                         <  1:1437033/150‑1 (MQ=255)
                                                                                         tatatTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTaa                                                       <  1:766774/150‑1 (MQ=255)
                                                                                          atatTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAAt                                                      <  1:699310/150‑1 (MQ=255)
                                                                                          atatTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAAt                                                      <  1:2305672/150‑1 (MQ=255)
                                                                                              ttGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTa                                                  <  2:4062914/150‑1 (MQ=255)
                                                                                               tGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTaa                                                 <  1:3938857/150‑1 (MQ=255)
                                                                                               tGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTaa                                                 <  1:3748010/150‑1 (MQ=255)
                                                                                                 tAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTaagg                                               >  2:2188172/1‑150 (MQ=255)
                                                                                                  aaCAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTaagga                                              >  1:4592660/1‑150 (MQ=255)
                                                                                                    cAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTaaggaag                                            >  1:1574774/1‑150 (MQ=255)
                                                                                                        tcaatcaaAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAAt                                        >  1:2013273/1‑150 (MQ=255)
                                                                                                         caatcaaAATGGAATAAAATCATGCAACCAACTATTTCTATCAACAATACCAGCGCAGCTTACCAAGAATCCATCAATGAAAATAACACTGATGAAGTTAATGGATGAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATa                                       >  1:81333/1‑150 (MQ=255)
                                                                                                          aatcaaAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATaa                                      >  2:2741222/1‑150 (MQ=255)
                                                                                                          aatcaaAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATaa                                      >  1:251250/1‑150 (MQ=255)
                                                                                                          aatcaaAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATaa                                      <  1:4289482/150‑1 (MQ=255)
                                                                                                            tcaaAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGc                                    >  2:2152504/1‑150 (MQ=255)
                                                                                                              aaaaTGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCAc                                  <  1:3756540/150‑1 (MQ=255)
                                                                                                               aaaTGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCAcc                                 >  2:1114468/1‑150 (MQ=255)
                                                                                                                aaTGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCt                                <  2:2075402/150‑1 (MQ=255)
                                                                                                                  tGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGTAATAAGCACCTGt                              >  2:177087/1‑150 (MQ=255)
                                                                                                                     aaTAAAATCATGATACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAAACTTTTTAATGGTAAGGAAGGAATAAGCACCTGTCtt                           >  1:3281424/1‑150 (MQ=255)
                                                                                                                       tAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTaa                         >  1:2350822/1‑150 (MQ=255)
                                                                                                                          aaTCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACa                      <  1:841095/150‑1 (MQ=255)
                                                                                                                           aTCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTCTTAAACAt                     >  2:4091333/1‑150 (MQ=255)
                                                                                                                             cATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCt                   <  1:1913173/150‑1 (MQ=255)
                                                                                                                              aTGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTa                  >  1:1055025/1‑150 (MQ=255)
                                                                                                                               tGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTAc                 >  2:3980827/1‑150 (MQ=255)
                                                                                                                               tGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTAc                 >  1:4110478/1‑150 (MQ=255)
                                                                                                                               tGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTAc                 <  2:31420/150‑1 (MQ=255)
                                                                                                                                gCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACt                >  1:971759/1‑150 (MQ=255)
                                                                                                                                 cTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACtt               >  2:2908539/1‑150 (MQ=255)
                                                                                                                                 cTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACtt               <  2:3866046/150‑1 (MQ=255)
                                                                                                                                     cATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGc           >  1:1909771/1‑150 (MQ=255)
                                                                                                                                     cATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGc           >  2:1118056/1‑150 (MQ=255)
                                                                                                                                      aTCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCt          >  2:2804154/1‑150 (MQ=255)
                                                                                                                                        cTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTta        <  1:971046/150‑1 (MQ=255)
                                                                                                                                          aTTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTtata      >  1:2369982/1‑150 (MQ=255)
                                                                                                                                            ttCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATaaa    >  1:2941325/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAA  >  CP009273/2456365‑2456656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑