BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,457,130	C→A	7.8%	R214S (CGT→AGT)	yfdF →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,457,130	0	C	A	7.8%	856.4 / 30.5	226	R214S (CGT→AGT)	yfdF	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (125/83); new base A (7/11); total (132/94)
Fisher's exact test for biased strand distribution p-value = 8.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

GGAAAAGCGAACCAACGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGAAAGACTG  >  CP009273/2456981‑2457279

ggAAAAGCGAACCAACGGACTTATTTACCTGGTCCGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTc                                                                                                                                                       <  1:1202542/150‑1 (MQ=255)

ggAAAAGCGAACCAACGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTc                                                                                                                                                       >  2:1971440/1‑150 (MQ=255)

ggAAAAGCGAACCAACGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTc                                                                                                                                                       <  1:1634956/150‑1 (MQ=255)

  aaaaGCGAACCAACGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTAGt                                                                                                                                                     >  2:3231597/1‑150 (MQ=255)

        gacccAACGGACTTATTTACCTGGTATGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAAt                                                                                                                                               <  1:2851101/147‑1 (MQ=255)

        gAACCAACGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAAt                                                                                                                                               <  1:1046667/150‑1 (MQ=255)

        gAACCAACGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAAt                                                                                                                                               <  2:2133808/150‑1 (MQ=255)

        gAACCAACGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAAt                                                                                                                                               >  1:2971644/1‑150 (MQ=255)

         aaCAAACGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATg                                                                                                                                              <  2:761379/150‑1 (MQ=255)

             aaCGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGa                                                                                                                                          >  2:870665/1‑150 (MQ=255)

                 gACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGt                                                                                                                                      <  2:1038889/150‑1 (MQ=255)

                     tATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCa                                                                                                                                  >  2:220940/1‑150 (MQ=255)

                      aTTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTCTCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCAc                                                                                                                                 <  2:500345/150‑1 (MQ=255)

                      aTTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCAc                                                                                                                                 >  2:61562/1‑150 (MQ=255)

                        ttACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCAccc                                                                                                                               >  2:3250215/1‑150 (MQ=255)

                            cTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAata                                                                                                                           >  2:453887/1‑150 (MQ=255)

                                   ggTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGt                                                                                                                    >  1:1740480/1‑150 (MQ=255)

                                   ggTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGt                                                                                                                    >  1:1740244/1‑150 (MQ=255)

                                   ggTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGt                                                                                                                    <  2:1213629/150‑1 (MQ=255)

                                    gTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTg                                                                                                                   <  1:3785078/150‑1 (MQ=255)

                                     tAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGa                                                                                                                  >  2:1542049/1‑150 (MQ=255)

                                      aaaGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGaa                                                                                                                 >  1:4028714/1‑150 (MQ=255)

                                       aaGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGaaa                                                                                                                >  1:2657205/1‑150 (MQ=255)

                                          aTAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACg                                                                                                             <  1:4361873/150‑1 (MQ=255)

                                           tAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACACAATATTGTAGTGAAAACGc                                                                                                            >  2:1064136/1‑150 (MQ=255)

                                           tAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTAGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGc                                                                                                            >  1:2622383/1‑150 (MQ=255)

                                            aaaacaGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGcc                                                                                                           <  2:2709744/150‑1 (MQ=255)

                                            aaaaaaGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGcc                                                                                                           >  1:3098066/1‑150 (MQ=255)

                                                 aGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAg                                                                                                      <  2:2616150/150‑1 (MQ=255)

                                                   gCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTAGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTg                                                                                                    <  1:1277786/150‑1 (MQ=255)

                                                     gACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCt                                                                                                  >  2:3751714/1‑150 (MQ=255)

                                                        tctctcGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCa                                                                                               >  2:4304791/1‑150 (MQ=255)

                                                           ctcGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCg                                                                                            >  1:3576795/1‑150 (MQ=255)

                                                            tcGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGt                                                                                           >  2:3851593/1‑150 (MQ=255)

                                                             cGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTg                                                                                          >  1:4198717/1‑150 (MQ=255)

                                                                 aTTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGttt                                                                                      <  1:543067/150‑1 (MQ=255)

                                                                    gTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGaa                                                                                   >  2:4016752/1‑150 (MQ=255)

                                                                     tAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGaaa                                                                                  >  1:1026324/1‑150 (MQ=255)

                                                                       aTTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGc                                                                                <  1:1403063/150‑1 (MQ=255)

                                                                         taaaaataaaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAg                                                                              >  1:4090398/1‑150 (MQ=255)

                                                                             aataaaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTaaa                                                                          >  1:1318355/1‑150 (MQ=255)

                                                                             aataaaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTaaa                                                                          >  1:2151933/1‑150 (MQ=255)

                                                                                aaaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCt                                                                       <  2:1310500/150‑1 (MQ=255)

                                                                                aaaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCt                                                                       <  2:3954123/150‑1 (MQ=255)

                                                                                aaaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCt                                                                       <  1:658983/150‑1 (MQ=255)

                                                                                 aaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACTCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTa                                                                      >  1:3490212/1‑150 (MQ=255)

                                                                                 aaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTa                                                                      >  2:924822/1‑150 (MQ=255)

                                                                                 aaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTa                                                                      >  1:4501095/1‑150 (MQ=255)

                                                                                   aaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGGGGTTTGAAAGCAGTAAAGCTATc                                                                    >  2:1695373/1‑150 (MQ=255)

                                                                                    aCGTAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCa                                                                   <  2:4135539/150‑1 (MQ=255)

                                                                                    aCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTAGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCa                                                                   <  2:3066006/150‑1 (MQ=255)

                                                                                     cGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCAt                                                                  <  1:3370803/150‑1 (MQ=255)

                                                                                     cGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCAt                                                                  >  1:3633951/1‑150 (MQ=255)

                                                                                      ggAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATg                                                                 >  1:2954/1‑150 (MQ=255)

                                                                                        aaaTGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGc                                                               >  1:2839413/1‑150 (MQ=255)

                                                                                         aaTGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCa                                                              <  1:4410746/150‑1 (MQ=255)

                                                                                             aTTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAAt                                                          >  2:2952573/1‑150 (MQ=255)

                                                                                             aTTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAAt                                                          >  2:2778997/1‑150 (MQ=255)

                                                                                               tACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCa                                                        >  1:3858150/1‑150 (MQ=255)

                                                                                                aCTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAt                                                       >  2:2658267/1‑150 (MQ=255)

                                                                                                 cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAtt                                                      >  1:1089068/1‑150 (MQ=255)

                                                                                                 cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAtt                                                      <  2:3381190/150‑1 (MQ=255)

                                                                                                  ttATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAttt                                                     >  2:1876552/1‑150 (MQ=255)

                                                                                                  ttATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAttt                                                     <  2:1973537/150‑1 (MQ=255)

                                                                                                  ttATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAttt                                                     <  1:1478592/150‑1 (MQ=255)

                                                                                                   tATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTg                                                    >  1:2476190/1‑150 (MQ=255)

                                                                                                    aTCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTAGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGc                                                   <  1:4620093/150‑1 (MQ=255)

                                                                                                       tctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAAc                                                <  2:2079129/150‑1 (MQ=255)

                                                                                                        ctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTAGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACt                                               <  1:1470681/150‑1 (MQ=255)

                                                                                                         tcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTg                                              <  2:349976/150‑1 (MQ=255)

                                                                                                         tcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTg                                              <  2:2800226/150‑1 (MQ=255)

                                                                                                         tcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTg                                              <  1:733884/150‑1 (MQ=255)

                                                                                                           ggTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGt                                            >  2:3365367/1‑150 (MQ=255)

                                                                                                              tactacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTcc                                         <  2:2507544/150‑1 (MQ=255)

                                                                                                                ctactaTCAGGACGACTACCACATTCAAAGAGGAATTAGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGa                                       <  1:1995761/150‑1 (MQ=255)

                                                                                                                ctacGATCAGGACGACTACCACATTCAAAGAGTAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGa                                       <  1:3936910/150‑1 (MQ=255)

                                                                                                                  acGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatc                                     >  1:713299/1‑150 (MQ=255)

                                                                                                                      tCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGAtcatca                                 <  2:3561990/150‑1 (MQ=255)

                                                                                                                        aGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCCTCAgg                               >  2:2033183/1‑150 (MQ=255)

                                                                                                                            cgacTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGt                           >  1:2343739/1‑150 (MQ=255)

                                                                                                                              acTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTaa                         >  1:3832776/1‑150 (MQ=255)

                                                                                                                               cTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTaaa                        <  2:880478/150‑1 (MQ=255)

                                                                                                                               cTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTaaa                        >  1:3532101/1‑150 (MQ=255)

                                                                                                                               cTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTaaa                        >  2:4377224/1‑150 (MQ=255)

                                                                                                                                tACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGGAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAAc                       <  2:2282604/150‑1 (MQ=255)

                                                                                                                                  ccACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGa                     <  2:773698/150‑1 (MQ=255)

                                                                                                                                   cacaTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAg                    >  1:4235569/1‑150 (MQ=255)

                                                                                                                                    acaTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGc                   <  1:3673700/150‑1 (MQ=255)

                                                                                                                                     caTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCt                  <  1:2013576/150‑1 (MQ=255)

                                                                                                                                           aaGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACgg            >  1:3984503/1‑150 (MQ=255)

                                                                                                                                           aaGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACgg            >  2:566541/1‑150 (MQ=255)

                                                                                                                                             gagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACgggg          <  2:4177804/150‑1 (MQ=255)

                                                                                                                                              agGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGa         >  1:2743937/1‑150 (MQ=255)

                                                                                                                                               ggAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGaa        <  2:2082292/150‑1 (MQ=255)

                                                                                                                                                gAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGaaa       >  2:461846/1‑150 (MQ=255)

                                                                                                                                                 aaTTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGAAAg      >  2:1283081/1‑150 (MQ=255)

                                                                                                                                                  aTTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTCCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGAAAGa     >  1:2248104/1‑150 (MQ=255)

                                                                                                                                                  aTTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTCCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGAAAGa     >  1:1866120/1‑150 (MQ=255)

                                                                                                                                                   ttCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGAAAGAc    >  2:3689121/1‑150 (MQ=255)

                                                                                                                                                     cGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGAAAGACTg  >  2:1674808/1‑150 (MQ=255)

GGAAAAGCGAACCAACGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGAAAGACTG  >  CP009273/2456981‑2457279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`