Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,463,760 T→A 36.8% V384E (GTA→GAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,463,7600TA36.8% 229.9 / 238.2 209V384E (GTA→GAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base T (68/64);  new base A (39/38);  total (107/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

CACGTAATATATGGAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATT  >  CP009273/2463612‑2463905
                                                                                                                                                    |                                                                                                                                                 
cacGTAATATATGGAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTgta                                                                                                                                                  >  1:2130485/1‑150 (MQ=255)
   gTAATATATGGAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTgtagta                                                                                                                                               >  2:3870959/1‑150 (MQ=255)
   gTAATATATGGAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTgaagta                                                                                                                                               >  1:4015056/1‑150 (MQ=255)
   gTAATATATGGAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATAATTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATGAGAAATAATAAAATAAAGGCATCTTTATTTgtagta                                                                                                                                               <  2:328030/150‑1 (MQ=255)
   caaaaatagGGAGAAACAAACATACTACCGACCATTACTAACATAAAAGACAATATATTAAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTgtagta                                                                                                                                               <  2:3442567/145‑1 (MQ=255)
    tAATATATGGAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTAt                                                                                                                                              >  1:4449107/1‑150 (MQ=255)
    tAATATATGGAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGATTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTAt                                                                                                                                              <  1:1902801/150‑1 (MQ=255)
    tAATATAAGGAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTATATTTATATAATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTAt                                                                                                                                              >  1:1503729/1‑150 (MQ=255)
      atatatGGAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTAtct                                                                                                                                            <  2:441439/150‑1 (MQ=255)
       tatatGGAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTAtctc                                                                                                                                           >  1:898017/1‑150 (MQ=255)
        atatGGAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATctct                                                                                                                                          <  1:2583269/150‑1 (MQ=255)
        atatGGAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATctct                                                                                                                                          <  1:4526788/150‑1 (MQ=255)
         tatGGAGAATCAAACATACTAACGACTATTAATAACATAAAAGACAATATATTTAAAAAAATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCtt                                                                                                                                         <  2:891064/150‑1 (MQ=255)
           tGGAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCtttt                                                                                                                                       <  1:160376/150‑1 (MQ=255)
            ggAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCttttt                                                                                                                                      <  1:3977165/150‑1 (MQ=255)
            ggAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCttttt                                                                                                                                      <  1:2649386/150‑1 (MQ=255)
            ggAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCttttt                                                                                                                                      >  2:532691/1‑150 (MQ=255)
                   cAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCAGTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCGTTATTTGTAGTATCTCTTTTTGGAatat                                                                                                                               >  1:4356625/1‑150 (MQ=255)
                          aCTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAAtt                                                                                                                        <  2:2396227/150‑1 (MQ=255)
                          aCTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAAtt                                                                                                                        <  2:4512933/150‑1 (MQ=255)
                              aCGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTAt                                                                                                                    >  1:3186062/1‑150 (MQ=255)
                              aCGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTAt                                                                                                                    <  1:978699/150‑1 (MQ=255)
                               cGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAGTTTTagg                                                                                                                   >  2:1355302/1‑148 (MQ=255)
                                   tATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTc                                                                                                               >  1:1645578/1‑150 (MQ=255)
                                    aTTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCa                                                                                                              >  1:3583835/1‑150 (MQ=255)
                                     ttACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCAt                                                                                                             >  1:431693/1‑150 (MQ=255)
                                      tACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCAtt                                                                                                            >  1:555421/1‑150 (MQ=255)
                                       aCTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCAttt                                                                                                           >  2:165380/1‑150 (MQ=255)
                                         tAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCAttttt                                                                                                         <  2:1455803/150‑1 (MQ=255)
                                           aCATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCg                                                                                                       <  2:2500882/150‑1 (MQ=255)
                                           aCATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCg                                                                                                       <  1:3816455/150‑1 (MQ=255)
                                               aaaaGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGa                                                                                                   <  2:2081285/150‑1 (MQ=255)
                                                 aaGACAATATATTTAAAAACATTATATTAATAACAATGTTATAAATTTTTATTGATTCTATATTTATTAGAAATAATAAAATAAATGCAACTTTATTAGTAGTATATCTTTTTGGAATATCTCAATTTTAAGTGTCATTTTTCGGGGAAg                                                                                                 >  2:671467/1‑150 (MQ=255)
                                                  aGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAgg                                                                                                >  1:1316369/1‑150 (MQ=255)
                                                  aGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAgg                                                                                                >  1:2916240/1‑150 (MQ=255)
                                                    aCAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGat                                                                                              >  1:2888913/1‑150 (MQ=255)
                                                     cAATATATTTAAAAACATTAGATTTATACCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGata                                                                                             <  2:4190485/150‑1 (MQ=255)
                                                      aaTATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGatat                                                                                            <  2:4046278/150‑1 (MQ=255)
                                                           atTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAgaga                                                                                       <  1:1973854/150‑1 (MQ=255)
                                                            tttAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGAt                                                                                      >  1:3558551/1‑150 (MQ=255)
                                                            tttAAAAACATTAGATTTATATCATAGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGAt                                                                                      >  1:226179/1‑150 (MQ=255)
                                                                aaaaCATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTaa                                                                                  >  2:3518156/1‑150 (MQ=255)
                                                                aaaaCATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTaa                                                                                  <  2:4503873/150‑1 (MQ=255)
                                                                  aaCATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTaagc                                                                                >  2:2045464/1‑150 (MQ=255)
                                                                   aCATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTaagca                                                                               <  1:1537677/150‑1 (MQ=255)
                                                                     aTTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTaagcaag                                                                             <  1:795945/150‑1 (MQ=255)
                                                                         gATTTATATCATTGTTATTATTTTTTATTGGTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCatt                                                                         >  1:3889805/1‑150 (MQ=255)
                                                                         gATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCatt                                                                         >  1:44409/1‑150 (MQ=255)
                                                                          aTTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCattt                                                                        <  2:868329/150‑1 (MQ=255)
                                                                          aTTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCattt                                                                        <  1:173767/150‑1 (MQ=255)
                                                                           tttATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCattta                                                                       >  2:4548507/1‑150 (MQ=255)
                                                                            ttATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCatttat                                                                      <  2:4332676/150‑1 (MQ=255)
                                                                             tataTCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTGGTAGTATCTCTTTTTGGAATATCTCAATTTTCTGTGTCATTTTTCGGGGACGGATATAGAGATTTAAGCAAGCatgtatt                                                                     >  2:2360862/1‑150 (MQ=255)
                                                                                  cATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGaa                                                                >  2:4093630/1‑150 (MQ=255)
                                                                                    ttGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATg                                                              <  2:3827723/150‑1 (MQ=255)
                                                                                     tGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGt                                                             >  1:425850/1‑150 (MQ=255)
                                                                                      gTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTa                                                            <  2:1231174/150‑1 (MQ=255)
                                                                                       ttattaTTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTAt                                                           <  1:619396/150‑1 (MQ=255)
                                                                                       ttattaTTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTAt                                                           <  1:409516/150‑1 (MQ=255)
                                                                                       ttattaTTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTAt                                                           >  1:3169386/1‑150 (MQ=255)
                                                                                         attattTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTAttt                                                         <  1:1339152/150‑1 (MQ=255)
                                                                                           tattTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTAttttt                                                       <  2:1928215/150‑1 (MQ=255)
                                                                                            attTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTttc                                                      <  1:988240/150‑1 (MQ=255)
                                                                                            attTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTttc                                                      <  2:1035318/150‑1 (MQ=255)
                                                                                             ttttttATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTttcg                                                     <  1:1076625/150‑1 (MQ=255)
                                                                                                  tATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGa                                                >  2:2324792/1‑150 (MQ=255)
                                                                                                    ttGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGAcc                                              >  2:3957798/1‑150 (MQ=255)
                                                                                                     tGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCt                                             <  1:275928/150‑1 (MQ=255)
                                                                                                     tGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCt                                             >  1:3324954/1‑150 (MQ=255)
                                                                                                     tGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCt                                             >  1:1205319/1‑150 (MQ=255)
                                                                                                        ttCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTg                                          >  2:4580632/1‑150 (MQ=255)
                                                                                                         tCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGc                                         <  1:4566894/150‑1 (MQ=255)
                                                                                                          cTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCt                                        <  1:177683/150‑1 (MQ=255)
                                                                                                                ttattaGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACa                                  >  2:1007434/1‑150 (MQ=255)
                                                                                                                  attaGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATa                                >  2:270708/1‑150 (MQ=255)
                                                                                                                   ttaGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATaa                               <  2:608558/150‑1 (MQ=255)
                                                                                                                   ttaGAAATAATAAAATAAAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATaa                               <  2:3583835/150‑1 (MQ=255)
                                                                                                                        aataatAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTc                          >  1:120638/1‑150 (MQ=255)
                                                                                                                        aataatAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTc                          >  1:2374652/1‑150 (MQ=255)
                                                                                                                           aataaaataaAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtt                       >  1:1083103/1‑150 (MQ=255)
                                                                                                                           aataaaataaAGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtt                       >  1:1726485/1‑150 (MQ=255)
                                                                                                                             taaaataaaGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtttt                     <  1:2454410/150‑1 (MQ=255)
                                                                                                                              aaaataaaGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGACGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttt                    >  1:1830742/1‑150 (MQ=255)
                                                                                                                               aaataaaGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtttttt                   <  1:515142/150‑1 (MQ=255)
                                                                                                                               aaataaaGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtttttt                   <  2:3237995/150‑1 (MQ=255)
                                                                                                                                 ataaaGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTa                 <  2:2599578/150‑1 (MQ=255)
                                                                                                                                   aaaGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATACCAGTCGTTTTTTTAAt               >  2:4428377/1‑150 (MQ=255)
                                                                                                                                   aaaGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAt               >  2:3167611/1‑150 (MQ=255)
                                                                                                                                   aaaGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAt               >  2:3753766/1‑150 (MQ=255)
                                                                                                                                   aaaGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAt               >  2:2027091/1‑150 (MQ=255)
                                                                                                                                    aaGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTatat              >  1:1215205/1‑147 (MQ=255)
                                                                                                                                    aaGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAtt              <  2:3623456/150‑1 (MQ=255)
                                                                                                                                    aaGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAtt              <  2:3623595/150‑1 (MQ=255)
                                                                                                                                     aGGCATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAttt             >  2:3799894/1‑150 (MQ=255)
                                                                                                                                        cATCTTTATTTGAAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTtata          >  2:1171238/1‑150 (MQ=255)
                                                                                                                                         aTCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATaa         <  2:3309094/150‑1 (MQ=255)
                                                                                                                                           cTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATaaaa       >  2:2204011/1‑150 (MQ=255)
                                                                                                                                           cTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATaaaa       >  2:1392308/1‑150 (MQ=255)
                                                                                                                                              tATTTGAAGTATCTCTTTTTGGAATATCTCAAGTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTTTTAaataa    >  1:1981678/1‑150 (MQ=255)
                                                                                                                                               aTTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAaataat   >  1:634258/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CACGTAATATATGGAGAATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATT  >  CP009273/2463612‑2463905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑