BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,472,897	A→T	41.9%	T73S (ACT→TCT)	dsdA →	D‑serine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,472,897	0	A	T	41.9%	164.7 / inf	265	T73S (ACT→TCT)	dsdA	D‑serine dehydratase
Reads supporting (aligned to +/- strand): ref base A (71/83); new base T (53/58); total (124/141)
Fisher's exact test for biased strand distribution p-value = 8.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

AAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGG  >  CP009273/2472748‑2473046

   aaaCCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCCTGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTg                                                                                                                                                    >  2:2513387/1‑150 (MQ=255)

     accaccTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTggg                                                                                                                                                  >  2:1060804/1‑150 (MQ=255)

      ccaccTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTgggg                                                                                                                                                 <  2:4181779/150‑1 (MQ=255)

      ccaccTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTgggg                                                                                                                                                 >  2:4267394/1‑150 (MQ=255)

      ccaccTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTgggg                                                                                                                                                 <  2:382801/150‑1 (MQ=255)

            ggTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGatta                                                                                                                                           >  2:3588443/1‑150 (MQ=255)

               ttAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTg                                                                                                                                        >  2:2594251/1‑150 (MQ=255)

                tAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGa                                                                                                                                       >  2:225788/1‑150 (MQ=255)

                 aaTCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGaa                                                                                                                                      <  2:3339531/150‑1 (MQ=255)

                      tGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGa                                                                                                                                 <  1:435701/150‑1 (MQ=255)

                        gCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAAc                                                                                                                               >  1:4151803/1‑150 (MQ=255)

                        gCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAAc                                                                                                                               >  1:684506/1‑150 (MQ=255)

                         cACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACt                                                                                                                              <  1:935897/150‑1 (MQ=255)

                           cGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTgg                                                                                                                            >  1:3777224/1‑150 (MQ=255)

                           cGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTgg                                                                                                                            >  1:3287378/1‑150 (MQ=255)

                            gACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGt                                                                                                                           >  1:2035661/1‑150 (MQ=255)

                             aCCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGtt                                                                                                                          <  1:550425/150‑1 (MQ=255)

                              ccTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTg                                                                                                                         >  2:3676642/1‑150 (MQ=255)

                               cTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGc                                                                                                                        <  1:3471524/150‑1 (MQ=255)

                                   ttGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCAtt                                                                                                                    <  1:2784087/150‑1 (MQ=255)

                                   ttGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCAtt                                                                                                                    >  1:2563736/1‑150 (MQ=255)

                                       cTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAg                                                                                                                >  2:3208955/1‑150 (MQ=255)

                                        tGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGc                                                                                                               >  2:2027651/1‑150 (MQ=255)

                                         gAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCt                                                                                                              <  1:3043853/150‑1 (MQ=255)

                                          aaGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTa                                                                                                             <  2:1012514/150‑1 (MQ=255)

                                          aaGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTa                                                                                                             >  2:3147241/1‑150 (MQ=255)

                                            ggTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCCGCTATg                                                                                                           <  1:3155896/150‑1 (MQ=255)

                                            ggTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATg                                                                                                           <  2:88861/150‑1 (MQ=255)

                                                   cTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAAc                                                                                                    <  2:1622925/150‑1 (MQ=255)

                                                           ggCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGaa                                                                                            <  1:2252038/150‑1 (MQ=255)

                                                              cTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGaaaaa                                                                                         >  1:3833069/1‑150 (MQ=255)

                                                               tGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAg                                                                                        >  2:2448710/1‑150 (MQ=255)

                                                               tGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAg                                                                                        >  2:658488/1‑150 (MQ=255)

                                                                 aCCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGaa                                                                                      >  1:1510486/1‑150 (MQ=255)

                                                                 aCCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGaa                                                                                      >  2:2143025/1‑150 (MQ=255)

                                                                 aCCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGaa                                                                                      >  2:1598141/1‑150 (MQ=255)

                                                                    gAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAatat                                                                                   >  2:2640540/1‑150 (MQ=255)

                                                                    gAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAatat                                                                                   >  2:3936983/1‑150 (MQ=255)

                                                                    gAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAatat                                                                                   >  2:3165457/1‑150 (MQ=255)

                                                                       cAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAg                                                                                <  2:4440092/150‑1 (MQ=255)

                                                                       cAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAg                                                                                >  1:1080653/1‑150 (MQ=255)

                                                                        aGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAATGAATATCAGc                                                                               >  1:704174/1‑150 (MQ=255)

                                                                        aGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGc                                                                               >  1:2846784/1‑150 (MQ=255)

                                                                         ggATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCa                                                                              <  1:982283/150‑1 (MQ=255)

                                                                           aTGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAAc                                                                            <  2:2075900/150‑1 (MQ=255)

                                                                            tGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAAcc                                                                           <  2:3012107/150‑1 (MQ=255)

                                                                            tGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAAcc                                                                           <  1:1764651/150‑1 (MQ=255)

                                                                              ttCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGa                                                                         <  1:1365690/150‑1 (MQ=255)

                                                                                cAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATc                                                                       <  2:1089372/150‑1 (MQ=255)

                                                                                cAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATc                                                                       <  2:1898450/150‑1 (MQ=255)

                                                                                 aGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCa                                                                      >  2:3875242/1‑150 (MQ=255)

                                                                                  ggACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAg                                                                     >  1:4578978/1‑150 (MQ=255)

                                                                                   gACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCGAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGc                                                                    <  1:2664945/150‑1 (MQ=255)

                                                                                   gACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGc                                                                    <  1:132743/150‑1 (MQ=255)

                                                                                      gCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCggg                                                                 <  2:1423051/150‑1 (MQ=255)

                                                                                           tGCGCGCTTATCCCATTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACt                                                            >  2:1032930/1‑150 (MQ=255)

                                                                                              gcgcTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGtt                                                         <  2:1835620/150‑1 (MQ=255)

                                                                                               cgcTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTa                                                        >  2:4221035/1‑150 (MQ=255)

                                                                                                 cTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACt                                                      <  2:2273727/150‑1 (MQ=255)

                                                                                                  ttATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTg                                                     <  2:527423/150‑1 (MQ=255)

                                                                                                    aTCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGaa                                                   <  2:4497924/150‑1 (MQ=255)

                                                                                                     tCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGaaa                                                  <  2:2803864/150‑1 (MQ=255)

                                                                                                       ccGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGaaaaa                                                >  1:4414412/1‑150 (MQ=255)

                                                                                                        cGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGaaaaaa                                               <  1:2363786/150‑1 (MQ=255)

                                                                                                          ttttGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGa                                             <  1:4461922/150‑1 (MQ=255)

                                                                                                           tttGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGAt                                            <  2:322135/150‑1 (MQ=255)

                                                                                                            ttGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATa                                           >  2:2917715/1‑150 (MQ=255)

                                                                                                            ttGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATa                                           <  1:355473/150‑1 (MQ=255)

                                                                                                             tGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAg                                          <  2:3499840/150‑1 (MQ=255)

                                                                                                               cACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGcc                                        <  1:1027508/150‑1 (MQ=255)

                                                                                                                aCCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCa                                       <  1:3877109/150‑1 (MQ=255)

                                                                                                                 cccTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCAt                                      <  1:4367382/150‑1 (MQ=255)

                                                                                                                  ccTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCAtt                                     <  2:1911081/150‑1 (MQ=255)

                                                                                                                    tATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTg                                   <  2:4128886/150‑1 (MQ=255)

                                                                                                                     aTCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGc                                  >  1:2125773/1‑150 (MQ=255)

                                                                                                                      tCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGcc                                 <  2:458754/150‑1 (MQ=255)

                                                                                                                       cTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGccc                                >  1:4247520/1‑150 (MQ=255)

                                                                                                                          gCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCAtt                             <  1:3198939/150‑1 (MQ=255)

                                                                                                                             aaaGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTcc                          <  1:219534/150‑1 (MQ=255)

                                                                                                                             aaaGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTcc                          <  1:1812269/150‑1 (MQ=255)

                                                                                                                              aaGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCg                         >  1:3483306/1‑150 (MQ=255)

                                                                                                                              aaGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCg                         >  1:355137/1‑150 (MQ=255)

                                                                                                                                gCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGc                       >  2:1415492/1‑150 (MQ=255)

                                                                                                                                gCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGc                       >  2:3735651/1‑150 (MQ=255)

                                                                                                                                  aTTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTc                     >  1:3992384/1‑150 (MQ=255)

                                                                                                                                   tttCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAATAGAATATCAGCAACCGATCAGCGGGAAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTca                    >  2:2711556/1‑149 (MQ=255)

                                                                                                                                   tttCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTcc                    <  2:2882895/150‑1 (MQ=255)

                                                                                                                                      ccTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATa                 <  2:564476/150‑1 (MQ=255)

                                                                                                                                      ccTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATa                 >  2:2335780/1‑150 (MQ=255)

                                                                                                                                        tGAAACTGCTGCCTCTGGGGGGATTATTGAATAAGAAATGGTTGCCATTCCAGCTATGAAAAAAAGGCTGGAAAAAGAATATAAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATATGACCATTTCCGGCTCAATaaa               >  1:1891574/1‑150 (MQ=255)

                                                                                                                                        tGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATaaa               >  1:1684322/1‑150 (MQ=255)

                                                                                                                                         gAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATaaaa              <  1:802540/150‑1 (MQ=255)

                                                                                                                                           aaCTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATCTCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGc            <  2:1134320/150‑1 (MQ=255)

                                                                                                                                           aaCTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGc            <  1:1183749/150‑1 (MQ=255)

                                                                                                                                            aCTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCa           >  2:1398424/1‑150 (MQ=255)

                                                                                                                                             ctgctgCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCAc          <  1:1655977/150‑1 (MQ=255)

                                                                                                                                                ctgcCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCAcgcg       <  1:4527022/150‑1 (MQ=255)

                                                                                                                                                 tgcCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACgcgg      <  2:2896405/150‑1 (MQ=255)

                                                                                                                                                    cACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGcggcgg   >  2:3482582/1‑150 (MQ=255)

                                                                                                                                                     aCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCggg  >  1:2749967/1‑150 (MQ=255)

AAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGG  >  CP009273/2472748‑2473046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`