Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,487,835 G→T 11.2% S16Y (TCT→TAT)  yfdX ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,487,8350GT11.2% 1029.5 / 68.3 292S16Y (TCT→TAT) yfdXuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (126/133);  new base T (17/16);  total (143/149)
Fisher's exact test for biased strand distribution p-value = 8.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 293 total aligned reads displayed.

AGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTA  >  CP009273/2487688‑2487984
                                                                                                                                                   |                                                                                                                                                     
aGGCGCGCTACCTGCACGTCTCGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCTCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGAt                                                                                                                                                     <  2:346681/150‑1 (MQ=255)
aGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGAt                                                                                                                                                     >  2:2400184/1‑150 (MQ=255)
aGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGAt                                                                                                                                                     >  2:1898616/1‑150 (MQ=255)
aGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGAt                                                                                                                                                     >  1:4316930/1‑150 (MQ=255)
aGGCGAGCTACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGAt                                                                                                                                                     >  2:4025845/1‑150 (MQ=255)
 ggCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATg                                                                                                                                                    >  1:1692445/1‑150 (MQ=255)
   agcgcTAACTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTATTGGTTTTCTGAACTTGCTGAGTTTGATTTTGTGACGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAATATgac                                                                                                                                                  >  1:811053/2‑148 (MQ=255)
        tACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAAt                                                                                                                                             <  2:1105759/150‑1 (MQ=255)
        tACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAAt                                                                                                                                             <  1:2919489/150‑1 (MQ=255)
         aCCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAAtt                                                                                                                                            <  1:2118829/150‑1 (MQ=255)
          ccTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTg                                                                                                                                           <  1:3716714/150‑1 (MQ=255)
            tGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCt                                                                                                                                         >  2:1765815/1‑150 (MQ=255)
            tGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCt                                                                                                                                         <  1:11235/150‑1 (MQ=255)
            tGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGGTGAAGATGCCAGAATTGCt                                                                                                                                         >  1:3084661/1‑150 (MQ=255)
             gCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTGTCAGCAGCCCATACAGTTGAATATGCCAGAATTGCtg                                                                                                                                        <  1:1493370/150‑1 (MQ=255)
              cACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCtgt                                                                                                                                       <  2:896393/150‑1 (MQ=255)
              cACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCtgt                                                                                                                                       <  1:1011159/150‑1 (MQ=255)
                 gTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGAc                                                                                                                                    >  2:1692246/1‑150 (MQ=255)
                 gTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGAc                                                                                                                                    <  1:3176539/150‑1 (MQ=255)
                  tCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGAcc                                                                                                                                   >  1:2492947/1‑150 (MQ=255)
                   cACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCa                                                                                                                                  >  2:1790397/1‑150 (MQ=255)
                         tcgcatATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTgg                                                                                                                            >  2:2686599/1‑150 (MQ=255)
                          cgcatATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGc                                                                                                                           >  2:436513/1‑150 (MQ=255)
                           gcatATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAATATGCCAGAATTGCTGTGACCATCGTGGcc                                                                                                                          <  1:1980266/150‑1 (MQ=255)
                           gcatATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGcc                                                                                                                          >  2:2936687/1‑150 (MQ=255)
                            catATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCa                                                                                                                         >  1:3972113/1‑150 (MQ=255)
                             atatAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCAt                                                                                                                        >  1:1306021/1‑150 (MQ=255)
                              tataAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAATATGCCAGAATTGCTGTGACCATCGTGGCCATa                                                                                                                       >  1:2390425/1‑150 (MQ=255)
                              tataAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAATATGCCAGAATTGCTGTGACCATCGTGGCCATa                                                                                                                       >  1:333962/1‑150 (MQ=255)
                               ataAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATaa                                                                                                                      >  2:4421267/1‑150 (MQ=255)
                                 aaaCCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAAtt                                                                                                                    >  1:4121237/1‑150 (MQ=255)
                                  aaCCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTa                                                                                                                   <  2:3852995/150‑1 (MQ=255)
                                           cAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCa                                                                                                          >  1:1539604/1‑150 (MQ=255)
                                           cAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCa                                                                                                          <  2:1523057/150‑1 (MQ=255)
                                           cAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCa                                                                                                          >  2:3391949/1‑150 (MQ=255)
                                            agagATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCAt                                                                                                         >  2:1293900/1‑150 (MQ=255)
                                            agagATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCAt                                                                                                         <  2:955124/150‑1 (MQ=255)
                                             gagaTACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATa                                                                                                        <  1:4577682/150‑1 (MQ=255)
                                             gagaTACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATa                                                                                                        <  1:829338/150‑1 (MQ=255)
                                               gaTACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACt                                                                                                      <  1:2213925/150‑1 (MQ=255)
                                                aTACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACtt                                                                                                     <  2:1375411/150‑1 (MQ=255)
                                                aTACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACtt                                                                                                     <  1:4435595/150‑1 (MQ=255)
                                                 tACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTa                                                                                                    <  1:4010323/150‑1 (MQ=255)
                                                 tACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCAACGTGGCCATAATTAAACGTTTCATACTTa                                                                                                    >  1:3612941/1‑150 (MQ=255)
                                                   cGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTAcc                                                                                                  <  2:368597/150‑1 (MQ=255)
                                                    gCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCt                                                                                                 <  2:3513045/150‑1 (MQ=255)
                                                     ctctGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAATATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTc                                                                                                <  2:1236809/150‑1 (MQ=255)
                                                     ctctGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTc                                                                                                <  2:2215837/150‑1 (MQ=255)
                                                      tctGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCa                                                                                               <  1:2845094/150‑1 (MQ=255)
                                                          ctgccgctgccgTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCt                                                                                           <  2:3079142/150‑1 (MQ=255)
                                                          ctgccgctgccgTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCt                                                                                           <  1:4634358/150‑1 (MQ=255)
                                                           tgccgctgccgTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAATATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCtt                                                                                          <  2:1042057/150‑1 (MQ=255)
                                                           tgccgctgccgTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCtt                                                                                          <  2:3213683/150‑1 (MQ=255)
                                                           tgccgctgccgTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCtt                                                                                          >  1:3465394/1‑150 (MQ=255)
                                                               gctgccgTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAAc                                                                                      >  1:2019913/1‑150 (MQ=255)
                                                                ctgccgTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACa                                                                                     >  2:2841729/1‑150 (MQ=255)
                                                                     gTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGtttt                                                                                >  1:3759231/1‑150 (MQ=255)
                                                                          cTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAATATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGaaa                                                                           <  2:859914/150‑1 (MQ=255)
                                                                          cTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGaaa                                                                           <  1:626651/150‑1 (MQ=255)
                                                                            gggTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAAc                                                                         <  2:558798/150‑1 (MQ=255)
                                                                            gggTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAAc                                                                         >  2:3097734/1‑150 (MQ=255)
                                                                             ggTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAATATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAAcg                                                                        <  1:1253812/150‑1 (MQ=255)
                                                                              gTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAAcgc                                                                       >  1:2863461/1‑150 (MQ=255)
                                                                              gTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAAcgc                                                                       >  2:4468732/1‑150 (MQ=255)
                                                                                 tGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAAcgccgc                                                                    >  1:4426403/1‑150 (MQ=255)
                                                                                    tGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCcat                                                                 <  2:3000936/150‑1 (MQ=255)
                                                                                       aCTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAATATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTCACCTCATTCTTTAACAGTTTTAGAAAACGCCGCcatcat                                                              <  1:2531307/150‑1 (MQ=255)
                                                                                        cTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCata                                                             <  2:4593092/150‑1 (MQ=255)
                                                                                         ttGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCatat                                                            <  2:772710/150‑1 (MQ=255)
                                                                                          tGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATAtt                                                           <  2:4145033/150‑1 (MQ=255)
                                                                                          tGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATAtt                                                           <  2:633641/150‑1 (MQ=255)
                                                                                            cTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTgg                                                         >  1:4463548/1‑150 (MQ=255)
                                                                                               aGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTg                                                      >  1:725430/1‑150 (MQ=255)
                                                                                                   tGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCa                                                  >  1:3880842/1‑150 (MQ=255)
                                                                                                       gttgtGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGaa                                              >  1:1081643/1‑150 (MQ=255)
                                                                                                       gttgtGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGaa                                              >  1:566081/1‑150 (MQ=255)
                                                                                                          gtgCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATg                                           <  1:2807808/150‑1 (MQ=255)
                                                                                                              cGCTACCGGAGCATTATCAGCAGTCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGaa                                       >  1:4441357/1‑150 (MQ=255)
                                                                                                                  aCCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCAGATTGGGTGAGCATGAAATGAGAAATCa                                   >  2:4299813/1‑150 (MQ=255)
                                                                                                                     ggAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCagag                                <  1:1657645/150‑1 (MQ=255)
                                                                                                                      gAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCagaga                               <  2:3972113/150‑1 (MQ=255)
                                                                                                                      gAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCagaga                               <  2:1649649/150‑1 (MQ=255)
                                                                                                                      gAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCagaga                               <  1:4375173/150‑1 (MQ=255)
                                                                                                                      gAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCagaga                               <  1:2329869/150‑1 (MQ=255)
                                                                                                                      gAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCagaga                               >  1:4073135/1‑150 (MQ=255)
                                                                                                                        gCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGc                             >  2:3620922/1‑150 (MQ=255)
                                                                                                                        gCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGc                             <  1:1944921/150‑1 (MQ=255)
                                                                                                                         cATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCa                            <  1:1378334/150‑1 (MQ=255)
                                                                                                                          aTTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCaa                           >  1:2741650/1‑150 (MQ=255)
                                                                                                                                agcagcCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCa                     <  2:2571473/150‑1 (MQ=255)
                                                                                                                                agcagcCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCa                     >  1:3677928/1‑150 (MQ=255)
                                                                                                                                  cagcCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCAtt                   <  1:4022327/150‑1 (MQ=255)
                                                                                                                                  cagcCCATACAGTTGAAGATGACAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCAtt                   <  2:1555355/150‑1 (MQ=255)
                                                                                                                                      ccATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGa               <  1:2247951/150‑1 (MQ=255)
                                                                                                                                       cATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGaa              >  1:917837/1‑150 (MQ=255)
                                                                                                                                        aTACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGaaa             <  2:4086789/150‑1 (MQ=255)
                                                                                                                                             gTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGtt        <  2:2785216/150‑1 (MQ=255)
                                                                                                                                              ttGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTa       >  2:295486/1‑150 (MQ=255)
                                                                                                                                                gAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATc     >  2:3171602/1‑150 (MQ=255)
                                                                                                                                                  aGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCtt   <  2:3913074/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTA  >  CP009273/2487688‑2487984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑