Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,545,086 C→A 46.5% D138Y (GAT→TAT)  ypeA ← putative acyl‑CoA transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,545,0860CA46.5% 166.6 / inf 245D138Y (GAT→TAT) ypeAputative acyl‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base C (63/68);  new base A (51/62);  total (114/131)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

Only 100 of 253 total aligned reads displayed.

CAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCAGCCGATTAA  >  CP009273/2544941‑2545235
                                                                                                                                                 |                                                                                                                                                     
cAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACtcttcatattca                                                                                                                                                   <  2:3228158/150‑1 (MQ=255)
 aGTACCCTTCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTtcaa                                                                                                                                                  >  2:3273967/1‑150 (MQ=255)
 aGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATtcaa                                                                                                                                                  >  1:3590455/1‑150 (MQ=255)
  gTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTtcaat                                                                                                                                                 <  1:2489647/150‑1 (MQ=255)
    aCCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAATCGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATtcaatca                                                                                                                                               <  1:214990/150‑1 (MQ=255)
    aCCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGATACTCAGTACTCTTCATATtcaatca                                                                                                                                               <  1:39885/150‑1 (MQ=255)
        tGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACg                                                                                                                                           >  2:3173463/1‑150 (MQ=255)
         gCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGc                                                                                                                                          >  2:225489/1‑150 (MQ=255)
         gCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGc                                                                                                                                          >  2:4304667/1‑150 (MQ=255)
          cTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCt                                                                                                                                         >  1:1016940/1‑150 (MQ=255)
           tCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCtt                                                                                                                                        <  1:2446517/150‑1 (MQ=255)
             gcgcgACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTAc                                                                                                                                      <  2:1606075/150‑1 (MQ=255)
             gcgcgACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTAc                                                                                                                                      >  2:3028797/1‑150 (MQ=255)
             gcgcgACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTAc                                                                                                                                      >  1:1956211/1‑150 (MQ=255)
                    gCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACt                                                                                                                               >  1:799338/1‑150 (MQ=255)
                    gCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACt                                                                                                                               >  1:546373/1‑150 (MQ=255)
                     cACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTc                                                                                                                              >  1:2891996/1‑150 (MQ=255)
                      aCCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCa                                                                                                                             <  2:2172596/150‑1 (MQ=255)
                      aCCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCa                                                                                                                             >  1:655654/1‑150 (MQ=255)
                        cGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGc                                                                                                                           <  2:1907381/150‑1 (MQ=255)
                         ggCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCa                                                                                                                          <  1:3941907/150‑1 (MQ=255)
                          gCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCAc                                                                                                                         <  2:3572370/150‑1 (MQ=255)
                           cAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACg                                                                                                                        <  2:2704801/150‑1 (MQ=255)
                              tGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCg                                                                                                                     <  1:1797359/150‑1 (MQ=255)
                                aCAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGc                                                                                                                   <  1:2632218/150‑1 (MQ=255)
                                aCAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGc                                                                                                                   >  1:504109/1‑150 (MQ=255)
                                      aGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTc                                                                                                             <  1:1221644/150‑1 (MQ=255)
                                       gCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCa                                                                                                            <  1:1400098/150‑1 (MQ=255)
                                         aCCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCata                                                                                                          <  1:3660881/150‑1 (MQ=255)
                                          cccAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCatat                                                                                                         <  2:1216103/150‑1 (MQ=255)
                                          cccAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCatat                                                                                                         <  2:3092586/150‑1 (MQ=255)
                                          cccAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCatat                                                                                                         <  1:882245/150‑1 (MQ=255)
                                           ccAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATc                                                                                                        >  1:834289/1‑150 (MQ=255)
                                               gTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAg                                                                                                    >  2:2504999/1‑150 (MQ=255)
                                                ttCGCGCCTTAAGCAATAGCACCATCCAGTAGAGCAATGGTAAACGCAGTCGACCGTGCTCTTCATATTGGACATTATGAAACTCATTACTCTTCATATTCAATCAAACGCTTACCCAGACTCTGCACGTCGGCGTGTTCATATCCCAgg                                                                                                   <  2:465411/150‑1 (MQ=255)
                                                 tCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGc                                                                                                  <  1:2691326/150‑1 (MQ=255)
                                                  cgcgCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCg                                                                                                 <  1:1914676/150‑1 (MQ=255)
                                                    cgcCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGtt                                                                                               <  1:4178597/150‑1 (MQ=255)
                                                      ccTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCa                                                                                             <  1:3763029/150‑1 (MQ=255)
                                                       cTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCAt                                                                                            <  2:291210/150‑1 (MQ=255)
                                                       cTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCAt                                                                                            >  2:2035821/1‑150 (MQ=255)
                                                           aGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACAt                                                                                        >  2:561740/1‑150 (MQ=255)
                                                            gCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACAtt                                                                                       <  2:3110705/150‑1 (MQ=255)
                                                            gCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACAtt                                                                                       <  2:3670415/150‑1 (MQ=255)
                                                               aTAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCg                                                                                    >  1:1614267/1‑150 (MQ=255)
                                                               aTAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCg                                                                                    <  1:610170/150‑1 (MQ=255)
                                                                 aGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAg                                                                                  <  2:3422069/150‑1 (MQ=255)
                                                                  gCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGc                                                                                 >  2:3409205/1‑150 (MQ=255)
                                                                   cacaAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCCGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCa                                                                                <  1:3087002/150‑1 (MQ=255)
                                                                     caAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCAcc                                                                              <  2:2595103/150‑1 (MQ=255)
                                                                        gCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATg                                                                           <  2:1971753/150‑1 (MQ=255)
                                                                          cAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCTTCATAATGGACAGGATGAATGTCAGTACTCTTCATTTTCAATCTATAGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGAGTTCATTCATTCCGAGCACCATGTc                                                                         <  2:3824885/150‑1 (MQ=255)
                                                                          cAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTc                                                                         <  2:3824841/150‑1 (MQ=255)
                                                                              agagCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTa                                                                     <  2:178892/150‑1 (MQ=255)
                                                                               gagCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTAt                                                                    <  1:1125492/150‑1 (MQ=255)
                                                                                agCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATc                                                                   <  1:284057/150‑1 (MQ=255)
                                                                                  cAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCtt                                                                 >  2:4558807/1‑150 (MQ=255)
                                                                                    aGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTcc                                                               >  1:4348557/1‑150 (MQ=255)
                                                                                     gggTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCg                                                              <  2:874161/150‑1 (MQ=255)
                                                                                          aaCGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGcaca                                                         >  1:1892780/1‑150 (MQ=255)
                                                                                          aaCGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGcaca                                                         <  1:2939651/150‑1 (MQ=255)
                                                                                           aCGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACAt                                                        >  1:2937731/1‑150 (MQ=255)
                                                                                             gCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTg                                                      >  1:722664/1‑150 (MQ=255)
                                                                                                ggCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATc                                                   <  2:4048014/150‑1 (MQ=255)
                                                                                                      cGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAAtt                                             <  1:3440378/150‑1 (MQ=255)
                                                                                                        tGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCGTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAAtttt                                           <  2:1347055/150‑1 (MQ=255)
                                                                                                        tGCGCATAATAATGGACAGGATGAAACTCAGTAATCTTCATCTTCAAACAAACGCTAACCCAGACTCAGCACGACGGCGTGTTCATATACCAGGCGTTAATACATTACGAGCACCATGTCGTTATCTTCCGCCACATAGATCTGAAattt                                           >  1:2738108/1‑150 (MQ=255)
                                                                                                         gcgcATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTc                                          <  2:802467/150‑1 (MQ=255)
                                                                                                          cgcATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCg                                         >  2:1758515/1‑150 (MQ=255)
                                                                                                           gcATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCgg                                        <  2:776903/150‑1 (MQ=255)
                                                                                                             atcatAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGc                                      >  2:1062940/1‑150 (MQ=255)
                                                                                                              tcatAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCa                                     <  1:3699436/150‑1 (MQ=255)
                                                                                                               catAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAg                                    >  1:3869059/1‑150 (MQ=255)
                                                                                                                 tAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGcc                                  >  2:3339151/1‑150 (MQ=255)
                                                                                                                 tAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGAGTTCATACATTACGAGCACCATTTCGTTATCTTCCGTAACATTGATCTGAATTTTCGGGCAgac                                  >  2:2041316/1‑148 (MQ=255)
                                                                                                                  aaTGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCa                                 <  1:212185/150‑1 (MQ=255)
                                                                                                                  aaTGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCa                                 >  2:3292586/1‑150 (MQ=255)
                                                                                                                   aTGGACAGGATGAAACTCCGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCAc                                <  1:102069/150‑1 (MQ=255)
                                                                                                                    tGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACg                               <  1:162722/150‑1 (MQ=255)
                                                                                                                     ggACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGa                              >  1:3403078/1‑150 (MQ=255)
                                                                                                                       aCAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGc                            >  2:3466483/1‑150 (MQ=255)
                                                                                                                       aCAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGc                            >  2:3724712/1‑150 (MQ=255)
                                                                                                                        cAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCa                           <  2:3966048/150‑1 (MQ=255)
                                                                                                                        cAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCa                           <  1:73371/150‑1 (MQ=255)
                                                                                                                         aGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCaa                          <  2:4606687/150‑1 (MQ=255)
                                                                                                                         aGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCaa                          <  2:4090418/150‑1 (MQ=255)
                                                                                                                          ggATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAAt                         <  2:4541526/150‑1 (MQ=255)
                                                                                                                           gATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATc                        >  1:3251479/1‑150 (MQ=255)
                                                                                                                           gATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATc                        <  1:3636141/150‑1 (MQ=255)
                                                                                                                           gATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATc                        <  1:1092874/150‑1 (MQ=255)
                                                                                                                            aTGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCa                       >  1:1699386/1‑150 (MQ=255)
                                                                                                                               aaaCTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGcc                    <  1:2231364/150‑2 (MQ=255)
                                                                                                                               aaaCTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCt                    >  1:2976747/1‑150 (MQ=255)
                                                                                                                                aaCTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTCTCTACCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCtt                   >  2:1103376/1‑150 (MQ=255)
                                                                                                                                 aCTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCttt                  >  1:2739469/1‑150 (MQ=255)
                                                                                                                                  cTCAGTACTCTTCATCTTCAATCAAACGCTGACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCtttt                 <  2:1186567/150‑1 (MQ=255)
                                                                                                                                    cAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTtc               <  1:1195639/150‑1 (MQ=255)
                                                                                                                                      gTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTtctc             >  1:4059916/1‑150 (MQ=255)
                                                                                                                                                tcttcaATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCAGCCGATTa   <  1:4049555/150‑1 (MQ=255)
                                                                                                                                                 cttcaATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCAGCCGATTaa  >  2:482338/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCAGCCGATTAA  >  CP009273/2544941‑2545235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑