Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,633,402 C→A 5.3% R178L (CGC→CTC)  hisS ← histidyl tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,633,4020CA 5.3% 1040.3 / 21.3 269R178L (CGC→CTC) hisShistidyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (132/122);  new base A (6/9);  total (138/131)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

CACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGC  >  CP009273/2633256‑2633549
                                                                                                                                                  |                                                                                                                                                   
 aCCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTa                                                                                                                                                 <  1:3616024/150‑1 (MQ=255)
   cTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATTCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAAt                                                                                                                                               >  2:1808581/1‑150 (MQ=255)
    tAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAAtt                                                                                                                                              <  1:1480926/150‑1 (MQ=255)
     aaTGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTACAGCTTTTCTTTATGCTGCTCAAGGAAAGCCACCAGCGCATCGAGGTAATTg                                                                                                                                             >  1:2418436/1‑150 (MQ=255)
      aTGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTgg                                                                                                                                            <  2:1798375/150‑1 (MQ=255)
         ccGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGgcgc                                                                                                                                         >  2:3549521/1‑150 (MQ=255)
          cGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCCGCGCATCGCGGTAATTGGcgcg                                                                                                                                        >  2:916319/1‑150 (MQ=255)
          cGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGcgcg                                                                                                                                        <  2:873049/150‑1 (MQ=255)
          cGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGATCAAGGAATGCCACCAGCGCATCGCGGTAATTGGcgcg                                                                                                                                        >  2:2084013/1‑150 (MQ=255)
             aGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGc                                                                                                                                     <  1:74747/150‑1 (MQ=255)
             aGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGc                                                                                                                                     <  1:3096790/150‑1 (MQ=255)
                 tcgtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTcc                                                                                                                                 <  2:3952213/150‑1 (MQ=255)
                 tcgcTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCACAAGGAATGCCACCAGCGCATCGATGTAATTGGCGCGTGCTTcc                                                                                                                                 <  2:3100092/150‑1 (MQ=255)
                    ttGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGc                                                                                                                              <  1:1961547/150‑1 (MQ=255)
                    ttGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGc                                                                                                                              <  1:1961602/150‑1 (MQ=255)
                     tGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCg                                                                                                                             >  2:710629/1‑150 (MQ=255)
                         aaGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAAcc                                                                                                                         <  2:2783021/150‑1 (MQ=255)
                         aaGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAAcc                                                                                                                         >  1:1037680/1‑150 (MQ=255)
                          aGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCg                                                                                                                        >  1:110851/1‑150 (MQ=255)
                            cgcCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGAt                                                                                                                      <  1:4402299/150‑1 (MQ=255)
                            cgcCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGAt                                                                                                                      <  1:3660261/150‑1 (MQ=255)
                             gcCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATa                                                                                                                     <  2:3861486/150‑1 (MQ=255)
                                   aCTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTc                                                                                                               <  2:322411/150‑1 (MQ=255)
                                    cTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCa                                                                                                              >  1:2294032/1‑150 (MQ=255)
                                     ttCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAg                                                                                                             <  2:2994889/150‑1 (MQ=255)
                                     ttCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAg                                                                                                             <  2:196256/150‑1 (MQ=255)
                                     ttCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAg                                                                                                             <  2:197163/150‑1 (MQ=255)
                                     ttCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAg                                                                                                             <  2:2501374/150‑1 (MQ=255)
                                      tCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGc                                                                                                            >  2:3888111/1‑150 (MQ=255)
                                      tCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGc                                                                                                            <  1:1007242/150‑1 (MQ=255)
                                           aTTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCTAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCATCGAACCGATAGTGTTCAGGTCAag                                                                                                       >  2:2840490/1‑150 (MQ=255)
                                               tttGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGtt                                                                                                   <  2:2684147/150‑1 (MQ=255)
                                                ttGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTa                                                                                                  <  1:1620275/150‑1 (MQ=255)
                                                    aTCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTg                                                                                              >  2:4365682/1‑150 (MQ=255)
                                                     tCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGc                                                                                             >  2:429971/1‑150 (MQ=255)
                                                       cAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTc                                                                                           >  2:3699281/1‑150 (MQ=255)
                                                       cAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTc                                                                                           <  2:3262763/150‑1 (MQ=255)
                                                       cAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTc                                                                                           >  2:1194288/1‑150 (MQ=255)
                                                           aCGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGaa                                                                                       >  2:4698/1‑150 (MQ=255)
                                                           aCGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGaa                                                                                       >  1:456801/1‑150 (MQ=255)
                                                            cgcgCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGaaa                                                                                      <  2:3713486/150‑1 (MQ=255)
                                                             gcgcAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAAt                                                                                     <  1:2574534/150‑1 (MQ=255)
                                                              cgcAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATa                                                                                    <  2:1031373/150‑1 (MQ=255)
                                                               gcAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATAc                                                                                   <  2:2940674/150‑1 (MQ=255)
                                                                  gCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCa                                                                                <  1:636071/150‑1 (MQ=255)
                                                                    gggTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgc                                                                              <  2:1131634/150‑1 (MQ=255)
                                                                       ttAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgcgcg                                                                           >  2:4334665/1‑150 (MQ=255)
                                                                       ttAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgcgcg                                                                           <  2:2305417/150‑1 (MQ=255)
                                                                       ttAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgcgcg                                                                           <  1:1192906/150‑1 (MQ=255)
                                                                       ttAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgcgcg                                                                           >  2:4055385/1‑150 (MQ=255)
                                                                         aGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgcgcgcg                                                                         >  1:3355819/1‑150 (MQ=255)
                                                                            gtACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGcca                                                                      <  1:120527/150‑1 (MQ=255)
                                                                             tACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGccac                                                                     >  2:2160964/1‑150 (MQ=255)
                                                                             tACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGccac                                                                     >  2:3530140/1‑150 (MQ=255)
                                                                              aCATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGccacc                                                                    <  1:1764635/150‑1 (MQ=255)
                                                                               cATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGccacca                                                                   <  2:1608392/150‑1 (MQ=255)
                                                                                aTGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAg                                                                  <  1:2698419/150‑1 (MQ=255)
                                                                                 tGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGc                                                                 <  1:948833/150‑1 (MQ=255)
                                                                                    ggcgTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCggg                                                              <  1:3577776/150‑1 (MQ=255)
                                                                                    ggcgTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCggg                                                              >  1:628040/1‑150 (MQ=255)
                                                                                     gcgTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGc                                                             <  1:1267429/150‑1 (MQ=255)
                                                                                     gcgTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGAGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGc                                                             <  2:3585190/150‑1 (MQ=255)
                                                                                      cgTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCa                                                            >  1:3849625/1‑150 (MQ=255)
                                                                                      cgTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCa                                                            >  2:3061623/1‑150 (MQ=255)
                                                                                          tGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGa                                                        >  1:2555949/1‑150 (MQ=255)
                                                                                           gCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAg                                                       >  1:4053124/1‑150 (MQ=255)
                                                                                           gCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAg                                                       <  2:3402618/150‑1 (MQ=255)
                                                                                            cAGTCTTCGTCCAGCTTTTCTGTATGCTGCTCAAGGACTGCCACCAGCGCATCGCGGGAATTGGCGCGGGCTTCAAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGACATACCCAGCGCGCGCCACCAGCGGGCAGTGAGc                                                      >  2:3135871/1‑150 (MQ=255)
                                                                                             aGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCa                                                     <  2:1739053/150‑1 (MQ=255)
                                                                                                cTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATaa                                                  <  2:2942343/150‑1 (MQ=255)
                                                                                                cTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATaa                                                  <  2:4561681/150‑1 (MQ=255)
                                                                                                    gTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAg                                              >  1:4317896/1‑150 (MQ=255)
                                                                                                    gTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAg                                              >  2:441791/1‑150 (MQ=255)
                                                                                                        aGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCa                                          <  2:3689005/150‑1 (MQ=255)
                                                                                                         gCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAg                                         >  1:2000696/1‑150 (MQ=255)
                                                                                                         gCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAg                                         <  1:3315109/150‑1 (MQ=255)
                                                                                                          cTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGc                                        <  2:654015/150‑1 (MQ=255)
                                                                                                             ttCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTc                                     >  1:2421742/1‑150 (MQ=255)
                                                                                                               cTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGa                                   >  2:1800934/1‑150 (MQ=255)
                                                                                                                  tATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCCCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGatat                                <  2:4068253/150‑1 (MQ=255)
                                                                                                                   aTGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATc                               >  2:3253377/1‑150 (MQ=255)
                                                                                                                   aTGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATc                               >  1:413355/1‑150 (MQ=255)
                                                                                                                   aTGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACACAGCTCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATc                               >  1:1701015/1‑150 (MQ=255)
                                                                                                                    tgctgcTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATcc                              <  2:529157/150‑1 (MQ=255)
                                                                                                                      ctgctCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCgg                            >  2:3227326/1‑150 (MQ=255)
                                                                                                                      ctgctCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCgg                            >  1:2981839/1‑150 (MQ=255)
                                                                                                                       tgctCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGa                           <  1:1908735/150‑1 (MQ=255)
                                                                                                                                aaTGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGa                  >  1:4055328/1‑150 (MQ=255)
                                                                                                                                 aTGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGAc                 >  2:861363/1‑150 (MQ=255)
                                                                                                                                 aTGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGAc                 <  1:819220/150‑1 (MQ=255)
                                                                                                                                  tGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGAcc                >  2:2888085/1‑150 (MQ=255)
                                                                                                                                     caccaGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaa             <  1:1449667/150‑1 (MQ=255)
                                                                                                                                       ccaGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaaaa           >  2:926293/1‑150 (MQ=255)
                                                                                                                                       ccaGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaaaa           <  2:4174152/150‑1 (MQ=255)
                                                                                                                                        caGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAAc          >  2:237595/1‑150 (MQ=255)
                                                                                                                                           cgcATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTc       <  1:1591878/150‑1 (MQ=255)
                                                                                                                                             cATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGc     <  2:4227934/150‑1 (MQ=255)
                                                                                                                                             cATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGc     <  1:2109620/150‑1 (MQ=255)
                                                                                                                                              aTCGCGGGAATTCGCGCGTGCTTCCAGCGAACCGAAAGACTTAAGCTCAAAAGATACGAGCACGCAAATAACAAACTCGCGCCACCAGCGCGCAGAGAGCATAATCACTTCAGCGTCGATATCCGGAACATGAAGACCGAAAACTACGCa    >  2:4272703/1‑150 (MQ=255)
                                                                                                                                                cgcgGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGc  >  2:1103699/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGCAGC  >  CP009273/2633256‑2633549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑