Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,664,675 C→A 5.2% A31E (GCG→GAG)  hcaB → 2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,664,6750CA 5.2% 1057.8 / 22.4 273A31E (GCG→GAG) hcaB2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (121/137);  new base A (5/10);  total (126/147)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 281 total aligned reads displayed.

CTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAGATCCTGACTCGTTCCGGCAAGC  >  CP009273/2664526‑2664824
                                                                                                                                                     |                                                                                                                                                     
cTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGgcgc                                                                                                                                                       <  2:441600/150‑1 (MQ=255)
 ttATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGgcgcg                                                                                                                                                      >  1:1110753/1‑150 (MQ=255)
  tATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGgcgcgc                                                                                                                                                     <  2:4136181/150‑1 (MQ=255)
   aTCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCa                                                                                                                                                    >  2:1070400/1‑150 (MQ=255)
       aGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGtt                                                                                                                                                <  2:2633245/150‑1 (MQ=255)
        gTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTg                                                                                                                                               <  1:2131444/150‑1 (MQ=255)
          acacGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTATGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGcc                                                                                                                                             >  1:2416963/1‑150 (MQ=255)
          acacGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGcc                                                                                                                                             >  2:2816877/1‑150 (MQ=255)
            acGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGAGCAGGTTGCCAc                                                                                                                                           <  2:4212304/150‑1 (MQ=255)
               ttGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGTAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCt                                                                                                                                        <  2:1722676/150‑1 (MQ=255)
               ttGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCt                                                                                                                                        >  1:2826772/1‑150 (MQ=255)
                  aaGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGa                                                                                                                                     >  1:3822586/1‑150 (MQ=255)
                  aaGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGa                                                                                                                                     >  1:1921852/1‑150 (MQ=255)
                  aaGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGAGCAGGTTGCCACGCTGGa                                                                                                                                     >  1:3972579/1‑150 (MQ=255)
                   aGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGaa                                                                                                                                    >  1:1425683/1‑150 (MQ=255)
                     gtggtgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACt                                                                                                                                  <  2:1330661/150‑1 (MQ=255)
                       ggtgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGACCTgc                                                                                                                                >  1:2720627/1‑149 (MQ=255)
                       ggtgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGt                                                                                                                                >  1:398662/1‑150 (MQ=255)
                       ggtgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATAGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGt                                                                                                                                >  1:1159162/1‑150 (MQ=255)
                        gtgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTc                                                                                                                               <  1:1311328/150‑1 (MQ=255)
                        gtgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTc                                                                                                                               <  1:1311295/150‑1 (MQ=255)
                        gtgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTc                                                                                                                               >  2:2460584/1‑150 (MQ=255)
                        gngACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTc                                                                                                                               >  2:149123/1‑150 (MQ=255)
                         tgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTcg                                                                                                                              <  1:538197/150‑1 (MQ=255)
                           aCATTTTCATCGACTTACCAGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTcggc                                                                                                                            >  1:2840835/1‑150 (MQ=255)
                             aTTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTcggcgg                                                                                                                          >  1:2939487/1‑150 (MQ=255)
                             aTTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTcggcgg                                                                                                                          >  2:2837715/1‑150 (MQ=255)
                               tttCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCa                                                                                                                        <  2:4148778/150‑1 (MQ=255)
                                ttCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCaa                                                                                                                       <  2:3165561/150‑1 (MQ=255)
                                  cATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCaaaa                                                                                                                     >  1:2192826/1‑150 (MQ=255)
                                  cATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCaaaa                                                                                                                     <  2:4439568/150‑1 (MQ=255)
                                   aTCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAg                                                                                                                    <  2:2169698/150‑1 (MQ=255)
                                   aTCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAg                                                                                                                    >  1:1740702/1‑150 (MQ=255)
                                    tCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGt                                                                                                                   >  2:373992/1‑150 (MQ=255)
                                     cGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTc                                                                                                                  <  2:3962497/150‑1 (MQ=255)
                                     cGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGAGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTc                                                                                                                  <  2:636139/150‑1 (MQ=255)
                                        cTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGcc                                                                                                               <  1:229331/150‑1 (MQ=255)
                                          tACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAg                                                                                                             >  2:1207916/1‑150 (MQ=255)
                                              ggAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTg                                                                                                         >  1:1485323/1‑150 (MQ=255)
                                              ggAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTg                                                                                                         >  1:676301/1‑150 (MQ=255)
                                                aGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCg                                                                                                       <  1:4214379/150‑1 (MQ=255)
                                                aGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCg                                                                                                       <  1:997066/150‑1 (MQ=255)
                                                aGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGAGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCg                                                                                                       >  2:538734/1‑150 (MQ=255)
                                                          aTGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGAttt                                                                                             >  1:2514342/1‑150 (MQ=255)
                                                                 aTCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAAc                                                                                      <  2:1501623/150‑1 (MQ=255)
                                                                    tGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACata                                                                                   >  2:340355/1‑150 (MQ=255)
                                                                    tGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACata                                                                                   <  2:1910021/150‑1 (MQ=255)
                                                                         aaCGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTg                                                                              <  2:3324140/150‑1 (MQ=255)
                                                                           cGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGc                                                                            <  2:3513731/150‑1 (MQ=255)
                                                                           cGAGTCCATTTTTATTACCGGCGGCGGATCGGGAGTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGc                                                                            >  2:737656/1‑150 (MQ=255)
                                                                             aGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCgg                                                                          <  2:1671792/150‑1 (MQ=255)
                                                                                ccATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTgg                                                                       <  1:65020/150‑1 (MQ=255)
                                                                                ccATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTgg                                                                       <  2:2137496/150‑1 (MQ=255)
                                                                                 cATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGa                                                                      >  1:3653551/1‑150 (MQ=255)
                                                                                  aTTTTTATTCCCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGaa                                                                     <  2:2336375/150‑1 (MQ=255)
                                                                                  aTTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGaa                                                                     <  1:1511104/150‑1 (MQ=255)
                                                                                  aTTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGAGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGaa                                                                     <  1:2081022/150‑1 (MQ=255)
                                                                                   tttttATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAg                                                                    <  1:627594/150‑1 (MQ=255)
                                                                                   tttttATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAg                                                                    >  1:1370330/1‑150 (MQ=255)
                                                                                   tttttATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAg                                                                    >  1:2662381/1‑150 (MQ=255)
                                                                                       tattaCCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTaa                                                                <  1:3835760/150‑1 (MQ=255)
                                                                                       tattaCCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTaa                                                                >  1:395807/1‑150 (MQ=255)
                                                                                        attaCCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAAc                                                               >  1:286631/1‑150 (MQ=255)
                                                                                         ttaCCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACg                                                              <  1:2337704/150‑1 (MQ=255)
                                                                                          taCCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGt                                                             >  1:3080093/1‑150 (MQ=255)
                                                                                          taCCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGt                                                             >  2:3833713/1‑150 (MQ=255)
                                                                                           aCCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTg                                                            >  1:4622384/1‑150 (MQ=255)
                                                                                            ccGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGa                                                           >  2:4131251/1‑150 (MQ=255)
                                                                                            ccGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGa                                                           <  1:3270128/150‑1 (MQ=255)
                                                                                              ggcggcggATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGAcc                                                         <  1:3027858/150‑1 (MQ=255)
                                                                                                 ggcggATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGt                                                      <  1:3438766/150‑1 (MQ=255)
                                                                                                  gcggATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGtt                                                     <  2:346482/150‑1 (MQ=255)
                                                                                                   cggATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTa                                                    <  2:2525503/150‑1 (MQ=255)
                                                                                                   cggATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTa                                                    >  2:1595459/1‑150 (MQ=255)
                                                                                                    ggATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTAt                                                   <  2:3159616/150‑1 (MQ=255)
                                                                                                     gATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATg                                                  <  1:772392/150‑1 (MQ=255)
                                                                                                       tCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGcc                                                <  1:3155395/150‑1 (MQ=255)
                                                                                                         gggATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGa                                              >  1:4225082/1‑150 (MQ=255)
                                                                                                             ttAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTAt                                          <  2:3987596/150‑1 (MQ=255)
                                                                                                              tAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATc                                         >  1:4475860/1‑150 (MQ=255)
                                                                                                               aGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCa                                        >  1:4569337/1‑150 (MQ=255)
                                                                                                                gggCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCaa                                       >  2:2900731/1‑150 (MQ=255)
                                                                                                                gggCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCaa                                       >  2:2761912/1‑150 (MQ=255)
                                                                                                                   cTGACGCTGGTCGAGCGATTTATCGAAGAAGGCGACCAGTTTGCCACGCTGGAACTGACGGCGGCAAAAGTCGCCAGACTCCGTCAGCGATTTGGCGACCAAAATCTGGCGGTGGAAGGAAACGAGACCTCTTATGCAGATTATCAAcgc                                    >  2:1767993/1‑150 (MQ=255)
                                                                                                                         cTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTAGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTc                              <  2:4628745/150‑1 (MQ=255)
                                                                                                                          tGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCg                             >  1:336622/1‑150 (MQ=255)
                                                                                                                          tGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCg                             >  2:4164012/1‑150 (MQ=255)
                                                                                                                               gAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAg                        <  2:4082938/150‑1 (MQ=255)
                                                                                                                               gAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAg                        <  1:1567213/150‑1 (MQ=255)
                                                                                                                                aGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAGa                       <  1:59651/150‑1 (MQ=255)
                                                                                                                                 gCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAGAt                      <  1:4314924/150‑1 (MQ=255)
                                                                                                                                 gCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAGAt                      <  1:4505844/150‑1 (MQ=255)
                                                                                                                                  cGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAGATc                     <  2:3438077/150‑1 (MQ=255)
                                                                                                                                   gATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAGATcc                    >  2:1815364/1‑150 (MQ=255)
                                                                                                                                    aTTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAGATCCt                   <  2:2220540/150‑1 (MQ=255)
                                                                                                                                    aTTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAGATCCt                   <  2:1119557/150‑1 (MQ=255)
                                                                                                                                     tttATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAGATCCTg                  <  2:1564529/150‑1 (MQ=255)
                                                                                                                                        aTCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAGATCCTGACt               >  1:3740384/1‑150 (MQ=255)
                                                                                                                                          cGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAGATCCTGACTCg             >  1:1283970/1‑150 (MQ=255)
                                                                                                                                               aagGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAGATCCTGACTCGTTCCg        <  2:3206212/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAGATCCTGACTCGTTCCGGCAAGC  >  CP009273/2664526‑2664824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑