Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,840,562 C→A 17.0% V70V (GTG→GTT)  hycD ← hydrogenase 3, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,840,5620CA17.0% 879.2 / 105.2 278V70V (GTG→GTT) hycDhydrogenase 3, membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (129/101);  new base A (38/10);  total (167/111)
Fisher's exact test for biased strand distribution p-value = 3.29e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

CTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGTTATACCGGA  >  CP009273/2840413‑2840711
                                                                                                                                                     |                                                                                                                                                     
  aCCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATcac                                                                                                                                                     >  2:2418917/1‑150 (MQ=255)
   ccGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATcaca                                                                                                                                                    <  2:3490035/150‑1 (MQ=255)
     ggTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATa                                                                                                                                                  >  2:1064018/1‑150 (MQ=255)
     ggTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATa                                                                                                                                                  >  2:2568148/1‑150 (MQ=255)
      gTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATAc                                                                                                                                                 >  2:1877684/1‑150 (MQ=255)
       tATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACg                                                                                                                                                >  1:2856869/1‑150 (MQ=255)
              accagaAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAg                                                                                                                                         <  1:4027495/150‑1 (MQ=255)
               ccagaAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAgg                                                                                                                                        <  2:2433584/150‑1 (MQ=255)
                  gaAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGAGAGTCAGCATGACGCCCCCCATCACATACGGCGTCAGGCgg                                                                                                                                     >  2:3675948/1‑150 (MQ=255)
                    aaTGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGaa                                                                                                                                   >  2:551313/1‑150 (MQ=255)
                    aaTGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGaa                                                                                                                                   >  1:3556650/1‑150 (MQ=255)
                        gCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACAcc                                                                                                                               >  2:57596/1‑150 (MQ=255)
                        gCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACAcc                                                                                                                               <  2:771216/150‑1 (MQ=255)
                         cAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACAccc                                                                                                                              <  1:211823/150‑1 (MQ=255)
                          aaaGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCa                                                                                                                             <  2:3375156/150‑1 (MQ=255)
                          aaaGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCa                                                                                                                             <  1:2220806/150‑1 (MQ=255)
                           aagaagAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAg                                                                                                                            <  2:504697/150‑1 (MQ=255)
                            agaagaAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGc                                                                                                                           >  2:1565019/1‑150 (MQ=255)
                            agaagaAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGc                                                                                                                           >  2:430814/1‑150 (MQ=255)
                              aagaaACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCg                                                                                                                         <  2:2291816/150‑1 (MQ=255)
                              aagaaACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCg                                                                                                                         >  2:3956541/1‑150 (MQ=255)
                                gaaACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGa                                                                                                                       >  1:3393795/1‑150 (MQ=255)
                                  aaCGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAgg                                                                                                                     >  1:2521876/1‑150 (MQ=255)
                                  aaCGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAgg                                                                                                                     >  1:1835660/1‑150 (MQ=255)
                                  aaCGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAgg                                                                                                                     >  2:820137/1‑150 (MQ=255)
                                   aCGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGc                                                                                                                    >  2:4470899/1‑150 (MQ=255)
                                      cgcgATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATc                                                                                                                 >  1:3725860/1‑150 (MQ=255)
                                        cgATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCg                                                                                                               <  1:3094119/150‑1 (MQ=255)
                                         gATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGTCGTCAGGCGGAACACCCAGCCGGAGGCATCCgg                                                                                                              >  1:1107738/1‑150 (MQ=255)
                                         gATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCgg                                                                                                              <  1:3755690/150‑1 (MQ=255)
                                            ggCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGcc                                                                                                           <  2:2098679/150‑1 (MQ=255)
                                            ggCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGcc                                                                                                           <  2:1688867/150‑1 (MQ=255)
                                            ggCAAAGAGAGACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGcc                                                                                                           <  1:4414435/150‑1 (MQ=255)
                                              cAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGa                                                                                                         <  1:1030179/150‑1 (MQ=255)
                                              cAAAGAGATACAGTAAGGTGATCAAAACACCCAGTAGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATCGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGa                                                                                                         >  1:1641671/1‑150 (MQ=255)
                                                aaGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACg                                                                                                       >  2:2968308/1‑150 (MQ=255)
                                                   agaTACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGATGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGctc                                                                                                    >  1:2996943/1‑150 (MQ=255)
                                                   agaTACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGctc                                                                                                    >  1:1613308/1‑150 (MQ=255)
                                                   agaTACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGctc                                                                                                    <  2:1250497/150‑1 (MQ=255)
                                                    gaTACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGctct                                                                                                   >  2:965151/1‑150 (MQ=255)
                                                      tACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGa                                                                                                 <  2:324964/150‑1 (MQ=255)
                                                        cAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACg                                                                                               >  2:1552683/1‑150 (MQ=255)
                                                             aGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCca                                                                                          <  1:4161910/150‑1 (MQ=255)
                                                             aGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCca                                                                                          <  1:4161868/150‑1 (MQ=255)
                                                               gTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCcagc                                                                                        >  1:3281984/1‑150 (MQ=255)
                                                                      aaTCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGa                                                                                 >  1:1267118/1‑150 (MQ=255)
                                                                        tCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATa                                                                               >  2:2724521/1‑150 (MQ=255)
                                                                         cACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATaa                                                                              <  2:4044758/150‑1 (MQ=255)
                                                                         cACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATaa                                                                              <  1:4518444/150‑1 (MQ=255)
                                                                         cACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATaa                                                                              >  2:1388916/1‑150 (MQ=255)
                                                                           cccAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATg                                                                            <  2:3558632/150‑1 (MQ=255)
                                                                             cAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTc                                                                          >  2:70682/1‑150 (MQ=255)
                                                                              aGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTcg                                                                         >  1:994187/1‑150 (MQ=255)
                                                                              aGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTcg                                                                         >  1:2044662/1‑150 (MQ=255)
                                                                                ttGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTcgcg                                                                       <  2:1802878/150‑1 (MQ=255)
                                                                                 tGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGa                                                                      <  2:1670492/150‑1 (MQ=255)
                                                                                 tGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGa                                                                      >  1:2637445/1‑150 (MQ=255)
                                                                                    ggCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATAc                                                                   >  2:1166892/1‑150 (MQ=255)
                                                                                    ggCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATAc                                                                   >  2:1162115/1‑150 (MQ=255)
                                                                                      cAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTc                                                                 >  1:4241043/1‑150 (MQ=255)
                                                                                       aGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTcc                                                                <  1:2294440/150‑1 (MQ=255)
                                                                                          ggAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGc                                                             >  2:2855447/1‑150 (MQ=255)
                                                                                          ggAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGc                                                             >  1:1527757/1‑150 (MQ=255)
                                                                                          ggAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGc                                                             >  2:288286/1‑150 (MQ=255)
                                                                                           gagaACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCa                                                            >  2:4550920/1‑150 (MQ=255)
                                                                                           gagaACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCa                                                            <  2:2014432/150‑1 (MQ=255)
                                                                                              aaCCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAaca                                                         <  2:3822487/150‑1 (MQ=255)
                                                                                              aaCCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAaca                                                         >  2:2214869/1‑150 (MQ=255)
                                                                                               aCCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAacac                                                        <  1:1923395/150‑1 (MQ=255)
                                                                                               aCCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAacac                                                        <  2:2738803/150‑1 (MQ=255)
                                                                                                  gACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACgcc                                                     >  1:3698049/1‑150 (MQ=255)
                                                                                                  gACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACgcc                                                     >  1:1801017/1‑150 (MQ=255)
                                                                                                       tCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACgcccggcc                                                <  2:4531741/150‑1 (MQ=255)
                                                                                                        caccacCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACgcccggccc                                               <  2:309237/150‑1 (MQ=255)
                                                                                                           caccGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCcgcg                                            >  1:4583351/1‑150 (MQ=255)
                                                                                                                 cAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGtt                                      >  1:911492/1‑150 (MQ=255)
                                                                                                                  aGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTa                                     <  2:1343797/150‑1 (MQ=255)
                                                                                                                     gcAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGc                                  <  2:2640060/150‑1 (MQ=255)
                                                                                                                      cAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCa                                 >  2:179504/1‑150 (MQ=255)
                                                                                                                      cAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCa                                 >  2:1740266/1‑150 (MQ=255)
                                                                                                                          aGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCg                             >  1:1493708/1‑150 (MQ=255)
                                                                                                                           gCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCgg                            <  1:1066766/150‑1 (MQ=255)
                                                                                                                            cGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCggg                           >  2:3700451/1‑150 (MQ=255)
                                                                                                                             gATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGgc                          >  1:1172740/1‑150 (MQ=255)
                                                                                                                             gATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGgc                          >  1:2758832/1‑150 (MQ=255)
                                                                                                                              aTAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGgcg                         >  1:745554/1‑150 (MQ=255)
                                                                                                                               tAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGgcgc                        >  1:1320056/1‑150 (MQ=255)
                                                                                                                               tAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGgcgc                        <  1:3531733/150‑1 (MQ=255)
                                                                                                                                aGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGgcgcg                       <  1:2330185/150‑1 (MQ=255)
                                                                                                                                      cATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCAc                 >  2:437344/1‑150 (MQ=255)
                                                                                                                                        tGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCAcgc               >  1:1376595/1‑150 (MQ=255)
                                                                                                                                         gACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCAcgcg              >  1:642258/1‑150 (MQ=255)
                                                                                                                                          aCGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCgg             <  2:3398871/150‑1 (MQ=255)
                                                                                                                                           cGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCggg            <  2:3469718/150‑1 (MQ=255)
                                                                                                                                            gCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGt           >  1:3116689/1‑150 (MQ=255)
                                                                                                                                             cccACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGtt          >  1:4369263/1‑150 (MQ=255)
                                                                                                                                             cccACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGtt          >  1:2277497/1‑150 (MQ=255)
                                                                                                                                             cccACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGtt          >  2:2209507/1‑150 (MQ=255)
                                                                                                                                              ccaccaTCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCCGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGTta         <  2:623611/150‑1 (MQ=255)
                                                                                                                                                 ccaTCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGTTATAc      >  2:2458894/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGTTATACCGGA  >  CP009273/2840413‑2840711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑