Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,841,044 C→A 100% A519A (GCG→GCT)  hycC ← hydrogenase 3, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,841,0440CA99.4% 1153.0 / ‑1.7 326A519A (GCG→GCT) hycChydrogenase 3, membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (152/172);  total (154/172)
Fisher's exact test for biased strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 326 total aligned reads displayed.

TTGCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAAT  >  CP009273/2840898‑2841190
                                                                                                                                                  |                                                                                                                                                  
ttGCGTAGTTTCTTCACCGTAGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCTGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGcc                                                                                                                                                 <  1:1454647/150‑1 (MQ=255)
     tAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAat                                                                                                                                            <  1:3157421/150‑1 (MQ=255)
       gTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAatga                                                                                                                                          >  1:1041742/1‑150 (MQ=255)
         ttCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAatgatg                                                                                                                                        <  1:939712/150‑1 (MQ=255)
          tCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAAtgatga                                                                                                                                       <  2:2436527/150‑1 (MQ=255)
          tCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAAtgatga                                                                                                                                       <  2:2895082/150‑1 (MQ=255)
            aGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAAt                                                                                                                                     <  1:394068/150‑1 (MQ=255)
            aGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAAt                                                                                                                                     <  1:2859929/150‑1 (MQ=255)
             gCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATg                                                                                                                                    <  2:2605860/150‑1 (MQ=255)
             gCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATg                                                                                                                                    <  1:4356000/150‑1 (MQ=255)
               aCCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgc                                                                                                                                  <  2:326337/150‑1 (MQ=255)
                 cGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgcag                                                                                                                                >  1:3881822/1‑150 (MQ=255)
                 cGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgcag                                                                                                                                >  1:233748/1‑150 (MQ=255)
                 cGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgcag                                                                                                                                <  1:2386870/150‑1 (MQ=255)
                  ggCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgcagc                                                                                                                               >  2:4612097/1‑150 (MQ=255)
                  ggCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgcagc                                                                                                                               >  1:3049176/1‑150 (MQ=255)
                   gcgcAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgcagca                                                                                                                              <  2:3541225/150‑1 (MQ=255)
                    cgcAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGcagcag                                                                                                                             >  1:2937730/1‑150 (MQ=255)
                     gcAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCagcagc                                                                                                                            <  2:2822849/150‑1 (MQ=255)
                       aaaCGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCgg                                                                                                                          <  1:288722/150‑1 (MQ=255)
                       aaaCGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCgg                                                                                                                          >  1:2451652/1‑150 (MQ=255)
                       aaaCGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCgg                                                                                                                          <  2:4281172/150‑1 (MQ=255)
                           gCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcag                                                                                                                      <  1:3247/150‑1 (MQ=255)
                            ccTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcagg                                                                                                                     >  1:4545394/1‑150 (MQ=255)
                              tGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcaggca                                                                                                                   <  2:2518255/150‑1 (MQ=255)
                              tGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcaggca                                                                                                                   <  2:1239244/150‑1 (MQ=255)
                              tGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcaggca                                                                                                                   <  1:1162115/150‑1 (MQ=255)
                               gTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCaa                                                                                                                  <  2:4042838/150‑1 (MQ=255)
                                tttCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAAt                                                                                                                 <  1:594090/150‑1 (MQ=255)
                                 ttaaCCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATc                                                                                                                <  2:3355851/147‑1 (MQ=255)
                                 ttCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATc                                                                                                                >  2:730502/1‑150 (MQ=255)
                                  tCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATca                                                                                                               <  2:1341738/150‑1 (MQ=255)
                                  tCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATca                                                                                                               <  1:237049/150‑1 (MQ=255)
                                   cACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCACTcag                                                                                                              >  1:837969/1‑150 (MQ=255)
                                   cACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcag                                                                                                              <  2:878679/150‑1 (MQ=255)
                                     ccGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcagca                                                                                                            <  2:1390802/150‑1 (MQ=255)
                                      cGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcagcag                                                                                                           <  2:1614939/150‑1 (MQ=255)
                                         cATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCaa                                                                                                        <  1:1416778/150‑1 (MQ=255)
                                          aTGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAAc                                                                                                       >  2:3738991/1‑150 (MQ=255)
                                          aTGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAAc                                                                                                       >  1:4392353/1‑150 (MQ=255)
                                          aTGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAAc                                                                                                       >  1:2077392/1‑150 (MQ=255)
                                               aaaaCCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGat                                                                                                  <  1:2756773/150‑1 (MQ=255)
                                                aaaCCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGatc                                                                                                 <  2:1799129/150‑1 (MQ=255)
                                                 aaCCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGatca                                                                                                >  2:3401776/1‑150 (MQ=255)
                                                   ccGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGatcatc                                                                                              <  1:3928839/150‑1 (MQ=255)
                                                    cGTGAGCGGTAATCACCATTGATTTTTTGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCTGCAACGTGATCATCg                                                                                             <  2:2312256/150‑1 (MQ=255)
                                                      tGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGt                                                                                           <  1:2071439/150‑1 (MQ=255)
                                                      tGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGt                                                                                           >  2:186726/1‑150 (MQ=255)
                                                        aGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTg                                                                                         <  1:678399/150‑1 (MQ=255)
                                                           ggTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTgaga                                                                                      <  2:4085145/150‑1 (MQ=255)
                                                                tCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACgg                                                                                 >  2:1054593/1‑150 (MQ=255)
                                                                 cACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACggt                                                                                <  1:3518088/150‑1 (MQ=255)
                                                                 cACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACggt                                                                                <  1:3642711/150‑1 (MQ=255)
                                                                  aCCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACggtg                                                                               <  1:2581269/150‑1 (MQ=255)
                                                                   ccATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAACCggtgg                                                                              >  2:2313600/1‑150 (MQ=255)
                                                                    cATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACggtggt                                                                             <  1:1587586/150‑1 (MQ=255)
                                                                       tGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGtt                                                                          <  1:2649252/150‑1 (MQ=255)
                                                                         aTTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAg                                                                        >  1:925468/1‑150 (MQ=255)
                                                                          tttttCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGc                                                                       <  1:3796015/150‑1 (MQ=255)
                                                                          tttttCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGc                                                                       >  1:3486618/1‑150 (MQ=255)
                                                                          tttttCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGc                                                                       <  1:552103/150‑1 (MQ=255)
                                                                            tttCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAg                                                                     <  1:3598178/150‑1 (MQ=255)
                                                                                gTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTc                                                                 <  1:3725942/150‑1 (MQ=255)
                                                                                gTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTc                                                                 >  1:2787679/1‑150 (MQ=255)
                                                                                  ggTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCa                                                               >  2:1145885/1‑150 (MQ=255)
                                                                                   gTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAg                                                              <  1:3106058/150‑1 (MQ=255)
                                                                                   gTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAg                                                              <  1:3669600/150‑1 (MQ=255)
                                                                                       tAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGc                                                          >  2:3796007/1‑150 (MQ=255)
                                                                                       tAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGc                                                          >  2:4331297/1‑150 (MQ=255)
                                                                                         aCCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCag                                                        >  2:833438/1‑150 (MQ=255)
                                                                                         aCCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCag                                                        <  1:527647/150‑1 (MQ=255)
                                                                                              acacCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTa                                                   >  2:1725183/1‑150 (MQ=255)
                                                                                              acacCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTa                                                   <  2:1564337/150‑1 (MQ=255)
                                                                                              acacCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTa                                                   <  2:209537/150‑1 (MQ=255)
                                                                                                acCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAca                                                 <  1:4237966/150‑1 (MQ=255)
                                                                                                 cccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcag                                                >  2:4490198/1‑150 (MQ=255)
                                                                                                 cccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcag                                                <  1:1726917/150‑1 (MQ=255)
                                                                                                   cAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagca                                              >  2:4446662/1‑150 (MQ=255)
                                                                                                   cAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagca                                              >  2:3495348/1‑150 (MQ=255)
                                                                                                    aGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcag                                             >  2:4392255/1‑150 (MQ=255)
                                                                                                       ccGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcagcag                                          >  1:4556165/1‑150 (MQ=255)
                                                                                                        cGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCaga                                         >  1:1543829/1‑150 (MQ=255)
                                                                                                          cACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCagaga                                       <  2:4218325/150‑1 (MQ=255)
                                                                                                             cgcgGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCa                                    <  2:3468205/150‑1 (MQ=255)
                                                                                                              gcgGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCAt                                   >  1:2161142/1‑150 (MQ=255)
                                                                                                               cgGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCACTCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATc                                  >  2:2924054/1‑150 (MQ=255)
                                                                                                               cgGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATc                                  >  1:1299880/1‑150 (MQ=255)
                                                                                                                 ggAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCgg                                >  2:2850229/1‑150 (MQ=255)
                                                                                                                  gAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGc                               >  2:498410/1‑150 (MQ=255)
                                                                                                                    aCGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAg                             <  2:4071089/150‑1 (MQ=255)
                                                                                                                      gcgATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTa                           <  1:4587238/150‑1 (MQ=255)
                                                                                                                       cgATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAg                          <  2:192830/150‑1 (MQ=255)
                                                                                                                       cgATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAg                          >  1:382307/1‑150 (MQ=255)
                                                                                                                         aTGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGcc                        <  2:3855766/150‑1 (MQ=255)
                                                                                                                                      gCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAac           >  2:3618880/1‑150 (MQ=255)
                                                                                                                                        cTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAacac         >  1:4054442/1‑150 (MQ=255)
                                                                                                                                         tttGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCCGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACAcc        >  2:3127762/1‑150 (MQ=255)
                                                                                                                                         tttGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACAcc        <  1:102807/150‑1 (MQ=255)
                                                                                                                                           tGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGc      >  2:3211169/1‑150 (MQ=255)
                                                                                                                                             cAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCa    <  1:4307478/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTGCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAAT  >  CP009273/2840898‑2841190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑