Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,893,446 T→A 94.4% K63* (AAA→TAA)  ygcW ← putative dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,893,4460TA94.4% 838.3 / 29.4 250K63* (AAA→TAA) ygcWputative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (5/9);  new base A (114/122);  total (119/131)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

Only 100 of 258 total aligned reads displayed.

GTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAA  >  CP009273/2893297‑2893594
                                                                                                                                                     |                                                                                                                                                    
   cAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTc                                                                                                                                                   >  2:580816/1‑150 (MQ=255)
        ccTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCa                                                                                                                                              >  2:3451510/1‑150 (MQ=255)
        ccTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCa                                                                                                                                              >  1:4114233/1‑150 (MQ=255)
         cTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCAt                                                                                                                                             <  2:4279449/150‑1 (MQ=255)
           tATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCAttt                                                                                                                                           >  2:3115800/1‑150 (MQ=255)
                aGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCttt                                                                                                                                      <  1:609936/150‑1 (MQ=255)
                  cTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGt                                                                                                                                    <  2:4506656/150‑1 (MQ=255)
                   ttACAAATACCGGCATTGTTAACCAGTATATCAACTGGACCGAAAAGCTCACTGCAAGCAGCGATACTCTTCTGCGGCGCGCCGTCTGCGGTGATTCCCACCTGCATGAAGTCCACCTCAACTCACTGTTATTCAATCATTTCCTTTggt                                                                                                                                   <  2:1471842/150‑3 (MQ=255)
                   ttACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGtt                                                                                                                                   >  2:3675170/1‑150 (MQ=255)
                    tACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGttt                                                                                                                                  <  2:3280394/150‑1 (MQ=255)
                    tACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGttt                                                                                                                                  >  1:3740340/1‑150 (MQ=255)
                      cAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCg                                                                                                                                <  1:2293864/150‑1 (MQ=255)
                      cAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCg                                                                                                                                >  1:4432647/1‑150 (MQ=255)
                      cAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCg                                                                                                                                <  2:104611/150‑1 (MQ=255)
                      cAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCg                                                                                                                                >  1:1451104/1‑150 (MQ=255)
                       aaaTACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGc                                                                                                                               >  1:2098224/1‑150 (MQ=255)
                        aaTACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGcc                                                                                                                              <  1:419596/150‑1 (MQ=255)
                         aTACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCg                                                                                                                             <  2:3924593/150‑1 (MQ=255)
                          tACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGt                                                                                                                            >  1:3068510/1‑150 (MQ=255)
                           aCCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGtt                                                                                                                           <  2:1919794/150‑1 (MQ=255)
                           aCCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGtt                                                                                                                           >  2:738432/1‑150 (MQ=255)
                           aCCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGtt                                                                                                                           <  2:613706/150‑1 (MQ=255)
                            ccGGCATTGTTAACCTGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTa                                                                                                                          <  1:86130/150‑1 (MQ=255)
                             cGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTAt                                                                                                                         <  1:4270721/150‑1 (MQ=255)
                              ggCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATc                                                                                                                        >  1:469304/1‑150 (MQ=255)
                              ggCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATc                                                                                                                        >  2:2831124/1‑150 (MQ=255)
                               gCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCt                                                                                                                       >  2:4614113/1‑150 (MQ=255)
                               gCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCt                                                                                                                       >  2:1621743/1‑150 (MQ=255)
                               gCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCt                                                                                                                       <  1:1788722/150‑1 (MQ=255)
                                 aTTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCttt                                                                                                                     <  2:3029663/150‑1 (MQ=255)
                                  ttGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTg                                                                                                                    <  1:261340/150‑1 (MQ=255)
                                  ttGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTg                                                                                                                    >  1:2764716/1‑150 (MQ=255)
                                   tGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGa                                                                                                                   >  1:530283/1‑150 (MQ=255)
                                    gTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGAc                                                                                                                  <  2:2812458/150‑1 (MQ=255)
                                     ttAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACg                                                                                                                 <  2:203421/150‑1 (MQ=255)
                                     ttAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACg                                                                                                                 >  2:2003/1‑150 (MQ=255)
                                      tAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGa                                                                                                                <  2:3428433/150‑1 (MQ=255)
                                         ccAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAAc                                                                                                             <  2:726592/150‑1 (MQ=255)
                                          cAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACt                                                                                                            >  1:3167044/1‑150 (MQ=255)
                                             aaTATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAgg                                                                                                         >  1:2371033/1‑150 (MQ=255)
                                               tatCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGaa                                                                                                       >  1:3243209/1‑150 (MQ=255)
                                                   aaCTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATaaa                                                                                                   >  2:2508606/1‑150 (MQ=255)
                                                     cTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGa                                                                                                 <  2:2333571/150‑1 (MQ=255)
                                                      tGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGat                                                                                                >  1:3331779/1‑150 (MQ=255)
                                                         aCCGAAACGCTCACAGCAAGCAGCGATAATCGTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATAtt                                                                                             <  2:1152419/150‑1 (MQ=255)
                                                           cGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTg                                                                                           <  1:645024/150‑1 (MQ=255)
                                                           cGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTg                                                                                           <  1:4469227/150‑1 (MQ=255)
                                                           cGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTg                                                                                           >  1:252607/1‑150 (MQ=255)
                                                           cGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTg                                                                                           >  1:2826669/1‑150 (MQ=255)
                                                            gAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTgc                                                                                          >  2:4547352/1‑150 (MQ=255)
                                                                cGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCa                                                                                      <  1:4550974/150‑1 (MQ=255)
                                                                  cTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGc                                                                                    <  1:1194464/150‑1 (MQ=255)
                                                                  cTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGc                                                                                    >  2:4610072/1‑150 (MQ=255)
                                                                  cTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGc                                                                                    >  1:1988861/1‑150 (MQ=255)
                                                                   tCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCt                                                                                   >  1:3927324/1‑150 (MQ=255)
                                                                   tCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCt                                                                                   >  2:2754245/1‑150 (MQ=255)
                                                                    cacaGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCtt                                                                                  >  2:1713922/1‑150 (MQ=255)
                                                                       agcaagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTgg                                                                               <  2:241790/150‑1 (MQ=255)
                                                                       agcaagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTgg                                                                               >  2:1354806/1‑150 (MQ=255)
                                                                          aagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCa                                                                            >  1:221662/1‑150 (MQ=255)
                                                                           agcagcGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCaa                                                                           >  1:4069049/1‑150 (MQ=255)
                                                                           agcagcGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCaa                                                                           >  1:2067146/1‑150 (MQ=255)
                                                                               gcGATACTCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGACGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAACCTAGGACTAAAGATATTTGCGCCAGCTTTGGCCAACGcc                                                                       >  2:4341983/1‑150 (MQ=255)
                                                                                cGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCa                                                                      <  2:909077/150‑1 (MQ=255)
                                                                                  aTAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATg                                                                    <  2:4222206/150‑1 (MQ=255)
                                                                                     aTCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCa                                                                 <  2:3626548/150‑1 (MQ=255)
                                                                                      tcttctGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCaa                                                                <  2:2694649/150‑1 (MQ=255)
                                                                                        ttctGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAAt                                                              <  2:133648/150‑1 (MQ=255)
                                                                                         tctGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATg                                                             <  2:2527141/150‑1 (MQ=255)
                                                                                         tctGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATg                                                             <  1:4324254/150‑1 (MQ=255)
                                                                                         tctGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATg                                                             <  1:77318/150‑1 (MQ=255)
                                                                                             cggcgCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCAGTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTg                                                         <  2:2655951/150‑1 (MQ=255)
                                                                                                 gcgcCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCCACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCt                                                     <  2:111529/150‑1 (MQ=255)
                                                                                                   gcCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTaa                                                   <  2:88190/150‑1 (MQ=255)
                                                                                                     cTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAAc                                                 >  2:100329/1‑150 (MQ=255)
                                                                                                      ttCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAAcc                                                <  2:3203237/150‑1 (MQ=255)
                                                                                                      ttCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAAcc                                                >  2:2295108/1‑150 (MQ=255)
                                                                                                         tGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCt                                             <  1:1368764/150‑1 (MQ=255)
                                                                                                               gATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTccc                                       >  2:3051697/1‑150 (MQ=255)
                                                                                                                aTACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCa                                      >  2:1786620/1‑150 (MQ=255)
                                                                                                                   cccACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCg                                   >  2:3769948/1‑150 (MQ=255)
                                                                                                                     cACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGt                                 >  1:1197155/1‑150 (MQ=255)
                                                                                                                     cACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGt                                 >  1:3188782/1‑150 (MQ=255)
                                                                                                                        cTGCATGAAGTCCACCTCAACACCCTTTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAAc                              <  1:3543317/150‑1 (MQ=255)
                                                                                                                          gCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACaa                            >  1:1899167/1‑150 (MQ=255)
                                                                                                                          gCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACaa                            <  2:2706443/150‑1 (MQ=255)
                                                                                                                           cATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAAt                           >  1:8456/1‑150 (MQ=255)
                                                                                                                            aTGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAAtt                          >  2:1550430/1‑150 (MQ=255)
                                                                                                                             tGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTg                         <  1:384546/150‑1 (MQ=255)
                                                                                                                                 gTCCACCTCAACACCCTGTTATTCAATCATTTCCTTAGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGt                     <  1:2907607/150‑1 (MQ=255)
                                                                                                                                  tCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGtt                    <  1:477347/150‑1 (MQ=255)
                                                                                                                                     aCCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTa                 <  2:3094708/150‑1 (MQ=255)
                                                                                                                                      ccTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc                >  1:2339013/1‑150 (MQ=255)
                                                                                                                                       cTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAcc               <  2:6503/150‑1 (MQ=255)
                                                                                                                                       cTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAAGGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAcc               <  2:2053539/150‑1 (MQ=255)
                                                                                                                                         cAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCtt             >  1:80723/1‑150 (MQ=255)
                                                                                                                                         cAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCtt             >  2:4098486/1‑150 (MQ=255)
                                                                                                                                               ccTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAggg       <  2:853023/150‑1 (MQ=255)
                                                                                                                                                cTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGga      <  1:934036/150‑1 (MQ=255)
                                                                                                                                                 tGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGgag     <  1:3946184/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAA  >  CP009273/2893297‑2893594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑