BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,927,618	C→A	8.8%	Q9K (CAG→AAG)	fucP →	L‑fucose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,927,618	0	C	A	8.8%	1319.6 / 59.0	353	Q9K (CAG→AAG)	fucP	L‑fucose transporter
Reads supporting (aligned to +/- strand): ref base C (176/144); new base A (16/15); total (192/161)
Fisher's exact test for biased strand distribution p-value = 8.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 355 total aligned reads displayed.

ATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACATTTTATTACCTCAATTCCAGCAGGCTTTTACGCTG  >  CP009273/2927470‑2927767

aTGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCa                                                                                                                                                      <  2:1124468/150‑1 (MQ=255)

  gAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCaga                                                                                                                                                    <  1:4596211/150‑1 (MQ=255)

   aaTCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCagag                                                                                                                                                   <  1:1954456/150‑1 (MQ=255)

     tCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGtt                                                                                                                                                 >  2:27077/1‑150 (MQ=255)

     tCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGtt                                                                                                                                                 <  1:3764301/150‑1 (MQ=255)

      ccATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTa                                                                                                                                                <  2:2413158/150‑1 (MQ=255)

        aTGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTAcc                                                                                                                                              >  2:2149932/1‑150 (MQ=255)

         tGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCg                                                                                                                                             <  1:3530546/150‑1 (MQ=255)

         tGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCg                                                                                                                                             >  1:4264477/1‑150 (MQ=255)

          gAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGAAGAGTTACCGt                                                                                                                                            <  1:3504850/150‑1 (MQ=255)

           aGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTg                                                                                                                                           >  2:3719652/1‑150 (MQ=255)

           aGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTg                                                                                                                                           >  1:3968247/1‑150 (MQ=255)

             ttCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCg                                                                                                                                         >  1:252978/1‑150 (MQ=255)

               cATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGt                                                                                                                                       >  2:2879052/1‑150 (MQ=255)

                aTTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGATTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCTTGCGGTa                                                                                                                                      >  1:324806/1‑150 (MQ=255)

                   tCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGAt                                                                                                                                   >  1:1178389/1‑150 (MQ=255)

                          ggCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATg                                                                                                                            <  2:2328128/150‑1 (MQ=255)

                             aaaTATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGAAGAGTTACCGTGCGGTAGATAAAGATGCAg                                                                                                                         <  1:258616/150‑1 (MQ=255)

                               atatTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAggg                                                                                                                       <  1:4033531/150‑1 (MQ=255)

                                 atTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCGGGGCa                                                                                                                     <  1:679679/150‑1 (MQ=255)

                                    cACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAg                                                                                                                  >  2:1953862/1‑150 (MQ=255)

                                     aCTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGc                                                                                                                 <  2:706501/150‑1 (MQ=255)

                                      cTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCa                                                                                                                <  1:2913015/150‑1 (MQ=255)

                                      cTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCa                                                                                                                >  2:1548520/1‑150 (MQ=255)

                                       tGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAg                                                                                                               >  2:135577/1‑150 (MQ=255)

                                        gATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGAAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGa                                                                                                              >  1:1336028/1‑150 (MQ=255)

                                         atatGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGaa                                                                                                             <  2:3293869/150‑1 (MQ=255)

                                         atatGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAAAACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGaa                                                                                                             >  2:127009/1‑150 (MQ=255)

                                            tGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGtt                                                                                                          <  1:2331577/150‑1 (MQ=255)

                                            tGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGtt                                                                                                          <  1:121061/150‑1 (MQ=255)

                                              aaGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTAc                                                                                                        <  1:2136134/150‑1 (MQ=255)

                                              aaGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGGTAc                                                                                                        >  1:4179807/1‑150 (MQ=255)

                                              aaGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGAAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTAc                                                                                                        <  2:850108/150‑1 (MQ=255)

                                                 cccGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACatt                                                                                                     <  2:3831395/150‑1 (MQ=255)

                                                  ccGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACatta                                                                                                    <  1:1230203/150‑1 (MQ=255)

                                                  ccGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGAAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACatta                                                                                                    >  2:3200323/1‑150 (MQ=255)

                                                   cGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACattat                                                                                                   >  1:3582245/1‑150 (MQ=255)

                                                   cGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGAAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACattat                                                                                                   >  1:4504224/1‑150 (MQ=255)

                                                    gAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACattatt                                                                                                  >  2:2090784/1‑150 (MQ=255)

                                                    gAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACattatt                                                                                                  <  2:457185/150‑1 (MQ=255)

                                                     aaCTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTc                                                                                                 >  2:281633/1‑150 (MQ=255)

                                                      aCTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTcc                                                                                                <  1:3723964/150‑1 (MQ=255)

                                                      aCTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTcc                                                                                                <  1:3161048/150‑1 (MQ=255)

                                                      aCTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTcc                                                                                                >  2:4040943/1‑150 (MQ=255)

                                                        tCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCAt                                                                                              >  1:699058/1‑150 (MQ=255)

                                                          gCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTc                                                                                            >  2:446663/1‑150 (MQ=255)

                                                          gCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTc                                                                                            >  2:1169440/1‑150 (MQ=255)

                                                           cTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTcg                                                                                           <  2:2255122/150‑1 (MQ=255)

                                                            tGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTcgc                                                                                          <  1:3085758/150‑1 (MQ=255)

                                                              gTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTAGGGGAAACACATCAATACAAACGCAGAGTTACCATGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCgcgc                                                                                        >  1:1012706/1‑150 (MQ=255)

                                                                tttGCACTTTTGAAAACATAACCGATTAAGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCgctg                                                                                      >  2:1594772/1‑150 (MQ=255)

                                                                   gCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGctgctg                                                                                   >  2:2745845/1‑150 (MQ=255)

                                                                       ttttGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCt                                                                               >  1:3932499/1‑150 (MQ=255)

                                                                          tGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCAc                                                                            >  1:1281501/1‑150 (MQ=255)

                                                                           gAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACt                                                                           >  1:179640/1‑150 (MQ=255)

                                                                            aaaaCATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTg                                                                          >  2:3596006/1‑150 (MQ=255)

                                                                            aaaaCATAACCGATGACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTg                                                                          <  1:3480850/150‑1 (MQ=255)

                                                                             aaaCATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGt                                                                         >  1:534923/1‑150 (MQ=255)

                                                                               aCATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGttt                                                                       >  2:4104207/1‑150 (MQ=255)

                                                                                 aTAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGttttt                                                                     >  2:4011094/1‑150 (MQ=255)

                                                                                 aTAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGttttt                                                                     >  2:3415456/1‑150 (MQ=255)

                                                                                  tAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGAAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGtttttt                                                                    >  2:174694/1‑150 (MQ=255)

                                                                                    aCCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCt                                                                  <  2:4404049/150‑1 (MQ=255)

                                                                                    aCCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCt                                                                  >  2:2954466/1‑150 (MQ=255)

                                                                                     ccGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCtt                                                                 >  1:1088696/1‑150 (MQ=255)

                                                                                       gATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTg                                                               >  1:867880/1‑150 (MQ=255)

                                                                                          tACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGc                                                            >  2:1431574/1‑150 (MQ=255)

                                                                                          tACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGc                                                            <  2:3095620/150‑1 (MQ=255)

                                                                                          tACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGAAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGc                                                            >  2:543738/1‑150 (MQ=255)

                                                                                             gTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGt                                                         >  1:2170190/1‑150 (MQ=255)

                                                                                              tGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTa                                                        >  1:2394174/1‑150 (MQ=255)

                                                                                                     gCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATa                                                 >  1:1456907/1‑150 (MQ=255)

                                                                                                      cTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATaa                                                <  1:1399548/150‑1 (MQ=255)

                                                                                                        tCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAAcc                                              >  2:604062/1‑150 (MQ=255)

                                                                                                        tCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAAcc                                              <  2:4584682/150‑1 (MQ=255)

                                                                                                        tCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAAcc                                              >  1:4221787/1‑150 (MQ=255)

                                                                                                         cTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTAGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCt                                             >  2:2064450/1‑150 (MQ=255)

                                                                                                          tGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCtt                                            <  1:1339292/150‑1 (MQ=255)

                                                                                                          tGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCtt                                            >  1:2033358/1‑150 (MQ=255)

                                                                                                          tGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCtt                                            <  2:3251803/150‑1 (MQ=255)

                                                                                                              cccaaGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACg                                        <  1:794741/147‑1 (MQ=255)

                                                                                                              ccTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACg                                        >  2:936794/1‑150 (MQ=255)

                                                                                                              ccTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACg                                        >  2:2437465/1‑150 (MQ=255)

                                                                                                                 aaGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACa                                     >  1:3336361/1‑150 (MQ=255)

                                                                                                                  agagGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACAt                                    <  1:742855/150‑1 (MQ=255)

                                                                                                                  agagGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACAt                                    >  1:2280156/1‑150 (MQ=255)

                                                                                                                   gagGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACAtt                                   <  2:1317020/150‑1 (MQ=255)

                                                                                                                    agGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACAttt                                  <  1:3295921/150‑1 (MQ=255)

                                                                                                                     ggATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACAtttt                                 >  1:185476/1‑150 (MQ=255)

                                                                                                                          cTATGGGAAACACATCAATACAAACGAAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACTTTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACATTTTATTAc                            <  1:4542712/150‑1 (MQ=255)

                                                                                                                              gggAAACACATCAATACAAACGAAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACATTTTATTACCTCa                        <  1:1692214/150‑1 (MQ=255)

                                                                                                                                gAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACATTTTATTACCTCAAt                      >  1:3102387/1‑150 (MQ=255)

                                                                                                                                 aaaCACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACATTTTATTACCTCAAtt                     <  1:4080750/150‑1 (MQ=255)

                                                                                                                                   acacATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACATTTTATTACCTCAATTcc                   <  2:335303/150‑1 (MQ=255)

                                                                                                                                          aaTACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACATTTTATTACCTCAATTCCAGCAGGc            >  1:4361527/1‑150 (MQ=255)

                                                                                                                                              cAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACATTTTATTACCTCAATTCCAGCAGGCtttt        <  1:4385988/150‑1 (MQ=255)

                                                                                                                                               aaaCGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACATTTTATTACCTCAATTCCAGCAGGCTTTTa       <  2:2573551/150‑1 (MQ=255)

                                                                                                                                                 aCGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACATTTTATTACCTCAATTCCAGCAGGCTTTTACg     >  1:3033365/1‑150 (MQ=255)

                                                                                                                                                  cGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACATTTTATTACCTCAATTCCAGCAGGCTTTTACGc    >  2:4240647/1‑150 (MQ=255)

                                                                                                                                                  cGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACATTTTATTACCTCAATTCCAGCAGGCTTTTACGc    <  1:2677894/150‑1 (MQ=255)

ATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTTTTTTCTTTGGGCGGTAGCCAATAACCTTAACGACATTTTATTACCTCAATTCCAGCAGGCTTTTACGCTG  >  CP009273/2927470‑2927767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`