Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,993,211 C→A 100% V14L (GTG→TTG)  ygeR ← LysM domain‑containing M23 family putative peptidase; septation lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,993,2110CA97.8% 893.8 / 10.1 267V14L (GTG→TTG) ygeRLysM domain‑containing M23 family putative peptidase; septation lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (5/1);  new base A (122/138);  total (128/139)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 268 total aligned reads displayed.

CTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCAT  >  CP009273/2993064‑2993360
                                                                                                                                                   |                                                                                                                                                     
cTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGa                                                                                                                                                     >  2:879545/1‑150 (MQ=255)
cTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGa                                                                                                                                                     >  2:3523085/1‑150 (MQ=255)
cTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGa                                                                                                                                                     <  1:2465920/150‑1 (MQ=255)
 ttttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGAt                                                                                                                                                    <  2:1489191/150‑1 (MQ=255)
 ttttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGAt                                                                                                                                                    >  1:3234733/1‑150 (MQ=255)
   ttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATAc                                                                                                                                                  >  1:2378434/1‑150 (MQ=255)
    tACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATAcc                                                                                                                                                 <  2:1448863/150‑1 (MQ=255)
    tACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATAcc                                                                                                                                                 <  2:2752552/150‑1 (MQ=255)
     aCGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATAccc                                                                                                                                                >  2:2297971/1‑150 (MQ=255)
     aCGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATAccc                                                                                                                                                <  1:4232389/150‑1 (MQ=255)
            ttCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAtt                                                                                                                                         >  2:1685709/1‑150 (MQ=255)
             tCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAttt                                                                                                                                        <  2:1262902/150‑1 (MQ=255)
             tCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAttt                                                                                                                                        >  2:3659565/1‑150 (MQ=255)
             tCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAttt                                                                                                                                        >  2:50685/1‑150 (MQ=255)
               ccGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAttttt                                                                                                                                      >  2:2111647/1‑150 (MQ=255)
                cGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAtttttt                                                                                                                                     >  1:3473142/1‑150 (MQ=255)
                 gtggtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTa                                                                                                                                    >  1:1149233/1‑150 (MQ=255)
                    gtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTc                                                                                                                                 >  1:222710/1‑150 (MQ=255)
                     tgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCa                                                                                                                                <  2:3540583/150‑1 (MQ=255)
                      gcgcGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAg                                                                                                                               <  1:2793289/150‑1 (MQ=255)
                      gcgcGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAg                                                                                                                               <  2:4560239/150‑1 (MQ=255)
                         cgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCg                                                                                                                            >  2:1508677/1‑150 (MQ=255)
                         cgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCg                                                                                                                            >  2:1119298/1‑150 (MQ=255)
                         cgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCg                                                                                                                            >  2:1294914/1‑150 (MQ=255)
                         cgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCg                                                                                                                            >  1:1141421/1‑150 (MQ=255)
                           aaaTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTc                                                                                                                          >  2:73638/1‑150 (MQ=255)
                            aaTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTcc                                                                                                                         >  1:2568538/1‑150 (MQ=255)
                                cGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCa                                                                                                                     <  2:3815097/150‑1 (MQ=255)
                                 gATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCAc                                                                                                                    >  1:1895464/1‑150 (MQ=255)
                                 gATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCAc                                                                                                                    >  2:59254/1‑150 (MQ=255)
                                  atctaGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACt                                                                                                                   <  1:1372806/147‑1 (MQ=255)
                                    ataGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCa                                                                                                                 >  1:2603894/1‑150 (MQ=255)
                                      aGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCaaa                                                                                                               >  1:4437380/1‑150 (MQ=255)
                                          tATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCt                                                                                                           >  1:3401322/1‑150 (MQ=255)
                                          tATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCt                                                                                                           <  1:1620096/150‑1 (MQ=255)
                                          tATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCt                                                                                                           <  2:1932614/150‑1 (MQ=255)
                                            tCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTACCTCTATCGGATGAATTGCTACACCAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGAAACCCCGAGATTATTTATTCAGGCGACCCGCACTCAAAATCttt                                                                                                         >  2:146355/1‑150 (MQ=255)
                                              cccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTcc                                                                                                       >  2:3042838/1‑150 (MQ=255)
                                                   gTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCt                                                                                                  >  2:2598198/1‑150 (MQ=255)
                                                   gTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCt                                                                                                  >  2:2496554/1‑150 (MQ=255)
                                                    tttCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCtt                                                                                                 <  1:407834/150‑1 (MQ=255)
                                                         cGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTaaaaa                                                                                            <  1:555514/150‑1 (MQ=255)
                                                            tgtAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACa                                                                                         <  1:1428034/150‑1 (MQ=255)
                                                             gtAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACaa                                                                                        >  1:3807954/1‑150 (MQ=255)
                                                             gtAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACaa                                                                                        >  2:203943/1‑150 (MQ=255)
                                                             gtAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACaa                                                                                        >  2:2936315/1‑150 (MQ=255)
                                                              tAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTTAAAAACAAg                                                                                       <  2:70678/150‑1 (MQ=255)
                                                                   ggAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGcc                                                                                  >  2:2704214/1‑150 (MQ=255)
                                                                   ggAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGcc                                                                                  >  2:971700/1‑150 (MQ=255)
                                                                    gAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCt                                                                                 >  2:3150642/1‑150 (MQ=255)
                                                                     aGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCtt                                                                                <  2:4587248/150‑1 (MQ=255)
                                                                      gCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTa                                                                               >  1:1766565/1‑150 (MQ=255)
                                                                      gCCGGAATAAGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATAACCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTa                                                                               <  1:961806/150‑1 (MQ=255)
                                                                       ccGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTAt                                                                              <  1:879770/150‑1 (MQ=255)
                                                                       ccGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTAt                                                                              <  1:3523109/150‑1 (MQ=255)
                                                                         ggAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCa                                                                            <  2:1617931/150‑1 (MQ=255)
                                                                         ggAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCa                                                                            >  1:1039997/1‑150 (MQ=255)
                                                                         ggAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCa                                                                            >  1:2216404/1‑150 (MQ=255)
                                                                           aaTACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATa                                                                          >  1:3097682/1‑150 (MQ=255)
                                                                            aTACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATag                                                                         <  1:3634602/150‑1 (MQ=255)
                                                                               cGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATagcag                                                                      <  1:447384/150‑1 (MQ=255)
                                                                                gTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATagcagc                                                                     >  2:376393/1‑150 (MQ=255)
                                                                                gTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATagcagc                                                                     >  1:2916885/1‑150 (MQ=255)
                                                                                        aTCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGGTACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCctct                                                             >  1:672461/1‑150 (MQ=255)
                                                                                        aTCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCctct                                                             >  1:1100772/1‑150 (MQ=255)
                                                                                         tCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCtt                                                            <  2:225846/150‑1 (MQ=255)
                                                                                         tCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCtt                                                            <  2:2444319/150‑1 (MQ=255)
                                                                                          cGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTg                                                           <  1:4252260/150‑1 (MQ=255)
                                                                                            gatgatTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGAt                                                         <  2:842866/150‑1 (MQ=255)
                                                                                              tgatTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATAc                                                       <  2:220970/150‑1 (MQ=255)
                                                                                                   tGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCATGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAAt                                                  <  2:1482342/150‑1 (MQ=255)
                                                                                                    gCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAAtc                                                 <  1:1005854/150‑1 (MQ=255)
                                                                                                          cGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGc                                           <  2:1227053/150‑1 (MQ=255)
                                                                                                          cGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGc                                           <  1:3216618/150‑1 (MQ=255)
                                                                                                               aGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTg                                      <  2:1707785/150‑1 (MQ=255)
                                                                                                                 cccGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGa                                    <  2:3293656/150‑1 (MQ=255)
                                                                                                                   cGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGaaa                                  <  2:1083917/150‑1 (MQ=255)
                                                                                                                    gCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGaaaa                                 >  2:790706/1‑150 (MQ=255)
                                                                                                                      cAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAAc                               <  1:159652/150‑1 (MQ=255)
                                                                                                                          aGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTgg                           >  2:2503977/1‑150 (MQ=255)
                                                                                                                              gTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAAt                       >  2:2643812/1‑150 (MQ=255)
                                                                                                                               tCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATa                      <  1:3083171/150‑1 (MQ=255)
                                                                                                                               tCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATa                      <  1:2129828/150‑1 (MQ=255)
                                                                                                                               tCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCGTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACTTGGTAATa                      <  2:882144/150‑1 (MQ=255)
                                                                                                                                ccAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATag                     >  2:2497090/1‑150 (MQ=255)
                                                                                                                                 cAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATaga                    >  2:4303065/1‑150 (MQ=255)
                                                                                                                                   aCCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAgaga                  <  1:3901652/150‑1 (MQ=255)
                                                                                                                                     cGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGaaa                <  2:3292796/150‑1 (MQ=255)
                                                                                                                                     cGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGaaa                >  1:2903738/1‑150 (MQ=255)
                                                                                                                                     cGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGaaa                >  1:3477303/1‑150 (MQ=255)
                                                                                                                                      gATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAag               >  1:2707417/1‑150 (MQ=255)
                                                                                                                                       aTAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAaga              <  2:4370942/150‑1 (MQ=255)
                                                                                                                                         aacaacATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAgg            >  1:4606749/1‑150 (MQ=255)
                                                                                                                                         aacaacATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAgg            <  1:4561240/150‑1 (MQ=255)
                                                                                                                                         aacaacATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAgg            >  2:368435/1‑150 (MQ=255)
                                                                                                                                          acaacaTCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGt           >  1:1234391/1‑150 (MQ=255)
                                                                                                                                            aacaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTa         >  2:4162308/1‑150 (MQ=255)
                                                                                                                                            aacaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTa         <  1:3920524/150‑1 (MQ=255)
                                                                                                                                                tCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAAttt     >  1:869296/1‑150 (MQ=255)
                                                                                                                                                   cGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCAt  <  1:3794394/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCAT  >  CP009273/2993064‑2993360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑