BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,021,141	T→C	12.2%	I221T (ATC→ACC)	ygfQ →	putative purine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,021,141	0	T	C	12.2%	705.1 / 76.1	248	I221T (ATC→ACC)	ygfQ	putative purine permease
Reads supporting (aligned to +/- strand): ref base T (114/103); new base C (8/22); total (123/125)
Fisher's exact test for biased strand distribution p-value = 1.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

CAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGG  >  CP009273/3020992‑3021290

cAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGat                                                                                                                                                       <  2:1797408/150‑1 (MQ=255)

cAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGat                                                                                                                                                       >  2:4167593/1‑150 (MQ=255)

 aaaaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGatc                                                                                                                                                      >  1:3319532/1‑150 (MQ=255)

 aaaaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGatc                                                                                                                                                      <  1:509793/150‑1 (MQ=255)

  aaaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGatca                                                                                                                                                     >  1:2372835/1‑150 (MQ=255)

  aaaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTGGGTGATTATTGCAATTTCGatca                                                                                                                                                     >  2:77155/1‑150 (MQ=255)

    aaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGatcatc                                                                                                                                                   >  2:3958121/1‑150 (MQ=255)

    aaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGatcatc                                                                                                                                                   <  1:2330891/150‑1 (MQ=255)

       ccGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGc                                                                                                                                                >  2:2962254/1‑150 (MQ=255)

         gATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCtt                                                                                                                                              <  2:4555878/150‑1 (MQ=255)

          aTTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTa                                                                                                                                             >  1:1512510/1‑150 (MQ=255)

          aTTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTa                                                                                                                                             <  1:79189/150‑1 (MQ=255)

          aTTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTa                                                                                                                                             <  1:951027/150‑1 (MQ=255)

             gAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATc                                                                                                                                          >  1:1611046/1‑150 (MQ=255)

               aGGCTTGCCAGTGGCGCTCGGTGCGTGTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCtt                                                                                                                                        <  2:4321637/150‑1 (MQ=255)

                ggCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCttt                                                                                                                                       <  2:4417115/150‑1 (MQ=255)

                 gCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTg                                                                                                                                      >  1:2462196/1‑150 (MQ=255)

                   ttGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGAc                                                                                                                                    >  1:1419559/1‑150 (MQ=255)

                    tGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGAcc                                                                                                                                   >  1:641468/1‑150 (MQ=255)

                      ccAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCa                                                                                                                                 <  1:1765571/150‑1 (MQ=255)

                      ccAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCa                                                                                                                                 <  2:185860/150‑1 (MQ=255)

                        aGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGc                                                                                                                               >  1:4070702/1‑150 (MQ=255)

                         gTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCg                                                                                                                              <  2:2839098/150‑1 (MQ=255)

                          tGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCgg                                                                                                                             >  2:699609/1‑150 (MQ=255)

                           ggCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGt                                                                                                                            <  2:95269/150‑1 (MQ=255)

                               cTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGaaa                                                                                                                        >  2:2027551/1‑150 (MQ=255)

                                   gTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATAcc                                                                                                                    >  1:3401712/1‑150 (MQ=255)

                                   gTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATAcc                                                                                                                    >  1:4342699/1‑150 (MQ=255)

                                   gTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATAcc                                                                                                                    >  1:2923405/1‑150 (MQ=255)

                                   gTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATAcc                                                                                                                    >  1:2072956/1‑150 (MQ=255)

                                          tACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCt                                                                                                             <  2:4203212/150‑1 (MQ=255)

                                                ctTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGc                                                                                                       >  1:3378132/1‑150 (MQ=255)

                                                 ttCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCg                                                                                                      >  1:2807195/1‑150 (MQ=255)

                                                    ccGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATg                                                                                                   <  2:4625411/150‑1 (MQ=255)

                                                      ggTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGcc                                                                                                 <  2:2327675/150‑1 (MQ=255)

                                                      ggTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGcc                                                                                                 >  2:2023399/1‑150 (MQ=255)

                                                       gTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCa                                                                                                <  1:3442142/150‑1 (MQ=255)

                                                            gatgaGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCt                                                                                           <  1:2407811/150‑1 (MQ=255)

                                                             atgaGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTg                                                                                          <  1:1336034/150‑1 (MQ=255)

                                                               gaGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGAc                                                                                        <  1:3092125/150‑1 (MQ=255)

                                                                aGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACt                                                                                       >  2:4057036/1‑150 (MQ=255)

                                                                aGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATAACACGGTCTGGTGGCGATGCCAATCCTGACt                                                                                       >  1:1155570/1‑150 (MQ=255)

                                                                aGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACt                                                                                       <  2:2288770/150‑1 (MQ=255)

                                                                aGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACt                                                                                       <  2:2288466/150‑1 (MQ=255)

                                                                    tGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCg                                                                                   >  1:4074043/1‑150 (MQ=255)

                                                                       tGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAg                                                                                >  1:2892453/1‑150 (MQ=255)

                                                                       tGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAg                                                                                >  1:2892635/1‑150 (MQ=255)

                                                                         gggCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGAt                                                                              <  1:2136676/150‑1 (MQ=255)

                                                                         gggCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGAt                                                                              <  1:3000474/150‑1 (MQ=255)

                                                                          ggCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATg                                                                             <  1:3608541/150‑1 (MQ=255)

                                                                           gCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATgg                                                                            <  1:4480534/150‑1 (MQ=255)

                                                                              ggCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTaa                                                                         <  2:139264/150‑1 (MQ=255)

                                                                                 ggTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGtc                                                                      >  2:2098746/1‑150 (MQ=255)

                                                                                    cATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTctct                                                                   <  2:3850806/150‑1 (MQ=255)

                                                                                     aTCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTg                                                                  >  1:3221688/1‑150 (MQ=255)

                                                                                     aTCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTg                                                                  <  1:1208367/150‑1 (MQ=255)

                                                                                       cTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGAt                                                                >  1:1948896/1‑150 (MQ=255)

                                                                                        ttCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGAtt                                                               <  2:1676608/150‑1 (MQ=255)

                                                                                            gCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCa                                                           >  1:1851928/1‑150 (MQ=255)

                                                                                            gCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCa                                                           <  2:3815111/150‑1 (MQ=255)

                                                                                             ccTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAg                                                          >  1:885236/1‑150 (MQ=255)

                                                                                              cTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGc                                                         >  2:2607411/1‑150 (MQ=255)

                                                                                              cTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATGTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGc                                                         <  1:3715360/150‑1 (MQ=255)

                                                                                                  agaAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCg                                                     <  1:1649447/150‑1 (MQ=255)

                                                                                                  agaAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCg                                                     <  2:3624317/150‑1 (MQ=255)

                                                                                                  agaAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCg                                                     <  2:3624282/150‑1 (MQ=255)

                                                                                                  agaAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATAGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCg                                                     <  1:3165820/150‑1 (MQ=255)

                                                                                                   gaAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGAGTTTCAGCCTCGa                                                    >  1:1080875/1‑150 (MQ=255)

                                                                                                    aaGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGat                                                   <  2:640711/150‑1 (MQ=255)

                                                                                                      gtgtCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGatat                                                 <  2:1982655/150‑1 (MQ=255)

                                                                                                      gtgtCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGatat                                                 <  2:3506227/150‑1 (MQ=255)

                                                                                                         tCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGAtattat                                              <  2:2208279/150‑1 (MQ=255)

                                                                                                         tCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGAtattat                                              <  2:2364097/150‑1 (MQ=255)

                                                                                                               aCCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGc                                        <  1:4516486/150‑1 (MQ=255)

                                                                                                               aCCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGc                                        >  2:3827317/1‑150 (MQ=255)

                                                                                                                   ggcggGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTc                                    >  1:3649195/1‑150 (MQ=255)

                                                                                                                    gcggGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTcc                                   <  2:3218867/150‑1 (MQ=255)

                                                                                                                     cggGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCa                                  >  2:2803963/1‑150 (MQ=255)

                                                                                                                      gggATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAg                                 >  1:1509038/1‑150 (MQ=255)

                                                                                                                      gggATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAg                                 >  1:1965580/1‑150 (MQ=255)

                                                                                                                             tgttgGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTg                          >  1:4007599/1‑150 (MQ=255)

                                                                                                                              gttgGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGt                         <  2:74002/150‑1 (MQ=255)

                                                                                                                               ttgGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTa                        >  2:4359417/1‑150 (MQ=255)

                                                                                                                                  gTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTATCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACtt                     >  2:4121343/1‑150 (MQ=255)

                                                                                                                                   tGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTc                    <  2:3806215/150‑1 (MQ=255)

                                                                                                                                    gATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTcc                   >  2:2700093/1‑150 (MQ=255)

                                                                                                                                     attattGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCg                  >  2:89857/1‑150 (MQ=255)

                                                                                                                                     attattGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCg                  <  1:3832567/150‑1 (MQ=255)

                                                                                                                                     attattGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCg                  >  1:698602/1‑150 (MQ=255)

                                                                                                                                      ttattGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGa                 <  1:2552202/150‑1 (MQ=255)

                                                                                                                                      ttattGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGa                 <  1:2629250/150‑1 (MQ=255)

                                                                                                                                      ttattGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGa                 <  2:3378132/150‑1 (MQ=255)

                                                                                                                                       tattGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAg                >  2:2603762/1‑150 (MQ=255)

                                                                                                                                         ttGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAgtg              >  2:899909/1‑150 (MQ=255)

                                                                                                                                             aaTTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACt          >  2:3147531/1‑150 (MQ=255)

                                                                                                                                             aaTTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACt          >  1:471574/1‑150 (MQ=255)

                                                                                                                                             aaTTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACt          <  1:4255015/150‑1 (MQ=255)

                                                                                                                                               tttCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTgg        >  2:3486962/1‑150 (MQ=255)

                                                                                                                                                  cGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCAt     >  1:4295798/1‑150 (MQ=255)

                                                                                                                                                    atcatcGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTg   >  2:1530137/1‑150 (MQ=255)

CAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGG  >  CP009273/3020992‑3021290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`