Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,048,490 A→T 14.7% L105* (TTG→TAG)  ygfB ← UPF0149 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,048,4900AT14.7% 674.9 / 93.9 237L105* (TTG→TAG) ygfBUPF0149 family protein
Reads supporting (aligned to +/- strand):  ref base A (117/85);  new base T (11/24);  total (128/109)
Fisher's exact test for biased strand distribution p-value = 5.35e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

CAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCG  >  CP009273/3048341‑3048639
                                                                                                                                                     |                                                                                                                                                     
cAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCa                                                                                                                                                       <  2:3983316/150‑1 (MQ=255)
  aGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCTAt                                                                                                                                                     <  1:357417/150‑1 (MQ=255)
    ctctTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGc                                                                                                                                                   <  1:1677290/150‑1 (MQ=255)
     tctTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTTACCCAACCTGCCAATGCa                                                                                                                                                  >  1:1730702/1‑150 (MQ=255)
        tCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCTATGCATCa                                                                                                                                               <  1:1558806/150‑1 (MQ=255)
           tGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCTATGCATCAGcc                                                                                                                                            <  2:1957100/150‑1 (MQ=255)
           tGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGcc                                                                                                                                            <  2:345034/150‑1 (MQ=255)
            gATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGccc                                                                                                                                           >  1:3387794/1‑150 (MQ=255)
             aTCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCg                                                                                                                                          >  1:1441459/1‑150 (MQ=255)
              tcttcgtcttcgTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGa                                                                                                                                         <  2:4028244/150‑1 (MQ=255)
              tcttcgtcttcgTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGa                                                                                                                                         <  2:4369941/150‑1 (MQ=255)
                ttcgtcttcgtcGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGCAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATc                                                                                                                                       <  1:1799790/150‑1 (MQ=255)
                 tcgtcttcgtcGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCTATGCATCAGCCCGATCg                                                                                                                                      <  1:2300880/150‑1 (MQ=255)
                 tcgtcttcgtcGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCg                                                                                                                                      <  1:1884731/150‑1 (MQ=255)
                  cgtcttcgtcGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGa                                                                                                                                     >  2:4218247/1‑150 (MQ=255)
                  cgtcttcgtcGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGa                                                                                                                                     >  2:2805731/1‑150 (MQ=255)
                   gtcttcgtcGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGaa                                                                                                                                    <  1:2017815/150‑1 (MQ=255)
                    tcttcgtcGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGaaa                                                                                                                                   <  2:3365958/150‑1 (MQ=255)
                    tcttcgtcGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGaaa                                                                                                                                   >  2:4007989/1‑150 (MQ=255)
                        cgtcgtAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGc                                                                                                                               >  1:4036247/1‑150 (MQ=255)
                        cgtcgtAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGc                                                                                                                               >  1:1111934/1‑150 (MQ=255)
                         gtcgtAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCt                                                                                                                              <  1:4424481/150‑1 (MQ=255)
                          tcgtAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTg                                                                                                                             <  2:828200/150‑1 (MQ=255)
                            gtAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGAc                                                                                                                           <  2:4498760/150‑1 (MQ=255)
                            gtAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGAc                                                                                                                           >  2:3209852/1‑150 (MQ=255)
                             tAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGAca                                                                                                                          <  2:3831649/150‑1 (MQ=255)
                             tAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGAca                                                                                                                          <  2:3831550/150‑1 (MQ=255)
                              aaCCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAATATATACAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACACAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGAcat                                                                                                                         >  1:2088348/1‑150 (MQ=255)
                                   aGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCg                                                                                                                    >  1:3311589/1‑150 (MQ=255)
                                     ttGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGcc                                                                                                                  <  2:1729770/150‑1 (MQ=255)
                                        cgcAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATc                                                                                                               <  2:4397246/150‑1 (MQ=255)
                                         gcAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCa                                                                                                              <  2:1649578/150‑1 (MQ=255)
                                          cAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAg                                                                                                             >  1:479294/1‑150 (MQ=255)
                                            aTGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGc                                                                                                           <  2:3701349/150‑1 (MQ=255)
                                            aTGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGc                                                                                                           <  2:4542681/150‑1 (MQ=255)
                                             tGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCa                                                                                                          <  1:598786/150‑1 (MQ=255)
                                             tGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCa                                                                                                          >  2:719633/1‑150 (MQ=255)
                                               ttACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGa                                                                                                        <  2:1808084/150‑1 (MQ=255)
                                                 aCGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATa                                                                                                      <  1:2987624/150‑1 (MQ=255)
                                                   gCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATaaa                                                                                                    >  2:1784783/1‑150 (MQ=255)
                                                   gCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATaaa                                                                                                    >  2:1396754/1‑150 (MQ=255)
                                                      gATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCt                                                                                                 >  2:3578406/1‑150 (MQ=255)
                                                         cgtcgATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGaa                                                                                              <  1:2883947/150‑1 (MQ=255)
                                                         cgtcgATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGaa                                                                                              >  2:3882872/1‑150 (MQ=255)
                                                               tAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGa                                                                                        <  1:904125/150‑1 (MQ=255)
                                                                aGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGaa                                                                                       >  1:4001670/1‑150 (MQ=255)
                                                                aGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGaa                                                                                       >  1:1300769/1‑150 (MQ=255)
                                                                   ttCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGcc                                                                                    >  2:2284150/1‑150 (MQ=255)
                                                                   ttCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGcc                                                                                    <  2:2465033/150‑1 (MQ=255)
                                                                   ttCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGcc                                                                                    >  1:3123670/1‑150 (MQ=255)
                                                                     cACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGt                                                                                  >  2:2264467/1‑150 (MQ=255)
                                                                         ggTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATc                                                                              >  2:3195893/1‑150 (MQ=255)
                                                                         ggTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATc                                                                              >  2:140135/1‑150 (MQ=255)
                                                                              cGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCa                                                                         >  1:3458275/1‑150 (MQ=255)
                                                                               gCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAg                                                                        >  1:1484729/1‑150 (MQ=255)
                                                                                  ggTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGc                                                                     <  1:3435971/150‑1 (MQ=255)
                                                                                    tCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCTATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCAt                                                                   >  1:506598/1‑150 (MQ=255)
                                                                                     cACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATc                                                                  >  2:1736093/1‑150 (MQ=255)
                                                                                        tttATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCt                                                               <  1:820382/150‑1 (MQ=255)
                                                                                        tttATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCt                                                               >  1:1718175/1‑150 (MQ=255)
                                                                                          tATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCtgg                                                             >  2:1568895/1‑150 (MQ=255)
                                                                                            tCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCTATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCtggtg                                                           <  2:4230040/150‑1 (MQ=255)
                                                                                            tCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCTATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCtggtg                                                           <  2:4230047/150‑1 (MQ=255)
                                                                                               aGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCTATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCa                                                        <  1:3314770/150‑1 (MQ=255)
                                                                                                   tCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGt                                                    <  1:3007375/150‑1 (MQ=255)
                                                                                                   tCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGt                                                    <  1:2717090/150‑1 (MQ=255)
                                                                                                          cGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCAtttt                                             <  1:4465732/150‑1 (MQ=255)
                                                                                                             aaCGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACg                                          >  1:977437/1‑150 (MQ=255)
                                                                                                               cGCCAAGACCAAGCAGTAAGAGATTGACCCAACCTGCCAATCCATCAGCCCGATAGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGACGTCATCCTGCAGCTCATCGCTGGTGGCAGAGTGCATTTTACGCa                                        >  2:2887048/1‑150 (MQ=255)
                                                                                                               cGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCa                                        >  1:3973473/1‑150 (MQ=255)
                                                                                                               cGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCa                                        <  1:2505773/150‑1 (MQ=255)
                                                                                                                gCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAg                                       >  2:4480209/1‑150 (MQ=255)
                                                                                                                   aaGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGc                                    <  1:3830245/150‑1 (MQ=255)
                                                                                                                   aaGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGc                                    >  1:2266726/1‑150 (MQ=255)
                                                                                                                       ccAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCtgt                                >  2:1388590/1‑150 (MQ=255)
                                                                                                                        cAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCtgtg                               >  2:206877/1‑150 (MQ=255)
                                                                                                                        cAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCtgtg                               <  1:1599133/150‑1 (MQ=255)
                                                                                                                          aGCAGGAAGTGATTGACCCAACCTGCCTATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGcc                             >  1:1139/1‑150 (MQ=255)
                                                                                                                           gCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCa                            <  1:3252560/150‑1 (MQ=255)
                                                                                                                           gCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCa                            <  1:2587614/150‑1 (MQ=255)
                                                                                                                            cAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAg                           <  1:4103305/150‑1 (MQ=255)
                                                                                                                            cAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAg                           >  1:1111117/1‑150 (MQ=255)
                                                                                                                             aGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGc                          >  2:528314/1‑150 (MQ=255)
                                                                                                                             aGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGc                          >  2:45169/1‑150 (MQ=255)
                                                                                                                                  gTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATg                     >  2:2594433/1‑150 (MQ=255)
                                                                                                                                    gATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGAc                   <  2:1322982/150‑1 (MQ=255)
                                                                                                                                     aTTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGAcc                  <  2:1870131/150‑1 (MQ=255)
                                                                                                                                     aTTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGAcc                  <  2:3094033/150‑1 (MQ=255)
                                                                                                                                     aTTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGAcc                  <  2:3094004/150‑1 (MQ=255)
                                                                                                                                       tGACCCAACCTGCCTATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGa                >  1:3092379/1‑150 (MQ=255)
                                                                                                                                       tGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGa                >  2:1994162/1‑150 (MQ=255)
                                                                                                                                           ccAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGc            <  2:2421359/150‑1 (MQ=255)
                                                                                                                                             aaCCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCa          >  2:1310593/1‑150 (MQ=255)
                                                                                                                                             aaCCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCa          >  2:1130191/1‑150 (MQ=255)
                                                                                                                                              aCCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCAt         >  1:1272735/1‑150 (MQ=255)
                                                                                                                                                 tGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGcc      <  1:2233453/150‑1 (MQ=255)
                                                                                                                                                 tGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCATCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGcc      >  1:2813808/1‑150 (MQ=255)
                                                                                                                                                    cAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCttc   >  1:1322573/1‑150 (MQ=255)
                                                                                                                                                    cAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCttc   >  2:1151211/1‑150 (MQ=255)
                                                                                                                                                     aaTGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCttcg  <  1:3370598/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCG  >  CP009273/3048341‑3048639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑