BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,065,300	C→A	43.4%	D228Y (GAT→TAT)	pgk ←	phosphoglycerate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,065,300	0	C	A	43.4%	317.5 / inf	310	D228Y (GAT→TAT)	pgk	phosphoglycerate kinase
Reads supporting (aligned to +/- strand): ref base C (101/74); new base A (66/69); total (167/143)
Fisher's exact test for biased strand distribution p-value = 1.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 316 total aligned reads displayed.

CACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGTTCTTTCA  >  CP009273/3065151‑3065449

cacaTCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATc                                                                                                                                                       <  2:3544733/150‑1 (MQ=255)

   aTCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTg                                                                                                                                                    >  2:2870885/1‑150 (MQ=255)

     cGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGc                                                                                                                                                  >  1:1423399/1‑150 (MQ=255)

      gTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGcc                                                                                                                                                 >  2:2763528/1‑150 (MQ=255)

       ttAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCt                                                                                                                                                >  2:1834669/1‑150 (MQ=255)

       ttAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCt                                                                                                                                                >  2:2103893/1‑150 (MQ=255)

        tAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCtt                                                                                                                                               >  1:4492039/1‑150 (MQ=255)

          aCAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTtgt                                                                                                                                             <  1:4122970/150‑1 (MQ=255)

           cAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTtgtg                                                                                                                                            >  1:3823493/1‑150 (MQ=255)

                ttCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCcgcg                                                                                                                                       >  1:4310944/1‑150 (MQ=255)

                  ccgggTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTTCGCGAACATCAGACGGAACCGGGATGTTGCAGGTTGTCAGCAGACGTTTATCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGAt                                                                                                                                     <  1:3301268/148‑1 (MQ=255)

                   aGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGGGCCGCGATa                                                                                                                                    <  2:4171988/150‑1 (MQ=255)

                    gggTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATaa                                                                                                                                   >  1:3166621/1‑150 (MQ=255)

                    gggTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATaa                                                                                                                                   <  1:2206216/150‑1 (MQ=255)

                      gTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAg                                                                                                                                 <  2:3864386/150‑1 (MQ=255)

                      gTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAg                                                                                                                                 <  1:2921390/150‑1 (MQ=255)

                       tAGCCGGTGTAGTTTCAGAGAACTCGGTTGCTACGAGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTTCCCACATAGTGGCCTTGTGCCGCGATAAAgg                                                                                                                                <  2:1353587/150‑1 (MQ=255)

                       tAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAgg                                                                                                                                <  2:1265433/150‑1 (MQ=255)

                        aGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGgt                                                                                                                               <  1:2306340/150‑1 (MQ=255)

                            ggTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTa                                                                                                                           <  1:3216269/150‑1 (MQ=255)

                            ggTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGAGAAAGGTGTTa                                                                                                                           >  2:731391/1‑150 (MQ=255)

                             gTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAg                                                                                                                          >  1:2564427/1‑150 (MQ=255)

                             gTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAg                                                                                                                          >  2:4541969/1‑150 (MQ=255)

                              tGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGc                                                                                                                         <  2:3033421/150‑1 (MQ=255)

                              tGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGc                                                                                                                         <  1:2927665/150‑1 (MQ=255)

                               gCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCg                                                                                                                        <  1:15547/150‑1 (MQ=255)

                                  gTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATa                                                                                                                     >  2:3725653/1‑150 (MQ=255)

                                  gTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATa                                                                                                                     >  2:3771366/1‑150 (MQ=255)

                                     tCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATacca                                                                                                                  >  1:3655736/1‑150 (MQ=255)

                                     tCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATacca                                                                                                                  >  1:1012468/1‑150 (MQ=255)

                                     tCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATacca                                                                                                                  >  1:1658718/1‑150 (MQ=255)

                                        gagaACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATaccacca                                                                                                               >  2:2327539/1‑150 (MQ=255)

                                           aaCTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATAccaccacca                                                                                                            >  1:1922011/1‑150 (MQ=255)

                                           aaCTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATAccaccacca                                                                                                            <  2:913534/150‑1 (MQ=255)

                                              tCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACcaaca                                                                                                         >  1:625054/1‑150 (MQ=255)

                                               cGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACcaacaa                                                                                                        >  1:2747481/1‑150 (MQ=255)

                                                ggTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAAt                                                                                                       >  1:3953961/1‑150 (MQ=255)

                                                   tGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAg                                                                                                    <  1:4274684/150‑1 (MQ=255)

                                                    gCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGc                                                                                                   >  2:145774/1‑150 (MQ=255)

                                                      tACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTg                                                                                                 >  1:1135103/1‑150 (MQ=255)

                                                      tACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTg                                                                                                 <  1:1962250/150‑1 (MQ=255)

                                                         gcgAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTc                                                                                              <  1:1485133/150‑1 (MQ=255)

                                                         gcgAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTc                                                                                              <  2:4566830/150‑1 (MQ=255)

                                                              cATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCTGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGa                                                                                         <  1:287097/150‑1 (MQ=255)

                                                              cATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGa                                                                                         >  2:1551123/1‑150 (MQ=255)

                                                              cATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGa                                                                                         >  1:4627947/1‑150 (MQ=255)

                                                               aTCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGAt                                                                                        <  1:345582/150‑1 (MQ=255)

                                                               aTCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGAt                                                                                        >  2:3383488/1‑150 (MQ=255)

                                                               aTCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGAt                                                                                        >  2:2496175/1‑150 (MQ=255)

                                                                tCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGAtt                                                                                       >  1:263762/1‑150 (MQ=255)

                                                                  aGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGAtttt                                                                                     >  2:3396414/1‑150 (MQ=255)

                                                                  aGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGAtttt                                                                                     <  2:4508408/150‑1 (MQ=255)

                                                                   gACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACACGGATTAACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTa                                                                                    >  2:2385168/1‑150 (MQ=255)

                                                                     cGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGa                                                                                  <  2:3274782/150‑1 (MQ=255)

                                                                     cGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGa                                                                                  >  1:2426805/1‑150 (MQ=255)

                                                                      ggAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGAc                                                                                 <  1:1371030/150‑1 (MQ=255)

                                                                      ggAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCCTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGAc                                                                                 <  1:2699269/150‑1 (MQ=255)

                                                                       gAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACa                                                                                >  2:3936200/1‑150 (MQ=255)

                                                                        aaCCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAg                                                                               >  2:2144788/1‑150 (MQ=255)

                                                                         aCCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAgg                                                                              <  1:3569686/150‑1 (MQ=255)

                                                                         aCCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAgg                                                                              <  1:2540130/150‑1 (MQ=255)

                                                                           cGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGa                                                                            <  2:3434325/150‑1 (MQ=255)

                                                                            gggATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAg                                                                           >  1:4214893/1‑150 (MQ=255)

                                                                            gggATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAg                                                                           >  2:2659695/1‑150 (MQ=255)

                                                                                tGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCa                                                                       <  1:1440315/150‑1 (MQ=255)

                                                                                tGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCa                                                                       <  2:2260023/150‑1 (MQ=255)

                                                                                 gTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAg                                                                      >  1:8532/1‑150 (MQ=255)

                                                                                    gCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAAc                                                                   <  1:222032/150‑1 (MQ=255)

                                                                                         tggtCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCa                                                              <  2:4078900/150‑1 (MQ=255)

                                                                                           gtCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGt                                                            <  2:4199497/150‑1 (MQ=255)

                                                                                                cagACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGt                                                       <  1:3374522/150‑1 (MQ=255)

                                                                                                      tttAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATAc                                                 >  1:3519112/1‑150 (MQ=255)

                                                                                                          gCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTa                                             <  1:2360686/150‑1 (MQ=255)

                                                                                                            ttCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATAAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGa                                           >  2:648938/1‑150 (MQ=255)

                                                                                                              cGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAac                                         >  1:3760522/1‑150 (MQ=255)

                                                                                                                   aCCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAAccacca                                    >  2:3664082/1‑150 (MQ=255)

                                                                                                                   aCCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAAccacca                                    >  2:544048/1‑150 (MQ=255)

                                                                                                                     cAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAAc                                  <  1:3650651/150‑1 (MQ=255)

                                                                                                                     cAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAAc                                  >  2:4315411/1‑150 (MQ=255)

                                                                                                                     cAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAAc                                  <  2:130164/150‑1 (MQ=255)

                                                                                                                     cAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAAc                                  >  2:1956549/1‑150 (MQ=255)

                                                                                                                      aGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACg                                 <  2:403982/150‑1 (MQ=255)

                                                                                                                        gTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGAt                               >  2:498194/1‑150 (MQ=255)

                                                                                                                            gCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGcc                           <  2:2021973/150‑1 (MQ=255)

                                                                                                                               tCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGccacc                        <  1:3307443/150‑1 (MQ=255)

                                                                                                                                cGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGccacca                       <  1:4574153/150‑1 (MQ=255)

                                                                                                                                cGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGccacca                       >  2:2927567/1‑150 (MQ=255)

                                                                                                                                  tACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATc                     >  1:3894914/1‑150 (MQ=255)

                                                                                                                                   aCAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCg                    <  2:330041/150‑1 (MQ=255)

                                                                                                                                       ggATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCg                <  2:1319378/150‑1 (MQ=255)

                                                                                                                                        gATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGa               <  1:2472314/150‑1 (MQ=255)

                                                                                                                                         aTTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAg              >  1:964822/1‑150 (MQ=255)

                                                                                                                                            tACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAg           >  2:4231135/1‑150 (MQ=255)

                                                                                                                                              cccACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGt         <  2:1694144/150‑1 (MQ=255)

                                                                                                                                               ccACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGtt        >  1:126056/1‑150 (MQ=255)

                                                                                                                                               ccACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGtt        <  1:4156904/150‑1 (MQ=255)

                                                                                                                                                 acaTCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGTTCt      <  1:1809590/150‑1 (MQ=255)

                                                                                                                                                 acaTAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGTTCt      >  2:855328/1‑150 (MQ=255)

                                                                                                                                                    tCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGTTCTTTc   <  1:2942487/150‑1 (MQ=255)

                                                                                                                                                     cGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCACCAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGTTCTTTCa  <  1:1867248/150‑1 (MQ=255)

CACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGTTCTTTCA  >  CP009273/3065151‑3065449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`