Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,066,958 G→T 5.7% T31T (ACC→ACA)  epd ← D‑erythrose 4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,066,9580GT 5.7% 1081.7 / 25.9 280T31T (ACC→ACA) epdD‑erythrose 4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (136/128);  new base T (9/7);  total (145/135)
Fisher's exact test for biased strand distribution p-value = 8.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 285 total aligned reads displayed.

CGTTCATGCAATACGCGGATGGCGTCATCACCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAAT  >  CP009273/3066809‑3067107
                                                                                                                                                     |                                                                                                                                                     
cGTTCATGCAATACGCGGATGGCGTCATCACCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACg                                                                                                                                                       >  2:1214390/1‑150 (MQ=255)
cGTTCATGCAATACGCGGATGGCGTCATCACCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACg                                                                                                                                                       >  2:3533123/1‑150 (MQ=255)
  ttCATGCAATACGCGGATGGCGTCATCACCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGt                                                                                                                                                     >  2:1638570/1‑150 (MQ=255)
   tCATGCAATACGCGGATGGCGTCATCACCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTa                                                                                                                                                    <  1:3122192/150‑1 (MQ=255)
   tCATGCAATACGCGGATGGCGTCATCACCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTa                                                                                                                                                    >  2:2300633/1‑150 (MQ=255)
      tGCAATACGCGGATGGCGTCATCACCAACAAAAAGTTGATCGCGTTCATGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAAtt                                                                                                                                                 <  1:216716/150‑1 (MQ=255)
         aaTACGCGGATGGCGTCATCACCAACAAAAAGTTGATCGCGTTCCTGTCGTACTGCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTcc                                                                                                                                              <  2:174049/150‑1 (MQ=255)
            aCGCGGATGGCGTCATCACCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGcc                                                                                                                                           <  2:2199830/150‑1 (MQ=255)
               cgGATGGCGTCATCACCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACTGTAATTTCCGCCCgg                                                                                                                                        >  1:1038463/1‑150 (MQ=255)
                ggATGGCGTCATCACCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGc                                                                                                                                       >  1:1154508/1‑150 (MQ=255)
                 gATGGCGTCATCACCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCg                                                                                                                                      <  1:4158249/150‑1 (MQ=255)
                  aTGGCGTCATCACCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGt                                                                                                                                     <  2:621908/150‑1 (MQ=255)
                   tGGCGTCATCACCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTc                                                                                                                                    >  2:1166465/1‑150 (MQ=255)
                       gTCATCACCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGa                                                                                                                                >  1:2970724/1‑150 (MQ=255)
                        tcatcaCCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGAt                                                                                                                               <  2:21353/150‑1 (MQ=255)
                          atcaCCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTc                                                                                                                             >  2:3313361/1‑150 (MQ=255)
                          atcaCCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTc                                                                                                                             >  2:647043/1‑150 (MQ=255)
                                  caaAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAg                                                                                                                     <  2:4250874/150‑1 (MQ=255)
                                   aaaaaGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGc                                                                                                                    >  2:3663443/1‑150 (MQ=255)
                                   aaaaaGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGc                                                                                                                    >  2:3116126/1‑150 (MQ=255)
                                   aaaaaGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGc                                                                                                                    >  2:794628/1‑150 (MQ=255)
                                    caaaGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCa                                                                                                                   <  1:309194/149‑1 (MQ=255)
                                     aaaGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCAc                                                                                                                  <  1:3181937/150‑1 (MQ=255)
                                     aaaGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCAc                                                                                                                  <  2:4250953/150‑1 (MQ=255)
                                      aaGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACTGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACg                                                                                                                 >  2:2532304/1‑150 (MQ=255)
                                        gTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGaa                                                                                                               >  2:2162028/1‑150 (MQ=255)
                                        gTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGaa                                                                                                               >  2:856437/1‑150 (MQ=255)
                                         ttGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACTGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAAc                                                                                                              <  1:1916527/150‑1 (MQ=255)
                                         ttGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAAc                                                                                                              <  1:2243946/150‑1 (MQ=255)
                                             tCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACcaca                                                                                                          >  2:930648/1‑150 (MQ=255)
                                               gcgTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACAtt                                                                                                        <  1:3517536/150‑1 (MQ=255)
                                                 gTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTAc                                                                                                      >  2:3777938/1‑150 (MQ=255)
                                                 gTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTAc                                                                                                      >  1:2649732/1‑150 (MQ=255)
                                                    ccTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGcc                                                                                                   >  1:3956540/1‑150 (MQ=255)
                                                          gTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGc                                                                                             >  1:384628/1‑150 (MQ=255)
                                                          gTACTTCCCAAGCAAAACGGCCATGGCAGGTGTCAAATTTCAACAAATGCGCCATGCACGCATCAACCGCCAGTTCGTTGATTGCCACCACGGTCATGGCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGc                                                                                             >  2:376157/1‑150 (MQ=255)
                                                            acgtCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGa                                                                                           <  2:1045797/147‑1 (MQ=255)
                                                                    tGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGcc                                                                                   <  2:1113525/150‑1 (MQ=255)
                                                                       aaaaCGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCAtt                                                                                >  1:2739399/1‑150 (MQ=255)
                                                                       aaaaCGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCAtt                                                                                >  2:466566/1‑150 (MQ=255)
                                                                        aaaCGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACTGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCAttt                                                                               <  2:565946/150‑1 (MQ=255)
                                                                        aaaCGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCAttt                                                                               >  2:2367386/1‑150 (MQ=255)
                                                                        aaaCGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCAttt                                                                               <  2:347824/150‑1 (MQ=255)
                                                                        aaaCGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCAttt                                                                               >  2:3813566/1‑150 (MQ=255)
                                                                         aaCGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTa                                                                              >  1:3829684/1‑150 (MQ=255)
                                                                          aCGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTAt                                                                             <  2:3684518/150‑1 (MQ=255)
                                                                          aCGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTAt                                                                             <  1:3544653/150‑1 (MQ=255)
                                                                            ggCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCg                                                                           >  1:1240225/1‑150 (MQ=255)
                                                                             gCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGc                                                                          <  2:3773552/150‑1 (MQ=255)
                                                                                aTGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTAc                                                                       >  2:1353560/1‑150 (MQ=255)
                                                                                aTGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTAc                                                                       >  2:306042/1‑150 (MQ=255)
                                                                                     tGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTa                                                                  >  2:4474551/1‑150 (MQ=255)
                                                                                      ggTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCATCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTAc                                                                 <  2:3080609/150‑1 (MQ=255)
                                                                                          tCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACTGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTc                                                             >  1:1078259/1‑150 (MQ=255)
                                                                                           cATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCa                                                            <  2:379344/150‑1 (MQ=255)
                                                                                           cATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCa                                                            >  1:1952568/1‑150 (MQ=255)
                                                                                            atatTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCAt                                                           >  2:1492538/1‑150 (MQ=255)
                                                                                            atatTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATAACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCAt                                                           >  2:3461101/1‑150 (MQ=255)
                                                                                              atTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAg                                                         >  2:3843782/1‑150 (MQ=255)
                                                                                                ttCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGAt                                                       >  2:322271/1‑150 (MQ=255)
                                                                                                ttCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGAt                                                       <  2:2474035/150‑1 (MQ=255)
                                                                                                ttCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGAt                                                       <  1:240061/150‑1 (MQ=255)
                                                                                                ttCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGAt                                                       <  1:680255/150‑1 (MQ=255)
                                                                                                ttCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGATACGCGTACGGGCATAGAt                                                       >  1:24892/1‑150 (MQ=255)
                                                                                                  caacaaATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGAtct                                                     <  2:2092978/150‑1 (MQ=255)
                                                                                                   aacaaATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGAtctc                                                    >  2:4511068/1‑150 (MQ=255)
                                                                                                     caaATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACTGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCt                                                  <  2:2997277/150‑1 (MQ=255)
                                                                                                     caaATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCt                                                  <  2:1935475/150‑1 (MQ=255)
                                                                                                        aTGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCa                                               >  1:18197/1‑150 (MQ=255)
                                                                                                          gcgcCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAg                                             <  2:823841/150‑1 (MQ=255)
                                                                                                            gcCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGt                                           >  1:2945083/1‑150 (MQ=255)
                                                                                                            gcCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGt                                           <  2:1916997/150‑1 (MQ=255)
                                                                                                             ccATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGtt                                          >  1:4439624/1‑150 (MQ=255)
                                                                                                              cATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGttt                                         >  1:3646576/1‑150 (MQ=255)
                                                                                                                tGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCCTTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTc                                       >  1:992617/1‑150 (MQ=255)
                                                                                                                tGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTc                                       >  1:3310312/1‑150 (MQ=255)
                                                                                                                tGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTc                                       <  2:185347/150‑1 (MQ=255)
                                                                                                                tGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTc                                       <  2:184750/150‑1 (MQ=255)
                                                                                                                         caTCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCaa                              <  2:3956540/150‑1 (MQ=255)
                                                                                                                         caTCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCaa                              <  1:270393/150‑1 (MQ=255)
                                                                                                                          aTCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCaaa                             >  1:1663337/1‑150 (MQ=255)
                                                                                                                          aTCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCaaa                             >  1:3902588/1‑150 (MQ=255)
                                                                                                                            ccGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAAtt                           <  1:4591039/150‑1 (MQ=255)
                                                                                                                             cGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAAttt                          <  2:2828957/150‑1 (MQ=255)
                                                                                                                              gCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTg                         >  2:3699391/1‑150 (MQ=255)
                                                                                                                              gCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTg                         <  1:756294/150‑1 (MQ=255)
                                                                                                                                cAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGcc                       >  1:54642/1‑150 (MQ=255)
                                                                                                                                cAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGcc                       <  1:3548250/150‑1 (MQ=255)
                                                                                                                                 aGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCca                      <  2:3282257/150‑1 (MQ=255)
                                                                                                                                 aGTTCGTTGATTGCCACCACGGTAATTTCCGCCAGGCGTCCGTATTCATACAAAGCACGAACCTCAATACTCCAGATGCGACCGTAGCCATTTATCGCTACGCGTAAGGTCATAGATATCCTGCAAGGTTTTCCCTGAGCAAATTTGCca                      <  2:704463/150‑1 (MQ=255)
                                                                                                                                    tCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCcagac                   >  1:600090/1‑150 (MQ=255)
                                                                                                                                    tCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCcagac                   <  2:1267465/150‑1 (MQ=255)
                                                                                                                                     cGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCcagaca                  <  1:4071564/150‑1 (MQ=255)
                                                                                                                                     cGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCcagaca                  >  1:886112/1‑150 (MQ=255)
                                                                                                                                        tGATTGCCACCACTGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACagag               >  1:2110204/1‑150 (MQ=255)
                                                                                                                                        tGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACagag               <  1:3447553/150‑1 (MQ=255)
                                                                                                                                          aTTGCCACCACTGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTa             >  2:4115482/1‑150 (MQ=255)
                                                                                                                                           ttGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTaa            <  1:2295338/150‑1 (MQ=255)
                                                                                                                                                     ggTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAAt  >  2:2432003/1‑150 (MQ=255)
                                                                                                                                                     ggTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAAt  <  1:1460813/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGTTCATGCAATACGCGGATGGCGTCATCACCAACAAAAAGTTGATCGCGTTCCTGTCGTACTTCCCATGCAAAACGGCCATGGCTGGTGTCATATTTCAACAAATGCGCCATGCCCGCAGCATCCGCCAGTTCGTTGATTGCCACCACGGTAATTTCCGCCCGGCGTCCGGATTCATACAAAGCACGAACCACATTACGCCCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAAT  >  CP009273/3066809‑3067107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑