BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,085,649	C→A	7.7%	T138K (ACG→AAG)	gshB →	glutathione synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,085,649	0	C	A	7.7%	1082.5 / 41.6	285	T138K (ACG→AAG)	gshB	glutathione synthetase
Reads supporting (aligned to +/- strand): ref base C (130/133); new base A (15/7); total (145/140)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 291 total aligned reads displayed.

CCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAACCCTGA  >  CP009273/3085500‑3085798

cccGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAc                                                                                                                                                       <  2:3320825/150‑1 (MQ=255)

cccGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAc                                                                                                                                                       <  2:1617891/150‑1 (MQ=255)

  cgccgTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGc                                                                                                                                                     >  2:2462165/1‑150 (MQ=255)

       tttGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGtt                                                                                                                                                >  1:3080950/1‑150 (MQ=255)

        ttGATCCCGAGTTTATCTACGCGACCTATATTCTGGACCGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCTGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTa                                                                                                                                               <  1:603805/150‑1 (MQ=255)

        ttGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTa                                                                                                                                               <  2:4468766/150‑1 (MQ=255)

         tGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTAc                                                                                                                                              >  2:1855008/1‑150 (MQ=255)

         tGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTAc                                                                                                                                              <  1:415876/150‑1 (MQ=255)

           aTACCGAGTTTATCTACGCGACCTATATTCTGTAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCATAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCAGTTACTCTGACTTAACGACAGAAACGCTGGTTAcgc                                                                                                                                            >  1:619463/1‑150 (MQ=255)

            tACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTAcgcg                                                                                                                                           >  2:4047953/1‑150 (MQ=255)

              ccGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCa                                                                                                                                         <  1:3977179/150‑1 (MQ=255)

               cGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCaa                                                                                                                                        >  1:1024255/1‑150 (MQ=255)

                gAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAAt                                                                                                                                       >  1:2139625/1‑150 (MQ=255)

                gAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAAt                                                                                                                                       >  1:2401034/1‑150 (MQ=255)

                  gTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATaa                                                                                                                                     >  2:636796/1‑150 (MQ=255)

                  gTTTATCTACGCGACATATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCTCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATaa                                                                                                                                     >  1:939990/1‑150 (MQ=255)

                      aTCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAgcg                                                                                                                                 >  2:1142770/1‑150 (MQ=255)

                         tACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAg                                                                                                                              <  2:4190619/150‑1 (MQ=255)

                         tACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAg                                                                                                                              >  1:3447393/1‑150 (MQ=255)

                         tACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAg                                                                                                                              <  2:3437665/150‑1 (MQ=255)

                                    tatTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGtt                                                                                                                   <  1:1065499/150‑1 (MQ=255)

                                         tGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTggg                                                                                                              <  2:1246819/150‑1 (MQ=255)

                                         tGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTggg                                                                                                              <  1:1365396/150‑1 (MQ=255)

                                          ggAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGga                                                                                                             >  1:4496949/1‑150 (MQ=255)

                                           gAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGgag                                                                                                            >  2:3926234/1‑150 (MQ=255)

                                           gAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGgag                                                                                                            >  1:2980340/1‑150 (MQ=255)

                                             aCGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGaa                                                                                                          >  2:2843705/1‑150 (MQ=255)

                                              cGTGCCGAAGAGAACGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGaaa                                                                                                         <  2:43809/150‑1 (MQ=255)

                                                tGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGACACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAaca                                                                                                       <  1:3902679/150‑1 (MQ=255)

                                                  ccGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACCGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAAcaca                                                                                                     <  2:3339555/150‑1 (MQ=255)

                                                    gAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGc                                                                                                   <  1:376713/150‑1 (MQ=255)

                                                      agagAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGa                                                                                                 <  1:4127467/150‑1 (MQ=255)

                                                         gaAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGAcat                                                                                              <  2:1324952/150‑1 (MQ=255)

                                                           aaGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGAcatca                                                                                            <  2:1487920/150‑1 (MQ=255)

                                                           aaGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGAcatca                                                                                            <  2:3784138/150‑1 (MQ=255)

                                                            aGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGAcatcat                                                                                           >  1:3268027/1‑150 (MQ=255)

                                                              ggACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTc                                                                                         <  2:3940106/150‑1 (MQ=255)

                                                              ggACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTc                                                                                         <  2:2876477/150‑1 (MQ=255)

                                                               gACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCt                                                                                        >  1:2535849/1‑150 (MQ=255)

                                                                  gCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTaa                                                                                     <  2:3751865/150‑1 (MQ=255)

                                                                   cTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACTGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAg                                                                                    >  2:661920/1‑150 (MQ=255)

                                                                    tGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGc                                                                                   >  2:422634/1‑150 (MQ=255)

                                                                      aTCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCg                                                                                 <  1:3367028/150‑1 (MQ=255)

                                                                       tCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGc                                                                                >  2:2108569/1‑150 (MQ=255)

                                                                         gTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTg                                                                              <  2:1395738/150‑1 (MQ=255)

                                                                              cAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACgg                                                                         >  2:1777295/1‑150 (MQ=255)

                                                                               aaGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGt                                                                        >  2:2707946/1‑150 (MQ=255)

                                                                               aaGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGt                                                                        >  1:3147486/1‑150 (MQ=255)

                                                                                aGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTa                                                                       <  1:1999809/150‑1 (MQ=255)

                                                                                aGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTa                                                                       >  2:4007124/1‑150 (MQ=255)

                                                                                  ccGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATg                                                                     <  1:4242195/150‑1 (MQ=255)

                                                                                   cGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATgg                                                                    >  1:740977/1‑150 (MQ=255)

                                                                                      agagCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGggcg                                                                 <  2:156348/150‑1 (MQ=255)

                                                                                       gagCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGggcgg                                                                >  1:3037170/1‑150 (MQ=255)

                                                                                       gagCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGggcgg                                                                <  2:2593809/150‑1 (MQ=255)

                                                                                       gagCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGggcgg                                                                >  1:2607941/1‑150 (MQ=255)

                                                                                       gagCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGggcgg                                                                >  1:3555358/1‑150 (MQ=255)

                                                                                       gagCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGggcgg                                                                >  2:4066822/1‑150 (MQ=255)

                                                                                         gCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGgcggcg                                                              <  1:4436092/150‑1 (MQ=255)

                                                                                          ccTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGgcgc                                                             <  1:1571495/150‑1 (MQ=255)

                                                                                            tGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGt                                                           >  1:1706372/1‑150 (MQ=255)

                                                                                              cgcgACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCg                                                         <  2:2615131/150‑1 (MQ=255)

                                                                                              cgcgACTGTAACGAGAAACTGTTGACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCg                                                         <  1:5575/150‑1 (MQ=255)

                                                                                                cgACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGAt                                                       <  1:2732050/150‑1 (MQ=255)

                                                                                                 gACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCCATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGAtt                                                      <  1:1215618/150‑1 (MQ=255)

                                                                                                 gACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGAtt                                                      <  2:311475/150‑1 (MQ=255)

                                                                                                 gACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGAtt                                                      >  2:1001890/1‑150 (MQ=255)

                                                                                                 gACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGAtt                                                      >  1:435163/1‑150 (MQ=255)

                                                                                                  aCTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGAttt                                                     >  2:3603146/1‑150 (MQ=255)

                                                                                                    tGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTc                                                   >  2:4606899/1‑150 (MQ=255)

                                                                                                     gTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTcc                                                  <  1:1482857/150‑1 (MQ=255)

                                                                                                      tAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCcg                                                 <  1:1334964/150‑1 (MQ=255)

                                                                                                      tAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCcg                                                 >  2:4633986/1‑150 (MQ=255)

                                                                                                        aCGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCcgcg                                               <  1:4063152/150‑1 (MQ=255)

                                                                                                           agaAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGa                                            >  2:4143064/1‑150 (MQ=255)

                                                                                                              aaCTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAaag                                         <  1:2255105/150‑1 (MQ=255)

                                                                                                                  gTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCGGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAgg                                     >  2:71146/1‑150 (MQ=255)

                                                                                                                    ttACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCg                                   >  2:2306708/1‑150 (MQ=255)

                                                                                                                     tACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGa                                  >  1:1071662/1‑150 (MQ=255)

                                                                                                                          ccTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCaa                             <  2:247084/150‑1 (MQ=255)

                                                                                                                           cTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCaaa                            >  1:1921853/1‑150 (MQ=255)

                                                                                                                            tGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAAc                           >  2:2200279/1‑150 (MQ=255)

                                                                                                                            tGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAAc                           >  2:235553/1‑150 (MQ=255)

                                                                                                                             ggTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAAcc                          >  1:1892369/1‑150 (MQ=255)

                                                                                                                             ggTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAAcc                          >  1:2558332/1‑150 (MQ=255)

                                                                                                                              gTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCt                         >  2:3204096/1‑150 (MQ=255)

                                                                                                                                tctcTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCg                       <  1:2793848/150‑1 (MQ=255)

                                                                                                                                 ctctGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCgg                      <  1:2952869/150‑1 (MQ=255)

                                                                                                                                    tGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGt                   >  2:608590/1‑150 (MQ=255)

                                                                                                                                     gACTTAACGCCAGAAACGCGGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTg                  >  2:777123/1‑150 (MQ=255)

                                                                                                                                      aCTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGa                 >  1:4611610/1‑150 (MQ=255)

                                                                                                                                        ttAACGCCAGAAACGCTGGTTACGCTCAATCAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGAtt               <  1:838778/150‑1 (MQ=255)

                                                                                                                                         tAACGCCTGTAACGCTGGTTACGCGCAATTTAGCGGTGCTAAATGCGTTCTGGGAGAAACACAGCGAGATCATTCTTAAGCCGCTGGACGGTTTGGGCGGCGGGTCGTTTTTCCGCGGGTAAGATGGCGTTCCATAGCTCTGCGTGATTg              <  2:619463/150‑1 (MQ=255)

                                                                                                                                         tAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTg              >  2:3247136/1‑150 (MQ=255)

                                                                                                                                          aaCGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGc             >  1:2326484/1‑150 (MQ=255)

                                                                                                                                          aaCGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGc             >  1:3489123/1‑150 (MQ=255)

                                                                                                                                                aGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAAc       >  1:3690757/1‑150 (MQ=255)

                                                                                                                                                aGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAAc       <  2:1267297/150‑1 (MQ=255)

                                                                                                                                                   aaCGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAACCct    <  1:1977330/150‑1 (MQ=255)

                                                                                                                                                     cGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAACCctga  >  2:1047979/1‑150 (MQ=255)

CCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAACCCTGA  >  CP009273/3085500‑3085798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`