Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,092,182 C→A 15.2% C248F (TGT→TTT)  yggM ← DUF1202 family putative secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,092,1820CA15.2% 913.4 / 93.2 284C248F (TGT→TTT) yggMDUF1202 family putative secreted protein
Reads supporting (aligned to +/- strand):  ref base C (138/102);  new base A (24/20);  total (162/122)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

AAACTTTCATCGTTAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCATTTGC  >  CP009273/3092033‑3092331
                                                                                                                                                     |                                                                                                                                                     
  aCTTTCATCGTTAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAacac                                                                                                                                                     >  1:4378240/1‑150 (MQ=255)
      tCATCGTTAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCtt                                                                                                                                                 <  1:1325126/150‑1 (MQ=255)
        aTCGTTAACGGATATTGCCCTTTTTCGAGCGTTACCAAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGAAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCa                                                                                                                                               >  2:2481903/1‑150 (MQ=255)
          cGTTAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCAct                                                                                                                                             >  1:1095295/1‑150 (MQ=255)
             tAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTg                                                                                                                                          >  2:320941/1‑150 (MQ=255)
             tAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTg                                                                                                                                          >  2:3422535/1‑150 (MQ=255)
              aaCGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGt                                                                                                                                         >  2:1317598/1‑150 (MQ=255)
                cGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGttt                                                                                                                                       <  1:3221840/150‑1 (MQ=255)
                 ggATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTa                                                                                                                                      >  1:4255821/1‑150 (MQ=255)
                  gATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTAt                                                                                                                                     <  2:4327914/150‑1 (MQ=255)
                    tatTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGa                                                                                                                                   >  2:3636560/1‑150 (MQ=255)
                      ttGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACa                                                                                                                                 >  1:192844/1‑150 (MQ=255)
                        gCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATg                                                                                                                               >  1:2405307/1‑150 (MQ=255)
                        gCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATg                                                                                                                               >  1:2405474/1‑150 (MQ=255)
                         cccTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGc                                                                                                                              >  1:1700474/1‑150 (MQ=255)
                         cccTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGc                                                                                                                              >  1:1497364/1‑150 (MQ=255)
                          ccTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGcc                                                                                                                             <  1:342737/150‑1 (MQ=255)
                           ccttttCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCg                                                                                                                            <  2:3134157/148‑1 (MQ=255)
                               ttCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAAt                                                                                                                        >  1:1619800/1‑150 (MQ=255)
                                tCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATc                                                                                                                       <  2:2694540/150‑1 (MQ=255)
                                 cGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCg                                                                                                                      >  1:2859070/1‑150 (MQ=255)
                                  gAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGc                                                                                                                     >  1:4114942/1‑150 (MQ=255)
                                        tACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTg                                                                                                               <  1:2996704/150‑1 (MQ=255)
                                               tCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAAcc                                                                                                        <  2:3162885/150‑1 (MQ=255)
                                               tCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGACACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAAcc                                                                                                        >  1:2095965/1‑150 (MQ=255)
                                                cATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACct                                                                                                       <  1:3637766/150‑1 (MQ=255)
                                                 aTCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACctc                                                                                                      <  2:1296596/150‑1 (MQ=255)
                                                  tCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACctct                                                                                                     <  2:4467713/150‑1 (MQ=255)
                                                    cTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCttg                                                                                                   >  2:2172744/1‑149 (MQ=255)
                                                     tGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTg                                                                                                  <  2:2884698/150‑1 (MQ=255)
                                                     tGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTg                                                                                                  <  2:2270523/150‑1 (MQ=255)
                                                      gCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGt                                                                                                 >  2:3451783/1‑150 (MQ=255)
                                                       cAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTc                                                                                                <  1:4148772/150‑1 (MQ=255)
                                                       cAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTc                                                                                                >  1:1172134/1‑150 (MQ=255)
                                                       cAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTc                                                                                                >  2:4057385/1‑150 (MQ=255)
                                                        aaTTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCa                                                                                               >  1:604829/1‑150 (MQ=255)
                                                        aaTTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCa                                                                                               >  2:4540370/1‑150 (MQ=255)
                                                         aTTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCat                                                                                              >  2:3550731/1‑150 (MQ=255)
                                                         aTTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCat                                                                                              >  1:648227/1‑150 (MQ=255)
                                                          tttACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCata                                                                                             >  1:275526/1‑150 (MQ=255)
                                                            tACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATAtt                                                                                           >  1:2167290/1‑150 (MQ=255)
                                                            tACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATAtt                                                                                           <  1:4048236/150‑1 (MQ=255)
                                                             aCGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATAttt                                                                                          <  2:914363/150‑1 (MQ=255)
                                                                 cTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAAcc                                                                                      >  2:4442074/1‑150 (MQ=255)
                                                                 cTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAAcc                                                                                      <  1:2534694/150‑1 (MQ=255)
                                                                   aaGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACcgc                                                                                    >  1:346145/1‑150 (MQ=255)
                                                                   aaGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACcgc                                                                                    >  1:519594/1‑150 (MQ=255)
                                                                    aGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACcgcg                                                                                   <  2:2645138/150‑1 (MQ=255)
                                                                    aGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACcgcg                                                                                   <  1:4478941/150‑1 (MQ=255)
                                                                    aGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACcgcg                                                                                   <  2:4348408/150‑1 (MQ=255)
                                                                       ttttGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAAc                                                                                <  2:2513400/150‑1 (MQ=255)
                                                                          tgtgATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGcc                                                                             <  2:3314588/150‑1 (MQ=255)
                                                                                 gCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTAt                                                                      >  2:1994759/1‑150 (MQ=255)
                                                                                   aGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCg                                                                    >  1:102723/1‑150 (MQ=255)
                                                                                       aaGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCAGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGAAATGCCGCAATCGCCGGTTGATAAACCTCTTTGTAATATTTAACCGCGAACGCCTCGCTATCGTTCt                                                                >  2:1171039/1‑150 (MQ=255)
                                                                                        aGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTg                                                               >  1:2115595/1‑150 (MQ=255)
                                                                                           cTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGttt                                                            >  1:1048269/1‑150 (MQ=255)
                                                                                             gTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTttat                                                          >  1:4608754/1‑150 (MQ=255)
                                                                                              tCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTttatt                                                         >  1:2909434/1‑150 (MQ=255)
                                                                                               cGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTttatta                                                        >  1:3827444/1‑150 (MQ=255)
                                                                                                   gttgttCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACt                                                    <  1:2341458/150‑1 (MQ=255)
                                                                                                     tgttCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACtca                                                  >  2:2293175/1‑150 (MQ=255)
                                                                                                     tgttCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACtca                                                  >  2:523376/1‑150 (MQ=255)
                                                                                                     tgttCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACtca                                                  >  1:89569/1‑150 (MQ=255)
                                                                                                         cGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCAc                                              <  1:4517197/150‑1 (MQ=255)
                                                                                                             gATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTg                                          <  2:1783216/150‑1 (MQ=255)
                                                                                                              atatCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGt                                         >  2:1630517/1‑150 (MQ=255)
                                                                                                               tatCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGtt                                        <  2:4169719/150‑1 (MQ=255)
                                                                                                                atCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTg                                       >  2:1675199/1‑150 (MQ=255)
                                                                                                                atCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTg                                       <  1:2571747/150‑1 (MQ=255)
                                                                                                                 tCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTgg                                      <  1:389014/150‑1 (MQ=255)
                                                                                                                   gAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTg                                    >  2:966379/1‑150 (MQ=255)
                                                                                                                   gAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTg                                    >  1:3547766/1‑150 (MQ=255)
                                                                                                                      aTCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAAt                                 <  2:1021075/150‑1 (MQ=255)
                                                                                                                        ctcGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAAttt                               >  2:2159689/1‑150 (MQ=255)
                                                                                                                           gTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAAtttttt                            >  2:2616639/1‑150 (MQ=255)
                                                                                                                           gTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCATTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGACtttttt                            >  2:1519726/1‑150 (MQ=255)
                                                                                                                            tttCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAAttttttt                           >  2:3186633/1‑150 (MQ=255)
                                                                                                                            tttCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAAttttttt                           <  2:3934715/150‑1 (MQ=255)
                                                                                                                            tttCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAAttttttt                           <  2:375035/150‑1 (MQ=255)
                                                                                                                                  ctgAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGc                     <  1:938875/150‑1 (MQ=255)
                                                                                                                                  ctgAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAAACGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGTTGAATTTTTTTAAAGGc                     >  1:4634274/1‑150 (MQ=255)
                                                                                                                                   tgAATCGGCACTTCATAACACTCTTCAATCTGTTTTTGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCg                    <  1:3779787/150‑1 (MQ=255)
                                                                                                                                    gAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGt                   >  1:3347773/1‑150 (MQ=255)
                                                                                                                                     aaTCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTc                  >  2:3020632/1‑150 (MQ=255)
                                                                                                                                       tCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCtt                <  1:4200368/150‑1 (MQ=255)
                                                                                                                                        cGGCCCTTCATAACACTCTTCACTCTTTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTCCCCGCGAAAGCCTCTCTATAGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTc               <  1:4563073/150‑1 (MQ=255)
                                                                                                                                           cACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTcgcg            >  2:138973/1‑150 (MQ=255)
                                                                                                                                              ttCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGt         >  2:3447110/1‑150 (MQ=255)
                                                                                                                                                cATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCa       <  1:1897293/150‑1 (MQ=255)
                                                                                                                                                 aTAAAACTCTACACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTTTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTTAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCAt      <  1:2257280/150‑1 (MQ=255)
                                                                                                                                                  tAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCAtt     <  2:4287621/150‑1 (MQ=255)
                                                                                                                                                  tAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCAtt     >  2:1449126/1‑150 (MQ=255)
                                                                                                                                                  tAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCAtt     <  2:3201588/150‑1 (MQ=255)
                                                                                                                                                   aaCACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCAttt    <  2:1357726/150‑1 (MQ=255)
                                                                                                                                                   aaCACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCAttt    <  1:2106877/150‑1 (MQ=255)
                                                                                                                                                   aaCACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTGTTAAAGGCGTCTTCGCGGGTCAttt    >  2:2606794/1‑150 (MQ=255)
                                                                                                                                                    acacTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCATTTg   <  1:2005210/150‑1 (MQ=255)
                                                                                                                                                     cacTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCATTTGc  >  1:2831391/1‑150 (MQ=255)
                                                                                                                                                     cacTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCATTTGc  <  2:2732649/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAACTTTCATCGTTAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCATTTGC  >  CP009273/3092033‑3092331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑