Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,096,445 A→T 13.9% Q25L (CAA→CTA)  mutY → adenine DNA glycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,096,4450AT13.9% 776.8 / 99.0 265Q25L (CAA→CTA) mutYadenine DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base A (108/120);  new base T (16/21);  total (124/141)
Fisher's exact test for biased strand distribution p-value = 7.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

CTGCGGAAAGTTCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCC  >  CP009273/3096296‑3096594
                                                                                                                                                     |                                                                                                                                                     
     gAAAGTTCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTg                                                                                                                                                  <  1:2771036/150‑1 (MQ=255)
     gAAAGTTCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTg                                                                                                                                                  <  1:2588141/150‑1 (MQ=255)
     gAAAGTTCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTg                                                                                                                                                  <  1:1558601/150‑1 (MQ=255)
      aaaGTTCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGACAAACTCTGCCCTGGCAAATTGa                                                                                                                                                 >  2:3922742/1‑150 (MQ=255)
       aaGTTCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGAc                                                                                                                                                <  1:3762732/150‑1 (MQ=255)
        aGTTCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACa                                                                                                                                               <  2:4478044/150‑1 (MQ=255)
          ttCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAg                                                                                                                                             <  2:2921310/150‑1 (MQ=255)
            ccGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGAc                                                                                                                                           >  2:1054691/1‑150 (MQ=255)
             cGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACg                                                                                                                                          >  2:3943032/1‑150 (MQ=255)
             cGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACg                                                                                                                                          <  1:1420098/150‑1 (MQ=255)
                 ttACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCt                                                                                                                                      <  1:1106515/150‑1 (MQ=255)
                 ttACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCt                                                                                                                                      <  2:1790486/150‑1 (MQ=255)
                  tACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTa                                                                                                                                     >  1:3814968/1‑150 (MQ=255)
                   acacCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTAc                                                                                                                                    >  1:3430054/1‑150 (MQ=255)
                   acacCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTAc                                                                                                                                    <  2:860103/150‑1 (MQ=255)
                     acCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACaa                                                                                                                                  >  1:3692115/1‑150 (MQ=255)
                       ccTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAg                                                                                                                                <  2:3380723/150‑1 (MQ=255)
                          gCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGTCTAATTGACAAGACGCCCTACAAAGTAt                                                                                                                             <  2:3784522/150‑1 (MQ=255)
                            cGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATgg                                                                                                                           <  2:243549/150‑1 (MQ=255)
                             gTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGc                                                                                                                          >  2:2592598/1‑150 (MQ=255)
                             gTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGc                                                                                                                          >  2:2117465/1‑150 (MQ=255)
                             gTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGc                                                                                                                          <  1:1378390/150‑1 (MQ=255)
                              tCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGct                                                                                                                         <  2:503600/150‑1 (MQ=255)
                                 cTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCtctc                                                                                                                      <  2:596668/150‑1 (MQ=255)
                                   gtgCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAg                                                                                                                    >  2:924052/1‑150 (MQ=255)
                                    tgctgcAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGa                                                                                                                   >  1:4069697/1‑150 (MQ=255)
                                           aTCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATg                                                                                                            <  1:947806/150‑1 (MQ=255)
                                            tCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGt                                                                                                           >  2:796294/1‑150 (MQ=255)
                                            tCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGt                                                                                                           <  1:2403948/150‑1 (MQ=255)
                                              ttGCCCCCTACAACAGTGATTTCGGTTACCATGGTAGCGTCGATTTTTTCAGCCCAGGTTCTGGAGTGGTACGATAAATACGGGCGAAATACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTATCAGAAGTGATGTTg                                                                                                         <  1:3224334/150‑1 (MQ=255)
                                               tGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGc                                                                                                        >  2:3605654/1‑150 (MQ=255)
                                                 cccccAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGcaa                                                                                                      >  2:1717626/1‑150 (MQ=255)
                                                   cccAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGcaaca                                                                                                    >  2:4051731/1‑150 (MQ=255)
                                                       acaactGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAATAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCATCAAACt                                                                                                <  1:2195332/150‑1 (MQ=255)
                                                       acaacaGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACt                                                                                                <  1:3870324/150‑1 (MQ=255)
                                                            aGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGt                                                                                           >  2:2717939/1‑150 (MQ=255)
                                                                aaTTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCg                                                                                       <  1:267803/150‑1 (MQ=255)
                                                                 aTTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCACCAAACTCAGGTTGCGa                                                                                      >  2:2559755/1‑150 (MQ=255)
                                                                  ttCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGAc                                                                                     >  1:1940918/1‑150 (MQ=255)
                                                                   tCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGAcc                                                                                    <  2:1131/150‑1 (MQ=255)
                                                                   tCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGAcc                                                                                    <  1:499578/150‑1 (MQ=255)
                                                                      gTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGtt                                                                                 >  1:847169/1‑150 (MQ=255)
                                                                      gTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGtt                                                                                 <  2:556678/150‑1 (MQ=255)
                                                                       tGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTa                                                                                <  2:137089/150‑1 (MQ=255)
                                                                       tGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTa                                                                                >  1:630321/1‑150 (MQ=255)
                                                                           cATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATccc                                                                            <  1:2738299/150‑1 (MQ=255)
                                                                             tGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCt                                                                          <  1:3957107/150‑1 (MQ=255)
                                                                              gCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTa                                                                         >  1:2920789/1‑150 (MQ=255)
                                                                                aaGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTAtt                                                                       >  1:4117536/1‑150 (MQ=255)
                                                                                 aGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTAttt                                                                      <  2:2849962/150‑1 (MQ=255)
                                                                                   cGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTg                                                                    <  2:1845011/150‑1 (MQ=255)
                                                                                        cAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGc                                                               >  2:3521017/1‑150 (MQ=255)
                                                                                         aaTTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCt                                                              >  2:2539500/1‑150 (MQ=255)
                                                                                         aaTTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCt                                                              <  1:3546092/150‑1 (MQ=255)
                                                                                            tttCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTa                                                           >  1:1014948/1‑150 (MQ=255)
                                                                                              tCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATg                                                         <  1:180392/150‑1 (MQ=255)
                                                                                              tCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATg                                                         <  1:1275848/150‑1 (MQ=255)
                                                                                               cAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATgg                                                        <  2:1153093/150‑1 (MQ=255)
                                                                                               cAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATgg                                                        <  2:4447718/150‑1 (MQ=255)
                                                                                                aGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGgc                                                       <  1:1331541/150‑1 (MQ=255)
                                                                                                    cAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGgcgcgc                                                   >  1:3585156/1‑150 (MQ=255)
                                                                                                     aGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCt                                                  <  1:208856/150‑1 (MQ=255)
                                                                                                       gTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTc                                                <  1:4232268/150‑1 (MQ=255)
                                                                                                         tCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTccc                                              >  2:2228658/1‑150 (MQ=255)
                                                                                                           tGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGa                                            >  1:3354193/1‑150 (MQ=255)
                                                                                                            ggACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGAc                                           <  2:431319/150‑1 (MQ=255)
                                                                                                             gACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACg                                          <  1:4355086/150‑1 (MQ=255)
                                                                                                             gACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACg                                          <  1:3083880/150‑1 (MQ=255)
                                                                                                              aCTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACgg                                         >  2:3993233/1‑150 (MQ=255)
                                                                                                               cTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGt                                        <  2:2162856/150‑1 (MQ=255)
                                                                                                                 ggTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGa                                      >  1:607910/1‑150 (MQ=255)
                                                                                                                   tACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGAcc                                    <  1:756341/150‑1 (MQ=255)
                                                                                                                   tACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGAcc                                    <  2:1351354/150‑1 (MQ=255)
                                                                                                                    aCGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCGTTATGGCGCGCTTCCCGACGGTGACCg                                   >  2:2638702/1‑150 (MQ=255)
                                                                                                                    aCGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCg                                   <  1:3630778/150‑1 (MQ=255)
                                                                                                                     cGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTGTATGGCGCGCTTCCCGACGGTGACCGa                                  >  2:2238999/1‑150 (MQ=255)
                                                                                                                       aTAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGAtc                                >  1:3393885/1‑150 (MQ=255)
                                                                                                                        tAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGAtct                               <  1:2318184/150‑1 (MQ=255)
                                                                                                                         aaaTACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGAtctc                              >  1:4321855/1‑150 (MQ=255)
                                                                                                                         aaaTACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGAtctc                              <  2:1176235/150‑1 (MQ=255)
                                                                                                                            tACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGcc                           >  1:3174070/1‑150 (MQ=255)
                                                                                                                             aCGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCa                          >  1:1224272/1‑150 (MQ=255)
                                                                                                                                ggCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATg                       <  2:1274180/150‑1 (MQ=255)
                                                                                                                                 gCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATgc                      <  2:1727970/150‑1 (MQ=255)
                                                                                                                                  cGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATgcg                     >  1:4523855/1‑150 (MQ=255)
                                                                                                                                  cGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATgcg                     >  2:3215190/1‑150 (MQ=255)
                                                                                                                                  cGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATgcg                     >  2:2841080/1‑150 (MQ=255)
                                                                                                                                    aaaaaCTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGcgcc                   <  1:3987562/150‑1 (MQ=255)
                                                                                                                                       aaCTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCt                >  1:2282682/1‑150 (MQ=255)
                                                                                                                                        aCTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTc               <  1:892512/150‑1 (MQ=255)
                                                                                                                                        aCTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCCGGTTGCGACCGTTATCCCCTATTTTTAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTc               >  2:3309804/1‑150 (MQ=255)
                                                                                                                                        aCTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTc               >  2:2115724/1‑150 (MQ=255)
                                                                                                                                        aCTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTc               <  2:3696060/150‑1 (MQ=255)
                                                                                                                                          tctGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTcga             <  2:1935587/150‑1 (MQ=255)
                                                                                                                                             gCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTcgacga          <  1:1536541/150‑1 (MQ=255)
                                                                                                                                             gCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTcgacga          >  2:2633594/1‑150 (MQ=255)
                                                                                                                                              cccTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGaa         >  1:2351858/1‑150 (MQ=255)
                                                                                                                                                cTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCGTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGt       <  1:2067441/150‑1 (MQ=255)
                                                                                                                                                     aaaTTGACAAGACGCCCTACAAAGTATGGCTCTCAGACGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTcc  >  2:3115899/1‑150 (MQ=255)
                                                                                                                                                     aaaTTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTcc  <  1:2411684/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGCGGAAAGTTCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCC  >  CP009273/3096296‑3096594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑