Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,099,851 C→A 12.1% S260* (TCG→TAG)  nupG → nucleoside transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,099,8510CA12.1% 1071.9 / 80.5 306S260* (TCG→TAG) nupGnucleoside transporter
Reads supporting (aligned to +/- strand):  ref base C (139/130);  new base A (20/17);  total (159/147)
Fisher's exact test for biased strand distribution p-value = 8.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 306 total aligned reads displayed.

GCAATCTTCTTTATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTA  >  CP009273/3099703‑3099999
                                                                                                                                                    |                                                                                                                                                    
gCAATCTTCTTTATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCg                                                                                                                                                     >  1:2776455/1‑150 (MQ=255)
  aaTCTTCTTTATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCa                                                                                                                                                   >  1:881375/1‑150 (MQ=255)
  aaTCTTCTTTATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCa                                                                                                                                                   >  1:3883454/1‑150 (MQ=255)
  aaTCTTCTTTATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCa                                                                                                                                                   <  2:3145485/150‑1 (MQ=255)
  aaTCTTCTTTATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCa                                                                                                                                                   >  1:4438924/1‑150 (MQ=255)
   aTCTTCTTTATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAg                                                                                                                                                  <  2:4490186/150‑1 (MQ=255)
   aTCTTCTTTATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAg                                                                                                                                                  >  2:1390375/1‑150 (MQ=255)
     cttcttTATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGAt                                                                                                                                                >  2:3200380/1‑150 (MQ=255)
      ttcttTATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGAtc                                                                                                                                               <  2:329249/150‑1 (MQ=255)
      ttcttTATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGAtc                                                                                                                                               >  2:441244/1‑150 (MQ=255)
      ttcttTATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTAGCAGAtc                                                                                                                                               >  1:989319/1‑150 (MQ=255)
         tttATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATctct                                                                                                                                            >  1:319697/1‑150 (MQ=255)
         tttATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTAGCAGATctct                                                                                                                                            >  1:3013555/1‑150 (MQ=255)
                tctcAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAAccc                                                                                                                                     >  1:4333929/1‑150 (MQ=255)
                 ctcAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCt                                                                                                                                    <  2:2513704/150‑1 (MQ=255)
                    aaTGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTAGCAGATCTCTGAAACCCTGtt                                                                                                                                 >  1:3252843/1‑150 (MQ=255)
                       gctgctGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCAt                                                                                                                              <  2:303001/150‑1 (MQ=255)
                       gctgctGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCAt                                                                                                                              <  2:1978953/150‑1 (MQ=255)
                       gctgctGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTAGCAGATCTCTGAAACCCTGTTCAt                                                                                                                              <  2:97204/150‑1 (MQ=255)
                        ctgctgGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCAtt                                                                                                                             <  1:3048795/150‑1 (MQ=255)
                         tgctgGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTc                                                                                                                            <  2:4223781/150‑1 (MQ=255)
                         tgctgGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTc                                                                                                                            <  2:4587351/150‑1 (MQ=255)
                          gctgGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCt                                                                                                                           <  1:2255494/150‑1 (MQ=255)
                                  cgGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGGCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATcc                                                                                                                   >  1:3479992/1‑150 (MQ=255)
                                   ggAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATccc                                                                                                                  >  1:896036/1‑150 (MQ=255)
                                   ggAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATccc                                                                                                                  >  1:1947254/1‑150 (MQ=255)
                                     aaCTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGt                                                                                                                >  1:1193384/1‑150 (MQ=255)
                                      aCTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTAGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGtt                                                                                                               >  1:4593458/1‑150 (MQ=255)
                                         gCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGttctt                                                                                                            <  1:2007594/150‑1 (MQ=255)
                                            gATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTcttctt                                                                                                         <  1:3957146/150‑1 (MQ=255)
                                             aTTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTa                                                                                                        <  2:1277519/150‑1 (MQ=255)
                                              ttACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTaa                                                                                                       >  1:2055774/1‑150 (MQ=255)
                                               tACCAACATGTTCGGTAATAACTTCCTGCACAGCTTCGACAAAGATCCCATTTATGCCAGCAGCTTTATTGTGCATCATGCGTCAATAAACATTACGATTTCGCAGATCTCTGAAACCCAGTTCATTCTGACCATCCCGATCTTCTTaac                                                                                                      >  1:1548722/1‑149 (MQ=255)
                                                 ccAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGcc                                                                                                    >  1:167547/1‑150 (MQ=255)
                                                  cAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCg                                                                                                   <  1:3112674/150‑1 (MQ=255)
                                                     cATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTa                                                                                                >  2:2075333/1‑150 (MQ=255)
                                                      aTGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTAc                                                                                               >  1:1877706/1‑150 (MQ=255)
                                                       tGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGAAGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCAAGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCAACCCGTTCTTCTTAAGCCGCTACg                                                                                              >  1:4101142/1‑150 (MQ=255)
                                                          tCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTAGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTa                                                                                           >  1:4516203/1‑150 (MQ=255)
                                                             gTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTa                                                                                        >  2:659884/1‑150 (MQ=255)
                                                              tAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTaa                                                                                       >  1:2104924/1‑150 (MQ=255)
                                                               aaTACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAg                                                                                      >  2:2871158/1‑150 (MQ=255)
                                                                 tACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGaa                                                                                    <  2:110165/150‑1 (MQ=255)
                                                                  aCCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAAc                                                                                   <  2:497288/150‑1 (MQ=255)
                                                                  aCCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAAc                                                                                   >  1:2193248/1‑150 (MQ=255)
                                                                    cTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGt                                                                                 <  1:3535883/150‑1 (MQ=255)
                                                                      tCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTAGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTaa                                                                               <  2:4184700/150‑1 (MQ=255)
                                                                       ccTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTAGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAat                                                                              <  2:3638751/150‑1 (MQ=255)
                                                                          gCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAatgat                                                                           <  2:3441219/150‑1 (MQ=255)
                                                                            acaGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAAtgatga                                                                         >  2:2922358/1‑150 (MQ=255)
                                                                              aGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATc                                                                       >  2:3559052/1‑150 (MQ=255)
                                                                              aGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATc                                                                       <  2:691561/150‑1 (MQ=255)
                                                                              aGCTTCGACAAAGATCCGATGTTTGCCAGAAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATc                                                                       >  1:1885440/1‑150 (MQ=255)
                                                                                cTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAg                                                                     <  1:763042/150‑1 (MQ=255)
                                                                                 ttCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGt                                                                    >  2:2577986/1‑150 (MQ=255)
                                                                                  tCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTa                                                                   <  2:2311174/150‑1 (MQ=255)
                                                                                  tCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTa                                                                   >  1:3696513/1‑150 (MQ=255)
                                                                                  tCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTa                                                                   <  1:4410937/150‑1 (MQ=255)
                                                                                    gACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTAtt                                                                 >  2:117672/1‑150 (MQ=255)
                                                                                      cAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTAGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATtgt                                                               <  2:2500760/150‑1 (MQ=255)
                                                                                         aGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGc                                                            <  1:1184417/150‑1 (MQ=255)
                                                                                             ccGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTgg                                                        <  2:752002/150‑1 (MQ=255)
                                                                                                aTGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATc                                                     >  2:2831993/1‑150 (MQ=255)
                                                                                                  gTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCt                                                   <  2:4209931/150‑1 (MQ=255)
                                                                                                   tttGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTAGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTg                                                  >  2:3729361/1‑150 (MQ=255)
                                                                                                   tttGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTAGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTg                                                  <  1:55388/150‑1 (MQ=255)
                                                                                                     tGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCg                                                >  1:1436321/1‑150 (MQ=255)
                                                                                                          gcagcTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTg                                           >  1:1808229/1‑150 (MQ=255)
                                                                                                          gcagcTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTg                                           >  1:2790348/1‑150 (MQ=255)
                                                                                                              cTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGTGCt                                       >  2:1844563/1‑150 (MQ=255)
                                                                                                              cTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTAGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCt                                       <  1:263208/150‑1 (MQ=255)
                                                                                                               tttATTGTGCAGCATGCGTCAATCATCATGTCGATTTAGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTg                                      >  1:2387903/1‑150 (MQ=255)
                                                                                                                ttATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGt                                     <  2:4294889/150‑1 (MQ=255)
                                                                                                                    tgtgCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAATAACGAAATGATGATCAGTATTGTTGCGTGGATCCTGCGTTTTGATCTGTTTGc                                 >  1:4045784/1‑150 (MQ=255)
                                                                                                                    tgtgCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGc                                 >  2:568628/1‑150 (MQ=255)
                                                                                                                       gcagcaTGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTa                              <  2:2223216/150‑1 (MQ=255)
                                                                                                                         agcaTGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACg                            <  1:3853117/150‑1 (MQ=255)
                                                                                                                            aTGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCg                         <  1:2917054/150‑1 (MQ=255)
                                                                                                                              gCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGAc                       <  2:881375/150‑1 (MQ=255)
                                                                                                                              gCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGAc                       <  2:3883446/150‑1 (MQ=255)
                                                                                                                               cGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGAcc                      >  1:606951/1‑150 (MQ=255)
                                                                                                                                gTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGAccc                     >  2:1576846/1‑150 (MQ=255)
                                                                                                                                     tcatcaTGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTc                >  2:2146156/1‑150 (MQ=255)
                                                                                                                                      catcatGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTcc               <  2:4105685/150‑1 (MQ=255)
                                                                                                                                       atcatGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCg              <  2:3107373/150‑1 (MQ=255)
                                                                                                                                       atcatGTCGATTTAGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCg              <  1:687928/150‑1 (MQ=255)
                                                                                                                                       atcatGTCGATTTAGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCg              <  1:688010/150‑1 (MQ=255)
                                                                                                                                        tcatGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGt             >  2:1948382/1‑150 (MQ=255)
                                                                                                                                         catGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGtt            <  2:1158997/150‑1 (MQ=255)
                                                                                                                                         catGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGtt            <  2:1685287/150‑1 (MQ=255)
                                                                                                                                           tGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCg          <  1:1015890/150‑1 (MQ=255)
                                                                                                                                           tGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCg          >  2:145559/1‑150 (MQ=255)
                                                                                                                                            gTCGATTTCGCAGATCTCTGAATCCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCgg         <  2:61668/150‑1 (MQ=255)
                                                                                                                                            gTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCgg         >  1:1394168/1‑150 (MQ=255)
                                                                                                                                            gTCGATTTAGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCgg         >  2:22549/1‑150 (MQ=255)
                                                                                                                                              cGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGgta       <  2:3832815/150‑1 (MQ=255)
                                                                                                                                                aTTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGgtact     <  1:4233615/150‑1 (MQ=255)
                                                                                                                                                aTTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGgtact     >  2:3520108/1‑150 (MQ=255)
                                                                                                                                                 tttCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGgtactg    <  1:4269433/150‑1 (MQ=255)
                                                                                                                                                   tCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGgtactgta  >  2:2669775/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCAATCTTCTTTATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAAGATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTTCATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTA  >  CP009273/3099703‑3099999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑