Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,110,751 C→A 100% R574L (CGT→CTT)  yghJ ← DUF4092 family putative lipoprotein peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,110,7510CA96.0% 789.5 / 19.4 256R574L (CGT→CTT) yghJDUF4092 family putative lipoprotein peptidase
Reads supporting (aligned to +/- strand):  ref base C (3/8);  new base A (126/117);  total (129/127)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 258 total aligned reads displayed.

TGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTA  >  CP009273/3110605‑3110896
                                                                                                                                                  |                                                                                                                                                 
tGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCa                                                                                                                                                <  1:3812984/150‑1 (MQ=255)
    tttCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAAt                                                                                                                                            >  1:976530/1‑150 (MQ=255)
    tttCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCATACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAAt                                                                                                                                            <  1:2567729/150‑1 (MQ=255)
       cgcgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCt                                                                                                                                         <  1:4492663/150‑1 (MQ=255)
        gcgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtg                                                                                                                                        <  1:3783755/150‑1 (MQ=255)
         cgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgt                                                                                                                                       >  2:3622445/1‑150 (MQ=255)
         cgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgt                                                                                                                                       >  2:4135075/1‑150 (MQ=255)
            ccGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgttgt                                                                                                                                    >  1:3140723/1‑150 (MQ=255)
             cGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTa                                                                                                                                   >  2:914068/1‑150 (MQ=255)
                      tttATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGtt                                                                                                                          >  1:3896214/1‑150 (MQ=255)
                      tttATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGtt                                                                                                                          <  2:4224036/150‑1 (MQ=255)
                         aTCGTCGGACAGATAGCGCAGCACGTTCTCTATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTAcc                                                                                                                       <  1:4183658/150‑1 (MQ=255)
                         aTCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTAcc                                                                                                                       <  2:2320549/150‑1 (MQ=255)
                            gtcgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGAt                                                                                                                    <  2:3258009/150‑1 (MQ=255)
                             tcgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATa                                                                                                                   <  1:143643/150‑1 (MQ=255)
                               ggACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAAc                                                                                                                 <  1:2387205/150‑1 (MQ=255)
                                 aCAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCa                                                                                                               <  1:1024080/150‑1 (MQ=255)
                                    gATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCa                                                                                                            <  2:881869/150‑1 (MQ=255)
                                     aTAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAATGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAg                                                                                                           <  1:2696949/150‑1 (MQ=255)
                                      tAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGt                                                                                                          >  2:801824/1‑150 (MQ=255)
                                       aGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGtt                                                                                                         >  1:2885169/1‑150 (MQ=255)
                                         cgcAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTg                                                                                                       <  2:205899/150‑1 (MQ=255)
                                           cagcaCGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGcc                                                                                                     >  2:2540686/1‑150 (MQ=255)
                                                 gTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCAcc                                                                                               >  1:1976144/1‑150 (MQ=255)
                                                 gTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCAcc                                                                                               >  2:2837931/1‑150 (MQ=255)
                                                     tcCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACt                                                                                           >  1:568772/1‑150 (MQ=255)
                                                     tcCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACt                                                                                           >  1:4524717/1‑150 (MQ=255)
                                                       cATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTg                                                                                         >  1:2293251/1‑150 (MQ=255)
                                                            aGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCa                                                                                    >  1:2886565/1‑150 (MQ=255)
                                                            aGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCa                                                                                    <  2:376453/150‑1 (MQ=255)
                                                            aGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCa                                                                                    <  1:1535662/150‑1 (MQ=255)
                                                             gTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAg                                                                                   <  2:1663567/150‑1 (MQ=255)
                                                               tcttcATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCg                                                                                 >  2:4296440/1‑150 (MQ=255)
                                                                cttcATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGa                                                                                >  2:2707566/1‑150 (MQ=255)
                                                                   cATGTCATCCGGGTCGCTGTTGAGCGTACTCTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAAt                                                                             <  1:1716509/150‑1 (MQ=255)
                                                                    aTGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATa                                                                            <  2:644312/150‑1 (MQ=255)
                                                                           ccGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCa                                                                     >  1:596036/1‑150 (MQ=255)
                                                                               gTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGtt                                                                 >  2:3217119/1‑150 (MQ=255)
                                                                                tCGCTGTTGAGCGTACACTGCCCATCTTTATTACCGCCCCCGTTCCAGCTGTAACCGTTCGGGAAAAGCAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCTGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTa                                                                <  1:164892/150‑1 (MQ=255)
                                                                                  gCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTaaa                                                              >  2:1726320/1‑150 (MQ=255)
                                                                                   cTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAg                                                             <  1:1357635/150‑1 (MQ=255)
                                                                                    tGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAgg                                                            >  2:3465206/1‑150 (MQ=255)
                                                                                    tGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAgg                                                            >  2:3573094/1‑150 (MQ=255)
                                                                                    tGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAgg                                                            >  2:3939572/1‑150 (MQ=255)
                                                                                    tGTTGAGCGTACACGGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCGGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAgg                                                            <  2:1989644/150‑1 (MQ=255)
                                                                                       tGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTgg                                                         <  2:4326932/150‑1 (MQ=255)
                                                                                        gAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGc                                                        >  2:1762685/1‑150 (MQ=255)
                                                                                         aGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCg                                                       <  2:247876/150‑1 (MQ=255)
                                                                                         aGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCg                                                       >  1:1327638/1‑150 (MQ=255)
                                                                                         aGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCg                                                       <  2:581957/150‑1 (MQ=255)
                                                                                         aGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAAAGGCAGGTTAAAGGTGGCg                                                       >  2:2916714/1‑150 (MQ=255)
                                                                                           cGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGt                                                     >  2:2741072/1‑150 (MQ=255)
                                                                                             tACACGGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTAt                                                   <  2:4440241/150‑1 (MQ=255)
                                                                                               cacTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATcg                                                 >  1:68283/1‑150 (MQ=255)
                                                                                                 cTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATcgcg                                               <  1:252171/150‑1 (MQ=255)
                                                                                                  tGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATcgcgc                                              >  1:4448419/1‑150 (MQ=255)
                                                                                                     ccATATTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTa                                           <  2:3698531/150‑1 (MQ=255)
                                                                                                      cATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTaa                                          >  1:3187512/1‑150 (MQ=255)
                                                                                                      aaTCTTTTTTAACTCCCCCGTTCCAGCTGTAACCTTTCGGGCAAATAAAAATGCTGTTTTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTaa                                          <  2:1457421/149‑1 (MQ=255)
                                                                                                       aTCTTTATTACCGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAAc                                         <  1:1397925/150‑1 (MQ=255)
                                                                                                          tttATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGtt                                      >  1:36602/1‑150 (MQ=255)
                                                                                                           ttattcACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGttt                                     <  1:727781/150‑1 (MQ=255)
                                                                                                           ttattaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGttt                                     >  1:1118861/1‑150 (MQ=255)
                                                                                                             attaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTc                                   >  2:1210200/1‑150 (MQ=255)
                                                                                                              ttcaCGCCCCCGTACCAGCTGTAACCGTTCGGGCTAATCTATATGCTGTTGTAGTGTGGGTTACCGATAACCATAAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTcc                                  <  1:1513500/147‑1 (MQ=255)
                                                                                                                aaCGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCgg                                >  1:2254077/1‑150 (MQ=255)
                                                                                                                 aCGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGc                               >  1:4017338/1‑150 (MQ=255)
                                                                                                                  cGCCCCCGTTCCAGCTTTAACCGTTCGGGAAACTCAAAATGGTGTTGTTGTGGGGTTTACCTATAACCTTCAGTTTGCCCTCACCGACTTGCCCAAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCt                              <  1:1930398/150‑1 (MQ=255)
                                                                                                                  cGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCt                              <  1:3843757/150‑1 (MQ=255)
                                                                                                                  cGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGAAAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCt                              >  2:2667246/1‑150 (MQ=255)
                                                                                                                   gCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTc                             >  1:2788268/1‑150 (MQ=255)
                                                                                                                   gCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTc                             >  1:4114577/1‑150 (MQ=255)
                                                                                                                    cccccGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTca                            >  1:3029001/1‑150 (MQ=255)
                                                                                                                    cccccGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTca                            <  2:4632665/150‑1 (MQ=255)
                                                                                                                         gTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAag                       >  1:4537144/1‑150 (MQ=255)
                                                                                                                           tCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAagag                     <  1:1574967/150‑1 (MQ=255)
                                                                                                                            ccAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAgaga                    >  1:4301620/1‑150 (MQ=255)
                                                                                                                            ccAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAgaga                    <  2:1368533/150‑1 (MQ=255)
                                                                                                                              aGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAg                  >  1:898433/1‑150 (MQ=255)
                                                                                                                              aGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAg                  >  2:3023854/1‑150 (MQ=255)
                                                                                                                              aGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAg                  <  2:2123396/150‑1 (MQ=255)
                                                                                                                              aGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAg                  <  1:2648476/150‑1 (MQ=255)
                                                                                                                              aGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAg                  <  1:3305956/150‑1 (MQ=255)
                                                                                                                                cTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGgc                <  1:1686133/150‑1 (MQ=255)
                                                                                                                                 tGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGgcg               >  2:199001/1‑150 (MQ=255)
                                                                                                                                   tAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCt             >  2:182293/1‑150 (MQ=255)
                                                                                                                                    aaCCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCtt            <  1:277174/150‑1 (MQ=255)
                                                                                                                                    aaCCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCtt            <  2:4014746/150‑1 (MQ=255)
                                                                                                                                    aaCCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCtt            <  2:1251873/150‑1 (MQ=255)
                                                                                                                                    aaCCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCtt            <  2:1789995/150‑1 (MQ=255)
                                                                                                                                      ccGTTCGGTCAAAGCATAATGTTGTTTTAGTGTGGGTTACCGATAACCATCATTTTGCCATCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTcc          <  2:2449115/150‑1 (MQ=255)
                                                                                                                                      ccGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTcc          <  2:1618104/150‑1 (MQ=255)
                                                                                                                                        gTTCGGGCAACGCAAAATGCTTTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGt        <  2:3606266/150‑1 (MQ=255)
                                                                                                                                         ttCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTa       <  2:1024024/150‑1 (MQ=255)
                                                                                                                                           cGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCTATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAt     <  1:1616639/150‑1 (MQ=255)
                                                                                                                                           cGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAt     <  2:3657443/150‑1 (MQ=255)
                                                                                                                                           cGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAt     >  1:34361/1‑150 (MQ=255)
                                                                                                                                           cGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAt     >  1:1316592/1‑150 (MQ=255)
                                                                                                                                            gggCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATg    <  1:231482/150‑1 (MQ=255)
                                                                                                                                             ggCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGt   >  1:4050921/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTA  >  CP009273/3110605‑3110896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑