Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,142,668 G→T 89.3% L55I (CTC→ATC)  yqhA ← UPF0114 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,142,6680GT89.3% 728.1 / 87.1 271L55I (CTC→ATC) yqhAUPF0114 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (12/17);  new base T (130/112);  total (142/129)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 272 total aligned reads displayed.

CGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCATT  >  CP009273/3142522‑3142812
                                                                                                                                                  |                                                                                                                                                
 gTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATc                                                                                                                                              <  1:101734/150‑1 (MQ=255)
 gTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATc                                                                                                                                              >  1:3786541/1‑150 (MQ=255)
  ttGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCa                                                                                                                                             >  1:1714589/1‑150 (MQ=255)
   tGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCaa                                                                                                                                            >  1:113308/1‑150 (MQ=255)
     cGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAAt                                                                                                                                          <  2:1560426/150‑1 (MQ=255)
      gTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATc                                                                                                                                         >  2:241769/1‑150 (MQ=255)
         cATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGa                                                                                                                                      >  1:3627333/1‑150 (MQ=255)
            tttCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTc                                                                                                                                   <  1:1412785/150‑1 (MQ=255)
             ttCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTcc                                                                                                                                  <  2:3582597/150‑1 (MQ=255)
             ttCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTcc                                                                                                                                  <  1:1169626/150‑1 (MQ=255)
              tCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCg                                                                                                                                 >  2:1349339/1‑150 (MQ=255)
                    gccagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCg                                                                                                                           >  1:3250095/1‑150 (MQ=255)
                     ccagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCga                                                                                                                          >  2:4105196/1‑150 (MQ=255)
                      cagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgag                                                                                                                         >  1:803152/1‑150 (MQ=255)
                      cagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgag                                                                                                                         >  1:803179/1‑150 (MQ=255)
                      cagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgag                                                                                                                         >  1:995212/1‑150 (MQ=255)
                      cagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgag                                                                                                                         >  1:1307685/1‑150 (MQ=255)
                      cagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgag                                                                                                                         >  2:388311/1‑150 (MQ=255)
                         ttCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGagaag                                                                                                                      >  2:1600616/1‑150 (MQ=255)
                          tCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGagaaga                                                                                                                     <  2:3807264/150‑1 (MQ=255)
                           cAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGat                                                                                                                    <  1:584362/150‑1 (MQ=255)
                               ttCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTc                                                                                                                <  2:2766505/150‑1 (MQ=255)
                                tctcTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCg                                                                                                               <  2:2326103/150‑1 (MQ=255)
                                tctcTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCg                                                                                                               <  1:415557/150‑1 (MQ=255)
                                tctcTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCg                                                                                                               <  2:4311182/150‑1 (MQ=255)
                                    tttGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGG‑AAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGt                                                                                                          >  1:4502448/1‑150 (MQ=255)
                                     ttGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGt                                                                                                          <  1:2388896/150‑1 (MQ=255)
                                       gTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTAc                                                                                                        >  2:645092/1‑150 (MQ=255)
                                           tcGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGa                                                                                                    >  1:2305498/1‑150 (MQ=255)
                                           tcGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGa                                                                                                    >  1:3957023/1‑150 (MQ=255)
                                            cGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaa                                                                                                   <  2:4084633/150‑1 (MQ=255)
                                            cGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaa                                                                                                   <  1:4188803/150‑1 (MQ=255)
                                             ggAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaat                                                                                                  >  1:2773118/1‑150 (MQ=255)
                                                    tCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTcc                                                                                           <  2:1641109/150‑1 (MQ=255)
                                                      cAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTg                                                                                         >  1:4498234/1‑150 (MQ=255)
                                                       aGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTgg                                                                                        >  1:4334841/1‑150 (MQ=255)
                                                        gCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGga                                                                                       <  2:1031152/150‑1 (MQ=255)
                                                         cTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGgaa                                                                                      <  2:1241184/150‑1 (MQ=255)
                                                         cTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGgaa                                                                                      >  1:918996/1‑150 (MQ=255)
                                                          tgtgAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGgaag                                                                                     <  2:309993/150‑1 (MQ=255)
                                                          tgtgAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGgaag                                                                                     <  1:4634764/150‑1 (MQ=255)
                                                           gtgAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGgaaga                                                                                    >  1:3053504/1‑150 (MQ=255)
                                                            tgAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGAGAATAATCTCCTGgaagaa                                                                                   >  1:4576719/1‑150 (MQ=255)
                                                                aCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTc                                                                               <  2:190563/150‑1 (MQ=255)
                                                                aCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTc                                                                               >  1:2068488/1‑150 (MQ=255)
                                                                       tctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCa                                                                        <  1:3735396/150‑1 (MQ=255)
                                                                       tctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCa                                                                        <  1:3458031/150‑1 (MQ=255)
                                                                        ctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAg                                                                       <  1:3667033/150‑1 (MQ=255)
                                                                         tcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAgg                                                                      <  2:1725293/150‑1 (MQ=255)
                                                                           aTAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGc                                                                    >  1:2833951/1‑150 (MQ=255)
                                                                             aaCCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCaa                                                                  >  1:3315456/1‑150 (MQ=255)
                                                                                 ggAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTaa                                                              >  2:4562968/1‑150 (MQ=255)
                                                                                  gAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAAc                                                             >  1:3487105/1‑150 (MQ=255)
                                                                                      aCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCa                                                         <  1:1613437/150‑1 (MQ=255)
                                                                                      aCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCa                                                         <  1:1953679/150‑1 (MQ=255)
                                                                                      aCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCa                                                         <  1:2987363/150‑1 (MQ=255)
                                                                                      aCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCa                                                         <  1:2991544/150‑1 (MQ=255)
                                                                                       catcaccatcacCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACCAACGCCAg                                                        <  2:4420386/150‑1 (MQ=255)
                                                                                            ccatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGaaa                                                   >  2:4544650/1‑150 (MQ=255)
                                                                                              atcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAgg                                                 <  2:3550708/150‑1 (MQ=255)
                                                                                              atcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAgg                                                 >  2:3641453/1‑150 (MQ=255)
                                                                                                caccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGcc                                               <  1:2205256/150‑1 (MQ=255)
                                                                                                 accaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCa                                              >  1:648385/1‑150 (MQ=255)
                                                                                                    aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAGCACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCACCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAg                                           >  1:785616/1‑150 (MQ=255)
                                                                                                    aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAg                                           <  2:1209138/150‑1 (MQ=255)
                                                                                                    aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAg                                           >  1:859029/1‑150 (MQ=255)
                                                                                                    aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAg                                           >  1:70782/1‑150 (MQ=255)
                                                                                                    aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAg                                           <  1:4058746/150‑1 (MQ=255)
                                                                                                    aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACATGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAg                                           >  2:263291/1‑150 (MQ=255)
                                                                                                       aaaCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTac                                        >  1:1038606/1‑150 (MQ=255)
                                                                                                       aaaCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTac                                        >  2:1108175/1‑150 (MQ=255)
                                                                                                        aaCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTaca                                       >  2:289909/1‑150 (MQ=255)
                                                                                                        aaCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTaca                                       <  1:3383098/150‑1 (MQ=255)
                                                                                                        aaCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTaca                                       >  2:2108652/1‑150 (MQ=255)
                                                                                                           cGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACgg                                    >  2:3700612/1‑150 (MQ=255)
                                                                                                               aaCCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGc                                >  1:2610906/1‑150 (MQ=255)
                                                                                                               aaCCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGc                                >  2:3975137/1‑150 (MQ=255)
                                                                                                               aaCCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGc                                >  2:1260371/1‑150 (MQ=255)
                                                                                                                 ccAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCaa                              <  2:672187/150‑1 (MQ=255)
                                                                                                                   aGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCTGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagc                            <  1:1175680/150‑1 (MQ=255)
                                                                                                                    gCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagca                           <  2:2777021/150‑1 (MQ=255)
                                                                                                                     cGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagcag                          <  2:3315593/150‑1 (MQ=255)
                                                                                                                      gTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagcagc                         >  1:1947091/1‑150 (MQ=255)
                                                                                                                      gTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagcagc                         >  1:3574993/1‑150 (MQ=255)
                                                                                                                        cATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGcagcca                       >  2:534374/1‑150 (MQ=255)
                                                                                                                        cATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGcagcca                       >  1:2713514/1‑150 (MQ=255)
                                                                                                                         atatCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGcagccag                      <  1:2486471/150‑1 (MQ=255)
                                                                                                                         atatCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGcagccag                      <  2:932192/150‑1 (MQ=255)
                                                                                                                          tatCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGcagccagc                     >  1:300885/1‑150 (MQ=255)
                                                                                                                            tCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCga                   >  1:320034/1‑150 (MQ=255)
                                                                                                                            tCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCga                   <  1:2584706/150‑1 (MQ=255)
                                                                                                                             ccaccaGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCgag                  <  2:1789124/150‑1 (MQ=255)
                                                                                                                                 caGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGc              >  1:3808074/1‑150 (MQ=255)
                                                                                                                                   gCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCAt            <  2:1328304/150‑1 (MQ=255)
                                                                                                                                       cAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACAt        <  2:2486214/150‑1 (MQ=255)
                                                                                                                                         gCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTg      >  2:3763366/1‑150 (MQ=255)
                                                                                                                                         gCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTg      <  2:181194/150‑1 (MQ=255)
                                                                                                                                            acacGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCAt   <  2:3004020/150‑1 (MQ=255)
                                                                                                                                            acacGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCAt   <  2:846911/150‑1 (MQ=255)
                                                                                                                                             cacGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCAtt  <  2:1410844/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCATT  >  CP009273/3142522‑3142812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑