Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,373,985 C→A 100% intergenic (+37/‑117) yhcB → / → degQ DUF1043 family inner membrane‑anchored protein/serine endoprotease, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,373,9850CA96.9% 1008.7 / 18.8 322intergenic (+37/‑117)yhcB/degQDUF1043 family inner membrane‑anchored protein/serine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (2/9);  new base A (117/194);  total (119/203)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 337 total aligned reads displayed.

AATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTGCA  >  CP009273/3373838‑3374131
                                                                                                                                                   |                                                                                                                                                  
aaTCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTtatc                                                                                                                                                  >  1:3771208/1‑150 (MQ=255)
  tCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTtatctc                                                                                                                                                >  1:4621854/1‑150 (MQ=255)
    gtatGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATctccc                                                                                                                                              >  2:3541640/4‑150 (MQ=255)
         gCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTcccc                                                                                                                                         >  1:1364910/1‑150 (MQ=255)
         gCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTAATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTcccc                                                                                                                                         <  1:890396/150‑1 (MQ=255)
           agagTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGa                                                                                                                                       <  2:4129784/150‑1 (MQ=255)
             agTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACt                                                                                                                                     <  2:2443362/150‑1 (MQ=255)
                 tGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCa                                                                                                                                 <  1:4335404/150‑1 (MQ=255)
                   aaGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCAtt                                                                                                                               >  1:3035378/1‑150 (MQ=255)
                           aaCGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTgg                                                                                                                       >  2:2899150/1‑150 (MQ=255)
                           aaCGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTgg                                                                                                                       >  2:2600621/1‑150 (MQ=255)
                            aCGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGgt                                                                                                                      <  1:1509842/150‑1 (MQ=255)
                             cGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGgtg                                                                                                                     <  1:1512624/150‑1 (MQ=255)
                             cGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTAACTCCCTCCCCGACTATCATTTAATCTGgtg                                                                                                                     <  1:4291195/150‑1 (MQ=255)
                              gATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGgtgt                                                                                                                    >  1:2717687/1‑150 (MQ=255)
                               aTCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGtc                                                                                                                   >  1:977231/1‑150 (MQ=255)
                                  aGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCa                                                                                                                >  2:2640201/1‑150 (MQ=255)
                                    gCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCAtt                                                                                                              >  2:792049/1‑150 (MQ=255)
                                     cACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTg                                                                                                             <  1:3270018/150‑1 (MQ=255)
                                        cGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTa                                                                                                          >  1:1298312/1‑150 (MQ=255)
                                        cGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTa                                                                                                          >  1:1763888/1‑150 (MQ=255)
                                        cGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTa                                                                                                          >  1:4095067/1‑150 (MQ=255)
                                         ggTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAg                                                                                                         <  1:3481/150‑1 (MQ=255)
                                           tGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGcc                                                                                                       <  2:2584893/150‑1 (MQ=255)
                                            gCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCg                                                                                                      <  2:628959/150‑1 (MQ=255)
                                              aGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTc                                                                                                    >  1:602842/1‑150 (MQ=255)
                                               gATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCt                                                                                                   <  1:2621627/150‑1 (MQ=255)
                                               gATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCt                                                                                                   <  2:3111118/150‑1 (MQ=255)
                                                aTGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTg                                                                                                  <  2:4258224/150‑1 (MQ=255)
                                                 tGCCTCGCGACTATTCTGACGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGa                                                                                                 <  2:4071253/150‑1 (MQ=255)
                                                 tGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGa                                                                                                 <  2:4071462/150‑1 (MQ=255)
                                                 tGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGa                                                                                                 <  1:3932827/150‑1 (MQ=255)
                                                   ccTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGaaa                                                                                               <  2:1560559/150‑1 (MQ=255)
                                                   ccTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGaaa                                                                                               <  2:1089354/150‑1 (MQ=255)
                                                    cTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGaaaa                                                                                              <  2:4196027/150‑1 (MQ=255)
                                                       gcgACTAGTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTc                                                                                           <  2:3244995/150‑1 (MQ=255)
                                                           cTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATa                                                                                       >  2:4440012/1‑150 (MQ=255)
                                                           cTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATa                                                                                       <  1:3076980/150‑1 (MQ=255)
                                                             aTTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAAc                                                                                     >  2:3935826/1‑150 (MQ=255)
                                                              ttCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACa                                                                                    <  1:3994329/150‑1 (MQ=255)
                                                               tCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACAt                                                                                   <  1:2132293/150‑1 (MQ=255)
                                                                  gAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCaa                                                                                >  2:2159259/1‑150 (MQ=255)
                                                                     ggCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACt                                                                             <  2:451/150‑1 (MQ=255)
                                                                      gcgcATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTg                                                                            <  2:2465080/150‑1 (MQ=255)
                                                                        gcATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGtt                                                                          <  1:2345536/150‑1 (MQ=255)
                                                                            ccGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGa                                                                      <  2:1709188/150‑1 (MQ=255)
                                                                             cGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGaa                                                                     <  1:851910/150‑1 (MQ=255)
                                                                              ggCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTTTTAGACGTCTGAAAATTCAATAACATCAAACTGTTATGAAt                                                                    >  2:3144797/1‑150 (MQ=255)
                                                                              ggCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAAt                                                                    >  2:1976253/1‑150 (MQ=255)
                                                                              ggCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAAt                                                                    >  1:1377364/1‑150 (MQ=255)
                                                                                ccTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAAtct                                                                  <  1:56934/150‑1 (MQ=255)
                                                                                  tgctgcGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATctct                                                                >  1:2567725/1‑150 (MQ=255)
                                                                                   gctgcGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCtt                                                               <  2:1426609/150‑1 (MQ=255)
                                                                                     tgcGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCtttt                                                             <  2:49634/150‑1 (MQ=255)
                                                                                     tgcGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCtttt                                                             <  1:2270088/150‑1 (MQ=255)
                                                                                      gcGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTc                                                            <  1:2518321/150‑1 (MQ=255)
                                                                                          aCTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTAt                                                        >  1:602158/1‑150 (MQ=255)
                                                                                          aCTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTAt                                                        <  2:3685028/150‑1 (MQ=255)
                                                                                           cTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATc                                                       <  2:2053047/150‑1 (MQ=255)
                                                                                            tGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCa                                                      >  2:3605777/1‑150 (MQ=255)
                                                                                             ggCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCAATTGAGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTTTTCTGGTGTCTCATTGTTAGCCGTCTTAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCAt                                                     <  1:670624/150‑1 (MQ=255)
                                                                                                gcgAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCa                                                  >  1:3866395/1‑150 (MQ=255)
                                                                                                   aaGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGt                                               >  1:3695750/1‑150 (MQ=255)
                                                                                                     gcgcGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTAc                                             <  2:294040/150‑1 (MQ=255)
                                                                                                     gcgcGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTAc                                             <  1:3340263/150‑1 (MQ=255)
                                                                                                      cgcgACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACg                                            >  1:1153499/1‑150 (MQ=255)
                                                                                                      cgcgACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCACTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACg                                            <  2:2552418/150‑1 (MQ=255)
                                                                                                       gcgACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACga                                           <  1:282303/150‑1 (MQ=255)
                                                                                                       gcgACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACga                                           >  1:1668252/1‑150 (MQ=255)
                                                                                                         gACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACgaga                                         <  1:100687/150‑1 (MQ=255)
                                                                                                          aCTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGagag                                        <  2:3264419/150‑1 (MQ=255)
                                                                                                           cTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTAAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGc                                       <  2:1515632/150‑1 (MQ=255)
                                                                                                             aaTTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTATTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAg                                     <  2:9386/150‑1 (MQ=255)
                                                                                                               tttatttTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGa                                   <  1:2405025/150‑1 (MQ=255)
                                                                                                               tttatttTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGa                                   <  2:3665911/150‑1 (MQ=255)
                                                                                                                    ttttCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCTGGTACGAGAGCAGGaataat                              >  2:4360142/1‑150 (MQ=255)
                                                                                                                    ttttCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGaataat                              <  2:1631641/150‑1 (MQ=255)
                                                                                                                       tCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGaa                           <  2:153503/150‑1 (MQ=255)
                                                                                                                       tCGGGCGCAGCCATAGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGaa                           <  2:3446106/150‑1 (MQ=255)
                                                                                                                        cGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGaaa                          >  2:3021851/1‑150 (MQ=255)
                                                                                                                         gggCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGaaaa                         >  1:1871054/1‑150 (MQ=255)
                                                                                                                             gcAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAaaaca                     >  2:1306627/1‑150 (MQ=255)
                                                                                                                             gcAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAaaaca                     <  1:357954/150‑1 (MQ=255)
                                                                                                                                  cATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAAccc                >  2:1464348/1‑150 (MQ=255)
                                                                                                                                    ttGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAg              <  1:778968/150‑1 (MQ=255)
                                                                                                                                     tGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGc             >  2:1925727/1‑150 (MQ=255)
                                                                                                                                       cgcCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTg           >  1:4534661/1‑150 (MQ=255)
                                                                                                                                        gcCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGt          >  2:963008/1‑150 (MQ=255)
                                                                                                                                         cccTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGtt         <  2:3389106/150‑1 (MQ=255)
                                                                                                                                         cccTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTggt         <  2:2976064/150‑3 (MQ=255)
                                                                                                                                         cccTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGtt         <  2:764015/150‑1 (MQ=255)
                                                                                                                                          cctcctCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTg        >  2:3093702/1‑150 (MQ=255)
                                                                                                                                          cctcctCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTg        <  1:2912754/150‑1 (MQ=255)
                                                                                                                                          cctcctCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTg        <  1:2646852/150‑1 (MQ=255)
                                                                                                                                          cctcctCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTg        <  1:112063/150‑1 (MQ=255)
                                                                                                                                               tcttatCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTGc   <  2:874906/150‑1 (MQ=255)
                                                                                                                                               tcttatCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCAATGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTGc   >  2:1848058/1‑150 (MQ=255)
                                                                                                                                                cttatCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTGCa  >  2:228270/1‑150 (MQ=255)
                                                                                                                                                cttatCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTGCa  <  1:3361902/150‑1 (MQ=255)
                                                                                                                                                cttatCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTGCa  <  1:2078914/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTGCA  >  CP009273/3373838‑3374131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑