Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,392,935 C→T 19.7% V135I (GTT→ATT)  mreC ← cell wall structural complex MreBCD transmembrane component MreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,392,9350CT19.7% 688.8 / 138.0 275V135I (GTT→ATT) mreCcell wall structural complex MreBCD transmembrane component MreC
Reads supporting (aligned to +/- strand):  ref base C (106/113);  new base T (27/27);  total (134/141)
Fisher's exact test for biased strand distribution p-value = 8.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

CTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCA  >  CP009273/3392786‑3393084
                                                                                                                                                     |                                                                                                                                                     
   gATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTg                                                                                                                                                    <  2:4401695/150‑1 (MQ=255)
   gATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTg                                                                                                                                                    <  2:3699168/150‑1 (MQ=255)
     ttGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTGAt                                                                                                                                                  >  1:939886/1‑150 (MQ=255)
     ttGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGAt                                                                                                                                                  >  2:1757442/1‑150 (MQ=255)
       ggCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTGATCg                                                                                                                                                >  2:4532341/1‑150 (MQ=255)
       ggCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTGATCg                                                                                                                                                <  1:1587711/150‑1 (MQ=255)
       ggCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCg                                                                                                                                                <  1:599689/150‑1 (MQ=255)
         cAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCt                                                                                                                                              <  2:715625/150‑1 (MQ=255)
          aGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCta                                                                                                                                             >  2:1452153/1‑150 (MQ=255)
              cgTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAg                                                                                                                                         <  1:2461503/150‑1 (MQ=255)
               gTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAgg                                                                                                                                        <  2:2798608/150‑1 (MQ=255)
                    tCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTGATCGCTATAAGGATCGt                                                                                                                                   >  1:3173994/1‑150 (MQ=255)
                    tCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGt                                                                                                                                   <  1:620193/150‑1 (MQ=255)
                     cGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTGATCGCTATAAGGATCGtt                                                                                                                                  <  2:1051056/150‑1 (MQ=255)
                     cGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGtt                                                                                                                                  <  2:1589930/150‑1 (MQ=255)
                     cGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGtt                                                                                                                                  <  2:389293/150‑1 (MQ=255)
                       cATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTaa                                                                                                                                <  1:191103/150‑1 (MQ=255)
                       cATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTaa                                                                                                                                >  2:2402996/1‑150 (MQ=255)
                        aTCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAAc                                                                                                                               <  2:3390425/150‑1 (MQ=255)
                              aaTCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGga                                                                                                                         <  2:1956642/150‑1 (MQ=255)
                              aaTCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGga                                                                                                                         <  1:4254437/150‑1 (MQ=255)
                               aTCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGgag                                                                                                                        >  2:1136606/1‑150 (MQ=255)
                               aTCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGgag                                                                                                                        <  1:1196967/150‑1 (MQ=255)
                                tCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGgaga                                                                                                                       >  1:1729617/1‑150 (MQ=255)
                                tCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGgaga                                                                                                                       <  1:3510289/150‑1 (MQ=255)
                                 cagcagCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTGATCGCTATAAGGATCGTTAACCGTGGAGAt                                                                                                                      <  2:289425/150‑1 (MQ=255)
                                 cagcagCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGAt                                                                                                                      <  2:2928133/150‑1 (MQ=255)
                                  agcagcACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTGATCGCTATAAGGATCGTTAACCGTGGAGATa                                                                                                                     <  1:4046155/150‑1 (MQ=255)
                                     agcaCGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACACCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAAcc                                                                                                                  <  1:291488/150‑1 (MQ=255)
                                       caCGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACACCACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTg                                                                                                                <  2:643377/150‑1 (MQ=255)
                                          gcgACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGt                                                                                                             <  2:3751102/150‑1 (MQ=255)
                                          gcgACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGt                                                                                                             >  2:3635926/1‑150 (MQ=255)
                                           cgACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTc                                                                                                            >  2:4288869/1‑150 (MQ=255)
                                            gACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCa                                                                                                           >  2:258007/1‑150 (MQ=255)
                                                ggTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCAt                                                                                                       >  1:1948196/1‑150 (MQ=255)
                                                  tCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCAttt                                                                                                     >  1:2641375/1‑150 (MQ=255)
                                                   cAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCAtttt                                                                                                    >  2:2849783/1‑150 (MQ=255)
                                                        tAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCt                                                                                               >  1:4404601/1‑150 (MQ=255)
                                                         aGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTc                                                                                              >  1:871503/1‑150 (MQ=255)
                                                          gCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCa                                                                                             <  1:4532341/150‑1 (MQ=255)
                                                           cGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCAt                                                                                            >  1:3279435/1‑150 (MQ=255)
                                                           cGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCAt                                                                                            >  1:4461294/1‑150 (MQ=255)
                                                            gACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATc                                                                                           >  1:4363851/1‑150 (MQ=255)
                                                            gACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATc                                                                                           >  2:3459575/1‑150 (MQ=255)
                                                            gACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATc                                                                                           >  2:3784302/1‑150 (MQ=255)
                                                            gACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATc                                                                                           >  1:2034191/1‑150 (MQ=255)
                                                             aCGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATACCAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATcc                                                                                          <  1:669503/150‑1 (MQ=255)
                                                             aCGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATcc                                                                                          <  2:3870679/150‑1 (MQ=255)
                                                              cGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCt                                                                                         <  2:2662462/150‑1 (MQ=255)
                                                                 ccaccaCCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGAc                                                                                      <  2:2300143/150‑1 (MQ=255)
                                                                  caccacCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACg                                                                                     <  2:491116/150‑1 (MQ=255)
                                                                  caccacCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACg                                                                                     >  1:3423072/1‑150 (MQ=255)
                                                                    ccaccTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCa                                                                                   <  1:3106026/150‑1 (MQ=255)
                                                                        cTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCgg                                                                               >  1:2080303/1‑150 (MQ=255)
                                                                           aCCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGa                                                                            <  2:2877278/150‑1 (MQ=255)
                                                                           aCCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGa                                                                            <  2:1539647/150‑1 (MQ=255)
                                                                              aacaacACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAAcc                                                                         >  2:2354420/1‑150 (MQ=255)
                                                                               acaacaCCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAAccc                                                                        <  2:3586375/150‑1 (MQ=255)
                                                                               acaacaCCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGAGAACACCTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAAccc                                                                        <  2:1438054/150‑1 (MQ=255)
                                                                                 aacaCCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCcag                                                                      <  2:3309251/150‑1 (MQ=255)
                                                                                            cGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCa                                                           <  1:2984804/150‑1 (MQ=255)
                                                                                               tgatgaCCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGAc                                                        <  2:1207205/150‑1 (MQ=255)
                                                                                                   gaCCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAATTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGAcgcgc                                                    >  2:2731356/1‑150 (MQ=255)
                                                                                                      cGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGtt                                                 >  1:2311692/1‑150 (MQ=255)
                                                                                                       ggCTGGCCTTCATAAACGCCATTAACGCTANCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTtc                                                >  1:4287527/1‑150 (MQ=255)
                                                                                                       ggCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTtc                                                <  1:3527819/150‑1 (MQ=255)
                                                                                                          tGGCCTTCATAAACGCCATTAACGCTACCTTTATCTATAACAACTTGAACGCTATAAGGATCCTTAACCGTTGAGAAAACCAGAGTCACCATATTCTGCTAATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTtctca                                             >  1:2354630/1‑149 (MQ=255)
                                                                                                            gCCTTCATAAACGCCATTCACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTg                                           <  1:676150/150‑1 (MQ=255)
                                                                                                            gCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTg                                           >  2:3244096/1‑150 (MQ=255)
                                                                                                             ccTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGt                                          >  2:3730764/1‑150 (MQ=255)
                                                                                                              cTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGtt                                         >  2:2915601/1‑150 (MQ=255)
                                                                                                                    aaaCGCCATTAACGCTACCTTTATCGATAACAATTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTAtt                                   >  1:3204792/1‑150 (MQ=255)
                                                                                                                    aaaCGCCATTAACGCTACCTTTATCGATAACAATTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTAtt                                   >  1:2055523/1‑150 (MQ=255)
                                                                                                                    aaaCGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTAtt                                   <  1:2894001/150‑1 (MQ=255)
                                                                                                                    aaaCGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTAtt                                   <  1:1423340/150‑1 (MQ=255)
                                                                                                                    aaaCGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATGTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTAtt                                   >  2:1876728/1‑150 (MQ=255)
                                                                                                                      aCGCCATTAACTCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCGGTTTGTATTGt                                 >  1:432097/1‑150 (MQ=255)
                                                                                                                       cGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTc                                >  2:4196133/1‑150 (MQ=255)
                                                                                                                         ccATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCa                              >  1:1380166/1‑150 (MQ=255)
                                                                                                                         ccATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCa                              >  1:3696571/1‑150 (MQ=255)
                                                                                                                          cATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCaa                             >  1:254859/1‑150 (MQ=255)
                                                                                                                           aTTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAg                            >  1:2251541/1‑150 (MQ=255)
                                                                                                                            ttAACGCTACCTTTATCGATAACAATTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGc                           <  1:278413/150‑1 (MQ=255)
                                                                                                                            ttAACGCTACCTTGATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGc                           <  1:1062543/150‑1 (MQ=255)
                                                                                                                               aCGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATc                        >  1:1007653/1‑150 (MQ=255)
                                                                                                                                cGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATca                       >  2:3938864/1‑150 (MQ=255)
                                                                                                                                 gCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATcag                      <  1:1088682/150‑1 (MQ=255)
                                                                                                                                 gCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATcag                      >  2:2135054/1‑150 (MQ=255)
                                                                                                                                  cTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATcagc                     <  2:3735376/150‑1 (MQ=255)
                                                                                                                                       tttATCGATAACAATTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCATGTTTGTATTGTCCAAGCATCAGCAGTTc                <  2:3173994/150‑1 (MQ=255)
                                                                                                                                       tttATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGGTTGTATTGTCCAAGCATCAGCAGTTc                >  2:3772673/1‑150 (MQ=255)
                                                                                                                                        ttATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCa               >  2:492989/1‑150 (MQ=255)
                                                                                                                                         tATCGATAACAATTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCAc              >  2:3016832/1‑150 (MQ=255)
                                                                                                                                          aTCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACt             <  2:2383467/150‑1 (MQ=255)
                                                                                                                                          aTCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACt             <  2:2383436/150‑1 (MQ=255)
                                                                                                                                              aTAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGttt         <  1:1400621/150‑1 (MQ=255)
                                                                                                                                                aacaacTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGttttt       <  1:644551/150‑1 (MQ=255)
                                                                                                                                                aacaacTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGttttt       >  2:1334284/1‑150 (MQ=255)
                                                                                                                                                   aacTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAg    <  1:3839209/150‑1 (MQ=255)
                                                                                                                                                    acTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGTACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGc   <  1:1493568/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCA  >  CP009273/3392786‑3393084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑