BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,432,676	C→A	13.1%	E65* (GAA→TAA)	yhdN ←	DUF1992 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,432,676	0	C	A	13.1%	882.6 / 70.8	256	E65* (GAA→TAA)	yhdN	DUF1992 family protein
Reads supporting (aligned to +/- strand): ref base C (122/100); new base A (18/16); total (140/116)
Fisher's exact test for biased strand distribution p-value = 8.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

TGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGC  >  CP009273/3432529‑3432824

tGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTtctc                                                                                                                                                    <  1:911685/150‑1 (MQ=255)

 gTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTtctct                                                                                                                                                   <  1:466800/150‑1 (MQ=255)

   cAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTGGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTcc                                                                                                                                                 <  2:3371487/150‑1 (MQ=255)

    aGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTATCTCCg                                                                                                                                                <  2:3400551/150‑1 (MQ=255)

       aTAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTg                                                                                                                                             >  1:3609496/1‑150 (MQ=255)

       aTAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTg                                                                                                                                             >  1:3609582/1‑150 (MQ=255)

        tAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGc                                                                                                                                            >  1:2642671/1‑150 (MQ=255)

        tAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGc                                                                                                                                            >  2:1582933/1‑150 (MQ=255)

        tAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGc                                                                                                                                            >  1:4514809/1‑150 (MQ=255)

         aaTCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCt                                                                                                                                           >  1:3469707/1‑150 (MQ=255)

          aTCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTc                                                                                                                                          >  2:651164/1‑150 (MQ=255)

           tCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCa                                                                                                                                         <  1:3996884/150‑1 (MQ=255)

           tCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCa                                                                                                                                         >  1:1447291/1‑150 (MQ=255)

           tCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCa                                                                                                                                         <  2:847633/150‑1 (MQ=255)

           tCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCa                                                                                                                                         <  2:875872/150‑1 (MQ=255)

            cgccgcGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCaa                                                                                                                                        >  2:3935610/1‑150 (MQ=255)

              ccgcGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGt                                                                                                                                      >  2:2245972/1‑150 (MQ=255)

               cgcgTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGtt                                                                                                                                     >  2:2092839/1‑150 (MQ=255)

               cgcgTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTATCTCCGTTGCTCAAGtt                                                                                                                                     <  2:1701438/150‑1 (MQ=255)

                gcgTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTc                                                                                                                                    <  2:1157787/150‑1 (MQ=255)

                 cgTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCt                                                                                                                                   >  2:4194264/1‑150 (MQ=255)

                  gTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTg                                                                                                                                  >  1:1227716/1‑150 (MQ=255)

                   tAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTgg                                                                                                                                 >  2:1735795/1‑150 (MQ=255)

                   tAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTgg                                                                                                                                 <  1:865185/150‑1 (MQ=255)

                    aaaaaaTCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCCAGTTCTGGc                                                                                                                                <  1:2721280/150‑1 (MQ=255)

                    aaaaaaTCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGc                                                                                                                                >  1:3817736/1‑150 (MQ=255)

                     aaaaaTCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCg                                                                                                                               <  2:4188697/150‑1 (MQ=255)

                     aaaaaTCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCg                                                                                                                               <  1:241505/150‑1 (MQ=255)

                       aaaTCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGt                                                                                                                             >  1:2020938/1‑150 (MQ=255)

                       aaaTCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGt                                                                                                                             >  2:3527124/1‑150 (MQ=255)

                       aaaTCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTATCTCCGTTGCTCAAGTTCTGGCGGt                                                                                                                             <  1:4070224/150‑1 (MQ=255)

                         aTCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTATCTCCGTTGCTCAAGTTCTGGCGGTaa                                                                                                                           <  1:4012603/150‑1 (MQ=255)

                           cGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGc                                                                                                                         >  2:3634686/1‑150 (MQ=255)

                              tACTCCATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAAc                                                                                                                      <  1:2349225/150‑1 (MQ=255)

                               aCTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAAcc                                                                                                                     >  1:2627075/1‑150 (MQ=255)

                               aCTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTATCTCCGTTGCTCAAGTTCTGGCGGTAAGCAAcc                                                                                                                     <  1:2237152/150‑1 (MQ=255)

                                   aaTCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCa                                                                                                                 <  1:4274367/150‑1 (MQ=255)

                                    aTCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCAt                                                                                                                >  1:3908147/1‑150 (MQ=255)

                                       cAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCAttct                                                                                                             <  1:3498154/150‑1 (MQ=255)

                                         gCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCAttcttc                                                                                                           <  1:664963/150‑1 (MQ=255)

                                           ttGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAg                                                                                                         >  2:3964700/1‑150 (MQ=255)

                                              tCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCaa                                                                                                      <  2:1376603/150‑1 (MQ=255)

                                                  aGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTATCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGa                                                                                                  >  2:2878165/1‑150 (MQ=255)

                                                   gCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGAt                                                                                                 <  2:1693816/150‑1 (MQ=255)

                                                   gCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTATCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGAt                                                                                                 >  2:1668826/1‑150 (MQ=255)

                                                    cTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTATCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCCTTCTTCAGCAAGCGATa                                                                                                >  2:391116/1‑150 (MQ=255)

                                                      tCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATAcc                                                                                              >  1:1292160/1‑150 (MQ=255)

                                                       cAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATAccc                                                                                             >  2:410077/1‑150 (MQ=255)

                                                          ttCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTATCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGc                                                                                          >  2:768895/1‑150 (MQ=255)

                                                            ccAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCAc                                                                                        <  2:576621/150‑1 (MQ=255)

                                                             cAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACg                                                                                       >  1:3439447/1‑150 (MQ=255)

                                                               gTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTa                                                                                     <  2:2331352/150‑1 (MQ=255)

                                                               gTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTa                                                                                     <  1:1124433/150‑1 (MQ=255)

                                                                 aaTGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAAt                                                                                   <  2:3119871/150‑1 (MQ=255)

                                                                     aCAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCg                                                                               >  2:2288453/1‑150 (MQ=255)

                                                                      cAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCgg                                                                              >  2:4300982/1‑150 (MQ=255)

                                                                      cAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCgg                                                                              >  2:541430/1‑150 (MQ=255)

                                                                       aaTCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCGTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGt                                                                             >  2:3277198/1‑150 (MQ=255)

                                                                         tCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTgg                                                                           <  1:1392686/150‑1 (MQ=255)

                                                                           gACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCa                                                                         <  1:1719983/150‑1 (MQ=255)

                                                                            aCGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCAc                                                                        <  2:792260/150‑1 (MQ=255)

                                                                                cTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAAGTCCGGTGGCACGTga                                                                    <  2:101254/150‑1 (MQ=255)

                                                                                 tAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTgag                                                                   >  2:2236356/1‑150 (MQ=255)

                                                                                   aCCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGaa                                                                 <  2:182768/150‑1 (MQ=255)

                                                                                   aCCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGaa                                                                 >  2:3097574/1‑150 (MQ=255)

                                                                                     ctctTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAatc                                                               >  1:2295090/1‑150 (MQ=255)

                                                                                     ctctTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAatc                                                               >  1:529532/1‑150 (MQ=255)

                                                                                     ctctTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAatc                                                               <  1:3382920/150‑1 (MQ=255)

                                                                                     ctctTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTATCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAatc                                                               >  2:4525766/1‑150 (MQ=255)

                                                                                            tatTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATcc                                                        >  2:289593/1‑150 (MQ=255)

                                                                                                  ggATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATc                                                  >  1:2981006/1‑150 (MQ=255)

                                                                                                  ggATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATc                                                  >  1:4135989/1‑150 (MQ=255)

                                                                                                    atcatcGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCACGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCaa                                                >  1:4412520/1‑150 (MQ=255)

                                                                                                        tcGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTATCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGt                                            <  2:414303/150‑1 (MQ=255)

                                                                                                           tGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTcg                                         >  2:2633674/1‑150 (MQ=255)

                                                                                                           tGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTcg                                         <  1:3735058/150‑1 (MQ=255)

                                                                                                             aCGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTcgcc                                       <  2:2405232/150‑1 (MQ=255)

                                                                                                             aCGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTcgcc                                       <  1:797218/150‑1 (MQ=255)

                                                                                                             aCGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTcgcc                                       <  2:1323745/150‑1 (MQ=255)

                                                                                                               ggATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTcgccgc                                     >  1:1379055/1‑150 (MQ=255)

                                                                                                                gATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCt                                    >  2:1516742/1‑150 (MQ=255)

                                                                                                                gATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCt                                    <  1:1884605/150‑1 (MQ=255)

                                                                                                                    ccTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCt                                >  2:3776458/1‑150 (MQ=255)

                                                                                                                        tGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCa                            >  1:3230829/1‑150 (MQ=255)

                                                                                                                          agaATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAgg                          >  1:1493896/1‑150 (MQ=255)

                                                                                                                          agaATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAgg                          >  2:1198674/1‑150 (MQ=255)

                                                                                                                            aaTATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGtt                        >  1:1015426/1‑150 (MQ=255)

                                                                                                                              tatCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTAt                      <  1:2068190/150‑1 (MQ=255)

                                                                                                                              tatCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTAt                      >  2:438546/1‑150 (MQ=255)

                                                                                                                               atCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATc                     >  1:2762272/1‑150 (MQ=255)

                                                                                                                                    gAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACt                <  1:2389659/150‑1 (MQ=255)

                                                                                                                                       gCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCAc             >  2:560648/1‑150 (MQ=255)

                                                                                                                                        cTGAATTGCTTATCTCCGTTGCTCAAGTTCTGGCGGTACGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCAcc            >  2:380443/1‑150 (MQ=255)

                                                                                                                                        cTGAATTGCTTATCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCAcc            >  2:827966/1‑150 (MQ=255)

                                                                                                                                         tGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCt           >  1:1760087/1‑150 (MQ=255)

                                                                                                                                             ttGCTTCTCTCCGTTGCTCAATTTCTGGCGGTAAGAAACCGGCATTCTTCAGAAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCg       >  1:3364812/1‑150 (MQ=255)

                                                                                                                                               gCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCt     >  1:612188/1‑150 (MQ=255)

                                                                                                                                                 ttCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTg   <  1:159525/150‑1 (MQ=255)

                                                                                                                                                 ttCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTg   >  2:2314466/1‑150 (MQ=255)

                                                                                                                                                  tctctcCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTgc  >  1:3295889/1‑150 (MQ=255)

TGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGC  >  CP009273/3432529‑3432824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`