Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,473,139 G→T 100% I276I (ATC→ATA)  kefB ← potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,473,1390GT95.8% 819.9 / 25.8 260I276I (ATC→ATA) kefBpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (8/3);  new base T (113/136);  total (121/139)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 267 total aligned reads displayed.

TGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAA  >  CP009273/3472993‑3473286
                                                                                                                                                  |                                                                                                                                                   
tGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAtata                                                                                                                                                  >  2:4430398/1‑150 (MQ=255)
tGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAtata                                                                                                                                                  <  2:1674910/150‑1 (MQ=255)
  cATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATaaa                                                                                                                                                >  2:3523643/1‑150 (MQ=255)
    tCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGa                                                                                                                                              <  1:1251812/150‑1 (MQ=255)
    tCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGa                                                                                                                                              <  1:2821865/150‑1 (MQ=255)
     ccGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTCTAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGaa                                                                                                                                             >  2:17652/1‑150 (MQ=255)
      cGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAAc                                                                                                                                            <  2:4508749/150‑1 (MQ=255)
        ctctGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACaa                                                                                                                                          >  2:403803/1‑150 (MQ=255)
         tctGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGTGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAATAACaaa                                                                                                                                         <  2:3776103/150‑1 (MQ=255)
         tctGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACaaa                                                                                                                                         >  2:1137458/1‑150 (MQ=255)
          ctGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAAc                                                                                                                                        >  2:3592007/1‑150 (MQ=255)
           tGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAAcc                                                                                                                                       <  1:1708766/150‑1 (MQ=255)
             aGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGa                                                                                                                                     >  2:25914/1‑150 (MQ=255)
             aGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCAACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGa                                                                                                                                     <  2:93519/150‑1 (MQ=255)
              gCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGag                                                                                                                                    <  1:4434578/150‑1 (MQ=255)
              gCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGag                                                                                                                                    <  1:1668216/150‑1 (MQ=255)
               cTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGagc                                                                                                                                   >  1:1972125/1‑150 (MQ=255)
                tACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCAGAACAGTTGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATACAGAACTAACCGagca                                                                                                                                  <  2:1584767/150‑1 (MQ=255)
                tACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGagca                                                                                                                                  <  1:3192123/150‑1 (MQ=255)
                tACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGagca                                                                                                                                  <  1:4580424/150‑1 (MQ=255)
                 acgcacgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGagcag                                                                                                                                 <  2:4581140/150‑1 (MQ=255)
                   gcacgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAgcagca                                                                                                                               >  2:2778635/1‑150 (MQ=255)
                     acgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAg                                                                                                                             <  1:1812836/150‑1 (MQ=255)
                         cATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCtt                                                                                                                         >  1:3065909/1‑150 (MQ=255)
                         cATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCtt                                                                                                                         >  2:3046226/1‑150 (MQ=255)
                          aTACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCttt                                                                                                                        <  2:3168205/150‑1 (MQ=255)
                          aTACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCttt                                                                                                                        <  2:1415401/150‑1 (MQ=255)
                                tCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAggg                                                                                                                  <  2:3005079/150‑1 (MQ=255)
                                   cgcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATc                                                                                                               <  2:392797/150‑1 (MQ=255)
                                   cgcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATc                                                                                                               <  1:4140385/150‑1 (MQ=255)
                                    gcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCg                                                                                                              <  1:2483638/150‑1 (MQ=255)
                                    gcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGAGCg                                                                                                              >  2:3109306/1‑150 (MQ=255)
                                     ccAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGa                                                                                                             >  2:2445141/1‑150 (MQ=255)
                                      cagcagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGAt                                                                                                            <  1:4026157/150‑1 (MQ=255)
                                      cagcagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGAt                                                                                                            <  1:808349/150‑1 (MQ=255)
                                         cagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGc                                                                                                         >  1:4191072/1‑150 (MQ=255)
                                         cagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGc                                                                                                         >  2:698632/1‑150 (MQ=255)
                                         cagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGc                                                                                                         <  1:824063/150‑1 (MQ=255)
                                          agATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGcc                                                                                                        <  2:2385222/150‑1 (MQ=255)
                                             tACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACCGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGtt                                                                                                     <  2:2842845/150‑1 (MQ=255)
                                             tACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGtt                                                                                                     <  2:3501598/150‑1 (MQ=255)
                                              aCAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGttt                                                                                                    <  1:3551071/150‑1 (MQ=255)
                                                  cACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAg                                                                                                <  1:1081122/150‑1 (MQ=255)
                                                        aaTTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATg                                                                                          <  2:2640176/150‑1 (MQ=255)
                                                         aTTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATgg                                                                                         <  1:4495452/150‑1 (MQ=255)
                                                         aTTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATgg                                                                                         <  2:1008880/150‑1 (MQ=255)
                                                              cACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGata                                                                                    <  1:1008225/150‑1 (MQ=255)
                                                              cACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGata                                                                                    <  1:1008219/150‑1 (MQ=255)
                                                              cACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGata                                                                                    >  1:3743515/1‑150 (MQ=255)
                                                                 cGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTc                                                                                 >  2:3419779/1‑150 (MQ=255)
                                                                  gCCACCTGCTCAACCACGCTTATCACTACCCACACCAGATGGGTATTAAGCACCCGGAGGTTGAGTGTCATGCCGTCATATTTAATGATCTAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCa                                                                                <  2:792373/150‑1 (MQ=255)
                                                                  gCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCa                                                                                >  2:454457/1‑150 (MQ=255)
                                                                   ccaccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCAc                                                                               >  1:1604247/1‑150 (MQ=255)
                                                                     accaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACtt                                                                             >  2:3397843/1‑150 (MQ=255)
                                                                      ccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACttt                                                                            >  1:334621/1‑150 (MQ=255)
                                                                        aGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTcc                                                                          >  1:645037/1‑150 (MQ=255)
                                                                         gCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCg                                                                         <  1:2358051/150‑1 (MQ=255)
                                                                           acaACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGcc                                                                       <  2:1291464/150‑1 (MQ=255)
                                                                           acaACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGcc                                                                       <  2:3851173/150‑1 (MQ=255)
                                                                           acaACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGcc                                                                       >  2:2464731/1‑150 (MQ=255)
                                                                             aaCCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcag                                                                     >  2:3519752/1‑150 (MQ=255)
                                                                             aaCCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcag                                                                     >  2:1540285/1‑150 (MQ=255)
                                                                               ccACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcagca                                                                   >  1:986934/1‑150 (MQ=255)
                                                                               ccACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGAAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcagca                                                                   <  1:837760/150‑1 (MQ=255)
                                                                                cACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcagcag                                                                  >  2:1989008/1‑150 (MQ=255)
                                                                                cACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcagcag                                                                  >  1:559871/1‑150 (MQ=255)
                                                                                 aCGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCagcagc                                                                 <  2:544918/150‑1 (MQ=255)
                                                                                  cGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAgcagca                                                                <  1:279669/150‑1 (MQ=255)
                                                                                  cGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAgcagca                                                                <  1:2703447/150‑1 (MQ=255)
                                                                                    cTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACg                                                              <  1:3408844/150‑1 (MQ=255)
                                                                                      tATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGcc                                                            >  1:428429/1‑150 (MQ=255)
                                                                                       aTCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGccc                                                           <  1:3591993/150‑1 (MQ=255)
                                                                                        tCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCg                                                          >  1:1666933/1‑150 (MQ=255)
                                                                                            tACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAAt                                                      >  2:2336527/1‑150 (MQ=255)
                                                                                                      aGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCg                                            >  1:553583/1‑150 (MQ=255)
                                                                                                      aGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCg                                            >  1:2440121/1‑150 (MQ=255)
                                                                                                             tataAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCa                                     >  1:2197413/1‑150 (MQ=255)
                                                                                                               taAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATc                                   <  2:4189624/150‑1 (MQ=255)
                                                                                                                aaaGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCg                                  <  2:533444/150‑1 (MQ=255)
                                                                                                                 aaGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGa                                 >  2:1199057/1‑150 (MQ=255)
                                                                                                                   gCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACa                               <  2:108422/150‑1 (MQ=255)
                                                                                                                     aCCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGc                             >  1:2706921/1‑150 (MQ=255)
                                                                                                                        ccGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGcccc                          <  1:1256746/150‑1 (MQ=255)
                                                                                                                          gAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAg                        >  2:3583022/1‑150 (MQ=255)
                                                                                                                           aGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgc                       <  2:2114751/150‑1 (MQ=255)
                                                                                                                           aGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgc                       >  2:1751645/1‑150 (MQ=255)
                                                                                                                            ggTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcg                      <  2:2906602/150‑1 (MQ=255)
                                                                                                                                  gTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCa                >  1:3652306/1‑150 (MQ=255)
                                                                                                                                   tGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCAt               <  1:929859/150‑1 (MQ=255)
                                                                                                                                     aCATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATaa             <  1:2297985/150‑1 (MQ=255)
                                                                                                                                     aCATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATaa             >  1:2761058/1‑150 (MQ=255)
                                                                                                                                       aTGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAAc           <  1:3874188/150‑1 (MQ=255)
                                                                                                                                         gCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACaa         <  1:25914/150‑1 (MQ=255)
                                                                                                                                         gCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACaa         >  2:4392951/1‑150 (MQ=255)
                                                                                                                                          ccGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAAt        <  2:3301539/150‑1 (MQ=255)
                                                                                                                                           cGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATg       <  2:107087/150‑1 (MQ=255)
                                                                                                                                           cGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATg       >  1:2629151/1‑150 (MQ=255)
                                                                                                                                             aCAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCg     >  2:3760235/1‑150 (MQ=255)
                                                                                                                                               agatATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGa   <  2:4137970/150‑1 (MQ=255)
                                                                                                                                                gagaTAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGaa  >  2:3396162/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAA  >  CP009273/3472993‑3473286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑