Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,494,830 C→T 34.1% R68R (CGC→CGT)  frlB → fructoselysine‑6‑P‑deglycase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,494,8300CT34.1% 403.8 / 296.0 293R68R (CGC→CGT) frlBfructoselysine‑6‑P‑deglycase
Reads supporting (aligned to +/- strand):  ref base C (88/105);  new base T (38/62);  total (126/167)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

CAGGAAGTGGAAAAAGTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAGCGCTGGAGCTGGGCCGGGCCTG  >  CP009273/3494681‑3494979
                                                                                                                                                     |                                                                                                                                                     
  ggAAGTGGAAAAAGTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCtt                                                                                                                                                     <  2:297717/150‑1 (MQ=255)
    aaGTGGAAAAAGTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCtttt                                                                                                                                                   <  1:3896113/150‑1 (MQ=255)
     aGTGGAAAAAGTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTc                                                                                                                                                  <  1:217311/150‑1 (MQ=255)
      gTGGAAAAAGTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTcc                                                                                                                                                 >  1:1541496/1‑150 (MQ=255)
       tGGAAAAAGTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCg                                                                                                                                                >  2:3050678/1‑150 (MQ=255)
         gAAAAAGTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGAt                                                                                                                                              <  1:425737/150‑1 (MQ=255)
         gAAAAAGTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGAt                                                                                                                                              <  2:2858370/150‑1 (MQ=255)
           aaaaGTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCt                                                                                                                                            <  2:2730296/150‑1 (MQ=255)
            aaaGTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCtt                                                                                                                                           <  1:1033719/150‑1 (MQ=255)
            aaaGTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCtt                                                                                                                                           >  1:2071408/1‑150 (MQ=255)
              aGTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCa                                                                                                                                         >  2:334563/1‑150 (MQ=255)
              aGTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCa                                                                                                                                         <  2:2792893/150‑1 (MQ=255)
               gTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAg                                                                                                                                        <  1:1360217/150‑1 (MQ=255)
               gTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAg                                                                                                                                        <  1:2457668/150‑1 (MQ=255)
                 tctcAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGt                                                                                                                                      >  2:1141443/1‑150 (MQ=255)
                  ctcAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTc                                                                                                                                     >  1:4328311/1‑150 (MQ=255)
                  ctcAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTc                                                                                                                                     >  1:3385289/1‑150 (MQ=255)
                   tcAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCt                                                                                                                                    <  1:282820/150‑1 (MQ=255)
                    cAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAATGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTa                                                                                                                                   <  2:1750491/150‑1 (MQ=255)
                    cAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTa                                                                                                                                   >  1:2220397/1‑150 (MQ=255)
                     aGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTAc                                                                                                                                  >  1:3016388/1‑150 (MQ=255)
                        cATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGcc                                                                                                                               <  1:967135/150‑1 (MQ=255)
                        cATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGcc                                                                                                                               <  1:554521/150‑1 (MQ=255)
                            aCGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCAttt                                                                                                                           <  2:1041284/150‑1 (MQ=255)
                             cGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTc                                                                                                                          <  1:4430020/150‑1 (MQ=255)
                                tCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCgg                                                                                                                       >  2:1430114/1‑150 (MQ=255)
                                tCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCgg                                                                                                                       <  1:3476896/150‑1 (MQ=255)
                                 ccGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGc                                                                                                                      <  2:2115569/150‑1 (MQ=255)
                                   gCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTg                                                                                                                    >  2:2249994/1‑150 (MQ=255)
                                    cTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTgg                                                                                                                   <  1:520321/150‑1 (MQ=255)
                                      ggTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGa                                                                                                                 >  1:903529/1‑150 (MQ=255)
                                       gTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAg                                                                                                                <  1:665236/150‑1 (MQ=255)
                                           aCGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCt                                                                                                            >  2:3624095/1‑150 (MQ=255)
                                             gCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGc                                                                                                          <  1:3360123/150‑1 (MQ=255)
                                             gCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGc                                                                                                          >  2:3956341/1‑150 (MQ=255)
                                              ccATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCg                                                                                                         >  2:1504049/1‑150 (MQ=255)
                                                aTCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGAt                                                                                                       >  2:3164100/1‑150 (MQ=255)
                                                     ggAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAacac                                                                                                  >  2:323115/1‑150 (MQ=255)
                                                     ggAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAacac                                                                                                  >  2:1337089/1‑150 (MQ=255)
                                                      gAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACAcc                                                                                                 >  2:2194744/1‑150 (MQ=255)
                                                      gAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTACCCATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACAcc                                                                                                 >  2:4528657/1‑150 (MQ=255)
                                                        agagATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACAcccc                                                                                               >  2:2777249/1‑150 (MQ=255)
                                                         gagaTGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCg                                                                                              <  1:1530554/150‑1 (MQ=255)
                                                          agaTGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGt                                                                                             >  1:60206/1‑150 (MQ=255)
                                                             tGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGTCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATc                                                                                          >  2:90415/1‑150 (MQ=255)
                                                             tGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATc                                                                                          <  2:958613/150‑1 (MQ=255)
                                                             tGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATc                                                                                          <  1:4224336/150‑1 (MQ=255)
                                                             tGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATc                                                                                          <  1:1200462/150‑1 (MQ=255)
                                                                  aaGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTc                                                                                     <  1:1777977/150‑1 (MQ=255)
                                                                   aGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTcg                                                                                    >  2:2899493/1‑150 (MQ=255)
                                                                     cgcgACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTcgac                                                                                  <  2:797459/150‑1 (MQ=255)
                                                                     cgcgACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTcgac                                                                                  <  1:2331602/150‑1 (MQ=255)
                                                                     cgcgACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTcgac                                                                                  >  2:1378401/1‑150 (MQ=255)
                                                                     cgcgACATTGATCGTATTTATTTCGTAGCATGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTcgac                                                                                  >  1:3299963/1‑150 (MQ=255)
                                                                         aCATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGATGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATc                                                                              <  1:3072491/150‑1 (MQ=255)
                                                                         aCATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATc                                                                              <  1:4613995/150‑1 (MQ=255)
                                                                            ttGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGtt                                                                           <  1:3979349/150‑1 (MQ=255)
                                                                               aTCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTgcg                                                                        <  1:2633030/150‑1 (MQ=255)
                                                                                 cGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCa                                                                      >  2:2640739/1‑150 (MQ=255)
                                                                                   tatttattTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGt                                                                    <  2:1912710/150‑1 (MQ=255)
                                                                                   tatttattTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGt                                                                    <  1:4299346/150‑1 (MQ=255)
                                                                                    atttatttCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTa                                                                   >  2:1867390/1‑150 (MQ=255)
                                                                                       tatttCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAAtt                                                                <  1:1660419/150‑1 (MQ=255)
                                                                                       tatttCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAAtt                                                                >  2:3862761/1‑150 (MQ=255)
                                                                                         tttCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTgg                                                              <  1:2149733/150‑1 (MQ=255)
                                                                                         tttCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTgg                                                              <  1:839313/150‑1 (MQ=255)
                                                                                          ttCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAAAATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGc                                                             <  2:2408479/150‑1 (MQ=255)
                                                                                          ttCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAAAATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGc                                                             <  2:566228/150‑1 (MQ=255)
                                                                                            cGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGt                                                           >  1:2929039/1‑150 (MQ=255)
                                                                                             gTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGtt                                                          >  1:3331935/1‑150 (MQ=255)
                                                                                             gTTGCCTGCGGATCGCCACTCAACGCGGCGCAAAAGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGtt                                                          >  2:3196176/1‑150 (MQ=255)
                                                                                              ttGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGttt                                                         >  1:3809136/1‑150 (MQ=255)
                                                                                                gCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCt                                                       <  2:576551/150‑1 (MQ=255)
                                                                                                 ccTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTg                                                      <  2:2890531/150‑1 (MQ=255)
                                                                                                   tGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGAc                                                    <  1:3601697/150‑1 (MQ=255)
                                                                                                     cGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTa                                                  >  1:1002072/1‑150 (MQ=255)
                                                                                                      ggATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTTGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTAc                                                 <  2:732167/150‑1 (MQ=255)
                                                                                                              aCTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAAc                                         >  1:4519874/1‑150 (MQ=255)
                                                                                                               cTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAAcc                                        <  1:487254/150‑1 (MQ=255)
                                                                                                               cTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAAcc                                        <  1:16200/150‑1 (MQ=255)
                                                                                                                 cAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGa                                      <  1:253612/150‑1 (MQ=255)
                                                                                                                  aaCGCGGCGCAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGaa                                     >  1:2266983/1‑150 (MQ=255)
                                                                                                                        gcgcAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTa                               <  2:3560685/150‑1 (MQ=255)
                                                                                                                         cgcAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTaa                              <  2:3559292/150‑1 (MQ=255)
                                                                                                                          gcAAACGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAAt                             >  2:3013456/1‑150 (MQ=255)
                                                                                                                            aaaCGGCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCa                           >  2:2670545/1‑150 (MQ=255)
                                                                                                                               cGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAg                        <  2:1673750/150‑1 (MQ=255)
                                                                                                                                ggCGAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAgc                       <  2:3430553/150‑1 (MQ=255)
                                                                                                                                   gAAACATCTGGCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAGCGCt                    >  1:3843627/1‑150 (MQ=255)
                                                                                                                                   gAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAGCGCt                    >  2:1538372/1‑150 (MQ=255)
                                                                                                                                      aCATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAGCGCTGGa                 >  2:3953867/1‑150 (MQ=255)
                                                                                                                                      aCATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAGCGCTGGa                 >  2:124074/1‑150 (MQ=255)
                                                                                                                                         tCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAGCGCTGGAGCt              <  1:4098762/150‑1 (MQ=255)
                                                                                                                                           tGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAGCGCTGGAGCTgg            <  1:2777249/150‑1 (MQ=255)
                                                                                                                                            ggCGGATCGTTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAGCGCTGGAGCTggg           <  2:3879001/150‑1 (MQ=255)
                                                                                                                                            ggCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAGCGCTGGAGCTggg           <  2:1046809/150‑1 (MQ=255)
                                                                                                                                            ggCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAGCGCTGGAGCTggg           <  1:3135630/150‑1 (MQ=255)
                                                                                                                                                 aTCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAGCGCTGGAGCTgggccggg      <  2:4328311/150‑1 (MQ=255)
                                                                                                                                                     cTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAGCGCTGGAGCTGGGCCGGGCCTg  >  2:1980984/1‑150 (MQ=255)
                                                                                                                                                     cTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAGCGCTGGAGCTGGGCCGGGCCTg  <  1:3164123/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGGAAGTGGAAAAAGTTCTCAGCCATGACGTTCCGCTGGTGCACGCCATCGTGGAAGAGATGGTGAAGCGCGACATTGATCGTATTTATTTCGTTGCCTGCGGATCGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCGGATCGCTTTTCCGATCTTCAGGTCTACGCCATTTCCGGCTGGGAGTTCTGCGATAACACCCCGTATCGCCTCGACGATCGTTGCGCAGTAATTGGCGTTTCTGACTACGGTAAAACCGAAGAGGTAATCAAAGCGCTGGAGCTGGGCCGGGCCTG  >  CP009273/3494681‑3494979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑